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Q56228

- NQO13_THET8

UniProt

Q56228 - NQO13_THET8

Protein

NADH-quinone oxidoreductase subunit 13

Gene

nqo13

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 2 (29 Mar 2005)
      Previous versions | rss
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    Functioni

    NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.

    Catalytic activityi

    NADH + quinone = NAD+ + quinol.

    GO - Molecular functioni

    1. NADH dehydrogenase (ubiquinone) activity Source: InterPro
    2. quinone binding Source: UniProtKB-KW

    GO - Biological processi

    1. ATP synthesis coupled electron transport Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciTTHE300852:GH8R-100-MONOMER.

    Protein family/group databases

    TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH-quinone oxidoreductase subunit 13 (EC:1.6.99.5)
    Alternative name(s):
    NADH dehydrogenase I chain 13
    NDH-1 subunit 13
    Gene namesi
    Name:nqo13
    Ordered Locus Names:TTHA0096
    OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
    Taxonomic identifieri300852 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
    ProteomesiUP000000532: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 469469NADH-quinone oxidoreductase subunit 13PRO_0000118038Add
    BLAST

    Keywords - PTMi

    Quinone

    Interactioni

    Subunit structurei

    NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.

    Protein-protein interaction databases

    DIPiDIP-59271N.
    STRINGi300852.TTHA0096.

    Structurei

    Secondary structure

    1
    469
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi2 – 1716
    Helixi23 – 4018
    Beta strandi45 – 473
    Beta strandi51 – 566
    Turni57 – 593
    Beta strandi62 – 654
    Helixi69 – 8820
    Helixi96 – 11116
    Helixi115 – 12410
    Helixi126 – 1349
    Helixi141 – 16828
    Turni169 – 1713
    Beta strandi173 – 1764
    Helixi177 – 1826
    Turni187 – 1893
    Helixi190 – 20314
    Turni204 – 2063
    Helixi208 – 2103
    Helixi214 – 2207
    Helixi226 – 2338
    Helixi237 – 2437
    Helixi245 – 2484
    Helixi250 – 27425
    Helixi275 – 2773
    Helixi281 – 30121
    Helixi305 – 33632
    Beta strandi340 – 3445
    Turni347 – 3504
    Helixi354 – 36512
    Helixi373 – 38715
    Helixi389 – 3968
    Helixi399 – 41315
    Beta strandi416 – 4183
    Helixi427 – 44519
    Turni447 – 4537
    Helixi454 – 46310

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4HE8X-ray3.30G/M1-469[»]
    4HEAX-ray3.30M/U1-469[»]
    ProteinModelPortaliQ56228.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1 – 2121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei23 – 4321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei47 – 6721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei69 – 8921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei91 – 11121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei115 – 13521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei144 – 16421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei190 – 21021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei258 – 27821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei284 – 30421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei308 – 32821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei354 – 37421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei390 – 41021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei430 – 45021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the complex I subunit 4 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1008.
    HOGENOMiHOG000100683.
    KOiK00342.
    OMAiFFMVGAT.
    OrthoDBiEOG647TZ6.
    PhylomeDBiQ56228.

