Q56236 (GRPE_THET8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein GrpE

Alternative name(s):
HSP-70 cofactor

Gene names

Name:	grpE
Ordered Locus Names:	TTHA1490

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus › Thermus thermophilus

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding By similarity. HAMAP-Rule MF_01151
Cofactor	Potassium. Required to stimulate the ATPase activity of DnaK.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Probable HAMAP-Rule MF_01151.
Sequence similarities	Belongs to the GrpE family.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Molecular function	Chaperone
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	protein folding Inferred from electronic annotation. Source: InterPro response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	adenyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 177

177

Protein GrpE HAMAP-Rule MF_01151

PRO_0000113884

Experimental info

Sequence conflict

18 – 22

5

GQEAQ → ARRPM in BAA12281. Ref.2

Secondary structure

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177

Helix Strand Turn

Details...

Turn

5 – 7

3

Beta strand

8 – 10

3

Helix

11 – 14

4

Turn

15 – 17

3

Helix

18 – 21

4

Turn

24 – 26

3

Helix

30 – 33

4

Beta strand

35 – 37

3

Helix

38 – 43

6

Turn

44 – 51

8

Helix

52 – 87

36

Helix

89 – 91

3

Helix

93 – 113

21

Turn

128 – 130

3

Beta strand

131 – 140

10

Beta strand

143 – 149

7

Beta strand

152 – 154

3

Beta strand

157 – 160

4

Beta strand

162 – 168

7

Sequences

	Sequence		Length	Mass (Da)
	Q56236 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 50AB3E3AA00DB17A	FASTA	177	20,027
	10 20 30 40 50 60 MEERNHENTL EKDLEAVGQE AQALEERLKA AEEELKGLKD KYLRLLADFD NYRKRMEEEL 70 80 90 100 110 120 KAREREGVLK ALRALLPVLD DLDRALEFAE ASPESIRQGV RAIRDGFFRI LAGLGVEEVP 130 140 150 160 170 GEGEAFDPRY HEAVGLLPGE PGKVAKVFQR GFRMGEALVR PARVAVGEEK REEADLE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing, and expression of dnaK-operon proteins from the thermophilic bacterium Thermus thermophilus." Osipiuk J., Joachimiak A. Biochim. Biophys. Acta 1353:253-265(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A novel factor required for the assembly of the DnaK and DnaJ chaperones of Thermus thermophilus." Motohashi K., Yohda M., Endo I., Yoshida M. J. Biol. Chem. 271:17343-17348(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	Seidel R. Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"K+ is an indispensable cofactor for GrpE stimulation of ATPase activity of DnaK/DnaJ complex from Thermus thermophilus." Motohashi K., Yohda M., Odaka M., Yoshida M. FEBS Lett. 412:633-636(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-8, POTASSIUM REQUIREMENT.
[5]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HB8 / ATCC 27634 / DSM 579.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L57504 Genomic DNA. Translation: AAB04677.1.
D84222 Genomic DNA. Translation: BAA12281.1.
Y07826 Genomic DNA. Translation: CAA69160.1.
AB012390 Genomic DNA. Translation: BAA81742.1.
AB032368 Genomic DNA. Translation: BAA96088.1.
AP008226 Genomic DNA. Translation: BAD71313.1.

RefSeq

YP_144756.1. NC_006461.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3A6M	X-ray	3.23	A/B	1-177	[»]

ProteinModelPortal

Q56236.

ModBase

Search...

Protein-protein interaction databases

STRING

300852.TTHA1490.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

BAD71313; BAD71313; BAD71313.

GeneID

3169984.

KEGG

ttj:TTHA1490.

PATRIC

23957941. VBITheThe93045_1465.

Phylogenomic databases

eggNOG

COG0576.

HOGENOM

HOG000252083.

KO

K03687.

OMA

ERNHENT.

ProtClustDB

CLSK738530.

Family and domain databases

Gene3D

2.30.22.10. 1 hit.
3.90.20.20. 1 hit.

HAMAP

MF_01151. GrpE.

InterPro

IPR000740. GrpE.
IPR013805. GrpE_coiled_coil.
IPR009012. GrpE_head.
[Graphical view]

PANTHER

PTHR21237. PTHR21237. 1 hit.

Pfam

PF01025. GrpE. 1 hit.
[Graphical view]

PRINTS

PR00773. GRPEPROTEIN.

SUPFAM

SSF51064. GrpE_head. 1 hit.

PROSITE

PS01071. GRPE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q56236.

Entry information

Entry name

GRPE_THET8

Accession

Primary (citable) accession number: Q56236
Secondary accession number(s): P74895, Q5SI84

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program