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Q56228

- NQO13_THET8

UniProt

Q56228 - NQO13_THET8

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Protein
NADH-quinone oxidoreductase subunit 13
Gene
nqo13, TTHA0096
Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.

Catalytic activityi

NADH + quinone = NAD+ + quinol.

GO - Molecular functioni

  1. NADH dehydrogenase (ubiquinone) activity Source: InterPro
  2. quinone binding Source: UniProtKB-KW

GO - Biological processi

  1. ATP synthesis coupled electron transport Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-100-MONOMER.

Protein family/group databases

TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit 13 (EC:1.6.99.5)
Alternative name(s):
NADH dehydrogenase I chain 13
NDH-1 subunit 13
Gene namesi
Name:nqo13
Ordered Locus Names:TTHA0096
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
ProteomesiUP000000532: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1 – 2121Helical; Reviewed prediction
Add
BLAST
Transmembranei23 – 4321Helical; Reviewed prediction
Add
BLAST
Transmembranei47 – 6721Helical; Reviewed prediction
Add
BLAST
Transmembranei69 – 8921Helical; Reviewed prediction
Add
BLAST
Transmembranei91 – 11121Helical; Reviewed prediction
Add
BLAST
Transmembranei115 – 13521Helical; Reviewed prediction
Add
BLAST
Transmembranei144 – 16421Helical; Reviewed prediction
Add
BLAST
Transmembranei190 – 21021Helical; Reviewed prediction
Add
BLAST
Transmembranei258 – 27821Helical; Reviewed prediction
Add
BLAST
Transmembranei284 – 30421Helical; Reviewed prediction
Add
BLAST
Transmembranei308 – 32821Helical; Reviewed prediction
Add
BLAST
Transmembranei354 – 37421Helical; Reviewed prediction
Add
BLAST
Transmembranei390 – 41021Helical; Reviewed prediction
Add
BLAST
Transmembranei430 – 45021Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 469469NADH-quinone oxidoreductase subunit 13
PRO_0000118038Add
BLAST

Keywords - PTMi

Quinone

Interactioni

Subunit structurei

NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.

Protein-protein interaction databases

DIPiDIP-59271N.
STRINGi300852.TTHA0096.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 1716
Helixi23 – 4018
Beta strandi45 – 473
Beta strandi51 – 566
Turni57 – 593
Beta strandi62 – 654
Helixi69 – 8820
Helixi96 – 11116
Helixi115 – 12410
Helixi126 – 1349
Helixi141 – 16828
Turni169 – 1713
Beta strandi173 – 1764
Helixi177 – 1826
Turni187 – 1893
Helixi190 – 20314
Turni204 – 2063
Helixi208 – 2103
Helixi214 – 2207
Helixi226 – 2338
Helixi237 – 2437
Helixi245 – 2484
Helixi250 – 27425
Helixi275 – 2773
Helixi281 – 30121
Helixi305 – 33632
Beta strandi340 – 3445
Turni347 – 3504
Helixi354 – 36512
Helixi373 – 38715
Helixi389 – 3968
Helixi399 – 41315
Beta strandi416 – 4183
Helixi427 – 44519
Turni447 – 4537
Helixi454 – 46310

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HE8X-ray3.30G/M1-469[»]
4HEAX-ray3.30M/U1-469[»]
ProteinModelPortaliQ56228.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1008.
HOGENOMiHOG000100683.
KOiK00342.
OMAiFFMVGAT.
OrthoDBiEOG647TZ6.
PhylomeDBiQ56228.

Family and domain databases

InterProiIPR010227. NADH_Q_OxRdtase_chainM/4.
IPR001750. NADH_UbQ/plastoQ_OxRdtase.
IPR003918. NADH_UbQ_OxRdtase.
[Graphical view]
PfamiPF00361. Oxidored_q1. 1 hit.
[Graphical view]
PRINTSiPR01437. NUOXDRDTASE4.
TIGRFAMsiTIGR01972. NDH_I_M. 1 hit.