    Family and domain databases

    InterProiIPR010227. NADH_Q_OxRdtase_chainM/4.
    IPR001750. NADH_UbQ/plastoQ_OxRdtase.
    IPR003918. NADH_UbQ_OxRdtase.
    [Graphical view]
    PfamiPF00361. Oxidored_q1. 1 hit.
    [Graphical view]
    PRINTSiPR01437. NUOXDRDTASE4.
    TIGRFAMsiTIGR01972. NDH_I_M. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q56228-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVVLAVLLPV VFGALLLLGL PRALGVLGAG LSFLLNLYLF LTHPGGVAHA    50
    FQAPLLPGAG VYWAFGLDGL SALFFLTIAL TVFLGALVAR VEGRFLGLAL 100
    LMEGLLLGLF AARDLLVFYV FFEAALIPAL LMLYLYGGEG RTRALYTFVL 150
    FTLVGSLPML AAVLGARLLS GSPTFLLEDL LAHPLQEEAA FWVFLGFALA 200
    FAIKTPLFPL HAWLPPFHQE NHPSGLADAL GTLYKVGVFA FFRFAIPLAP 250
    EGFAQAQGLL LFLAALSALY GAWVAFAAKD FKTLLAYAGL SHMGVAALGV 300
    FSGTPEGAMG GLYLLAASGV YTGGLFLLAG RLYERTGTLE IGRYRGLAQS 350
    APGLAALALI LFLAMVGLPG LSGFPGEFLT LLGAYKASPW LAALAFLSVI 400
    ASAAYALTAF QKTFWEEGGS GVKDLAGAEW GFALLSVLAL LLMGVFPGYF 450
    ARGLHPLAEA FAKLLGGGA 469
    Length:469
    Mass (Da):49,219
    Last modified:March 29, 2005 - v2
    Checksum:i5FD2D14713790224
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti166 – 1661A → D in AAA97950. (PubMed:9020134)Curated
    Sequence conflicti202 – 2021A → D in AAA97950. (PubMed:9020134)Curated
    Sequence conflicti240 – 2401A → D in AAA97950. (PubMed:9020134)Curated
    Sequence conflicti245 – 2451A → D in AAA97950. (PubMed:9020134)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U52917 Genomic DNA. Translation: AAA97950.1.
    AP008226 Genomic DNA. Translation: BAD69919.1.
    PIRiT11910.
    RefSeqiWP_011227706.1. NC_006461.1.
    YP_143362.1. NC_006461.1.

    Genome annotation databases

    EnsemblBacteriaiBAD69919; BAD69919; BAD69919.
    GeneIDi3169618.
    KEGGittj:TTHA0096.
    PATRICi23955123. VBITheThe93045_0094.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U52917 Genomic DNA. Translation: AAA97950.1 .
    AP008226 Genomic DNA. Translation: BAD69919.1 .
    PIRi T11910.
    RefSeqi WP_011227706.1. NC_006461.1.
    YP_143362.1. NC_006461.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4HE8 X-ray 3.30 G/M 1-469 [» ]
    4HEA X-ray 3.30 M/U 1-469 [» ]
    ProteinModelPortali Q56228.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-59271N.
    STRINGi 300852.TTHA0096.

    Protein family/group databases

    TCDBi 3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD69919 ; BAD69919 ; BAD69919 .
    GeneIDi 3169618.
    KEGGi ttj:TTHA0096.
    PATRICi 23955123. VBITheThe93045_0094.

    Phylogenomic databases

    eggNOGi COG1008.
    HOGENOMi HOG000100683.
    KOi K00342.
    OMAi FFMVGAT.
    OrthoDBi EOG647TZ6.
    PhylomeDBi Q56228.

    Enzyme and pathway databases

    BioCyci TTHE300852:GH8R-100-MONOMER.

    Family and domain databases

    InterProi IPR010227. NADH_Q_OxRdtase_chainM/4.
    IPR001750. NADH_UbQ/plastoQ_OxRdtase.
    IPR003918. NADH_UbQ_OxRdtase.
    [Graphical view ]
    Pfami PF00361. Oxidored_q1. 1 hit.
    [Graphical view ]
    PRINTSi PR01437. NUOXDRDTASE4.
    TIGRFAMsi TIGR01972. NDH_I_M. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit."
      Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T.
      J. Biol. Chem. 272:4201-4211(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete genome sequence of Thermus thermophilus HB8."
      Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HB8 / ATCC 27634 / DSM 579.

    Entry informationi

    Entry nameiNQO13_THET8
    AccessioniPrimary (citable) accession number: Q56228
    Secondary accession number(s): Q5SM47
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: March 29, 2005
    Last modified: October 1, 2014
    This is version 96 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3