Reviewed, UniProtKB/Swiss-Prot Q56225 (NQO10_THET8)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit 10
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I chain 10
    NDH-1 subunit 10

Gene names

Name:	nqo10
Ordered Locus Names:	TTHA0093

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Complete proteome] [HAMAP]

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	176 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Subunit structure	NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the complex I subunit 6 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 176	176	NADH-quinone oxidoreductase subunit 10		PRO_0000118369
Regions
	Transmembrane	2 – 22	21	Potential
	Transmembrane	26 – 46	21	Potential
	Transmembrane	56 – 76	21	Potential
	Transmembrane	91 – 111	21	Potential
	Transmembrane	137 – 157	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q56225-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 802325655FE3AAB1	FASTA	176	18,551
	10 20 30 40 50 60 MSLLEGLALF LLLLSGVLVV TLRNAIHAAL ALILNFLVLA GVYVALDARF LGFIQVIVYA 70 80 90 100 110 120 GAIVVLFLFV IMLLFAAQGE IGFDPLVRSR PLAALLALGV AGILAAGLWG LDLAFTQDLK 130 140 150 160 170 GGLPQALGPL LYGDWLFVLL AVGFLLMAAT VVAVALVEPG KASRAKEAEK REEVAR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit." Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T. J. Biol. Chem. 272:4201-4211(1997) [PubMed: 9020134] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U52917 Genomic DNA. Translation: AAA97947.1. AP008226 Genomic DNA. Translation: BAD69916.1.
PIR	T11907.
RefSeq	YP_143359.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q56225.
Protein family/group databases
TCDB	3.D.1.3.1. proton-translocating NADH dehydrogenase (NDH) family.
Genome annotation databases
GeneID	3169626.
GenomeReviews	Gene locus TTHA0093 in contig AP008226_GR.
KEGG	ttj:TTHA0093.
NMPDR	fig|300852.3.peg.124.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q56225.
OMA	LMAATVV.
Enzyme and pathway databases
BioCyc	TTHE300852:TTHA0093-MON.
Family and domain databases
InterPro	IPR001457. NADH_UbQ/plastoQ_OxRdtase_su6. [Graphical view]
Pfam	PF00499. Oxidored_q3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NQO10_THET8

Accession

Primary (citable) accession number: Q56225
Secondary accession number(s): Q5SM50

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 3, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)