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Q56225 (NQO10_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH-quinone oxidoreductase subunit 10

EC=1.6.99.5
Alternative name(s):
NADH dehydrogenase I chain 10
NDH-1 subunit 10
Gene names
Name:nqo10
Ordered Locus Names:TTHA0093
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length176 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.

Catalytic activity

NADH + quinone = NAD+ + quinol.

Subunit structure

NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.

Subcellular location

Cell inner membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the complex I subunit 6 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 176176NADH-quinone oxidoreductase subunit 10
PRO_0000118369

Regions

Transmembrane2 – 2221Helical; Potential
Transmembrane26 – 4621Helical; Potential
Transmembrane56 – 7621Helical; Potential
Transmembrane91 – 11121Helical; Potential
Transmembrane137 – 15721Helical; Potential

Secondary structure

.................. 176
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q56225 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 802325655FE3AAB1

FASTA17618,551
        10         20         30         40         50         60 
MSLLEGLALF LLLLSGVLVV TLRNAIHAAL ALILNFLVLA GVYVALDARF LGFIQVIVYA 

        70         80         90        100        110        120 
GAIVVLFLFV IMLLFAAQGE IGFDPLVRSR PLAALLALGV AGILAAGLWG LDLAFTQDLK 

       130        140        150        160        170 
GGLPQALGPL LYGDWLFVLL AVGFLLMAAT VVAVALVEPG KASRAKEAEK REEVAR 

« Hide

References

« Hide 'large scale' references
[1]"The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit."
Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T.
J. Biol. Chem. 272:4201-4211(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U52917 Genomic DNA. Translation: AAA97947.1.
AP008226 Genomic DNA. Translation: BAD69916.1.
PIRT11907.
RefSeqYP_143359.1. NC_006461.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4HE8X-ray3.30D/J1-176[»]
4HEAX-ray3.30J/R1-176[»]
ProteinModelPortalQ56225.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-59268N.
STRING300852.TTHA0093.

Protein family/group databases

TCDB3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD69916; BAD69916; BAD69916.
GeneID3169626.
KEGGttj:TTHA0093.
PATRIC23955117. VBITheThe93045_0091.

Phylogenomic databases

eggNOGNOG260906.
HOGENOMHOG000262591.
KOK00339.
OMAGLWGLDL.
OrthoDBEOG6BCT1X.

Enzyme and pathway databases

BioCycTTHE300852:GH8R-97-MONOMER.

Family and domain databases

InterProIPR001457. NADH_UbQ/plastoQ_OxRdtase_su6.
[Graphical view]
PfamPF00499. Oxidored_q3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNQO10_THET8
AccessionPrimary (citable) accession number: Q56225
Secondary accession number(s): Q5SM50
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 14, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references