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Q56225

- NQO10_THET8

UniProt

Q56225 - NQO10_THET8

Protein

NADH-quinone oxidoreductase subunit 10

Gene

nqo10

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.

    Catalytic activityi

    NADH + quinone = NAD+ + quinol.

    GO - Molecular functioni

    1. NADH dehydrogenase (ubiquinone) activity Source: InterPro
    2. quinone binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciTTHE300852:GH8R-97-MONOMER.

    Protein family/group databases

    TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH-quinone oxidoreductase subunit 10 (EC:1.6.99.5)
    Alternative name(s):
    NADH dehydrogenase I chain 10
    NDH-1 subunit 10
    Gene namesi
    Name:nqo10
    Ordered Locus Names:TTHA0093
    OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
    Taxonomic identifieri300852 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
    ProteomesiUP000000532: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 176176NADH-quinone oxidoreductase subunit 10PRO_0000118369Add
    BLAST

    Keywords - PTMi

    Quinone

    Interactioni

    Subunit structurei

    NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.

    Protein-protein interaction databases

    DIPiDIP-59268N.
    STRINGi300852.TTHA0093.

    Structurei

    Secondary structure

    1
    176
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi2 – 2120
    Helixi25 – 4521
    Helixi49 – 579
    Helixi58 – 625
    Helixi63 – 7311
    Beta strandi76 – 794
    Turni87 – 893
    Helixi90 – 10819
    Helixi124 – 1318
    Turni132 – 1354
    Helixi136 – 15520

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4HE8X-ray3.30D/J1-176[»]
    4HEAX-ray3.30J/R1-176[»]
    ProteinModelPortaliQ56225.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei2 – 2221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei26 – 4621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei56 – 7621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei91 – 11121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei137 – 15721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the complex I subunit 6 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG260906.
    HOGENOMiHOG000262591.
    KOiK00339.
    OMAiGLWGLDL.
    OrthoDBiEOG6BCT1X.

    Family and domain databases

    InterProiIPR001457. NADH_UbQ/plastoQ_OxRdtase_su6.
    [Graphical view]
    PfamiPF00499. Oxidored_q3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q56225-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLLEGLALF LLLLSGVLVV TLRNAIHAAL ALILNFLVLA GVYVALDARF    50
    LGFIQVIVYA GAIVVLFLFV IMLLFAAQGE IGFDPLVRSR PLAALLALGV 100
    AGILAAGLWG LDLAFTQDLK GGLPQALGPL LYGDWLFVLL AVGFLLMAAT 150
    VVAVALVEPG KASRAKEAEK REEVAR 176
    Length:176
    Mass (Da):18,551
    Last modified:November 1, 1997 - v1
    Checksum:i802325655FE3AAB1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U52917 Genomic DNA. Translation: AAA97947.1.
    AP008226 Genomic DNA. Translation: BAD69916.1.
    PIRiT11907.
    RefSeqiWP_011227703.1. NC_006461.1.
    YP_143359.1. NC_006461.1.

    Genome annotation databases

    EnsemblBacteriaiBAD69916; BAD69916; BAD69916.
    GeneIDi3169626.
    KEGGittj:TTHA0093.
    PATRICi23955117. VBITheThe93045_0091.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U52917 Genomic DNA. Translation: AAA97947.1 .
    AP008226 Genomic DNA. Translation: BAD69916.1 .
    PIRi T11907.
    RefSeqi WP_011227703.1. NC_006461.1.
    YP_143359.1. NC_006461.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4HE8 X-ray 3.30 D/J 1-176 [» ]
    4HEA X-ray 3.30 J/R 1-176 [» ]
    ProteinModelPortali Q56225.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-59268N.
    STRINGi 300852.TTHA0093.

    Protein family/group databases

    TCDBi 3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD69916 ; BAD69916 ; BAD69916 .
    GeneIDi 3169626.
    KEGGi ttj:TTHA0093.
    PATRICi 23955117. VBITheThe93045_0091.

    Phylogenomic databases

    eggNOGi NOG260906.
    HOGENOMi HOG000262591.
    KOi K00339.
    OMAi GLWGLDL.
    OrthoDBi EOG6BCT1X.

    Enzyme and pathway databases

    BioCyci TTHE300852:GH8R-97-MONOMER.

    Family and domain databases

    InterProi IPR001457. NADH_UbQ/plastoQ_OxRdtase_su6.
    [Graphical view ]
    Pfami PF00499. Oxidored_q3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit."
      Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T.
      J. Biol. Chem. 272:4201-4211(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete genome sequence of Thermus thermophilus HB8."
      Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HB8 / ATCC 27634 / DSM 579.

    Entry informationi

    Entry nameiNQO10_THET8
    AccessioniPrimary (citable) accession number: Q56225
    Secondary accession number(s): Q5SM50
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3