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Protein

Protein GrpE

Gene

grpE

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.UniRule annotation

Cofactori

K+Note: Required to stimulate the ATPase activity of DnaK.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Names & Taxonomyi

Protein namesi
Recommended name:
Protein GrpEUniRule annotation
Alternative name(s):
HSP-70 cofactorUniRule annotation
Gene namesi
Name:grpEUniRule annotation
Ordered Locus Names:TTHA1490
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001138841 – 177Protein GrpEAdd BLAST177

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi300852.TTHA1490.

Structurei

Secondary structure

1177
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni5 – 7Combined sources3
Beta strandi8 – 10Combined sources3
Helixi11 – 14Combined sources4
Turni15 – 17Combined sources3
Helixi18 – 21Combined sources4
Turni24 – 26Combined sources3
Helixi30 – 33Combined sources4
Beta strandi35 – 37Combined sources3
Helixi38 – 43Combined sources6
Turni44 – 51Combined sources8
Helixi52 – 87Combined sources36
Helixi89 – 91Combined sources3
Helixi93 – 113Combined sources21
Turni128 – 130Combined sources3
Beta strandi131 – 140Combined sources10
Beta strandi143 – 149Combined sources7
Beta strandi152 – 154Combined sources3
Beta strandi157 – 160Combined sources4
Beta strandi162 – 168Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3A6MX-ray3.23A/B1-177[»]
ProteinModelPortaliQ56236.
SMRiQ56236.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ56236.

Family & Domainsi

Sequence similaritiesi

Belongs to the GrpE family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105K90. Bacteria.
COG0576. LUCA.
HOGENOMiHOG000252083.
KOiK03687.
OMAiFANENIM.
PhylomeDBiQ56236.

Family and domain databases

CDDicd00446. GrpE. 1 hit.
Gene3Di2.30.22.10. 1 hit.
3.90.20.20. 1 hit.
HAMAPiMF_01151. GrpE. 1 hit.
InterProiIPR000740. GrpE.
IPR013805. GrpE_coiled_coil.
IPR009012. GrpE_head.
[Graphical view]
PANTHERiPTHR21237. PTHR21237. 1 hit.
PfamiPF01025. GrpE. 1 hit.
[Graphical view]
PRINTSiPR00773. GRPEPROTEIN.
SUPFAMiSSF51064. SSF51064. 1 hit.
PROSITEiPS01071. GRPE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q56236-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEERNHENTL EKDLEAVGQE AQALEERLKA AEEELKGLKD KYLRLLADFD
60 70 80 90 100
NYRKRMEEEL KAREREGVLK ALRALLPVLD DLDRALEFAE ASPESIRQGV
110 120 130 140 150
RAIRDGFFRI LAGLGVEEVP GEGEAFDPRY HEAVGLLPGE PGKVAKVFQR
160 170
GFRMGEALVR PARVAVGEEK REEADLE
Length:177
Mass (Da):20,027
Last modified:November 1, 1997 - v1
Checksum:i50AB3E3AA00DB17A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18 – 22GQEAQ → ARRPM in BAA12281 (PubMed:8663379).Curated5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L57504 Genomic DNA. Translation: AAB04677.1.
D84222 Genomic DNA. Translation: BAA12281.1.
Y07826 Genomic DNA. Translation: CAA69160.1.
AB012390 Genomic DNA. Translation: BAA81742.1.
AB032368 Genomic DNA. Translation: BAA96088.1.
AP008226 Genomic DNA. Translation: BAD71313.1.
RefSeqiWP_011228714.1. NC_006461.1.
YP_144756.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD71313; BAD71313; BAD71313.
GeneIDi3169984.
KEGGittj:TTHA1490.
PATRICi23957941. VBITheThe93045_1465.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L57504 Genomic DNA. Translation: AAB04677.1.
D84222 Genomic DNA. Translation: BAA12281.1.
Y07826 Genomic DNA. Translation: CAA69160.1.
AB012390 Genomic DNA. Translation: BAA81742.1.
AB032368 Genomic DNA. Translation: BAA96088.1.
AP008226 Genomic DNA. Translation: BAD71313.1.
RefSeqiWP_011228714.1. NC_006461.1.
YP_144756.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3A6MX-ray3.23A/B1-177[»]
ProteinModelPortaliQ56236.
SMRiQ56236.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA1490.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD71313; BAD71313; BAD71313.
GeneIDi3169984.
KEGGittj:TTHA1490.
PATRICi23957941. VBITheThe93045_1465.

Phylogenomic databases

eggNOGiENOG4105K90. Bacteria.
COG0576. LUCA.
HOGENOMiHOG000252083.
KOiK03687.
OMAiFANENIM.
PhylomeDBiQ56236.

Miscellaneous databases

EvolutionaryTraceiQ56236.

Family and domain databases

CDDicd00446. GrpE. 1 hit.
Gene3Di2.30.22.10. 1 hit.
3.90.20.20. 1 hit.
HAMAPiMF_01151. GrpE. 1 hit.
InterProiIPR000740. GrpE.
IPR013805. GrpE_coiled_coil.
IPR009012. GrpE_head.
[Graphical view]
PANTHERiPTHR21237. PTHR21237. 1 hit.
PfamiPF01025. GrpE. 1 hit.
[Graphical view]
PRINTSiPR00773. GRPEPROTEIN.
SUPFAMiSSF51064. SSF51064. 1 hit.
PROSITEiPS01071. GRPE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGRPE_THET8
AccessioniPrimary (citable) accession number: Q56236
Secondary accession number(s): P74895, Q5SI84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.