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Q56224

- NQO9_THET8

UniProt

Q56224 - NQO9_THET8

Protein

NADH-quinone oxidoreductase subunit 9

Gene

nqo9

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. The role of the nqo9 subunit appears to provide a 'connecting chain' of two clusters between cluster N5 and the terminal cluster N2, and to stabilize the structure of the complex by interacting with other subunits.

    Catalytic activityi

    NADH + quinone = NAD+ + quinol.

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. The 4Fe-4S clusters are referred to as N6a and N6b.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi53 – 531Iron-sulfur 1 (4Fe-4S)
    Metal bindingi56 – 561Iron-sulfur 1 (4Fe-4S)
    Metal bindingi59 – 591Iron-sulfur 1 (4Fe-4S)
    Metal bindingi63 – 631Iron-sulfur 2 (4Fe-4S)
    Metal bindingi98 – 981Iron-sulfur 2 (4Fe-4S)
    Metal bindingi101 – 1011Iron-sulfur 2 (4Fe-4S)
    Metal bindingi104 – 1041Iron-sulfur 2 (4Fe-4S)
    Metal bindingi108 – 1081Iron-sulfur 1 (4Fe-4S)

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. iron ion binding Source: UniProtKB-HAMAP
    3. NADH dehydrogenase (quinone) activity Source: UniProtKB-HAMAP
    4. quinone binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD

    Enzyme and pathway databases

    BioCyciTTHE300852:GH8R-96-MONOMER.

    Protein family/group databases

    TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH-quinone oxidoreductase subunit 9 (EC:1.6.99.5)
    Alternative name(s):
    NADH dehydrogenase I subunit 9
    NDH-1 subunit 9
    Gene namesi
    Name:nqo9
    Ordered Locus Names:TTHA0092
    OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
    Taxonomic identifieri300852 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
    ProteomesiUP000000532: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 182182NADH-quinone oxidoreductase subunit 9PRO_0000118721Add
    BLAST

    Keywords - PTMi

    Quinone

    Interactioni

    Subunit structurei

    NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.2 Publications

    Protein-protein interaction databases

    DIPiDIP-59267N.
    STRINGi300852.TTHA0092.

    Structurei

    Secondary structure

    1
    182
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 1815
    Turni26 – 283
    Beta strandi40 – 434
    Helixi58 – 625
    Beta strandi68 – 736
    Beta strandi77 – 793
    Beta strandi81 – 855
    Beta strandi87 – 948
    Turni95 – 973
    Helixi103 – 1075
    Beta strandi109 – 1113
    Beta strandi113 – 1153
    Beta strandi123 – 1253
    Helixi126 – 1294
    Beta strandi130 – 1323
    Helixi133 – 1364
    Helixi144 – 1496
    Turni150 – 1534
    Beta strandi160 – 1623
    Helixi168 – 1703

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2FUGX-ray3.309/G/P/Y1-182[»]
    2YBBelectron microscopy19.0081-182[»]
    3I9VX-ray3.109/G1-182[»]
    3IAMX-ray3.109/G1-182[»]
    3IASX-ray3.159/G/P/Y1-182[»]
    3M9SX-ray4.509/G1-182[»]
    4HEAX-ray3.309/O1-182[»]
    ProteinModelPortaliQ56224.
    SMRiQ56224. Positions 26-179.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ56224.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini43 – 73314Fe-4S ferredoxin-type 1Add
    BLAST
    Domaini89 – 118304Fe-4S ferredoxin-type 2Add
    BLAST

    Sequence similaritiesi

    Belongs to the complex I 23 kDa subunit family.Curated
    Contains 2 4Fe-4S ferredoxin-type domains.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1143.
    HOGENOMiHOG000228291.
    KOiK00338.
    OMAiEFELANY.
    OrthoDBiEOG6XM7H5.
    PhylomeDBiQ56224.

