Reviewed, UniProtKB/Swiss-Prot Q56228 (NQO13_THET8)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit 13
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I chain 13
    NDH-1 subunit 13

Gene names

Name:	nqo13
Ordered Locus Names:	TTHA0096

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Complete proteome] [HAMAP]

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Subunit structure	NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the complex I subunit 4 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	NADH-quinone oxidoreductase subunit 13		PRO_0000118038
Regions
	Transmembrane	1 – 21	21	Potential
	Transmembrane	23 – 43	21	Potential
	Transmembrane	47 – 67	21	Potential
	Transmembrane	69 – 89	21	Potential
	Transmembrane	91 – 111	21	Potential
	Transmembrane	115 – 135	21	Potential
	Transmembrane	144 – 164	21	Potential
	Transmembrane	190 – 210	21	Potential
	Transmembrane	258 – 278	21	Potential
	Transmembrane	284 – 304	21	Potential
	Transmembrane	308 – 328	21	Potential
	Transmembrane	354 – 374	21	Potential
	Transmembrane	390 – 410	21	Potential
	Transmembrane	430 – 450	21	Potential
Experimental info
	Sequence conflict	166	1	A → D in AAA97950. Ref.1
	Sequence conflict	202	1	A → D in AAA97950. Ref.1
	Sequence conflict	240	1	A → D in AAA97950. Ref.1
	Sequence conflict	245	1	A → D in AAA97950. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q56228-1 [UniParc]. Last modified March 29, 2005. Version 2. Checksum: 5FD2D14713790224	FASTA	469	49,219
	10 20 30 40 50 60 MVVLAVLLPV VFGALLLLGL PRALGVLGAG LSFLLNLYLF LTHPGGVAHA FQAPLLPGAG 70 80 90 100 110 120 VYWAFGLDGL SALFFLTIAL TVFLGALVAR VEGRFLGLAL LMEGLLLGLF AARDLLVFYV 130 140 150 160 170 180 FFEAALIPAL LMLYLYGGEG RTRALYTFVL FTLVGSLPML AAVLGARLLS GSPTFLLEDL 190 200 210 220 230 240 LAHPLQEEAA FWVFLGFALA FAIKTPLFPL HAWLPPFHQE NHPSGLADAL GTLYKVGVFA 250 260 270 280 290 300 FFRFAIPLAP EGFAQAQGLL LFLAALSALY GAWVAFAAKD FKTLLAYAGL SHMGVAALGV 310 320 330 340 350 360 FSGTPEGAMG GLYLLAASGV YTGGLFLLAG RLYERTGTLE IGRYRGLAQS APGLAALALI 370 380 390 400 410 420 LFLAMVGLPG LSGFPGEFLT LLGAYKASPW LAALAFLSVI ASAAYALTAF QKTFWEEGGS 430 440 450 460 GVKDLAGAEW GFALLSVLAL LLMGVFPGYF ARGLHPLAEA FAKLLGGGA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit." Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T. J. Biol. Chem. 272:4201-4211(1997) [PubMed: 9020134] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U52917 Genomic DNA. Translation: AAA97950.1. AP008226 Genomic DNA. Translation: BAD69919.1.
PIR	T11910.
RefSeq	YP_143362.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q56228.
Protein family/group databases
TCDB	3.D.1.3.1. H+ or Na+-translocating NADH dehydrogenase (NDH) family.
Genome annotation databases
GeneID	3169618.
GenomeReviews	Gene locus TTHA0096 in contig AP008226_GR.
KEGG	ttj:TTHA0096.
NMPDR	fig|300852.3.peg.127.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1008.
HOGENOM	HBG643593.
OMA	EVHLQIA.
Enzyme and pathway databases
BioCyc	TTHE300852:TTHA0096-MONOMER.
Family and domain databases
InterPro	IPR010227. NADH_Q_OxRdtase_chainM. IPR001750. NADH_UbQ/plastoQ_OxRdtase. IPR003918. NADH_UbQ_OxRdtase_chain4. [Graphical view]
Pfam	PF00361. Oxidored_q1. 1 hit. [Graphical view]
PRINTS	PR01437. NUOXDRDTASE4.
TIGRFAMs	TIGR01972. NDH_I_M. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NQO13_THET8

Accession

Primary (citable) accession number: Q56228
Secondary accession number(s): Q5SM47

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	March 29, 2005
Last modified:	February 9, 2010
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)