Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NADH-quinone oxidoreductase subunit 2

Gene

nqo2

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.2 Publications

Cofactori

[2Fe-2S] cluster1 PublicationNote: Binds 1 [2Fe-2S] cluster. This [2Fe-2S] cluster is referred to as N1a.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi83Iron-sulfur (2Fe-2S)1 Publication1
Metal bindingi87Iron-sulfur (2Fe-2S)1 Publication1
Metal bindingi88Iron-sulfur (2Fe-2S)1 Publication1
Metal bindingi124Iron-sulfur (2Fe-2S)1 Publication1
Metal bindingi128Iron-sulfur (2Fe-2S)1 Publication1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding, NAD

Protein family/group databases

TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit 2 (EC:1.6.5.11)
Alternative name(s):
NADH dehydrogenase I chain 2
NDH-1 subunit 2
Gene namesi
Name:nqo2
Ordered Locus Names:TTHA0088
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

  • Cell membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001186862 – 181NADH-quinone oxidoreductase subunit 2Add BLAST180

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi144 ↔ 1721 Publication

Keywords - PTMi

Disulfide bond, Quinone

Proteomic databases

PRIDEiQ56221.

Interactioni

Subunit structurei

NDH-1 is composed of 15 different subunits, Nqo1 to Nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits Nqo7, Nqo8 and Nqo10 to Nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits Nqo1 to Nqo6, Nqo9 and Nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.2 Publications

Protein-protein interaction databases

DIPiDIP-59260N.
STRINGi300852.TTHA0088.

Structurei

Secondary structure

1181
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni4 – 6Combined sources3
Helixi8 – 15Combined sources8
Helixi24 – 26Combined sources3
Helixi27 – 38Combined sources12
Helixi43 – 53Combined sources11
Helixi57 – 64Combined sources8
Beta strandi67 – 70Combined sources4
Beta strandi77 – 83Combined sources7
Helixi86 – 89Combined sources4
Turni90 – 92Combined sources3
Helixi93 – 104Combined sources12
Beta strandi117 – 123Combined sources7
Helixi128 – 130Combined sources3
Beta strandi132 – 135Combined sources4
Beta strandi137 – 139Combined sources3
Beta strandi142 – 144Combined sources3
Helixi147 – 158Combined sources12
Helixi163 – 165Combined sources3
Beta strandi174 – 176Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FUGX-ray3.302/B/K/T1-181[»]
2YBBelectron microscopy19.0021-181[»]
3I9VX-ray3.102/B1-181[»]
3IAMX-ray3.102/B1-181[»]
3IASX-ray3.152/B/K/T1-181[»]
3M9SX-ray4.502/B1-181[»]
4HEAX-ray3.302/C1-181[»]
ProteinModelPortaliQ56221.
SMRiQ56221.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ56221.

Family & Domainsi

Domaini

The subunit can be divided into two domains: an N-terminal four-helical bundle (residues 2 to 74) involved in interactions with subunits Nqo1 and Nqo3, and a thioredoxin-like C-terminal domain (residues 75 to 180) that coordinates the binuclear cluster N1a.1 Publication

Sequence similaritiesi

Belongs to the complex I 24 kDa subunit family.Curated

Phylogenomic databases

eggNOGiENOG4105GKE. Bacteria.
COG1905. LUCA.
HOGENOMiHOG000257748.
KOiK00334.
OMAiCDKGPSM.
PhylomeDBiQ56221.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR002023. NuoE-like.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PIRSFiPIRSF000216. NADH_DH_24kDa. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR01958. nuoE_fam. 1 hit.
PROSITEiPS01099. COMPLEX1_24K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q56221-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGFFDDKQDF LEETFAKYPP EGRRAAIMPL LRRVQQEEGW IRPERIEEIA
60 70 80 90 100
RLVGTTPTEV MGVASFYSYY QFVPTGKYHL QVCATLSCKL AGAEELWDYL
110 120 130 140 150
TETLGIGPGE VTPDGLFSVQ KVECLGSCHT APVIQVNDEP YVECVTRARL
160 170 180
EALLAGLRAG KRLEEIELPG KCGHHVHEVE V
Length:181
Mass (Da):20,286
Last modified:January 23, 2007 - v3
Checksum:i484FE09245C613EE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52917 Genomic DNA. Translation: AAA97942.1.
AP008226 Genomic DNA. Translation: BAD69911.1.
PIRiT11902.
RefSeqiWP_011174268.1. NC_006461.1.
YP_143354.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD69911; BAD69911; BAD69911.
GeneIDi3168326.
KEGGittj:TTHA0088.
PATRICi23955107. VBITheThe93045_0086.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52917 Genomic DNA. Translation: AAA97942.1.
AP008226 Genomic DNA. Translation: BAD69911.1.
PIRiT11902.
RefSeqiWP_011174268.1. NC_006461.1.
YP_143354.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FUGX-ray3.302/B/K/T1-181[»]
2YBBelectron microscopy19.0021-181[»]
3I9VX-ray3.102/B1-181[»]
3IAMX-ray3.102/B1-181[»]
3IASX-ray3.152/B/K/T1-181[»]
3M9SX-ray4.502/B1-181[»]
4HEAX-ray3.302/C1-181[»]
ProteinModelPortaliQ56221.
SMRiQ56221.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59260N.
STRINGi300852.TTHA0088.

Protein family/group databases

TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PRIDEiQ56221.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD69911; BAD69911; BAD69911.
GeneIDi3168326.
KEGGittj:TTHA0088.
PATRICi23955107. VBITheThe93045_0086.

Phylogenomic databases

eggNOGiENOG4105GKE. Bacteria.
COG1905. LUCA.
HOGENOMiHOG000257748.
KOiK00334.
OMAiCDKGPSM.
PhylomeDBiQ56221.

Miscellaneous databases

EvolutionaryTraceiQ56221.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR002023. NuoE-like.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PIRSFiPIRSF000216. NADH_DH_24kDa. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR01958. nuoE_fam. 1 hit.
PROSITEiPS01099. COMPLEX1_24K. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNQO2_THET8
AccessioniPrimary (citable) accession number: Q56221
Secondary accession number(s): Q5SM55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.