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Protein

NADH-quinone oxidoreductase subunit 12

Gene

nqo12

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Protein family/group databases

TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit 12 (EC:1.6.5.11)
Alternative name(s):
NADH dehydrogenase I chain 12
NDH-1 subunit 12
Gene namesi
Name:nqo12
Ordered Locus Names:TTHA0095
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei1 – 21HelicalSequence analysisAdd BLAST21
Transmembranei29 – 49HelicalSequence analysisAdd BLAST21
Transmembranei75 – 95HelicalSequence analysisAdd BLAST21
Transmembranei104 – 124HelicalSequence analysisAdd BLAST21
Transmembranei126 – 146HelicalSequence analysisAdd BLAST21
Transmembranei165 – 185HelicalSequence analysisAdd BLAST21
Transmembranei206 – 226HelicalSequence analysisAdd BLAST21
Transmembranei234 – 254HelicalSequence analysisAdd BLAST21
Transmembranei266 – 286HelicalSequence analysisAdd BLAST21
Transmembranei303 – 323HelicalSequence analysisAdd BLAST21
Transmembranei325 – 345HelicalSequence analysisAdd BLAST21
Transmembranei364 – 384HelicalSequence analysisAdd BLAST21
Transmembranei397 – 417HelicalSequence analysisAdd BLAST21
Transmembranei441 – 461HelicalSequence analysisAdd BLAST21
Transmembranei492 – 512HelicalSequence analysisAdd BLAST21
Transmembranei584 – 604HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001182121 – 606NADH-quinone oxidoreductase subunit 12Add BLAST606

Keywords - PTMi

Quinone

Interactioni

Subunit structurei

NDH-1 is composed of 15 different subunits, Nqo1 to Nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits Nqo7, Nqo8 and Nqo10 to Nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits Nqo1 to Nqo6, Nqo9 and Nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.

Protein-protein interaction databases

DIPiDIP-59270N.
STRINGi300852.TTHA0095.

Structurei

Secondary structure

1606
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 12Combined sources11
Helixi14 – 19Combined sources6
Helixi28 – 49Combined sources22
Beta strandi53 – 57Combined sources5
Beta strandi65 – 69Combined sources5
Helixi71 – 94Combined sources24
Helixi101 – 119Combined sources19
Helixi124 – 142Combined sources19
Helixi150 – 178Combined sources29
Beta strandi179 – 181Combined sources3
Helixi185 – 190Combined sources6
Beta strandi192 – 195Combined sources4
Helixi199 – 216Combined sources18
Helixi222 – 231Combined sources10
Helixi234 – 241Combined sources8
Helixi246 – 255Combined sources10
Helixi257 – 260Combined sources4
Helixi264 – 283Combined sources20
Turni284 – 287Combined sources4
Helixi291 – 311Combined sources21
Helixi315 – 342Combined sources28
Turni356 – 358Combined sources3
Helixi362 – 374Combined sources13
Beta strandi377 – 379Combined sources3
Helixi380 – 394Combined sources15
Beta strandi395 – 397Combined sources3
Helixi400 – 425Combined sources26
Beta strandi426 – 428Combined sources3
Helixi440 – 455Combined sources16
Helixi457 – 459Combined sources3
Helixi470 – 476Combined sources7
Helixi489 – 511Combined sources23
Helixi519 – 521Combined sources3
Helixi522 – 525Combined sources4
Turni526 – 530Combined sources5
Helixi531 – 533Combined sources3
Helixi535 – 538Combined sources4
Turni539 – 542Combined sources4
Helixi543 – 578Combined sources36
Helixi586 – 604Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HE8X-ray3.30F/L1-606[»]
4HEAX-ray3.30L/T1-606[»]
ProteinModelPortaliQ56227.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I subunit 5 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D65. Bacteria.
COG1009. LUCA.
HOGENOMiHOG000100570.
KOiK00341.
OMAiMLNNADY.
PhylomeDBiQ56227.

