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Q56221

- NQO2_THET8

UniProt

Q56221 - NQO2_THET8

Protein

NADH-quinone oxidoreductase subunit 2

Gene

nqo2

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.

    Catalytic activityi

    NADH + quinone = NAD+ + quinol.

    Cofactori

    Binds 1 2Fe-2S cluster. This 2Fe-2S cluster is referred to as N1a.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi83 – 831Iron-sulfur (2Fe-2S)
    Metal bindingi88 – 881Iron-sulfur (2Fe-2S)
    Metal bindingi124 – 1241Iron-sulfur (2Fe-2S)
    Metal bindingi128 – 1281Iron-sulfur (2Fe-2S)

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. NADH dehydrogenase (quinone) activity Source: UniProtKB-EC
    4. quinone binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    2Fe-2S, Iron, Iron-sulfur, Metal-binding, NAD

    Enzyme and pathway databases

    BioCyciTTHE300852:GH8R-92-MONOMER.

    Protein family/group databases

    TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH-quinone oxidoreductase subunit 2 (EC:1.6.99.5)
    Alternative name(s):
    NADH dehydrogenase I chain 2
    NDH-1 subunit 2
    Gene namesi
    Name:nqo2
    Ordered Locus Names:TTHA0088
    OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
    Taxonomic identifieri300852 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
    ProteomesiUP000000532: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 181180NADH-quinone oxidoreductase subunit 2PRO_0000118686Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi144 ↔ 172

    Keywords - PTMi

    Disulfide bond, Quinone

    Proteomic databases

    PRIDEiQ56221.

    Interactioni

    Subunit structurei

    NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.2 Publications

    Protein-protein interaction databases

    DIPiDIP-59260N.
    STRINGi300852.TTHA0088.

    Structurei

    Secondary structure

    1
    181
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni4 – 63
    Helixi8 – 158
    Helixi24 – 263
    Helixi27 – 3812
    Helixi43 – 5311
    Helixi57 – 648
    Beta strandi67 – 704
    Beta strandi77 – 837
    Helixi86 – 894
    Turni90 – 923
    Helixi93 – 10412
    Beta strandi117 – 1237
    Helixi128 – 1303
    Beta strandi132 – 1354
    Beta strandi137 – 1393
    Beta strandi142 – 1443
    Helixi147 – 15812
    Helixi163 – 1653
    Beta strandi174 – 1763

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2FUGX-ray3.302/B/K/T1-181[»]
    2YBBelectron microscopy19.0021-181[»]
    3I9VX-ray3.102/B1-181[»]
    3IAMX-ray3.102/B1-181[»]
    3IASX-ray3.152/B/K/T1-181[»]
    3M9SX-ray4.502/B1-181[»]
    4HEAX-ray3.302/C1-181[»]
    ProteinModelPortaliQ56221.
    SMRiQ56221. Positions 3-180.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ56221.

    Family & Domainsi

    Domaini

    The subunit can be divided into two domains: an N-terminal four-helical bundle (residues 2 to 74) involved in interactions with subunits nqo1 and nqo3, and a thioredoxin-like C-terminal domain (residues 75 to 180) that coordinates the binuclear cluster N1a.

    Sequence similaritiesi

    Belongs to the complex I 24 kDa subunit family.Curated

    Phylogenomic databases

    eggNOGiCOG1905.
    HOGENOMiHOG000257748.
    KOiK00334.
    OMAiHIPHIRV.
    OrthoDBiEOG689HX9.
    PhylomeDBiQ56221.

