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Q56227

- NQO12_THET8

UniProt

Q56227 - NQO12_THET8

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Protein

NADH-quinone oxidoreductase subunit 12

Gene

nqo12

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.

Catalytic activityi

NADH + quinone = NAD+ + quinol.

GO - Molecular functioni

  1. NADH dehydrogenase (ubiquinone) activity Source: InterPro
  2. quinone binding Source: UniProtKB-KW

GO - Biological processi

  1. ATP synthesis coupled electron transport Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-99-MONOMER.

Protein family/group databases

TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit 12 (EC:1.6.99.5)
Alternative name(s):
NADH dehydrogenase I chain 12
NDH-1 subunit 12
Gene namesi
Name:nqo12
Ordered Locus Names:TTHA0095
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
ProteomesiUP000000532: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1 – 2121HelicalSequence AnalysisAdd
BLAST
Transmembranei29 – 4921HelicalSequence AnalysisAdd
BLAST
Transmembranei75 – 9521HelicalSequence AnalysisAdd
BLAST
Transmembranei104 – 12421HelicalSequence AnalysisAdd
BLAST
Transmembranei126 – 14621HelicalSequence AnalysisAdd
BLAST
Transmembranei165 – 18521HelicalSequence AnalysisAdd
BLAST
Transmembranei206 – 22621HelicalSequence AnalysisAdd
BLAST
Transmembranei234 – 25421HelicalSequence AnalysisAdd
BLAST
Transmembranei266 – 28621HelicalSequence AnalysisAdd
BLAST
Transmembranei303 – 32321HelicalSequence AnalysisAdd
BLAST
Transmembranei325 – 34521HelicalSequence AnalysisAdd
BLAST
Transmembranei364 – 38421HelicalSequence AnalysisAdd
BLAST
Transmembranei397 – 41721HelicalSequence AnalysisAdd
BLAST
Transmembranei441 – 46121HelicalSequence AnalysisAdd
BLAST
Transmembranei492 – 51221HelicalSequence AnalysisAdd
BLAST
Transmembranei584 – 60421HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 606606NADH-quinone oxidoreductase subunit 12PRO_0000118212Add
BLAST

Keywords - PTMi

Quinone

Interactioni

Subunit structurei

NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.

Protein-protein interaction databases

DIPiDIP-59270N.
STRINGi300852.TTHA0095.

Structurei

Secondary structure

1
606
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 1211Combined sources
Helixi14 – 196Combined sources
Helixi28 – 4922Combined sources
Beta strandi53 – 575Combined sources
Beta strandi65 – 695Combined sources
Helixi71 – 9424Combined sources
Helixi101 – 11919Combined sources
Helixi124 – 14219Combined sources
Helixi150 – 17829Combined sources
Beta strandi179 – 1813Combined sources
Helixi185 – 1906Combined sources
Beta strandi192 – 1954Combined sources
Helixi199 – 21618Combined sources
Helixi222 – 23110Combined sources
Helixi234 – 2418Combined sources
Helixi246 – 25510Combined sources
Helixi257 – 2604Combined sources
Helixi264 – 28320Combined sources
Turni284 – 2874Combined sources
Helixi291 – 31121Combined sources
Helixi315 – 34228Combined sources
Turni356 – 3583Combined sources
Helixi362 – 37413Combined sources
Beta strandi377 – 3793Combined sources
Helixi380 – 39415Combined sources
Beta strandi395 – 3973Combined sources
Helixi400 – 42526Combined sources
Beta strandi426 – 4283Combined sources
Helixi440 – 45516Combined sources
Helixi457 – 4593Combined sources
Helixi470 – 4767Combined sources
Helixi489 – 51123Combined sources
Helixi519 – 5213Combined sources
Helixi522 – 5254Combined sources
Turni526 – 5305Combined sources
Helixi531 – 5333Combined sources
Helixi535 – 5384Combined sources
Turni539 – 5424Combined sources
Helixi543 – 57836Combined sources
Helixi586 – 60419Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HE8X-ray3.30F/L1-606[»]
4HEAX-ray3.30L/T1-606[»]
ProteinModelPortaliQ56227.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I subunit 5 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1009.
HOGENOMiHOG000100570.
KOiK00341.
OMAiDWLTKDV.
OrthoDBiEOG6FZ48R.
PhylomeDBiQ56227.

