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Q56227 (NQO12_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH-quinone oxidoreductase subunit 12

EC=1.6.99.5
Alternative name(s):
NADH dehydrogenase I chain 12
NDH-1 subunit 12
Gene names
Name:nqo12
Ordered Locus Names:TTHA0095
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length606 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.

Catalytic activity

NADH + quinone = NAD+ + quinol.

Subunit structure

NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.

Subcellular location

Cell inner membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the complex I subunit 5 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 606606NADH-quinone oxidoreductase subunit 12
PRO_0000118212

Regions

Transmembrane1 – 2121Helical; Potential
Transmembrane29 – 4921Helical; Potential
Transmembrane75 – 9521Helical; Potential
Transmembrane104 – 12421Helical; Potential
Transmembrane126 – 14621Helical; Potential
Transmembrane165 – 18521Helical; Potential
Transmembrane206 – 22621Helical; Potential
Transmembrane234 – 25421Helical; Potential
Transmembrane266 – 28621Helical; Potential
Transmembrane303 – 32321Helical; Potential
Transmembrane325 – 34521Helical; Potential
Transmembrane364 – 38421Helical; Potential
Transmembrane397 – 41721Helical; Potential
Transmembrane441 – 46121Helical; Potential
Transmembrane492 – 51221Helical; Potential
Transmembrane584 – 60421Helical; Potential

Secondary structure

....................................................................... 606
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q56227 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 47318E8F2665295C

FASTA60665,141
        10         20         30         40         50         60 
MALLGTILLP LLGFALLGLF GKRMREPLPG VLASGLVLAS FLLGAGLLLS GGARFQAEWL 

        70         80         90        100        110        120 
PGIPFSLLLD NLSGFMLLIV TGVGFLIHVY AIGYMGGDPG YSRFFAYFNL FIAMMLTLVL 

       130        140        150        160        170        180 
ADSYPVMFIG WEGVGLASFL LIGFWYKNPQ YADSARKAFI VNRIGDLGFM LGMAILWALY 

       190        200        210        220        230        240 
GTLSISELKE AMEGPLKNPD LLALAGLLLF LGAVGKSAQI PLMVWLPDAM AGPTPVSALI 

       250        260        270        280        290        300 
HAATMVTAGV YLIARSSFLY SVLPDVSYAI AVVGLLTAAY GALSAFGQTD IKKIVAYSTI 

       310        320        330        340        350        360 
SQLGYMFLAA GVGAYWVALF HVFTHAFFKA LLFLASGSVI HALGGEQDVR KMGGLWKHLP 

       370        380        390        400        410        420 
QTRWHALIGA LALGGLPLLS GFWSKDAILA ATLTYPFGGV GFYVGALLVA VLTAMYAMRW 

       430        440        450        460        470        480 
FVLVFLGEER GHHHPHEAPP VMLWPNHLLA LGSVLAGYLA LPHPLPNVLE PFLKPALAEV 

       490        500        510        520        530        540 
EAHHLSLGAE WGLIALSAAV ALLGLWAGFV FFQRKVFPAW YLAFEAASRE AFYVDRAYNA 

       550        560        570        580        590        600 
LIVNPLKALA EALFYGDRGL LSGYFGLGGA ARSLGQGLAR LQTGYLRVYA LLFVLGALLL 


LGVMRW 

« Hide

References

« Hide 'large scale' references
[1]"The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit."
Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T.
J. Biol. Chem. 272:4201-4211(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U52917 Genomic DNA. Translation: AAA97949.1.
AP008226 Genomic DNA. Translation: BAD69918.1.
PIRT11909.
RefSeqYP_143361.1. NC_006461.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4HE8X-ray3.30F/L1-606[»]
4HEAX-ray3.30L/T1-606[»]
ProteinModelPortalQ56227.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-59270N.
STRING300852.TTHA0095.

Protein family/group databases

TCDB3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD69918; BAD69918; BAD69918.
GeneID3169613.
KEGGttj:TTHA0095.
PATRIC23955121. VBITheThe93045_0093.

Phylogenomic databases

eggNOGCOG1009.
HOGENOMHOG000100570.
KOK00341.
OMADWLTKDV.
OrthoDBEOG6FZ48R.
PhylomeDBQ56227.

Enzyme and pathway databases

BioCycTTHE300852:GH8R-99-MONOMER.

Family and domain databases

InterProIPR001750. NADH_UbQ/plastoQ_OxRdtase.
IPR001516. NADH_UbQ_OxRdtase_chain5/L_N.
IPR003945. NADHpl_OxRdtase_5.
IPR018393. NADHpl_OxRdtase_5_subgr.
[Graphical view]
PfamPF00361. Oxidored_q1. 1 hit.
PF00662. Oxidored_q1_N. 1 hit.
[Graphical view]
PRINTSPR01435. NPOXDRDTASE5.
TIGRFAMsTIGR01974. NDH_I_L. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNQO12_THET8
AccessionPrimary (citable) accession number: Q56227
Secondary accession number(s): Q5SM48
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references