Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q56221 (NQO2_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH-quinone oxidoreductase subunit 2

EC=1.6.99.5
Alternative name(s):
NADH dehydrogenase I chain 2
NDH-1 subunit 2
Gene names
Name:nqo2
Ordered Locus Names:TTHA0088
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length181 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.

Catalytic activity

NADH + quinone = NAD+ + quinol.

Cofactor

Binds 1 2Fe-2S cluster. This 2Fe-2S cluster is referred to as N1a.

Subunit structure

NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers. Ref.3 Ref.4

Subcellular location

Cell membrane; Peripheral membrane protein; Cytoplasmic side.

Domain

The subunit can be divided into two domains: an N-terminal four-helical bundle (residues 2 to 74) involved in interactions with subunits nqo1 and nqo3, and a thioredoxin-like C-terminal domain (residues 75 to 180) that coordinates the binuclear cluster N1a.

Sequence similarities

Belongs to the complex I 24 kDa subunit family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.3
Chain2 – 181180NADH-quinone oxidoreductase subunit 2
PRO_0000118686

Sites

Metal binding831Iron-sulfur (2Fe-2S)
Metal binding881Iron-sulfur (2Fe-2S)
Metal binding1241Iron-sulfur (2Fe-2S)
Metal binding1281Iron-sulfur (2Fe-2S)

Amino acid modifications

Disulfide bond144 ↔ 172

Secondary structure

.................................... 181
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q56221 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 484FE09245C613EE

FASTA18120,286
        10         20         30         40         50         60 
MGFFDDKQDF LEETFAKYPP EGRRAAIMPL LRRVQQEEGW IRPERIEEIA RLVGTTPTEV 

        70         80         90        100        110        120 
MGVASFYSYY QFVPTGKYHL QVCATLSCKL AGAEELWDYL TETLGIGPGE VTPDGLFSVQ 

       130        140        150        160        170        180 
KVECLGSCHT APVIQVNDEP YVECVTRARL EALLAGLRAG KRLEEIELPG KCGHHVHEVE 


V 

« Hide

References

« Hide 'large scale' references
[1]"The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit."
Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T.
J. Biol. Chem. 272:4201-4211(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
[2]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.
[3]"Identification of a novel subunit of respiratory complex I from Thermus thermophilus."
Hinchliffe P., Carroll J., Sazanov L.A.
Biochemistry 45:4413-4420(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-9, IDENTIFICATION BY MASS SPECTROMETRY, EPR SPECTROSCOPY, SUBUNIT.
[4]"Structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus."
Sazanov L.A., Hinchliffe P.
Science 311:1430-1436(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS), SUBUNIT, ELECTRON TRANSFER MECHANISM.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U52917 Genomic DNA. Translation: AAA97942.1.
AP008226 Genomic DNA. Translation: BAD69911.1.
PIRT11902.
RefSeqYP_143354.1. NC_006461.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2FUGX-ray3.302/B/K/T1-181[»]
2YBBelectron microscopy19.0021-181[»]
3I9VX-ray3.102/B1-181[»]
3IAMX-ray3.102/B1-181[»]
3IASX-ray3.152/B/K/T1-181[»]
3M9SX-ray4.502/B1-181[»]
4HEAX-ray3.302/C1-181[»]
ProteinModelPortalQ56221.
SMRQ56221. Positions 3-180.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-59260N.
STRING300852.TTHA0088.

Protein family/group databases

TCDB3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PRIDEQ56221.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD69911; BAD69911; BAD69911.
GeneID3168326.
KEGGttj:TTHA0088.
PATRIC23955107. VBITheThe93045_0086.

Phylogenomic databases

eggNOGCOG1905.
HOGENOMHOG000257748.
KOK00334.
OMAHIPHIRV.
OrthoDBEOG689HX9.
PhylomeDBQ56221.

Enzyme and pathway databases

BioCycTTHE300852:GH8R-92-MONOMER.

Family and domain databases

Gene3D3.40.30.10. 1 hit.
InterProIPR002023. NuoE-like.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERPTHR10371. PTHR10371. 1 hit.
PIRSFPIRSF000216. NADH_DH_24kDa. 1 hit.
SUPFAMSSF52833. SSF52833. 1 hit.
TIGRFAMsTIGR01958. nuoE_fam. 1 hit.
PROSITEPS01099. COMPLEX1_24K. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ56221.

Entry information

Entry nameNQO2_THET8
AccessionPrimary (citable) accession number: Q56221
Secondary accession number(s): Q5SM55
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references