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Q56229

- NQO14_THET8

UniProt

Q56229 - NQO14_THET8

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Protein

NADH-quinone oxidoreductase subunit 14

Gene

nqo14

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.

Catalytic activityi

NADH + quinone = NAD+ + quinol.UniRule annotation

GO - Molecular functioni

  1. NADH dehydrogenase (ubiquinone) activity Source: InterPro
  2. quinone binding Source: UniProtKB-KW

GO - Biological processi

  1. ATP synthesis coupled electron transport Source: InterPro
  2. transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Transport

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-101-MONOMER.

Protein family/group databases

TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit 14 (EC:1.6.99.5UniRule annotation)
Alternative name(s):
NADH dehydrogenase I chain 14
NDH-1 subunit 14
Gene namesi
Name:nqo14
Ordered Locus Names:TTHA0097
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
ProteomesiUP000000532: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1 – 2121HelicalUniRule annotationAdd
BLAST
Transmembranei30 – 5021HelicalUniRule annotationAdd
BLAST
Transmembranei57 – 7721HelicalUniRule annotationAdd
BLAST
Transmembranei79 – 9921HelicalUniRule annotationAdd
BLAST
Transmembranei104 – 12421HelicalUniRule annotationAdd
BLAST
Transmembranei137 – 15721HelicalUniRule annotationAdd
BLAST
Transmembranei172 – 19221HelicalUniRule annotationAdd
BLAST
Transmembranei204 – 22421HelicalUniRule annotationAdd
BLAST
Transmembranei230 – 25021HelicalUniRule annotationAdd
BLAST
Transmembranei257 – 27721HelicalUniRule annotationAdd
BLAST
Transmembranei280 – 30021HelicalUniRule annotationAdd
BLAST
Transmembranei322 – 34221HelicalUniRule annotationAdd
BLAST
Transmembranei360 – 38021HelicalUniRule annotationAdd
BLAST
Transmembranei400 – 42021HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 427427NADH-quinone oxidoreductase subunit 14PRO_0000117687Add
BLAST

Keywords - PTMi

Quinone

Interactioni

Subunit structurei

NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.

Protein-protein interaction databases

DIPiDIP-59272N.
STRINGi300852.TTHA0097.

Structurei

Secondary structure

1
427
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 1615Combined sources
Turni17 – 193Combined sources
Helixi22 – 4221Combined sources
Turni43 – 453Combined sources
Beta strandi51 – 566Combined sources
Helixi57 – 7519Combined sources
Helixi83 – 9917Combined sources
Helixi104 – 12118Combined sources
Helixi127 – 15731Combined sources
Helixi170 – 18617Combined sources
Helixi195 – 2028Combined sources
Helixi205 – 22521Combined sources
Helixi230 – 24920Combined sources
Helixi254 – 26916Combined sources
Helixi270 – 2734Combined sources
Turni274 – 2763Combined sources
Helixi279 – 30224Combined sources
Helixi309 – 3124Combined sources
Helixi315 – 3184Combined sources
Helixi320 – 33415Combined sources
Helixi340 – 35516Combined sources
Helixi358 – 37013Combined sources
Helixi372 – 3798Combined sources
Helixi381 – 3833Combined sources
Helixi396 – 41419Combined sources
Turni416 – 4194Combined sources
Helixi420 – 4223Combined sources
Beta strandi423 – 4253Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HE8X-ray3.30I/N1-427[»]
4HEAX-ray3.30N/V1-427[»]
ProteinModelPortaliQ56229.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I subunit 2 family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1007.
HOGENOMiHOG000100794.
KOiK00343.
OMAiRIIIALY.
OrthoDBiEOG64JFNZ.
PhylomeDBiQ56229.

Family and domain databases

HAMAPiMF_00445. NDH1_NuoN_1.
InterProiIPR010096. NADH-Q_OxRdtase_suN/2.
IPR001750. NADH_UbQ/plastoQ_OxRdtase.
[Graphical view]
PfamiPF00361. Oxidored_q1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q56229-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTLAILAVFS VALTLLGFVL PPQGVKRATL LGLALALASL LLTWGKPFAF
60 70 80 90 100
GPYAVDGVSQ VFTLLALLGA LWTVGLVRSG RFEFYLLVLY AALGMHLLAS
110 120 130 140 150
TRHLLLMLVA LEALSLPLYA LATWRRGQGL EAALKYFLLG ALAAAFFLYG
160 170 180 190 200
AALFYGATGS LVLGAPGEGP LYALALGLLL VGLGFKAALA PFHFWTPDVY
210 220 230 240 250
QGSPTPVVLF MATSVKAAAF AALLRVAAPP EALALLVALS VVVGNLAALA
260 270 280 290 300
QKEAKRLLAY SSIAHAGYMA LALYTGNAQA LGFYLLTYVL ATGLAFAVLS
310 320 330 340 350
QISPDRVPLE ALRGLYRKDP LLGLAFLVAM LSLLGLPPLA GFWGKYLAFA
360 370 380 390 400
EAARAGAWGV LVLALVTSAV SAYYYLGLGL AVFARPEETP FRPGPPWARA
410 420
AVVAAGVLLL ALGLLPGLVL PALAAGG
Length:427
Mass (Da):44,436
Last modified:March 29, 2005 - v2
Checksum:i9AD943608E5C2759
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti81 – 811R → G in AAA97951. (PubMed:9020134)Curated
Sequence conflicti157 – 1571A → D in AAA97951. (PubMed:9020134)Curated
Sequence conflicti233 – 2331L → V in AAA97951. (PubMed:9020134)Curated
Sequence conflicti407 – 42721VLLLA…LAAGG → SSSSPWGSSPASSSPPWPRG VKITP in AAA97951. (PubMed:9020134)CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52917 Genomic DNA. Translation: AAA97951.1.
AP008226 Genomic DNA. Translation: BAD69920.1.
PIRiT11911.
RefSeqiYP_143363.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD69920; BAD69920; BAD69920.
GeneIDi3169623.
KEGGittj:TTHA0097.
PATRICi23955125. VBITheThe93045_0095.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52917 Genomic DNA. Translation: AAA97951.1 .
AP008226 Genomic DNA. Translation: BAD69920.1 .
PIRi T11911.
RefSeqi YP_143363.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4HE8 X-ray 3.30 I/N 1-427 [» ]
4HEA X-ray 3.30 N/V 1-427 [» ]
ProteinModelPortali Q56229.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-59272N.
STRINGi 300852.TTHA0097.

Protein family/group databases

TCDBi 3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD69920 ; BAD69920 ; BAD69920 .
GeneIDi 3169623.
KEGGi ttj:TTHA0097.
PATRICi 23955125. VBITheThe93045_0095.

Phylogenomic databases

eggNOGi COG1007.
HOGENOMi HOG000100794.
KOi K00343.
OMAi RIIIALY.
OrthoDBi EOG64JFNZ.
PhylomeDBi Q56229.

Enzyme and pathway databases

BioCyci TTHE300852:GH8R-101-MONOMER.

Family and domain databases

HAMAPi MF_00445. NDH1_NuoN_1.
InterProi IPR010096. NADH-Q_OxRdtase_suN/2.
IPR001750. NADH_UbQ/plastoQ_OxRdtase.
[Graphical view ]
Pfami PF00361. Oxidored_q1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit."
    Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T.
    J. Biol. Chem. 272:4201-4211(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.

Entry informationi

Entry nameiNQO14_THET8
AccessioniPrimary (citable) accession number: Q56229
Secondary accession number(s): Q5SM46
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: March 29, 2005
Last modified: November 26, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3