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Q56229

- NQO14_THET8

UniProt

Q56229 - NQO14_THET8

Protein

NADH-quinone oxidoreductase subunit 14

Gene

nqo14

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 2 (29 Mar 2005)
      Previous versions | rss
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    Functioni

    NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.

    Catalytic activityi

    NADH + quinone = NAD+ + quinol.UniRule annotation

    GO - Molecular functioni

    1. NADH dehydrogenase (ubiquinone) activity Source: InterPro
    2. quinone binding Source: UniProtKB-KW

    GO - Biological processi

    1. ATP synthesis coupled electron transport Source: InterPro
    2. transport Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Transport

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciTTHE300852:GH8R-101-MONOMER.

    Protein family/group databases

    TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH-quinone oxidoreductase subunit 14 (EC:1.6.99.5UniRule annotation)
    Alternative name(s):
    NADH dehydrogenase I chain 14
    NDH-1 subunit 14
    Gene namesi
    Name:nqo14
    Ordered Locus Names:TTHA0097
    OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
    Taxonomic identifieri300852 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
    ProteomesiUP000000532: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 427427NADH-quinone oxidoreductase subunit 14PRO_0000117687Add
    BLAST

    Keywords - PTMi

    Quinone

    Interactioni

    Subunit structurei

    NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.

    Protein-protein interaction databases

    DIPiDIP-59272N.
    STRINGi300852.TTHA0097.

    Structurei

    Secondary structure

    1
    427
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi2 – 1615
    Turni17 – 193
    Helixi22 – 4221
    Turni43 – 453
    Beta strandi51 – 566
    Helixi57 – 7519
    Helixi83 – 9917
    Helixi104 – 12118
    Helixi127 – 15731
    Helixi170 – 18617
    Helixi195 – 2028
    Helixi205 – 22521
    Helixi230 – 24920
    Helixi254 – 26916
    Helixi270 – 2734
    Turni274 – 2763
    Helixi279 – 30224
    Helixi309 – 3124
    Helixi315 – 3184
    Helixi320 – 33415
    Helixi340 – 35516
    Helixi358 – 37013
    Helixi372 – 3798
    Helixi381 – 3833
    Helixi396 – 41419
    Turni416 – 4194
    Helixi420 – 4223
    Beta strandi423 – 4253

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4HE8X-ray3.30I/N1-427[»]
    4HEAX-ray3.30N/V1-427[»]
    ProteinModelPortaliQ56229.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1 – 2121HelicalUniRule annotationAdd
    BLAST
    Transmembranei30 – 5021HelicalUniRule annotationAdd
    BLAST
    Transmembranei57 – 7721HelicalUniRule annotationAdd
    BLAST
    Transmembranei79 – 9921HelicalUniRule annotationAdd
    BLAST
    Transmembranei104 – 12421HelicalUniRule annotationAdd
    BLAST
    Transmembranei137 – 15721HelicalUniRule annotationAdd
    BLAST
    Transmembranei172 – 19221HelicalUniRule annotationAdd
    BLAST
    Transmembranei204 – 22421HelicalUniRule annotationAdd
    BLAST
    Transmembranei230 – 25021HelicalUniRule annotationAdd
    BLAST
    Transmembranei257 – 27721HelicalUniRule annotationAdd
    BLAST
    Transmembranei280 – 30021HelicalUniRule annotationAdd
    BLAST
    Transmembranei322 – 34221HelicalUniRule annotationAdd
    BLAST
    Transmembranei360 – 38021HelicalUniRule annotationAdd
    BLAST
    Transmembranei400 – 42021HelicalUniRule annotationAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the complex I subunit 2 family.UniRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1007.
    HOGENOMiHOG000100794.
    KOiK00343.
    OMAiRIIIALY.
    OrthoDBiEOG64JFNZ.
    PhylomeDBiQ56229.

