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Protein

NADH-quinone oxidoreductase subunit 9

Gene

nqo9

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. The role of the Nqo9 subunit appears to provide a 'connecting chain' of two clusters between cluster N5 and the terminal cluster N2, and to stabilize the structure of the complex by interacting with other subunits.2 Publications

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.

Cofactori

[4Fe-4S] cluster1 PublicationNote: Binds 2 [4Fe-4S] clusters per subunit. The [4Fe-4S] clusters are referred to as N6a and N6b.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi53Iron-sulfur 1 (4Fe-4S)1 Publication1
Metal bindingi56Iron-sulfur 1 (4Fe-4S)1 Publication1
Metal bindingi57Iron-sulfur (4Fe-4S) 1; via amide nitrogen1 Publication1
Metal bindingi59Iron-sulfur 1 (4Fe-4S)1 Publication1
Metal bindingi63Iron-sulfur 2 (4Fe-4S)1 Publication1
Metal bindingi98Iron-sulfur 2 (4Fe-4S)1 Publication1
Metal bindingi99Iron-sulfur (4Fe-4S) 2; via amide nitrogen1 Publication1
Metal bindingi101Iron-sulfur 2 (4Fe-4S)1 Publication1
Metal bindingi104Iron-sulfur 2 (4Fe-4S)1 Publication1
Metal bindingi108Iron-sulfur 1 (4Fe-4S)1 Publication1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD

Protein family/group databases

TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit 9 (EC:1.6.5.11)
Alternative name(s):
NADH dehydrogenase I subunit 9
NDH-1 subunit 9
Gene namesi
Name:nqo9
Ordered Locus Names:TTHA0092
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

  • Cell membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001187211 – 182NADH-quinone oxidoreductase subunit 9Add BLAST182

Keywords - PTMi

Quinone

Interactioni

Subunit structurei

NDH-1 is composed of 15 different subunits, Nqo1 to Nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits Nqo7, Nqo8 and Nqo10 to Nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits Nqo1 to Nqo6, Nqo9 and Nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.2 Publications

Protein-protein interaction databases

DIPiDIP-59267N.
STRINGi300852.TTHA0092.

Structurei

Secondary structure

1182
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 18Combined sources15
Turni26 – 28Combined sources3
Beta strandi40 – 43Combined sources4
Helixi58 – 62Combined sources5
Beta strandi68 – 73Combined sources6
Beta strandi77 – 79Combined sources3
Beta strandi81 – 85Combined sources5
Beta strandi87 – 94Combined sources8
Turni95 – 97Combined sources3
Helixi103 – 107Combined sources5
Beta strandi109 – 111Combined sources3
Beta strandi113 – 115Combined sources3
Beta strandi123 – 125Combined sources3
Helixi126 – 129Combined sources4
Beta strandi130 – 132Combined sources3
Helixi133 – 136Combined sources4
Helixi144 – 149Combined sources6
Turni150 – 153Combined sources4
Beta strandi160 – 162Combined sources3
Helixi168 – 170Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FUGX-ray3.309/G/P/Y1-182[»]
2YBBelectron microscopy19.0081-182[»]
3I9VX-ray3.109/G1-182[»]
3IAMX-ray3.109/G1-182[»]
3IASX-ray3.159/G/P/Y1-182[»]
3M9SX-ray4.509/G1-182[»]
4HEAX-ray3.309/O1-182[»]
ProteinModelPortaliQ56224.
SMRiQ56224.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ56224.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 734Fe-4S ferredoxin-type 1Add BLAST31
Domaini89 – 1184Fe-4S ferredoxin-type 2Add BLAST30

Sequence similaritiesi

Belongs to the complex I 23 kDa subunit family.Curated
Contains 2 4Fe-4S ferredoxin-type domains.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105P3U. Bacteria.
COG1143. LUCA.
HOGENOMiHOG000228291.
KOiK00338.
OMAiENYPDGP.
PhylomeDBiQ56224.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI. 1 hit.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q56224-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLKALAQSL GITLKYLFSK PVTVPYPDAP VALKPRFHGR HVLTRHPNGL
60 70 80 90 100
EKCIGCSLCA AACPAYAIYV EPAENDPENP VSAGERYAKV YEINMLRCIF
110 120 130 140 150
CGLCEEACPT GAIVLGYDFE MADYEYSDLV YGKEDMLVDV VGTKPQRREA
160 170 180
KRTGKPVKVG YVVPYVRPEL EGFKAPTEGG KR
Length:182
Mass (Da):20,080
Last modified:November 1, 1997 - v1
Checksum:i054A9F7942C64C66
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52917 Genomic DNA. Translation: AAA97946.1.
AP008226 Genomic DNA. Translation: BAD69915.1.
PIRiT11906.
RefSeqiWP_011174264.1. NC_006461.1.
YP_143358.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD69915; BAD69915; BAD69915.
GeneIDi3167944.
KEGGittj:TTHA0092.
PATRICi23955115. VBITheThe93045_0090.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52917 Genomic DNA. Translation: AAA97946.1.
AP008226 Genomic DNA. Translation: BAD69915.1.
PIRiT11906.
RefSeqiWP_011174264.1. NC_006461.1.
YP_143358.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FUGX-ray3.309/G/P/Y1-182[»]
2YBBelectron microscopy19.0081-182[»]
3I9VX-ray3.109/G1-182[»]
3IAMX-ray3.109/G1-182[»]
3IASX-ray3.159/G/P/Y1-182[»]
3M9SX-ray4.509/G1-182[»]
4HEAX-ray3.309/O1-182[»]
ProteinModelPortaliQ56224.
SMRiQ56224.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59267N.
STRINGi300852.TTHA0092.

Protein family/group databases

TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD69915; BAD69915; BAD69915.
GeneIDi3167944.
KEGGittj:TTHA0092.
PATRICi23955115. VBITheThe93045_0090.

Phylogenomic databases

eggNOGiENOG4105P3U. Bacteria.
COG1143. LUCA.
HOGENOMiHOG000228291.
KOiK00338.
OMAiENYPDGP.
PhylomeDBiQ56224.

Miscellaneous databases

EvolutionaryTraceiQ56224.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI. 1 hit.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNQO9_THET8
AccessioniPrimary (citable) accession number: Q56224
Secondary accession number(s): Q5SM51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.