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Q56228 (NQO13_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
NADH-quinone oxidoreductase subunit 13
EC=1.6.99.5
Alternative name(s):
NADH dehydrogenase I chain 13
NDH-1 subunit 13
Gene names
Name:nqo13
Ordered Locus Names:TTHA0096
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length469 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.

Catalytic activity

NADH + quinone = NAD+ + quinol.

Subunit structure

NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.

Subcellular location

Cell inner membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the complex I subunit 4 family.

Ontologies

Keywords
   Cellular componentCell inner membrane
Cell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   LigandNAD
   Molecular functionOxidoreductase
   PTMQuinone
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processATP synthesis coupled electron transport

Inferred from electronic annotation. Source: InterPro

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionNADH dehydrogenase (ubiquinone) activity

Inferred from electronic annotation. Source: InterPro

quinone binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 469469NADH-quinone oxidoreductase subunit 13
PRO_0000118038

Regions

Transmembrane1 – 2121Helical; Potential
Transmembrane23 – 4321Helical; Potential
Transmembrane47 – 6721Helical; Potential
Transmembrane69 – 8921Helical; Potential
Transmembrane91 – 11121Helical; Potential
Transmembrane115 – 13521Helical; Potential
Transmembrane144 – 16421Helical; Potential
Transmembrane190 – 21021Helical; Potential
Transmembrane258 – 27821Helical; Potential
Transmembrane284 – 30421Helical; Potential
Transmembrane308 – 32821Helical; Potential
Transmembrane354 – 37421Helical; Potential
Transmembrane390 – 41021Helical; Potential
Transmembrane430 – 45021Helical; Potential

Experimental info

Sequence conflict1661A → D in AAA97950. Ref.1
Sequence conflict2021A → D in AAA97950. Ref.1
Sequence conflict2401A → D in AAA97950. Ref.1
Sequence conflict2451A → D in AAA97950. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q56228 [UniParc].

Last modified March 29, 2005. Version 2.
Checksum: 5FD2D14713790224

FASTA46949,219
        10         20         30         40         50         60 
MVVLAVLLPV VFGALLLLGL PRALGVLGAG LSFLLNLYLF LTHPGGVAHA FQAPLLPGAG 

        70         80         90        100        110        120 
VYWAFGLDGL SALFFLTIAL TVFLGALVAR VEGRFLGLAL LMEGLLLGLF AARDLLVFYV 

       130        140        150        160        170        180 
FFEAALIPAL LMLYLYGGEG RTRALYTFVL FTLVGSLPML AAVLGARLLS GSPTFLLEDL 

       190        200        210        220        230        240 
LAHPLQEEAA FWVFLGFALA FAIKTPLFPL HAWLPPFHQE NHPSGLADAL GTLYKVGVFA 

       250        260        270        280        290        300 
FFRFAIPLAP EGFAQAQGLL LFLAALSALY GAWVAFAAKD FKTLLAYAGL SHMGVAALGV 

       310        320        330        340        350        360 
FSGTPEGAMG GLYLLAASGV YTGGLFLLAG RLYERTGTLE IGRYRGLAQS APGLAALALI 

       370        380        390        400        410        420 
LFLAMVGLPG LSGFPGEFLT LLGAYKASPW LAALAFLSVI ASAAYALTAF QKTFWEEGGS 

       430        440        450        460 
GVKDLAGAEW GFALLSVLAL LLMGVFPGYF ARGLHPLAEA FAKLLGGGA

« Hide

References

« Hide 'large scale' references
[1]"The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit."
Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T.
J. Biol. Chem. 272:4201-4211(1997) [PubMed: 9020134] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U52917 Genomic DNA. Translation: AAA97950.1.
AP008226 Genomic DNA. Translation: BAD69919.1.
PIRT11910.
RefSeqYP_143362.1. NC_006461.1.

3D structure databases

ProteinModelPortalQ56228.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ56228.

Protein family/group databases

TCDB3.D.1.3.1. H+ or Na+-translocating NADH dehydrogenase (NDH) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3169618.
GenomeReviewsGene locus TTHA0096 in contig AP008226_GR.
KEGGttj:TTHA0096.
NMPDRfig|300852.3.peg.127.
PATRIC23955123. VBITheThe93045_0094.

Phylogenomic databases

eggNOGCOG1008.
HOGENOMHBG643593.
OMALYEASTN.
PhylomeDBQ56228.
ProtClustDBCLSK445095.

Enzyme and pathway databases

BioCycTTHE300852:TTHA0096-MONOMER.

Family and domain databases

InterProIPR010227. NADH_Q_OxRdtase_chainM/4.
IPR001750. NADH_UbQ/plastoQ_OxRdtase.
IPR003918. NADH_UbQ_OxRdtase.
[Graphical view]
KOK00342.
PfamPF00361. Oxidored_q1. 1 hit.
[Graphical view]
PRINTSPR01437. NUOXDRDTASE4.
TIGRFAMsTIGR01972. NDH_I_M. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNQO13_THET8
AccessionPrimary (citable) accession number: Q56228
Secondary accession number(s): Q5SM47
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: March 29, 2005
Last modified: January 25, 2012
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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