Sequencei

Sequence statusi: Complete.

Q56228-1 [UniParc]FASTAAdd to Basket

« Hide

MVVLAVLLPV VFGALLLLGL PRALGVLGAG LSFLLNLYLF LTHPGGVAHA    50
FQAPLLPGAG VYWAFGLDGL SALFFLTIAL TVFLGALVAR VEGRFLGLAL 100
LMEGLLLGLF AARDLLVFYV FFEAALIPAL LMLYLYGGEG RTRALYTFVL 150
FTLVGSLPML AAVLGARLLS GSPTFLLEDL LAHPLQEEAA FWVFLGFALA 200
FAIKTPLFPL HAWLPPFHQE NHPSGLADAL GTLYKVGVFA FFRFAIPLAP 250
EGFAQAQGLL LFLAALSALY GAWVAFAAKD FKTLLAYAGL SHMGVAALGV 300
FSGTPEGAMG GLYLLAASGV YTGGLFLLAG RLYERTGTLE IGRYRGLAQS 350
APGLAALALI LFLAMVGLPG LSGFPGEFLT LLGAYKASPW LAALAFLSVI 400
ASAAYALTAF QKTFWEEGGS GVKDLAGAEW GFALLSVLAL LLMGVFPGYF 450
ARGLHPLAEA FAKLLGGGA 469
Length:469
Mass (Da):49,219
Last modified:March 29, 2005 - v2
Checksum:i5FD2D14713790224
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti166 – 1661A → D in AAA97950. 1 Publication
Sequence conflicti202 – 2021A → D in AAA97950. 1 Publication
Sequence conflicti240 – 2401A → D in AAA97950. 1 Publication
Sequence conflicti245 – 2451A → D in AAA97950. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U52917 Genomic DNA. Translation: AAA97950.1.
AP008226 Genomic DNA. Translation: BAD69919.1.
PIRiT11910.
RefSeqiWP_011227706.1. NC_006461.1.
YP_143362.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD69919; BAD69919; BAD69919.
GeneIDi3169618.
KEGGittj:TTHA0096.
PATRICi23955123. VBITheThe93045_0094.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U52917 Genomic DNA. Translation: AAA97950.1 .
AP008226 Genomic DNA. Translation: BAD69919.1 .
PIRi T11910.
RefSeqi WP_011227706.1. NC_006461.1.
YP_143362.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4HE8 X-ray 3.30 G/M 1-469 [» ]
4HEA X-ray 3.30 M/U 1-469 [» ]
ProteinModelPortali Q56228.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-59271N.
STRINGi 300852.TTHA0096.

Protein family/group databases

TCDBi 3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD69919 ; BAD69919 ; BAD69919 .
GeneIDi 3169618.
KEGGi ttj:TTHA0096.
PATRICi 23955123. VBITheThe93045_0094.

Phylogenomic databases

eggNOGi COG1008.
HOGENOMi HOG000100683.
KOi K00342.
OMAi FFMVGAT.
OrthoDBi EOG647TZ6.
PhylomeDBi Q56228.

Enzyme and pathway databases

BioCyci TTHE300852:GH8R-100-MONOMER.

Family and domain databases

InterProi IPR010227. NADH_Q_OxRdtase_chainM/4.
IPR001750. NADH_UbQ/plastoQ_OxRdtase.
IPR003918. NADH_UbQ_OxRdtase.
[Graphical view ]
Pfami PF00361. Oxidored_q1. 1 hit.
[Graphical view ]
PRINTSi PR01437. NUOXDRDTASE4.
TIGRFAMsi TIGR01972. NDH_I_M. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit."
    Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T.
    J. Biol. Chem. 272:4201-4211(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.

Entry informationi

Entry nameiNQO13_THET8
AccessioniPrimary (citable) accession number: Q56228
Secondary accession number(s): Q5SM47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: March 29, 2005
Last modified: September 3, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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