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Protein

NADH-quinone oxidoreductase subunit 1

Gene

nqo1

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. The Nqo1 subunit contains the NADH-binding site and the primary electron acceptor FMN.2 Publications

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.

Cofactori

Protein has several cofactor binding sites:
  • FMN1 PublicationNote: Binds 1 FMN per subunit.1 Publication
  • [4Fe-4S] cluster1 PublicationNote: Binds 1 [4Fe-4S] cluster per subunit. This [4Fe-4S] cluster is referred to as N3.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei75FMN1 Publication1
Binding sitei97NADCurated1
Metal bindingi353Iron-sulfur (4Fe-4S)1 Publication1
Metal bindingi356Iron-sulfur (4Fe-4S)1 Publication1
Metal bindingi359Iron-sulfur (4Fe-4S)1 Publication1
Metal bindingi400Iron-sulfur (4Fe-4S)1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi66 – 69NADCurated4
Nucleotide bindingi92 – 96FMN1 Publication5
Nucleotide bindingi180 – 185FMN1 Publication6
Nucleotide bindingi218 – 220FMN1 Publication3

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, Flavoprotein, FMN, Iron, Iron-sulfur, Metal-binding, NAD

Protein family/group databases

TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit 1 (EC:1.6.5.11)
Alternative name(s):
NADH dehydrogenase I chain 1
NDH-1 subunit 1
Gene namesi
Name:nqo1
Ordered Locus Names:TTHA0089
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

  • Cell membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001185671 – 438NADH-quinone oxidoreductase subunit 1Add BLAST438

Keywords - PTMi

Quinone

Interactioni

Subunit structurei

NDH-1 is composed of 15 different subunits, Nqo1 to Nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits Nqo7, Nqo8 and Nqo10 to Nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits Nqo1 to Nqo6, Nqo9 and Nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.2 Publications

Protein-protein interaction databases

DIPiDIP-59259N.
STRINGi300852.TTHA0089.

Structurei

Secondary structure

1438
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 6Combined sources3
Helixi18 – 20Combined sources3
Turni24 – 27Combined sources4
Helixi29 – 34Combined sources6
Turni35 – 38Combined sources4
Helixi39 – 46Combined sources8
Helixi50 – 58Combined sources9
Turni59 – 61Combined sources3
Turni65 – 68Combined sources4
Helixi72 – 76Combined sources5
Beta strandi81 – 83Combined sources3
Beta strandi88 – 92Combined sources5
Helixi103 – 109Combined sources7
Helixi111 – 125Combined sources15
Beta strandi129 – 134Combined sources6
Helixi139 – 154Combined sources16
Beta strandi157 – 159Combined sources3
Helixi162 – 164Combined sources3
Beta strandi169 – 175Combined sources7
Helixi180 – 183Combined sources4
Helixi185 – 192Combined sources8
Beta strandi202 – 204Combined sources3
Turni206 – 208Combined sources3
Helixi211 – 213Combined sources3
Beta strandi216 – 220Combined sources5
Helixi221 – 225Combined sources5
Helixi227 – 232Combined sources6
Helixi235 – 239Combined sources5
Beta strandi240 – 242Combined sources3
Beta strandi244 – 260Combined sources17
Beta strandi262 – 267Combined sources6
Helixi272 – 277Combined sources6
Turni278 – 280Combined sources3
Beta strandi283 – 285Combined sources3
Beta strandi287 – 291Combined sources5
Beta strandi293 – 297Combined sources5
Beta strandi300 – 302Combined sources3
Helixi304 – 307Combined sources4
Turni313 – 316Combined sources4
Helixi317 – 319Combined sources3
Beta strandi324 – 332Combined sources9
Helixi337 – 351Combined sources15
Helixi357 – 360Combined sources4
Helixi361 – 365Combined sources5
Helixi366 – 374Combined sources9
Turni375 – 377Combined sources3
Helixi381 – 394Combined sources14
Helixi402 – 426Combined sources25

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FUGX-ray3.301/A/J/S1-438[»]
2YBBelectron microscopy19.0011-438[»]
3I9VX-ray3.101/A1-438[»]
3IAMX-ray3.101/A1-438[»]
3IASX-ray3.151/A/J/S1-438[»]
3M9SX-ray4.501/A1-438[»]
4HEAX-ray3.301/B1-438[»]
ProteinModelPortaliQ56222.
SMRiQ56222.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ56222.

Family & Domainsi

Domaini

The subunit can be separated roughly into four domains: an N-terminal domain (residues 7 to 72) ending with a glycine-rich loop, followed by a Rossman-fold domain (73 to 240), a ubiquitin-like domain (241 to 335) and a C-terminal four-helical bundle containing cluster N3 (336 to 438).1 Publication

Sequence similaritiesi

Belongs to the complex I 51 kDa subunit family.Curated

Phylogenomic databases

eggNOGiENOG4107EEP. Bacteria.
COG1894. LUCA.
HOGENOMiHOG000251534.
KOiK00335.
OMAiPEGVPRY.
PhylomeDBiQ56222.

