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Q56226 (NQO11_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH-quinone oxidoreductase subunit 11

EC=1.6.99.5
Alternative name(s):
NADH dehydrogenase I chain 11
NDH-1 subunit 11
Gene names
Name:nqo11
Ordered Locus Names:TTHA0094
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length95 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. HAMAP-Rule MF_01456

Catalytic activity

NADH + quinone = NAD+ + quinol. HAMAP-Rule MF_01456

Subunit structure

NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.

Subcellular location

Cell inner membrane; Multi-pass membrane protein HAMAP-Rule MF_01456.

Sequence similarities

Belongs to the complex I subunit 4L family.

Ontologies

Keywords
   Biological processTransport
   Cellular componentCell inner membrane
Cell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   LigandNAD
   Molecular functionOxidoreductase
   PTMQuinone
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processATP synthesis coupled electron transport

Inferred from electronic annotation. Source: InterPro

transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionNADH dehydrogenase (quinone) activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

quinone binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 9595NADH-quinone oxidoreductase subunit 11 HAMAP-Rule MF_01456
PRO_0000118523

Regions

Transmembrane1 – 2121Helical; Potential
Transmembrane25 – 4521Helical; Potential
Transmembrane59 – 7921Helical; Potential

Secondary structure

.......... 95
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q56226 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: ED9D2A80D2E96592

FASTA959,996
        10         20         30         40         50         60 
MSYLLTSALL FALGVYGVLT RRTAILVFLS IELMLNAANL SLVGFARAYG LDGQVAALMV 

        70         80         90 
IAVAAAEVAV GLGLIVAIFR HRESTAVDDL SELRG 

« Hide

References

« Hide 'large scale' references
[1]"The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit."
Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T.
J. Biol. Chem. 272:4201-4211(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U52917 Genomic DNA. Translation: AAA97948.1.
AP008226 Genomic DNA. Translation: BAD69917.1.
PIRT11908.
RefSeqYP_143360.1. NC_006461.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4HE8X-ray3.30E/K1-95[»]
4HEAX-ray3.30K/S1-95[»]
ProteinModelPortalQ56226.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-59269N.
STRING300852.TTHA0094.

Protein family/group databases

TCDB3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD69917; BAD69917; BAD69917.
GeneID3169648.
KEGGttj:TTHA0094.
PATRIC23955119. VBITheThe93045_0092.

Phylogenomic databases

eggNOGCOG0713.
HOGENOMHOG000066429.
KOK00340.
OMARVTVDME.
OrthoDBEOG6MSS3R.
ProtClustDBCLSK830158.

Enzyme and pathway databases

BioCycTTHE300852:GH8R-98-MONOMER.

Family and domain databases

HAMAPMF_01456. NDH1_NuoK.
InterProIPR001133. NADH_UbQ_OxRdtase_chain4L/K.
[Graphical view]
PANTHERPTHR11434. PTHR11434. 1 hit.
PfamPF00420. Oxidored_q2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNQO11_THET8
AccessionPrimary (citable) accession number: Q56226
Secondary accession number(s): Q5SM49
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: February 19, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references