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Q56224 (NQO9_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH-quinone oxidoreductase subunit 9

EC=1.6.99.5
Alternative name(s):
NADH dehydrogenase I subunit 9
NDH-1 subunit 9
Gene names
Name:nqo9
Ordered Locus Names:TTHA0092
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length182 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. The role of the nqo9 subunit appears to provide a 'connecting chain' of two clusters between cluster N5 and the terminal cluster N2, and to stabilize the structure of the complex by interacting with other subunits. HAMAP-Rule MF_01351

Catalytic activity

NADH + quinone = NAD+ + quinol. HAMAP-Rule MF_01351

Cofactor

Binds 2 4Fe-4S clusters per subunit. The 4Fe-4S clusters are referred to as N6a and N6b.

Subunit structure

NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers. Ref.3 Ref.4

Subcellular location

Cell membrane; Peripheral membrane protein; Cytoplasmic side HAMAP-Rule MF_01351.

Sequence similarities

Belongs to the complex I 23 kDa subunit family.

Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 182182NADH-quinone oxidoreductase subunit 9 HAMAP-Rule MF_01351
PRO_0000118721

Regions

Domain43 – 73314Fe-4S ferredoxin-type 1
Domain89 – 118304Fe-4S ferredoxin-type 2

Sites

Metal binding531Iron-sulfur 1 (4Fe-4S)
Metal binding561Iron-sulfur 1 (4Fe-4S)
Metal binding591Iron-sulfur 1 (4Fe-4S)
Metal binding631Iron-sulfur 2 (4Fe-4S)
Metal binding981Iron-sulfur 2 (4Fe-4S)
Metal binding1011Iron-sulfur 2 (4Fe-4S)
Metal binding1041Iron-sulfur 2 (4Fe-4S)
Metal binding1081Iron-sulfur 1 (4Fe-4S)

Secondary structure

.................................... 182
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q56224 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 054A9F7942C64C66

FASTA18220,080
        10         20         30         40         50         60 
MTLKALAQSL GITLKYLFSK PVTVPYPDAP VALKPRFHGR HVLTRHPNGL EKCIGCSLCA 

        70         80         90        100        110        120 
AACPAYAIYV EPAENDPENP VSAGERYAKV YEINMLRCIF CGLCEEACPT GAIVLGYDFE 

       130        140        150        160        170        180 
MADYEYSDLV YGKEDMLVDV VGTKPQRREA KRTGKPVKVG YVVPYVRPEL EGFKAPTEGG 


KR 

« Hide

References

« Hide 'large scale' references
[1]"The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit."
Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T.
J. Biol. Chem. 272:4201-4211(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.
[3]"Identification of a novel subunit of respiratory complex I from Thermus thermophilus."
Hinchliffe P., Carroll J., Sazanov L.A.
Biochemistry 45:4413-4420(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT.
[4]"Structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus."
Sazanov L.A., Hinchliffe P.
Science 311:1430-1436(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS), SUBUNIT, ELECTRON TRANSFER MECHANISM.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U52917 Genomic DNA. Translation: AAA97946.1.
AP008226 Genomic DNA. Translation: BAD69915.1.
PIRT11906.
RefSeqYP_143358.1. NC_006461.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2FUGX-ray3.309/G/P/Y1-182[»]
2YBBelectron microscopy19.0081-182[»]
3I9VX-ray3.109/G1-182[»]
3IAMX-ray3.109/G1-182[»]
3IASX-ray3.159/G/P/Y1-182[»]
3M9SX-ray4.509/G1-182[»]
4HEAX-ray3.309/O1-182[»]
ProteinModelPortalQ56224.
SMRQ56224. Positions 26-179.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-59267N.
STRING300852.TTHA0092.

Protein family/group databases

TCDB3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD69915; BAD69915; BAD69915.
GeneID3167944.
KEGGttj:TTHA0092.
PATRIC23955115. VBITheThe93045_0090.

Phylogenomic databases

eggNOGCOG1143.
HOGENOMHOG000228291.
KOK00338.
OMAVDCIALQ.
OrthoDBEOG6XM7H5.
ProtClustDBCLSK445099.

Enzyme and pathway databases

BioCycTTHE300852:GH8R-96-MONOMER.

Family and domain databases

HAMAPMF_01351. NDH1_NuoI.
InterProIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamPF00037. Fer4. 2 hits.
[Graphical view]
TIGRFAMsTIGR01971. NuoI. 1 hit.
PROSITEPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ56224.

Entry information

Entry nameNQO9_THET8
AccessionPrimary (citable) accession number: Q56224
Secondary accession number(s): Q5SM51
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: February 19, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references