Family and domain databases

InterProiIPR001949. NADH-UbQ_OxRdtase_51kDa_CS.
IPR011537. NADH-UbQ_OxRdtase_suF.
IPR011538. Nuo51_FMN-bd.
IPR019575. Nuop51_4Fe4S-bd.
IPR019554. Soluble_ligand-bd.
[Graphical view]
PfamiPF01512. Complex1_51K. 1 hit.
PF10589. NADH_4Fe-4S. 1 hit.
PF10531. SLBB. 1 hit.
[Graphical view]
SMARTiSM00928. NADH_4Fe-4S. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01959. nuoF_fam. 1 hit.
PROSITEiPS00644. COMPLEX1_51K_1. 1 hit.
PS00645. COMPLEX1_51K_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q56222-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGPILSGLD PRFERTLYAH VGKEGSWTLD YYLRHGGYET AKRVLKEKTP
60 70 80 90 100
DEVIEEVKRS GLRGRGGAGF PTGLKWSFMP KDDGKQHYLI CNADESEPGS
110 120 130 140 150
FKDRYILEDV PHLLIEGMIL AGYAIRATVG YIYVRGEYRR AADRLEQAIK
160 170 180 190 200
EARARGYLGK NLFGTDFSFD LHVHRGAGAY ICGEETALMN SLEGLRANPR
210 220 230 240 250
LKPPFPAQSG LWGKPTTINN VETLASVVPI MERGADWFAQ MGTEQSKGMK
260 270 280 290 300
LYQISGPVKR PGVYELPMGT TFRELIYEWA GGPLEPIQAI IPGGSSTPPL
310 320 330 340 350
PFTEEVLDTP MSYEHLQAKG SMLGTGGVIL IPERVSMVDA MWNLTRFYAH
360 370 380 390 400
ESCGKCTPCR EGVAGFMVNL FAKIGTGQGE EKDVENLEAL LPLIEGRSFC
410 420 430
PLADAAVWPV KGSLRHFKDQ YLALAREKRP VPRPSLWR
Length:438
Mass (Da):48,632
Last modified:November 1, 1997 - v1
Checksum:i73ADB3AF31BBE4C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52917 Genomic DNA. Translation: AAA97943.1.
AP008226 Genomic DNA. Translation: BAD69912.1.
PIRiT11903.
RefSeqiWP_011227700.1. NC_006461.1.
YP_143355.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD69912; BAD69912; BAD69912.
GeneIDi3168453.
KEGGittj:TTHA0089.
PATRICi23955109. VBITheThe93045_0087.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52917 Genomic DNA. Translation: AAA97943.1.
AP008226 Genomic DNA. Translation: BAD69912.1.
PIRiT11903.
RefSeqiWP_011227700.1. NC_006461.1.
YP_143355.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FUGX-ray3.301/A/J/S1-438[»]
2YBBelectron microscopy19.0011-438[»]
3I9VX-ray3.101/A1-438[»]
3IAMX-ray3.101/A1-438[»]
3IASX-ray3.151/A/J/S1-438[»]
3M9SX-ray4.501/A1-438[»]
4HEAX-ray3.301/B1-438[»]
ProteinModelPortaliQ56222.
SMRiQ56222.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59259N.
STRINGi300852.TTHA0089.

Protein family/group databases

TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD69912; BAD69912; BAD69912.
GeneIDi3168453.
KEGGittj:TTHA0089.
PATRICi23955109. VBITheThe93045_0087.

Phylogenomic databases

eggNOGiENOG4107EEP. Bacteria.
COG1894. LUCA.
HOGENOMiHOG000251534.
KOiK00335.
OMAiPEGVPRY.
PhylomeDBiQ56222.

Miscellaneous databases

EvolutionaryTraceiQ56222.

Family and domain databases

InterProiIPR001949. NADH-UbQ_OxRdtase_51kDa_CS.
IPR011537. NADH-UbQ_OxRdtase_suF.
IPR011538. Nuo51_FMN-bd.
IPR019575. Nuop51_4Fe4S-bd.
IPR019554. Soluble_ligand-bd.
[Graphical view]
PfamiPF01512. Complex1_51K. 1 hit.
PF10589. NADH_4Fe-4S. 1 hit.
PF10531. SLBB. 1 hit.
[Graphical view]
SMARTiSM00928. NADH_4Fe-4S. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01959. nuoF_fam. 1 hit.
PROSITEiPS00644. COMPLEX1_51K_1. 1 hit.
PS00645. COMPLEX1_51K_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNQO1_THET8
AccessioniPrimary (citable) accession number: Q56222
Secondary accession number(s): Q5SM54
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.