Family and domain databases

InterProiIPR001750. NADH_UbQ/plastoQ_OxRdtase.
IPR001516. NADH_UbQ_OxRdtase_chain5/L_N.
IPR003945. NADHpl_OxRdtase_5.
IPR018393. NADHpl_OxRdtase_5_subgr.
[Graphical view]
PfamiPF00361. Oxidored_q1. 1 hit.
PF00662. Oxidored_q1_N. 1 hit.
[Graphical view]
PRINTSiPR01435. NPOXDRDTASE5.
TIGRFAMsiTIGR01974. NDH_I_L. 1 hit.

Sequencei

Sequence statusi: Complete.

Q56227-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALLGTILLP LLGFALLGLF GKRMREPLPG VLASGLVLAS FLLGAGLLLS
60 70 80 90 100
GGARFQAEWL PGIPFSLLLD NLSGFMLLIV TGVGFLIHVY AIGYMGGDPG
110 120 130 140 150
YSRFFAYFNL FIAMMLTLVL ADSYPVMFIG WEGVGLASFL LIGFWYKNPQ
160 170 180 190 200
YADSARKAFI VNRIGDLGFM LGMAILWALY GTLSISELKE AMEGPLKNPD
210 220 230 240 250
LLALAGLLLF LGAVGKSAQI PLMVWLPDAM AGPTPVSALI HAATMVTAGV
260 270 280 290 300
YLIARSSFLY SVLPDVSYAI AVVGLLTAAY GALSAFGQTD IKKIVAYSTI
310 320 330 340 350
SQLGYMFLAA GVGAYWVALF HVFTHAFFKA LLFLASGSVI HALGGEQDVR
360 370 380 390 400
KMGGLWKHLP QTRWHALIGA LALGGLPLLS GFWSKDAILA ATLTYPFGGV
410 420 430 440 450
GFYVGALLVA VLTAMYAMRW FVLVFLGEER GHHHPHEAPP VMLWPNHLLA
460 470 480 490 500
LGSVLAGYLA LPHPLPNVLE PFLKPALAEV EAHHLSLGAE WGLIALSAAV
510 520 530 540 550
ALLGLWAGFV FFQRKVFPAW YLAFEAASRE AFYVDRAYNA LIVNPLKALA
560 570 580 590 600
EALFYGDRGL LSGYFGLGGA ARSLGQGLAR LQTGYLRVYA LLFVLGALLL

LGVMRW
Length:606
Mass (Da):65,141
Last modified:November 1, 1997 - v1
Checksum:i47318E8F2665295C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52917 Genomic DNA. Translation: AAA97949.1.
AP008226 Genomic DNA. Translation: BAD69918.1.
PIRiT11909.
RefSeqiWP_011227705.1. NC_006461.1.
YP_143361.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD69918; BAD69918; BAD69918.
GeneIDi3169613.
KEGGittj:TTHA0095.
PATRICi23955121. VBITheThe93045_0093.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52917 Genomic DNA. Translation: AAA97949.1 .
AP008226 Genomic DNA. Translation: BAD69918.1 .
PIRi T11909.
RefSeqi WP_011227705.1. NC_006461.1.
YP_143361.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4HE8 X-ray 3.30 F/L 1-606 [» ]
4HEA X-ray 3.30 L/T 1-606 [» ]
ProteinModelPortali Q56227.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-59270N.
STRINGi 300852.TTHA0095.

Protein family/group databases

TCDBi 3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD69918 ; BAD69918 ; BAD69918 .
GeneIDi 3169613.
KEGGi ttj:TTHA0095.
PATRICi 23955121. VBITheThe93045_0093.

Phylogenomic databases

eggNOGi COG1009.
HOGENOMi HOG000100570.
KOi K00341.
OMAi DWLTKDV.
OrthoDBi EOG6FZ48R.
PhylomeDBi Q56227.

Enzyme and pathway databases

BioCyci TTHE300852:GH8R-99-MONOMER.

Family and domain databases

InterProi IPR001750. NADH_UbQ/plastoQ_OxRdtase.
IPR001516. NADH_UbQ_OxRdtase_chain5/L_N.
IPR003945. NADHpl_OxRdtase_5.
IPR018393. NADHpl_OxRdtase_5_subgr.
[Graphical view ]
Pfami PF00361. Oxidored_q1. 1 hit.
PF00662. Oxidored_q1_N. 1 hit.
[Graphical view ]
PRINTSi PR01435. NPOXDRDTASE5.
TIGRFAMsi TIGR01974. NDH_I_L. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit."
    Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T.
    J. Biol. Chem. 272:4201-4211(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.

Entry informationi

Entry nameiNQO12_THET8
AccessioniPrimary (citable) accession number: Q56227
Secondary accession number(s): Q5SM48
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 26, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3