Reviewed, UniProtKB/Swiss-Prot Q56227 (NQO12_THET8)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit 12
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I chain 12
    NDH-1 subunit 12

Gene names

Name:	nqo12
Ordered Locus Names:	TTHA0095

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Complete proteome] [HAMAP]

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	606 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Subunit structure	NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the complex I subunit 5 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 606	606	NADH-quinone oxidoreductase subunit 12		PRO_0000118212
Regions
	Transmembrane	1 – 21	21	Potential
	Transmembrane	29 – 49	21	Potential
	Transmembrane	75 – 95	21	Potential
	Transmembrane	104 – 124	21	Potential
	Transmembrane	126 – 146	21	Potential
	Transmembrane	165 – 185	21	Potential
	Transmembrane	206 – 226	21	Potential
	Transmembrane	234 – 254	21	Potential
	Transmembrane	266 – 286	21	Potential
	Transmembrane	303 – 323	21	Potential
	Transmembrane	325 – 345	21	Potential
	Transmembrane	364 – 384	21	Potential
	Transmembrane	397 – 417	21	Potential
	Transmembrane	441 – 461	21	Potential
	Transmembrane	492 – 512	21	Potential
	Transmembrane	584 – 604	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q56227-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 47318E8F2665295C	FASTA	606	65,141
	10 20 30 40 50 60 MALLGTILLP LLGFALLGLF GKRMREPLPG VLASGLVLAS FLLGAGLLLS GGARFQAEWL 70 80 90 100 110 120 PGIPFSLLLD NLSGFMLLIV TGVGFLIHVY AIGYMGGDPG YSRFFAYFNL FIAMMLTLVL 130 140 150 160 170 180 ADSYPVMFIG WEGVGLASFL LIGFWYKNPQ YADSARKAFI VNRIGDLGFM LGMAILWALY 190 200 210 220 230 240 GTLSISELKE AMEGPLKNPD LLALAGLLLF LGAVGKSAQI PLMVWLPDAM AGPTPVSALI 250 260 270 280 290 300 HAATMVTAGV YLIARSSFLY SVLPDVSYAI AVVGLLTAAY GALSAFGQTD IKKIVAYSTI 310 320 330 340 350 360 SQLGYMFLAA GVGAYWVALF HVFTHAFFKA LLFLASGSVI HALGGEQDVR KMGGLWKHLP 370 380 390 400 410 420 QTRWHALIGA LALGGLPLLS GFWSKDAILA ATLTYPFGGV GFYVGALLVA VLTAMYAMRW 430 440 450 460 470 480 FVLVFLGEER GHHHPHEAPP VMLWPNHLLA LGSVLAGYLA LPHPLPNVLE PFLKPALAEV 490 500 510 520 530 540 EAHHLSLGAE WGLIALSAAV ALLGLWAGFV FFQRKVFPAW YLAFEAASRE AFYVDRAYNA 550 560 570 580 590 600 LIVNPLKALA EALFYGDRGL LSGYFGLGGA ARSLGQGLAR LQTGYLRVYA LLFVLGALLL LGVMRW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit." Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T. J. Biol. Chem. 272:4201-4211(1997) [PubMed: 9020134] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U52917 Genomic DNA. Translation: AAA97949.1. AP008226 Genomic DNA. Translation: BAD69918.1.
PIR	T11909.
RefSeq	YP_143361.1.
3D structure databases
ModBase	Search...
Protein family/group databases
TCDB	3.D.1.3.1. proton-translocating NADH dehydrogenase (NDH) family.
Genome annotation databases
GeneID	3169613.
GenomeReviews	Gene locus TTHA0095 in contig AP008226_GR.
KEGG	ttj:TTHA0095.
NMPDR	fig|300852.3.peg.126.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q56227.
OMA	Q56227. VLADSYP.
Enzyme and pathway databases
BioCyc	TTHE300852:TTHA0095-MON.
Family and domain databases
InterPro	IPR001750. NADH_UbQ/plastoQ_OxRdtase. IPR003916. NADH_UbQ_OxRdtase_chain5. IPR001516. NADH_UbQ_OxRdtase_chain5/L_N. IPR003945. NADHpl_OxRdtase_5. IPR018393. NADHpl_OxRdtase_5_sg. [Graphical view]
Pfam	PF00361. Oxidored_q1. 1 hit. PF00662. Oxidored_q1_N. 1 hit. [Graphical view]
PRINTS	PR01434. NADHDHGNASE5. PR01435. NPOXDRDTASE5.
TIGRFAMs	TIGR01974. NDH_I_L. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NQO12_THET8

Accession

Primary (citable) accession number: Q56227
Secondary accession number(s): Q5SM48

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)