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Q56229 (NQO14_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH-quinone oxidoreductase subunit 14

EC=1.6.99.5
Alternative name(s):
NADH dehydrogenase I chain 14
NDH-1 subunit 14
Gene names
Name:nqo14
Ordered Locus Names:TTHA0097
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length427 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. HAMAP-Rule MF_00445

Catalytic activity

NADH + quinone = NAD+ + quinol. HAMAP-Rule MF_00445

Subunit structure

NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.

Subcellular location

Cell inner membrane; Multi-pass membrane protein HAMAP-Rule MF_00445.

Sequence similarities

Belongs to the complex I subunit 2 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 427427NADH-quinone oxidoreductase subunit 14 HAMAP-Rule MF_00445
PRO_0000117687

Regions

Transmembrane1 – 2121Helical; Potential
Transmembrane30 – 5021Helical; Potential
Transmembrane57 – 7721Helical; Potential
Transmembrane79 – 9921Helical; Potential
Transmembrane104 – 12421Helical; Potential
Transmembrane137 – 15721Helical; Potential
Transmembrane172 – 19221Helical; Potential
Transmembrane204 – 22421Helical; Potential
Transmembrane230 – 25021Helical; Potential
Transmembrane257 – 27721Helical; Potential
Transmembrane280 – 30021Helical; Potential
Transmembrane322 – 34221Helical; Potential
Transmembrane360 – 38021Helical; Potential
Transmembrane400 – 42021Helical; Potential

Experimental info

Sequence conflict811R → G in AAA97951. Ref.1
Sequence conflict1571A → D in AAA97951. Ref.1
Sequence conflict2331L → V in AAA97951. Ref.1
Sequence conflict407 – 42721VLLLA…LAAGG → SSSSPWGSSPASSSPPWPRG VKITP in AAA97951. Ref.1

Secondary structure

.................................................. 427
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q56229 [UniParc].

Last modified March 29, 2005. Version 2.
Checksum: 9AD943608E5C2759

FASTA42744,436
        10         20         30         40         50         60 
MTLAILAVFS VALTLLGFVL PPQGVKRATL LGLALALASL LLTWGKPFAF GPYAVDGVSQ 

        70         80         90        100        110        120 
VFTLLALLGA LWTVGLVRSG RFEFYLLVLY AALGMHLLAS TRHLLLMLVA LEALSLPLYA 

       130        140        150        160        170        180 
LATWRRGQGL EAALKYFLLG ALAAAFFLYG AALFYGATGS LVLGAPGEGP LYALALGLLL 

       190        200        210        220        230        240 
VGLGFKAALA PFHFWTPDVY QGSPTPVVLF MATSVKAAAF AALLRVAAPP EALALLVALS 

       250        260        270        280        290        300 
VVVGNLAALA QKEAKRLLAY SSIAHAGYMA LALYTGNAQA LGFYLLTYVL ATGLAFAVLS 

       310        320        330        340        350        360 
QISPDRVPLE ALRGLYRKDP LLGLAFLVAM LSLLGLPPLA GFWGKYLAFA EAARAGAWGV 

       370        380        390        400        410        420 
LVLALVTSAV SAYYYLGLGL AVFARPEETP FRPGPPWARA AVVAAGVLLL ALGLLPGLVL 


PALAAGG 

« Hide

References

« Hide 'large scale' references
[1]"The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit."
Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T.
J. Biol. Chem. 272:4201-4211(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U52917 Genomic DNA. Translation: AAA97951.1.
AP008226 Genomic DNA. Translation: BAD69920.1.
PIRT11911.
RefSeqYP_143363.1. NC_006461.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4HE8X-ray3.30I/N1-427[»]
4HEAX-ray3.30N/V1-427[»]
ProteinModelPortalQ56229.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-59272N.
STRING300852.TTHA0097.

Protein family/group databases

TCDB3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD69920; BAD69920; BAD69920.
GeneID3169623.
KEGGttj:TTHA0097.
PATRIC23955125. VBITheThe93045_0095.

Phylogenomic databases

eggNOGCOG1007.
HOGENOMHOG000100794.
KOK00343.
OMARIIIALY.
OrthoDBEOG64JFNZ.
PhylomeDBQ56229.

Enzyme and pathway databases

BioCycTTHE300852:GH8R-101-MONOMER.

Family and domain databases

HAMAPMF_00445. NDH1_NuoN_1.
InterProIPR010096. NADH-Q_OxRdtase_suN/2.
IPR001750. NADH_UbQ/plastoQ_OxRdtase.
[Graphical view]
PfamPF00361. Oxidored_q1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNQO14_THET8
AccessionPrimary (citable) accession number: Q56229
Secondary accession number(s): Q5SM46
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: March 29, 2005
Last modified: July 9, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references