    Family and domain databases

    Gene3Di3.40.30.10. 1 hit.
    InterProiIPR002023. NuoE-like.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PANTHERiPTHR10371. PTHR10371. 1 hit.
    PIRSFiPIRSF000216. NADH_DH_24kDa. 1 hit.
    SUPFAMiSSF52833. SSF52833. 1 hit.
    TIGRFAMsiTIGR01958. nuoE_fam. 1 hit.
    PROSITEiPS01099. COMPLEX1_24K. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q56221-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGFFDDKQDF LEETFAKYPP EGRRAAIMPL LRRVQQEEGW IRPERIEEIA    50
    RLVGTTPTEV MGVASFYSYY QFVPTGKYHL QVCATLSCKL AGAEELWDYL 100
    TETLGIGPGE VTPDGLFSVQ KVECLGSCHT APVIQVNDEP YVECVTRARL 150
    EALLAGLRAG KRLEEIELPG KCGHHVHEVE V 181
    Length:181
    Mass (Da):20,286
    Last modified:January 23, 2007 - v3
    Checksum:i484FE09245C613EE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U52917 Genomic DNA. Translation: AAA97942.1.
    AP008226 Genomic DNA. Translation: BAD69911.1.
    PIRiT11902.
    RefSeqiYP_143354.1. NC_006461.1.

    Genome annotation databases

    EnsemblBacteriaiBAD69911; BAD69911; BAD69911.
    GeneIDi3168326.
    KEGGittj:TTHA0088.
    PATRICi23955107. VBITheThe93045_0086.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U52917 Genomic DNA. Translation: AAA97942.1 .
    AP008226 Genomic DNA. Translation: BAD69911.1 .
    PIRi T11902.
    RefSeqi YP_143354.1. NC_006461.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2FUG X-ray 3.30 2/B/K/T 1-181 [» ]
    2YBB electron microscopy 19.00 2 1-181 [» ]
    3I9V X-ray 3.10 2/B 1-181 [» ]
    3IAM X-ray 3.10 2/B 1-181 [» ]
    3IAS X-ray 3.15 2/B/K/T 1-181 [» ]
    3M9S X-ray 4.50 2/B 1-181 [» ]
    4HEA X-ray 3.30 2/C 1-181 [» ]
    ProteinModelPortali Q56221.
    SMRi Q56221. Positions 3-180.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-59260N.
    STRINGi 300852.TTHA0088.

    Protein family/group databases

    TCDBi 3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Proteomic databases

    PRIDEi Q56221.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD69911 ; BAD69911 ; BAD69911 .
    GeneIDi 3168326.
    KEGGi ttj:TTHA0088.
    PATRICi 23955107. VBITheThe93045_0086.

    Phylogenomic databases

    eggNOGi COG1905.
    HOGENOMi HOG000257748.
    KOi K00334.
    OMAi HIPHIRV.
    OrthoDBi EOG689HX9.
    PhylomeDBi Q56221.

    Enzyme and pathway databases

    BioCyci TTHE300852:GH8R-92-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q56221.

    Family and domain databases

    Gene3Di 3.40.30.10. 1 hit.
    InterProi IPR002023. NuoE-like.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    PANTHERi PTHR10371. PTHR10371. 1 hit.
    PIRSFi PIRSF000216. NADH_DH_24kDa. 1 hit.
    SUPFAMi SSF52833. SSF52833. 1 hit.
    TIGRFAMsi TIGR01958. nuoE_fam. 1 hit.
    PROSITEi PS01099. COMPLEX1_24K. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit."
      Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T.
      J. Biol. Chem. 272:4201-4211(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    2. "Complete genome sequence of Thermus thermophilus HB8."
      Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HB8 / ATCC 27634 / DSM 579.
    3. "Identification of a novel subunit of respiratory complex I from Thermus thermophilus."
      Hinchliffe P., Carroll J., Sazanov L.A.
      Biochemistry 45:4413-4420(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-9, IDENTIFICATION BY MASS SPECTROMETRY, EPR SPECTROSCOPY, SUBUNIT.
    4. "Structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus."
      Sazanov L.A., Hinchliffe P.
      Science 311:1430-1436(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS), SUBUNIT, ELECTRON TRANSFER MECHANISM.

    Entry informationi

    Entry nameiNQO2_THET8
    AccessioniPrimary (citable) accession number: Q56221
    Secondary accession number(s): Q5SM55
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 114 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3