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Q56227

- NQO12_THET8

UniProt

Q56227 - NQO12_THET8

Protein

NADH-quinone oxidoreductase subunit 12

Gene

nqo12

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.

    Catalytic activityi

    NADH + quinone = NAD+ + quinol.

    GO - Molecular functioni

    1. NADH dehydrogenase (ubiquinone) activity Source: InterPro
    2. quinone binding Source: UniProtKB-KW

    GO - Biological processi

    1. ATP synthesis coupled electron transport Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciTTHE300852:GH8R-99-MONOMER.

    Protein family/group databases

    TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH-quinone oxidoreductase subunit 12 (EC:1.6.99.5)
    Alternative name(s):
    NADH dehydrogenase I chain 12
    NDH-1 subunit 12
    Gene namesi
    Name:nqo12
    Ordered Locus Names:TTHA0095
    OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
    Taxonomic identifieri300852 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
    ProteomesiUP000000532: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 606606NADH-quinone oxidoreductase subunit 12PRO_0000118212Add
    BLAST

    Keywords - PTMi

    Quinone

    Interactioni

    Subunit structurei

    NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.

    Protein-protein interaction databases

    DIPiDIP-59270N.
    STRINGi300852.TTHA0095.

    Structurei

    Secondary structure

    1
    606
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi2 – 1211
    Helixi14 – 196
    Helixi28 – 4922
    Beta strandi53 – 575
    Beta strandi65 – 695
    Helixi71 – 9424
    Helixi101 – 11919
    Helixi124 – 14219
    Helixi150 – 17829
    Beta strandi179 – 1813
    Helixi185 – 1906
    Beta strandi192 – 1954
    Helixi199 – 21618
    Helixi222 – 23110
    Helixi234 – 2418
    Helixi246 – 25510
    Helixi257 – 2604
    Helixi264 – 28320
    Turni284 – 2874
    Helixi291 – 31121
    Helixi315 – 34228
    Turni356 – 3583
    Helixi362 – 37413
    Beta strandi377 – 3793
    Helixi380 – 39415
    Beta strandi395 – 3973
    Helixi400 – 42526
    Beta strandi426 – 4283
    Helixi440 – 45516
    Helixi457 – 4593
    Helixi470 – 4767
    Helixi489 – 51123
    Helixi519 – 5213
    Helixi522 – 5254
    Turni526 – 5305
    Helixi531 – 5333
    Helixi535 – 5384
    Turni539 – 5424
    Helixi543 – 57836
    Helixi586 – 60419

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4HE8X-ray3.30F/L1-606[»]
    4HEAX-ray3.30L/T1-606[»]
    ProteinModelPortaliQ56227.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1 – 2121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei29 – 4921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei75 – 9521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei104 – 12421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei126 – 14621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei165 – 18521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei206 – 22621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei234 – 25421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei266 – 28621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei303 – 32321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei325 – 34521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei364 – 38421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei397 – 41721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei441 – 46121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei492 – 51221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei584 – 60421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the complex I subunit 5 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1009.
    HOGENOMiHOG000100570.
    KOiK00341.
    OMAiDWLTKDV.
    OrthoDBiEOG6FZ48R.
    PhylomeDBiQ56227.

