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Q56227

- NQO12_THET8

UniProt

Q56227 - NQO12_THET8

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Protein
NADH-quinone oxidoreductase subunit 12
Gene
nqo12, TTHA0095
Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.

Catalytic activityi

NADH + quinone = NAD+ + quinol.

GO - Molecular functioni

  1. NADH dehydrogenase (ubiquinone) activity Source: InterPro
  2. quinone binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. ATP synthesis coupled electron transport Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-99-MONOMER.

Protein family/group databases

TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit 12 (EC:1.6.99.5)
Alternative name(s):
NADH dehydrogenase I chain 12
NDH-1 subunit 12
Gene namesi
Name:nqo12
Ordered Locus Names:TTHA0095
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
ProteomesiUP000000532: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1 – 2121Helical; Reviewed prediction
Add
BLAST
Transmembranei29 – 4921Helical; Reviewed prediction
Add
BLAST
Transmembranei75 – 9521Helical; Reviewed prediction
Add
BLAST
Transmembranei104 – 12421Helical; Reviewed prediction
Add
BLAST
Transmembranei126 – 14621Helical; Reviewed prediction
Add
BLAST
Transmembranei165 – 18521Helical; Reviewed prediction
Add
BLAST
Transmembranei206 – 22621Helical; Reviewed prediction
Add
BLAST
Transmembranei234 – 25421Helical; Reviewed prediction
Add
BLAST
Transmembranei266 – 28621Helical; Reviewed prediction
Add
BLAST
Transmembranei303 – 32321Helical; Reviewed prediction
Add
BLAST
Transmembranei325 – 34521Helical; Reviewed prediction
Add
BLAST
Transmembranei364 – 38421Helical; Reviewed prediction
Add
BLAST
Transmembranei397 – 41721Helical; Reviewed prediction
Add
BLAST
Transmembranei441 – 46121Helical; Reviewed prediction
Add
BLAST
Transmembranei492 – 51221Helical; Reviewed prediction
Add
BLAST
Transmembranei584 – 60421Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 606606NADH-quinone oxidoreductase subunit 12
PRO_0000118212Add
BLAST

Keywords - PTMi

Quinone

Interactioni

Subunit structurei

NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.

Protein-protein interaction databases

DIPiDIP-59270N.
STRINGi300852.TTHA0095.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 1211
Helixi14 – 196
Helixi28 – 4922
Beta strandi53 – 575
Beta strandi65 – 695
Helixi71 – 9424
Helixi101 – 11919
Helixi124 – 14219
Helixi150 – 17829
Beta strandi179 – 1813
Helixi185 – 1906
Beta strandi192 – 1954
Helixi199 – 21618
Helixi222 – 23110
Helixi234 – 2418
Helixi246 – 25510
Helixi257 – 2604
Helixi264 – 28320
Turni284 – 2874
Helixi291 – 31121
Helixi315 – 34228
Turni356 – 3583
Helixi362 – 37413
Beta strandi377 – 3793
Helixi380 – 39415
Beta strandi395 – 3973
Helixi400 – 42526
Beta strandi426 – 4283
Helixi440 – 45516
Helixi457 – 4593
Helixi470 – 4767
Helixi489 – 51123
Helixi519 – 5213
Helixi522 – 5254
Turni526 – 5305
Helixi531 – 5333
Helixi535 – 5384
Turni539 – 5424
Helixi543 – 57836
Helixi586 – 60419

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HE8X-ray3.30F/L1-606[»]
4HEAX-ray3.30L/T1-606[»]
ProteinModelPortaliQ56227.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1009.
HOGENOMiHOG000100570.
KOiK00341.
OMAiDWLTKDV.
OrthoDBiEOG6FZ48R.
PhylomeDBiQ56227.

Family and domain databases

InterProiIPR001750. NADH_UbQ/plastoQ_OxRdtase.
IPR001516. NADH_UbQ_OxRdtase_chain5/L_N.
IPR003945. NADHpl_OxRdtase_5.
IPR018393. NADHpl_OxRdtase_5_subgr.
[Graphical view]
PfamiPF00361. Oxidored_q1. 1 hit.
PF00662. Oxidored_q1_N. 1 hit.
[Graphical view]
PRINTSiPR01435. NPOXDRDTASE5.
TIGRFAMsiTIGR01974. NDH_I_L. 1 hit.

