Reviewed, UniProtKB/Swiss-Prot Q56229 (NQO14_THET8)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit 14
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I chain 14
    NDH-1 subunit 14

Gene names

Name:	nqo14
Ordered Locus Names:	TTHA0097

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Complete proteome] [HAMAP]

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Subunit structure	NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the complex I subunit 2 family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 427	427	NADH-quinone oxidoreductase subunit 14		PRO_0000117687
Regions
	Transmembrane	1 – 21	21	Potential
	Transmembrane	30 – 50	21	Potential
	Transmembrane	57 – 77	21	Potential
	Transmembrane	79 – 99	21	Potential
	Transmembrane	104 – 124	21	Potential
	Transmembrane	137 – 157	21	Potential
	Transmembrane	172 – 192	21	Potential
	Transmembrane	204 – 224	21	Potential
	Transmembrane	230 – 250	21	Potential
	Transmembrane	257 – 277	21	Potential
	Transmembrane	280 – 300	21	Potential
	Transmembrane	322 – 342	21	Potential
	Transmembrane	360 – 380	21	Potential
	Transmembrane	400 – 420	21	Potential
Experimental info
	Sequence conflict	81	1	R → G in AAA97951. Ref.1
	Sequence conflict	157	1	A → D in AAA97951. Ref.1
	Sequence conflict	233	1	L → V in AAA97951. Ref.1
	Sequence conflict	407 – 427	21	VLLLA…LAAGG → SSSSPWGSSPASSSPPWPRG VKITP in AAA97951. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q56229-1 [UniParc]. Last modified March 29, 2005. Version 2. Checksum: 9AD943608E5C2759	FASTA	427	44,436
	10 20 30 40 50 60 MTLAILAVFS VALTLLGFVL PPQGVKRATL LGLALALASL LLTWGKPFAF GPYAVDGVSQ 70 80 90 100 110 120 VFTLLALLGA LWTVGLVRSG RFEFYLLVLY AALGMHLLAS TRHLLLMLVA LEALSLPLYA 130 140 150 160 170 180 LATWRRGQGL EAALKYFLLG ALAAAFFLYG AALFYGATGS LVLGAPGEGP LYALALGLLL 190 200 210 220 230 240 VGLGFKAALA PFHFWTPDVY QGSPTPVVLF MATSVKAAAF AALLRVAAPP EALALLVALS 250 260 270 280 290 300 VVVGNLAALA QKEAKRLLAY SSIAHAGYMA LALYTGNAQA LGFYLLTYVL ATGLAFAVLS 310 320 330 340 350 360 QISPDRVPLE ALRGLYRKDP LLGLAFLVAM LSLLGLPPLA GFWGKYLAFA EAARAGAWGV 370 380 390 400 410 420 LVLALVTSAV SAYYYLGLGL AVFARPEETP FRPGPPWARA AVVAAGVLLL ALGLLPGLVL PALAAGG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit." Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T. J. Biol. Chem. 272:4201-4211(1997) [PubMed: 9020134] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U52917 Genomic DNA. Translation: AAA97951.1. AP008226 Genomic DNA. Translation: BAD69920.1.
PIR	T11911.
RefSeq	YP_143363.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q56229.
Protein family/group databases
TCDB	3.D.1.3.1. H+ or Na+-translocating NADH dehydrogenase (NDH) family.
Genome annotation databases
GeneID	3169623.
GenomeReviews	Gene locus TTHA0097 in contig AP008226_GR.
KEGG	ttj:TTHA0097.
NMPDR	fig|300852.3.peg.128.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1007.
HOGENOM	HBG747830.
OMA	SIGHIGF.
Enzyme and pathway databases
BioCyc	TTHE300852:TTHA0097-MONOMER.
Family and domain databases
HAMAP	MF_00445. NDH1_NuoN_1. [Tree]
InterPro	IPR010096. NADH-Q_OxRdtase_chainN. IPR001750. NADH_UbQ/plastoQ_OxRdtase. [Graphical view]
Pfam	PF00361. Oxidored_q1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01770. NDH_I_N. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NQO14_THET8

Accession

Primary (citable) accession number: Q56229
Secondary accession number(s): Q5SM46

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	March 29, 2005
Last modified:	February 9, 2010
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)