Family and domain databases

InterProiIPR003945. NADHpl_OxRdtase_5.
IPR018393. NADHpl_OxRdtase_5_subgr.
IPR001750. ND/Mrp_mem.
IPR001516. Proton_antipo_N.
[Graphical view]
PfamiPF00361. Proton_antipo_M. 1 hit.
PF00662. Proton_antipo_N. 1 hit.
[Graphical view]
PRINTSiPR01435. NPOXDRDTASE5.
TIGRFAMsiTIGR01974. NDH_I_L. 1 hit.

Sequencei

Sequence statusi: Complete.

Q56227-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALLGTILLP LLGFALLGLF GKRMREPLPG VLASGLVLAS FLLGAGLLLS
60 70 80 90 100
GGARFQAEWL PGIPFSLLLD NLSGFMLLIV TGVGFLIHVY AIGYMGGDPG
110 120 130 140 150
YSRFFAYFNL FIAMMLTLVL ADSYPVMFIG WEGVGLASFL LIGFWYKNPQ
160 170 180 190 200
YADSARKAFI VNRIGDLGFM LGMAILWALY GTLSISELKE AMEGPLKNPD
210 220 230 240 250
LLALAGLLLF LGAVGKSAQI PLMVWLPDAM AGPTPVSALI HAATMVTAGV
260 270 280 290 300
YLIARSSFLY SVLPDVSYAI AVVGLLTAAY GALSAFGQTD IKKIVAYSTI
310 320 330 340 350
SQLGYMFLAA GVGAYWVALF HVFTHAFFKA LLFLASGSVI HALGGEQDVR
360 370 380 390 400
KMGGLWKHLP QTRWHALIGA LALGGLPLLS GFWSKDAILA ATLTYPFGGV
410 420 430 440 450
GFYVGALLVA VLTAMYAMRW FVLVFLGEER GHHHPHEAPP VMLWPNHLLA
460 470 480 490 500
LGSVLAGYLA LPHPLPNVLE PFLKPALAEV EAHHLSLGAE WGLIALSAAV
510 520 530 540 550
ALLGLWAGFV FFQRKVFPAW YLAFEAASRE AFYVDRAYNA LIVNPLKALA
560 570 580 590 600
EALFYGDRGL LSGYFGLGGA ARSLGQGLAR LQTGYLRVYA LLFVLGALLL

LGVMRW
Length:606
Mass (Da):65,141
Last modified:November 1, 1997 - v1
Checksum:i47318E8F2665295C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52917 Genomic DNA. Translation: AAA97949.1.
AP008226 Genomic DNA. Translation: BAD69918.1.
PIRiT11909.
RefSeqiWP_011227705.1. NC_006461.1.
YP_143361.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD69918; BAD69918; BAD69918.
GeneIDi3169613.
KEGGittj:TTHA0095.
PATRICi23955121. VBITheThe93045_0093.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52917 Genomic DNA. Translation: AAA97949.1.
AP008226 Genomic DNA. Translation: BAD69918.1.
PIRiT11909.
RefSeqiWP_011227705.1. NC_006461.1.
YP_143361.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HE8X-ray3.30F/L1-606[»]
4HEAX-ray3.30L/T1-606[»]
ProteinModelPortaliQ56227.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59270N.
STRINGi300852.TTHA0095.

Protein family/group databases

TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD69918; BAD69918; BAD69918.
GeneIDi3169613.
KEGGittj:TTHA0095.
PATRICi23955121. VBITheThe93045_0093.

Phylogenomic databases

eggNOGiENOG4105D65. Bacteria.
COG1009. LUCA.
HOGENOMiHOG000100570.
KOiK00341.
OMAiMLNNADY.
PhylomeDBiQ56227.

Family and domain databases

InterProiIPR003945. NADHpl_OxRdtase_5.
IPR018393. NADHpl_OxRdtase_5_subgr.
IPR001750. ND/Mrp_mem.
IPR001516. Proton_antipo_N.
[Graphical view]
PfamiPF00361. Proton_antipo_M. 1 hit.
PF00662. Proton_antipo_N. 1 hit.
[Graphical view]
PRINTSiPR01435. NPOXDRDTASE5.
TIGRFAMsiTIGR01974. NDH_I_L. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNQO12_THET8
AccessioniPrimary (citable) accession number: Q56227
Secondary accession number(s): Q5SM48
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.