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Protein

NADH-quinone oxidoreductase subunit 10

Gene

nqo10

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.

Catalytic activityi

NADH + quinone = NAD+ + quinol.

GO - Molecular functioni

  1. NADH dehydrogenase (ubiquinone) activity Source: InterPro
  2. quinone binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-97-MONOMER.

Protein family/group databases

TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit 10 (EC:1.6.99.5)
Alternative name(s):
NADH dehydrogenase I chain 10
NDH-1 subunit 10
Gene namesi
Name:nqo10
Ordered Locus Names:TTHA0093
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
ProteomesiUP000000532 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei2 – 2221HelicalSequence AnalysisAdd
BLAST
Transmembranei26 – 4621HelicalSequence AnalysisAdd
BLAST
Transmembranei56 – 7621HelicalSequence AnalysisAdd
BLAST
Transmembranei91 – 11121HelicalSequence AnalysisAdd
BLAST
Transmembranei137 – 15721HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 176176NADH-quinone oxidoreductase subunit 10PRO_0000118369Add
BLAST

Keywords - PTMi

Quinone

Interactioni

Subunit structurei

NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.

Protein-protein interaction databases

DIPiDIP-59268N.
STRINGi300852.TTHA0093.

Structurei

Secondary structure

1
176
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 2120Combined sources
Helixi25 – 4521Combined sources
Helixi49 – 579Combined sources
Helixi58 – 625Combined sources
Helixi63 – 7311Combined sources
Beta strandi76 – 794Combined sources
Turni87 – 893Combined sources
Helixi90 – 10819Combined sources
Helixi124 – 1318Combined sources
Turni132 – 1354Combined sources
Helixi136 – 15520Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HE8X-ray3.30D/J1-176[»]
4HEAX-ray3.30J/R1-176[»]
ProteinModelPortaliQ56225.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I subunit 6 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG260906.
HOGENOMiHOG000262591.
KOiK00339.
OMAiLLYGDWL.
OrthoDBiEOG6BCT1X.

Family and domain databases

InterProiIPR001457. NADH_UbQ/plastoQ_OxRdtase_su6.
[Graphical view]
PfamiPF00499. Oxidored_q3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q56225-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLLEGLALF LLLLSGVLVV TLRNAIHAAL ALILNFLVLA GVYVALDARF
60 70 80 90 100
LGFIQVIVYA GAIVVLFLFV IMLLFAAQGE IGFDPLVRSR PLAALLALGV
110 120 130 140 150
AGILAAGLWG LDLAFTQDLK GGLPQALGPL LYGDWLFVLL AVGFLLMAAT
160 170
VVAVALVEPG KASRAKEAEK REEVAR
Length:176
Mass (Da):18,551
Last modified:November 1, 1997 - v1
Checksum:i802325655FE3AAB1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52917 Genomic DNA. Translation: AAA97947.1.
AP008226 Genomic DNA. Translation: BAD69916.1.
PIRiT11907.
RefSeqiWP_011227703.1. NC_006461.1.
YP_143359.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD69916; BAD69916; BAD69916.
GeneIDi3169626.
KEGGittj:TTHA0093.
PATRICi23955117. VBITheThe93045_0091.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52917 Genomic DNA. Translation: AAA97947.1.
AP008226 Genomic DNA. Translation: BAD69916.1.
PIRiT11907.
RefSeqiWP_011227703.1. NC_006461.1.
YP_143359.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HE8X-ray3.30D/J1-176[»]
4HEAX-ray3.30J/R1-176[»]
ProteinModelPortaliQ56225.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59268N.
STRINGi300852.TTHA0093.

Protein family/group databases

TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD69916; BAD69916; BAD69916.
GeneIDi3169626.
KEGGittj:TTHA0093.
PATRICi23955117. VBITheThe93045_0091.

Phylogenomic databases

eggNOGiNOG260906.
HOGENOMiHOG000262591.
KOiK00339.
OMAiLLYGDWL.
OrthoDBiEOG6BCT1X.

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-97-MONOMER.

Family and domain databases

InterProiIPR001457. NADH_UbQ/plastoQ_OxRdtase_su6.
[Graphical view]
PfamiPF00499. Oxidored_q3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit."
    Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T.
    J. Biol. Chem. 272:4201-4211(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.

Entry informationi

Entry nameiNQO10_THET8
AccessioniPrimary (citable) accession number: Q56225
Secondary accession number(s): Q5SM50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: January 7, 2015
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.