Sequencei

Sequence statusi: Complete.

Q56227-1 [UniParc]FASTAAdd to Basket

« Hide

MALLGTILLP LLGFALLGLF GKRMREPLPG VLASGLVLAS FLLGAGLLLS    50
GGARFQAEWL PGIPFSLLLD NLSGFMLLIV TGVGFLIHVY AIGYMGGDPG 100
YSRFFAYFNL FIAMMLTLVL ADSYPVMFIG WEGVGLASFL LIGFWYKNPQ 150
YADSARKAFI VNRIGDLGFM LGMAILWALY GTLSISELKE AMEGPLKNPD 200
LLALAGLLLF LGAVGKSAQI PLMVWLPDAM AGPTPVSALI HAATMVTAGV 250
YLIARSSFLY SVLPDVSYAI AVVGLLTAAY GALSAFGQTD IKKIVAYSTI 300
SQLGYMFLAA GVGAYWVALF HVFTHAFFKA LLFLASGSVI HALGGEQDVR 350
KMGGLWKHLP QTRWHALIGA LALGGLPLLS GFWSKDAILA ATLTYPFGGV 400
GFYVGALLVA VLTAMYAMRW FVLVFLGEER GHHHPHEAPP VMLWPNHLLA 450
LGSVLAGYLA LPHPLPNVLE PFLKPALAEV EAHHLSLGAE WGLIALSAAV 500
ALLGLWAGFV FFQRKVFPAW YLAFEAASRE AFYVDRAYNA LIVNPLKALA 550
EALFYGDRGL LSGYFGLGGA ARSLGQGLAR LQTGYLRVYA LLFVLGALLL 600
LGVMRW 606
Length:606
Mass (Da):65,141
Last modified:November 1, 1997 - v1
Checksum:i47318E8F2665295C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U52917 Genomic DNA. Translation: AAA97949.1.
AP008226 Genomic DNA. Translation: BAD69918.1.
PIRiT11909.
RefSeqiWP_011227705.1. NC_006461.1.
YP_143361.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD69918; BAD69918; BAD69918.
GeneIDi3169613.
KEGGittj:TTHA0095.
PATRICi23955121. VBITheThe93045_0093.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U52917 Genomic DNA. Translation: AAA97949.1 .
AP008226 Genomic DNA. Translation: BAD69918.1 .
PIRi T11909.
RefSeqi WP_011227705.1. NC_006461.1.
YP_143361.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4HE8 X-ray 3.30 F/L 1-606 [» ]
4HEA X-ray 3.30 L/T 1-606 [» ]
ProteinModelPortali Q56227.
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-59270N.
STRINGi 300852.TTHA0095.

Protein family/group databases

TCDBi 3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD69918 ; BAD69918 ; BAD69918 .
GeneIDi 3169613.
KEGGi ttj:TTHA0095.
PATRICi 23955121. VBITheThe93045_0093.

Phylogenomic databases

eggNOGi COG1009.
HOGENOMi HOG000100570.
KOi K00341.
OMAi DWLTKDV.
OrthoDBi EOG6FZ48R.
PhylomeDBi Q56227.

Enzyme and pathway databases

BioCyci TTHE300852:GH8R-99-MONOMER.

Family and domain databases

InterProi IPR001750. NADH_UbQ/plastoQ_OxRdtase.
IPR001516. NADH_UbQ_OxRdtase_chain5/L_N.
IPR003945. NADHpl_OxRdtase_5.
IPR018393. NADHpl_OxRdtase_5_subgr.
[Graphical view ]
Pfami PF00361. Oxidored_q1. 1 hit.
PF00662. Oxidored_q1_N. 1 hit.
[Graphical view ]
PRINTSi PR01435. NPOXDRDTASE5.
TIGRFAMsi TIGR01974. NDH_I_L. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit."
    Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T.
    J. Biol. Chem. 272:4201-4211(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.

Entry informationi

Entry nameiNQO12_THET8
AccessioniPrimary (citable) accession number: Q56227
Secondary accession number(s): Q5SM48
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: September 3, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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