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Q56221

- NQO2_THET8

UniProt

Q56221 - NQO2_THET8

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Protein

NADH-quinone oxidoreductase subunit 2

Gene

nqo2

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.

Catalytic activityi

NADH + quinone = NAD+ + quinol.

Cofactori

[2Fe-2S] clusterNote: Binds 1 [2Fe-2S] cluster. This [2Fe-2S] cluster is referred to as N1a.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi83 – 831Iron-sulfur (2Fe-2S)
Metal bindingi88 – 881Iron-sulfur (2Fe-2S)
Metal bindingi124 – 1241Iron-sulfur (2Fe-2S)
Metal bindingi128 – 1281Iron-sulfur (2Fe-2S)

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. NADH dehydrogenase (quinone) activity Source: UniProtKB-EC
  4. quinone binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding, NAD

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-92-MONOMER.

Protein family/group databases

TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit 2 (EC:1.6.99.5)
Alternative name(s):
NADH dehydrogenase I chain 2
NDH-1 subunit 2
Gene namesi
Name:nqo2
Ordered Locus Names:TTHA0088
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
ProteomesiUP000000532: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 181180NADH-quinone oxidoreductase subunit 2PRO_0000118686Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi144 ↔ 172

Keywords - PTMi

Disulfide bond, Quinone

Proteomic databases

PRIDEiQ56221.

Interactioni

Subunit structurei

NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.2 Publications

Protein-protein interaction databases

DIPiDIP-59260N.
STRINGi300852.TTHA0088.

Structurei

Secondary structure

1
181
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni4 – 63Combined sources
Helixi8 – 158Combined sources
Helixi24 – 263Combined sources
Helixi27 – 3812Combined sources
Helixi43 – 5311Combined sources
Helixi57 – 648Combined sources
Beta strandi67 – 704Combined sources
Beta strandi77 – 837Combined sources
Helixi86 – 894Combined sources
Turni90 – 923Combined sources
Helixi93 – 10412Combined sources
Beta strandi117 – 1237Combined sources
Helixi128 – 1303Combined sources
Beta strandi132 – 1354Combined sources
Beta strandi137 – 1393Combined sources
Beta strandi142 – 1443Combined sources
Helixi147 – 15812Combined sources
Helixi163 – 1653Combined sources
Beta strandi174 – 1763Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FUGX-ray3.302/B/K/T1-181[»]
2YBBelectron microscopy19.0021-181[»]
3I9VX-ray3.102/B1-181[»]
3IAMX-ray3.102/B1-181[»]
3IASX-ray3.152/B/K/T1-181[»]
3M9SX-ray4.502/B1-181[»]
4HEAX-ray3.302/C1-181[»]
ProteinModelPortaliQ56221.
SMRiQ56221. Positions 3-180.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ56221.

Family & Domainsi

Domaini

The subunit can be divided into two domains: an N-terminal four-helical bundle (residues 2 to 74) involved in interactions with subunits nqo1 and nqo3, and a thioredoxin-like C-terminal domain (residues 75 to 180) that coordinates the binuclear cluster N1a.

Sequence similaritiesi

Belongs to the complex I 24 kDa subunit family.Curated

Phylogenomic databases

eggNOGiCOG1905.
HOGENOMiHOG000257748.
KOiK00334.
OMAiHIPHIRV.
OrthoDBiEOG689HX9.
PhylomeDBiQ56221.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR002023. NuoE-like.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR10371. PTHR10371. 1 hit.
PIRSFiPIRSF000216. NADH_DH_24kDa. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR01958. nuoE_fam. 1 hit.
PROSITEiPS01099. COMPLEX1_24K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q56221-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGFFDDKQDF LEETFAKYPP EGRRAAIMPL LRRVQQEEGW IRPERIEEIA
60 70 80 90 100
RLVGTTPTEV MGVASFYSYY QFVPTGKYHL QVCATLSCKL AGAEELWDYL
110 120 130 140 150
TETLGIGPGE VTPDGLFSVQ KVECLGSCHT APVIQVNDEP YVECVTRARL
160 170 180
EALLAGLRAG KRLEEIELPG KCGHHVHEVE V
Length:181
Mass (Da):20,286
Last modified:January 23, 2007 - v3
Checksum:i484FE09245C613EE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52917 Genomic DNA. Translation: AAA97942.1.
AP008226 Genomic DNA. Translation: BAD69911.1.
PIRiT11902.
RefSeqiYP_143354.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD69911; BAD69911; BAD69911.
GeneIDi3168326.
KEGGittj:TTHA0088.
PATRICi23955107. VBITheThe93045_0086.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52917 Genomic DNA. Translation: AAA97942.1 .
AP008226 Genomic DNA. Translation: BAD69911.1 .
PIRi T11902.
RefSeqi YP_143354.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2FUG X-ray 3.30 2/B/K/T 1-181 [» ]
2YBB electron microscopy 19.00 2 1-181 [» ]
3I9V X-ray 3.10 2/B 1-181 [» ]
3IAM X-ray 3.10 2/B 1-181 [» ]
3IAS X-ray 3.15 2/B/K/T 1-181 [» ]
3M9S X-ray 4.50 2/B 1-181 [» ]
4HEA X-ray 3.30 2/C 1-181 [» ]
ProteinModelPortali Q56221.
SMRi Q56221. Positions 3-180.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-59260N.
STRINGi 300852.TTHA0088.

Protein family/group databases

TCDBi 3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PRIDEi Q56221.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD69911 ; BAD69911 ; BAD69911 .
GeneIDi 3168326.
KEGGi ttj:TTHA0088.
PATRICi 23955107. VBITheThe93045_0086.

Phylogenomic databases

eggNOGi COG1905.
HOGENOMi HOG000257748.
KOi K00334.
OMAi HIPHIRV.
OrthoDBi EOG689HX9.
PhylomeDBi Q56221.

Enzyme and pathway databases

BioCyci TTHE300852:GH8R-92-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q56221.

Family and domain databases

Gene3Di 3.40.30.10. 1 hit.
InterProi IPR002023. NuoE-like.
IPR012336. Thioredoxin-like_fold.
[Graphical view ]
PANTHERi PTHR10371. PTHR10371. 1 hit.
PIRSFi PIRSF000216. NADH_DH_24kDa. 1 hit.
SUPFAMi SSF52833. SSF52833. 1 hit.
TIGRFAMsi TIGR01958. nuoE_fam. 1 hit.
PROSITEi PS01099. COMPLEX1_24K. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit."
    Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T.
    J. Biol. Chem. 272:4201-4211(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
  2. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.
  3. "Identification of a novel subunit of respiratory complex I from Thermus thermophilus."
    Hinchliffe P., Carroll J., Sazanov L.A.
    Biochemistry 45:4413-4420(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-9, IDENTIFICATION BY MASS SPECTROMETRY, EPR SPECTROSCOPY, SUBUNIT.
  4. "Structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus."
    Sazanov L.A., Hinchliffe P.
    Science 311:1430-1436(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS), SUBUNIT, ELECTRON TRANSFER MECHANISM.

Entry informationi

Entry nameiNQO2_THET8
AccessioniPrimary (citable) accession number: Q56221
Secondary accession number(s): Q5SM55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 116 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3