    Family and domain databases

    InterProiIPR001750. NADH_UbQ/plastoQ_OxRdtase.
    IPR001516. NADH_UbQ_OxRdtase_chain5/L_N.
    IPR003945. NADHpl_OxRdtase_5.
    IPR018393. NADHpl_OxRdtase_5_subgr.
    [Graphical view]
    PfamiPF00361. Oxidored_q1. 1 hit.
    PF00662. Oxidored_q1_N. 1 hit.
    [Graphical view]
    PRINTSiPR01435. NPOXDRDTASE5.
    TIGRFAMsiTIGR01974. NDH_I_L. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q56227-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALLGTILLP LLGFALLGLF GKRMREPLPG VLASGLVLAS FLLGAGLLLS    50
    GGARFQAEWL PGIPFSLLLD NLSGFMLLIV TGVGFLIHVY AIGYMGGDPG 100
    YSRFFAYFNL FIAMMLTLVL ADSYPVMFIG WEGVGLASFL LIGFWYKNPQ 150
    YADSARKAFI VNRIGDLGFM LGMAILWALY GTLSISELKE AMEGPLKNPD 200
    LLALAGLLLF LGAVGKSAQI PLMVWLPDAM AGPTPVSALI HAATMVTAGV 250
    YLIARSSFLY SVLPDVSYAI AVVGLLTAAY GALSAFGQTD IKKIVAYSTI 300
    SQLGYMFLAA GVGAYWVALF HVFTHAFFKA LLFLASGSVI HALGGEQDVR 350
    KMGGLWKHLP QTRWHALIGA LALGGLPLLS GFWSKDAILA ATLTYPFGGV 400
    GFYVGALLVA VLTAMYAMRW FVLVFLGEER GHHHPHEAPP VMLWPNHLLA 450
    LGSVLAGYLA LPHPLPNVLE PFLKPALAEV EAHHLSLGAE WGLIALSAAV 500
    ALLGLWAGFV FFQRKVFPAW YLAFEAASRE AFYVDRAYNA LIVNPLKALA 550
    EALFYGDRGL LSGYFGLGGA ARSLGQGLAR LQTGYLRVYA LLFVLGALLL 600
    LGVMRW 606
    Length:606
    Mass (Da):65,141
    Last modified:November 1, 1997 - v1
    Checksum:i47318E8F2665295C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U52917 Genomic DNA. Translation: AAA97949.1.
    AP008226 Genomic DNA. Translation: BAD69918.1.
    PIRiT11909.
    RefSeqiWP_011227705.1. NC_006461.1.
    YP_143361.1. NC_006461.1.

    Genome annotation databases

    EnsemblBacteriaiBAD69918; BAD69918; BAD69918.
    GeneIDi3169613.
    KEGGittj:TTHA0095.
    PATRICi23955121. VBITheThe93045_0093.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U52917 Genomic DNA. Translation: AAA97949.1 .
    AP008226 Genomic DNA. Translation: BAD69918.1 .
    PIRi T11909.
    RefSeqi WP_011227705.1. NC_006461.1.
    YP_143361.1. NC_006461.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4HE8 X-ray 3.30 F/L 1-606 [» ]
    4HEA X-ray 3.30 L/T 1-606 [» ]
    ProteinModelPortali Q56227.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-59270N.
    STRINGi 300852.TTHA0095.

    Protein family/group databases

    TCDBi 3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD69918 ; BAD69918 ; BAD69918 .
    GeneIDi 3169613.
    KEGGi ttj:TTHA0095.
    PATRICi 23955121. VBITheThe93045_0093.

    Phylogenomic databases

    eggNOGi COG1009.
    HOGENOMi HOG000100570.
    KOi K00341.
    OMAi DWLTKDV.
    OrthoDBi EOG6FZ48R.
    PhylomeDBi Q56227.

    Enzyme and pathway databases

    BioCyci TTHE300852:GH8R-99-MONOMER.

    Family and domain databases

    InterProi IPR001750. NADH_UbQ/plastoQ_OxRdtase.
    IPR001516. NADH_UbQ_OxRdtase_chain5/L_N.
    IPR003945. NADHpl_OxRdtase_5.
    IPR018393. NADHpl_OxRdtase_5_subgr.
    [Graphical view ]
    Pfami PF00361. Oxidored_q1. 1 hit.
    PF00662. Oxidored_q1_N. 1 hit.
    [Graphical view ]
    PRINTSi PR01435. NPOXDRDTASE5.
    TIGRFAMsi TIGR01974. NDH_I_L. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit."
      Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T.
      J. Biol. Chem. 272:4201-4211(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete genome sequence of Thermus thermophilus HB8."
      Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HB8 / ATCC 27634 / DSM 579.

    Entry informationi

    Entry nameiNQO12_THET8
    AccessioniPrimary (citable) accession number: Q56227
    Secondary accession number(s): Q5SM48
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3