    Family and domain databases

    HAMAPiMF_00445. NDH1_NuoN_1.
    InterProiIPR010096. NADH-Q_OxRdtase_suN/2.
    IPR001750. NADH_UbQ/plastoQ_OxRdtase.
    [Graphical view]
    PfamiPF00361. Oxidored_q1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q56229-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTLAILAVFS VALTLLGFVL PPQGVKRATL LGLALALASL LLTWGKPFAF    50
    GPYAVDGVSQ VFTLLALLGA LWTVGLVRSG RFEFYLLVLY AALGMHLLAS 100
    TRHLLLMLVA LEALSLPLYA LATWRRGQGL EAALKYFLLG ALAAAFFLYG 150
    AALFYGATGS LVLGAPGEGP LYALALGLLL VGLGFKAALA PFHFWTPDVY 200
    QGSPTPVVLF MATSVKAAAF AALLRVAAPP EALALLVALS VVVGNLAALA 250
    QKEAKRLLAY SSIAHAGYMA LALYTGNAQA LGFYLLTYVL ATGLAFAVLS 300
    QISPDRVPLE ALRGLYRKDP LLGLAFLVAM LSLLGLPPLA GFWGKYLAFA 350
    EAARAGAWGV LVLALVTSAV SAYYYLGLGL AVFARPEETP FRPGPPWARA 400
    AVVAAGVLLL ALGLLPGLVL PALAAGG 427
    Length:427
    Mass (Da):44,436
    Last modified:March 29, 2005 - v2
    Checksum:i9AD943608E5C2759
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti81 – 811R → G in AAA97951. (PubMed:9020134)Curated
    Sequence conflicti157 – 1571A → D in AAA97951. (PubMed:9020134)Curated
    Sequence conflicti233 – 2331L → V in AAA97951. (PubMed:9020134)Curated
    Sequence conflicti407 – 42721VLLLA…LAAGG → SSSSPWGSSPASSSPPWPRG VKITP in AAA97951. (PubMed:9020134)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U52917 Genomic DNA. Translation: AAA97951.1.
    AP008226 Genomic DNA. Translation: BAD69920.1.
    PIRiT11911.
    RefSeqiYP_143363.1. NC_006461.1.

    Genome annotation databases

    EnsemblBacteriaiBAD69920; BAD69920; BAD69920.
    GeneIDi3169623.
    KEGGittj:TTHA0097.
    PATRICi23955125. VBITheThe93045_0095.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U52917 Genomic DNA. Translation: AAA97951.1 .
    AP008226 Genomic DNA. Translation: BAD69920.1 .
    PIRi T11911.
    RefSeqi YP_143363.1. NC_006461.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4HE8 X-ray 3.30 I/N 1-427 [» ]
    4HEA X-ray 3.30 N/V 1-427 [» ]
    ProteinModelPortali Q56229.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-59272N.
    STRINGi 300852.TTHA0097.

    Protein family/group databases

    TCDBi 3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD69920 ; BAD69920 ; BAD69920 .
    GeneIDi 3169623.
    KEGGi ttj:TTHA0097.
    PATRICi 23955125. VBITheThe93045_0095.

    Phylogenomic databases

    eggNOGi COG1007.
    HOGENOMi HOG000100794.
    KOi K00343.
    OMAi RIIIALY.
    OrthoDBi EOG64JFNZ.
    PhylomeDBi Q56229.

    Enzyme and pathway databases

    BioCyci TTHE300852:GH8R-101-MONOMER.

    Family and domain databases

    HAMAPi MF_00445. NDH1_NuoN_1.
    InterProi IPR010096. NADH-Q_OxRdtase_suN/2.
    IPR001750. NADH_UbQ/plastoQ_OxRdtase.
    [Graphical view ]
    Pfami PF00361. Oxidored_q1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit."
      Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T.
      J. Biol. Chem. 272:4201-4211(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete genome sequence of Thermus thermophilus HB8."
      Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HB8 / ATCC 27634 / DSM 579.

    Entry informationi

    Entry nameiNQO14_THET8
    AccessioniPrimary (citable) accession number: Q56229
    Secondary accession number(s): Q5SM46
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: March 29, 2005
    Last modified: October 1, 2014
    This is version 97 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3