    Family and domain databases

    HAMAPiMF_01351. NDH1_NuoI.
    InterProiIPR001450. 4Fe4S-bd_dom.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR010226. NADH_quinone_OxRdtase_chainI.
    [Graphical view]
    PfamiPF00037. Fer4. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR01971. NuoI. 1 hit.
    PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
    PS51379. 4FE4S_FER_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q56224-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTLKALAQSL GITLKYLFSK PVTVPYPDAP VALKPRFHGR HVLTRHPNGL    50
    EKCIGCSLCA AACPAYAIYV EPAENDPENP VSAGERYAKV YEINMLRCIF 100
    CGLCEEACPT GAIVLGYDFE MADYEYSDLV YGKEDMLVDV VGTKPQRREA 150
    KRTGKPVKVG YVVPYVRPEL EGFKAPTEGG KR 182
    Length:182
    Mass (Da):20,080
    Last modified:November 1, 1997 - v1
    Checksum:i054A9F7942C64C66
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U52917 Genomic DNA. Translation: AAA97946.1.
    AP008226 Genomic DNA. Translation: BAD69915.1.
    PIRiT11906.
    RefSeqiYP_143358.1. NC_006461.1.

    Genome annotation databases

    EnsemblBacteriaiBAD69915; BAD69915; BAD69915.
    GeneIDi3167944.
    KEGGittj:TTHA0092.
    PATRICi23955115. VBITheThe93045_0090.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U52917 Genomic DNA. Translation: AAA97946.1 .
    AP008226 Genomic DNA. Translation: BAD69915.1 .
    PIRi T11906.
    RefSeqi YP_143358.1. NC_006461.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2FUG X-ray 3.30 9/G/P/Y 1-182 [» ]
    2YBB electron microscopy 19.00 8 1-182 [» ]
    3I9V X-ray 3.10 9/G 1-182 [» ]
    3IAM X-ray 3.10 9/G 1-182 [» ]
    3IAS X-ray 3.15 9/G/P/Y 1-182 [» ]
    3M9S X-ray 4.50 9/G 1-182 [» ]
    4HEA X-ray 3.30 9/O 1-182 [» ]
    ProteinModelPortali Q56224.
    SMRi Q56224. Positions 26-179.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-59267N.
    STRINGi 300852.TTHA0092.

    Protein family/group databases

    TCDBi 3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD69915 ; BAD69915 ; BAD69915 .
    GeneIDi 3167944.
    KEGGi ttj:TTHA0092.
    PATRICi 23955115. VBITheThe93045_0090.

    Phylogenomic databases

    eggNOGi COG1143.
    HOGENOMi HOG000228291.
    KOi K00338.
    OMAi EFELANY.
    OrthoDBi EOG6XM7H5.
    PhylomeDBi Q56224.

    Enzyme and pathway databases

    BioCyci TTHE300852:GH8R-96-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q56224.

    Family and domain databases

    HAMAPi MF_01351. NDH1_NuoI.
    InterProi IPR001450. 4Fe4S-bd_dom.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR010226. NADH_quinone_OxRdtase_chainI.
    [Graphical view ]
    Pfami PF00037. Fer4. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR01971. NuoI. 1 hit.
    PROSITEi PS00198. 4FE4S_FER_1. 2 hits.
    PS51379. 4FE4S_FER_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit."
      Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T.
      J. Biol. Chem. 272:4201-4211(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete genome sequence of Thermus thermophilus HB8."
      Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HB8 / ATCC 27634 / DSM 579.
    3. "Identification of a novel subunit of respiratory complex I from Thermus thermophilus."
      Hinchliffe P., Carroll J., Sazanov L.A.
      Biochemistry 45:4413-4420(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT.
    4. "Structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus."
      Sazanov L.A., Hinchliffe P.
      Science 311:1430-1436(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS), SUBUNIT, ELECTRON TRANSFER MECHANISM.

    Entry informationi

    Entry nameiNQO9_THET8
    AccessioniPrimary (citable) accession number: Q56224
    Secondary accession number(s): Q5SM51
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3