Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein GrpE

Gene

grpE

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.UniRule annotation

Cofactori

K+Note: Required to stimulate the ATPase activity of DnaK.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-1527-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein GrpEUniRule annotation
Alternative name(s):
HSP-70 cofactorUniRule annotation
Gene namesi
Name:grpEUniRule annotation
Ordered Locus Names:TTHA1490
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 177177Protein GrpEPRO_0000113884Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi300852.TTHA1490.

Structurei

Secondary structure

1
177
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni5 – 73Combined sources
Beta strandi8 – 103Combined sources
Helixi11 – 144Combined sources
Turni15 – 173Combined sources
Helixi18 – 214Combined sources
Turni24 – 263Combined sources
Helixi30 – 334Combined sources
Beta strandi35 – 373Combined sources
Helixi38 – 436Combined sources
Turni44 – 518Combined sources
Helixi52 – 8736Combined sources
Helixi89 – 913Combined sources
Helixi93 – 11321Combined sources
Turni128 – 1303Combined sources
Beta strandi131 – 14010Combined sources
Beta strandi143 – 1497Combined sources
Beta strandi152 – 1543Combined sources
Beta strandi157 – 1604Combined sources
Beta strandi162 – 1687Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3A6MX-ray3.23A/B1-177[»]
ProteinModelPortaliQ56236.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ56236.

Family & Domainsi

Sequence similaritiesi

Belongs to the GrpE family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105K90. Bacteria.
COG0576. LUCA.
HOGENOMiHOG000252083.
KOiK03687.
OMAiVCDSLDA.
OrthoDBiEOG6FJNJ9.
PhylomeDBiQ56236.

Family and domain databases

Gene3Di2.30.22.10. 1 hit.
3.90.20.20. 1 hit.
HAMAPiMF_01151. GrpE.
InterProiIPR000740. GrpE.
IPR013805. GrpE_coiled_coil.
IPR009012. GrpE_head.
[Graphical view]
PANTHERiPTHR21237. PTHR21237. 1 hit.
PfamiPF01025. GrpE. 1 hit.
[Graphical view]
PRINTSiPR00773. GRPEPROTEIN.
SUPFAMiSSF51064. SSF51064. 1 hit.
PROSITEiPS01071. GRPE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q56236-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEERNHENTL EKDLEAVGQE AQALEERLKA AEEELKGLKD KYLRLLADFD
60 70 80 90 100
NYRKRMEEEL KAREREGVLK ALRALLPVLD DLDRALEFAE ASPESIRQGV
110 120 130 140 150
RAIRDGFFRI LAGLGVEEVP GEGEAFDPRY HEAVGLLPGE PGKVAKVFQR
160 170
GFRMGEALVR PARVAVGEEK REEADLE
Length:177
Mass (Da):20,027
Last modified:November 1, 1997 - v1
Checksum:i50AB3E3AA00DB17A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti18 – 225GQEAQ → ARRPM in BAA12281 (PubMed:8663379).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L57504 Genomic DNA. Translation: AAB04677.1.
D84222 Genomic DNA. Translation: BAA12281.1.
Y07826 Genomic DNA. Translation: CAA69160.1.
AB012390 Genomic DNA. Translation: BAA81742.1.
AB032368 Genomic DNA. Translation: BAA96088.1.
AP008226 Genomic DNA. Translation: BAD71313.1.
RefSeqiWP_011228714.1. NC_006461.1.
YP_144756.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD71313; BAD71313; BAD71313.
GeneIDi3169984.
KEGGittj:TTHA1490.
PATRICi23957941. VBITheThe93045_1465.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L57504 Genomic DNA. Translation: AAB04677.1.
D84222 Genomic DNA. Translation: BAA12281.1.
Y07826 Genomic DNA. Translation: CAA69160.1.
AB012390 Genomic DNA. Translation: BAA81742.1.
AB032368 Genomic DNA. Translation: BAA96088.1.
AP008226 Genomic DNA. Translation: BAD71313.1.
RefSeqiWP_011228714.1. NC_006461.1.
YP_144756.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3A6MX-ray3.23A/B1-177[»]
ProteinModelPortaliQ56236.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA1490.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD71313; BAD71313; BAD71313.
GeneIDi3169984.
KEGGittj:TTHA1490.
PATRICi23957941. VBITheThe93045_1465.

Phylogenomic databases

eggNOGiENOG4105K90. Bacteria.
COG0576. LUCA.
HOGENOMiHOG000252083.
KOiK03687.
OMAiVCDSLDA.
OrthoDBiEOG6FJNJ9.
PhylomeDBiQ56236.

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-1527-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ56236.

Family and domain databases

Gene3Di2.30.22.10. 1 hit.
3.90.20.20. 1 hit.
HAMAPiMF_01151. GrpE.
InterProiIPR000740. GrpE.
IPR013805. GrpE_coiled_coil.
IPR009012. GrpE_head.
[Graphical view]
PANTHERiPTHR21237. PTHR21237. 1 hit.
PfamiPF01025. GrpE. 1 hit.
[Graphical view]
PRINTSiPR00773. GRPEPROTEIN.
SUPFAMiSSF51064. SSF51064. 1 hit.
PROSITEiPS01071. GRPE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequencing, and expression of dnaK-operon proteins from the thermophilic bacterium Thermus thermophilus."
    Osipiuk J., Joachimiak A.
    Biochim. Biophys. Acta 1353:253-265(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "A novel factor required for the assembly of the DnaK and DnaJ chaperones of Thermus thermophilus."
    Motohashi K., Yohda M., Endo I., Yoshida M.
    J. Biol. Chem. 271:17343-17348(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Seidel R.
    Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "K+ is an indispensable cofactor for GrpE stimulation of ATPase activity of DnaK/DnaJ complex from Thermus thermophilus."
    Motohashi K., Yohda M., Odaka M., Yoshida M.
    FEBS Lett. 412:633-636(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-8, POTASSIUM REQUIREMENT.
  5. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.

Entry informationi

Entry nameiGRPE_THET8
AccessioniPrimary (citable) accession number: Q56236
Secondary accession number(s): P74895, Q5SI84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 11, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.