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Q56220

- NQO4_THET8

UniProt

Q56220 - NQO4_THET8

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Protein

NADH-quinone oxidoreductase subunit 4

Gene

nqo4

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. The nqo4 subunit may contain the quinone-binding site.

Catalytic activityi

NADH + quinone = NAD+ + quinol.

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. NADH dehydrogenase (quinone) activity Source: UniProtKB-HAMAP
  3. quinone binding Source: UniProtKB-KW

GO - Biological processi

  1. transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Transport

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-91-MONOMER.

Protein family/group databases

TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit 4 (EC:1.6.99.5)
Alternative name(s):
NADH dehydrogenase I chain 4
NDH-1 subunit 4
Gene namesi
Name:nqo4
Ordered Locus Names:TTHA0087
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
ProteomesiUP000000532: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 409409NADH-quinone oxidoreductase subunit 4PRO_0000118617Add
BLAST

Keywords - PTMi

Quinone

Interactioni

Subunit structurei

NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers. Subunit nqo4 interacts extensively with nqo6.2 Publications

Protein-protein interaction databases

DIPiDIP-59262N.
STRINGi300852.TTHA0087.

Structurei

Secondary structure

1
409
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi28 – 303Combined sources
Beta strandi36 – 383Combined sources
Beta strandi42 – 465Combined sources
Beta strandi48 – 514Combined sources
Beta strandi53 – 586Combined sources
Helixi66 – 694Combined sources
Helixi70 – 723Combined sources
Helixi77 – 804Combined sources
Helixi82 – 843Combined sources
Beta strandi85 – 895Combined sources
Helixi91 – 10515Combined sources
Helixi111 – 13929Combined sources
Helixi144 – 16421Combined sources
Beta strandi165 – 1695Combined sources
Beta strandi175 – 1806Combined sources
Helixi186 – 20924Combined sources
Helixi214 – 2185Combined sources
Beta strandi219 – 2224Combined sources
Helixi226 – 2327Combined sources
Beta strandi236 – 2394Combined sources
Helixi240 – 2423Combined sources
Helixi248 – 2514Combined sources
Helixi257 – 2593Combined sources
Beta strandi268 – 2703Combined sources
Helixi272 – 29524Combined sources
Turni307 – 3093Combined sources
Helixi314 – 3185Combined sources
Helixi321 – 33212Combined sources
Beta strandi339 – 3435Combined sources
Beta strandi346 – 3483Combined sources
Beta strandi351 – 3599Combined sources
Beta strandi361 – 3644Combined sources
Beta strandi366 – 3716Combined sources
Helixi373 – 3764Combined sources
Turni377 – 3859Combined sources
Beta strandi386 – 3883Combined sources
Helixi391 – 3933Combined sources
Helixi395 – 3984Combined sources
Helixi404 – 4085Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FUGX-ray3.304/D/M/V1-409[»]
2YBBelectron microscopy19.0041-409[»]
3I9VX-ray3.104/D1-409[»]
3IAMX-ray3.104/D1-409[»]
3IASX-ray3.154/D/M/V1-409[»]
3M9SX-ray4.504/D1-409[»]
4HEAX-ray3.304/E1-409[»]
ProteinModelPortaliQ56220.
SMRiQ56220. Positions 35-409.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ56220.

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 49 kDa subunit family.Curated

Phylogenomic databases

eggNOGiCOG0649.
HOGENOMiHOG000228264.
KOiK00333.
OMAiQCFALAV.
OrthoDBiEOG62G5MP.
PhylomeDBiQ56220.

Family and domain databases

Gene3Di1.10.645.10. 1 hit.
HAMAPiMF_01358. NDH1_NuoD.
InterProiIPR001135. NADH_Q_OxRdtase_suD.
IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
IPR022885. NDH1_su_D/H.
IPR029014. NiFe_Hase-like.
[Graphical view]
PfamiPF00346. Complex1_49kDa. 1 hit.
[Graphical view]
SUPFAMiSSF56762. SSF56762. 1 hit.
TIGRFAMsiTIGR01962. NuoD. 1 hit.
PROSITEiPS00535. COMPLEX1_49K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q56220-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MREEFLEEIP LDAPPEEAKE LRTEVMTLNV GPQHPSTHGV LRLMVTLSGE
60 70 80 90 100
EVLEVVPHIG YLHTGFEKTM EHRTYLQNIT YTPRMDYLHS FAHDLAYALA
110 120 130 140 150
VEKLLGAVVP PRAETIRVIL NELSRLASHL VFLGTGLLDL GALTPFFYAF
160 170 180 190 200
RERETILDLF EWVTGQRFHH NYIRIGGVKE DLPEEFVPEL KKLLEVLPHR
210 220 230 240 250
IDEYEALFAE SPIFYERARG VGVIPPEVAI DLGLTGGSLR ASGVNYDVRK
260 270 280 290 300
AYPYSGYETY TFDVPLGERG DVFDRMLVRI REMRESVKII KQALERLEPG
310 320 330 340 350
PVRDPNPQIT PPPRHLLETS MEAVIYHFKH YTEGFHPPKG EVYVPTESAR
360 370 380 390 400
GELGYYIVSD GGSMPYRVKV RAPSFVNLQS LPYACKGEQV PDMVAIIASL

DPVMGDVDR
Length:409
Mass (Da):46,371
Last modified:November 1, 1997 - v1
Checksum:i161AA0C796D62ED3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52917 Genomic DNA. Translation: AAA97941.1.
AP008226 Genomic DNA. Translation: BAD69910.1.
PIRiT11901.
RefSeqiWP_011227699.1. NC_006461.1.
YP_143353.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD69910; BAD69910; BAD69910.
GeneIDi3168206.
KEGGittj:TTHA0087.
PATRICi23955105. VBITheThe93045_0085.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52917 Genomic DNA. Translation: AAA97941.1 .
AP008226 Genomic DNA. Translation: BAD69910.1 .
PIRi T11901.
RefSeqi WP_011227699.1. NC_006461.1.
YP_143353.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2FUG X-ray 3.30 4/D/M/V 1-409 [» ]
2YBB electron microscopy 19.00 4 1-409 [» ]
3I9V X-ray 3.10 4/D 1-409 [» ]
3IAM X-ray 3.10 4/D 1-409 [» ]
3IAS X-ray 3.15 4/D/M/V 1-409 [» ]
3M9S X-ray 4.50 4/D 1-409 [» ]
4HEA X-ray 3.30 4/E 1-409 [» ]
ProteinModelPortali Q56220.
SMRi Q56220. Positions 35-409.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-59262N.
STRINGi 300852.TTHA0087.

Protein family/group databases

TCDBi 3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD69910 ; BAD69910 ; BAD69910 .
GeneIDi 3168206.
KEGGi ttj:TTHA0087.
PATRICi 23955105. VBITheThe93045_0085.

Phylogenomic databases

eggNOGi COG0649.
HOGENOMi HOG000228264.
KOi K00333.
OMAi QCFALAV.
OrthoDBi EOG62G5MP.
PhylomeDBi Q56220.

Enzyme and pathway databases

BioCyci TTHE300852:GH8R-91-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q56220.

Family and domain databases

Gene3Di 1.10.645.10. 1 hit.
HAMAPi MF_01358. NDH1_NuoD.
InterProi IPR001135. NADH_Q_OxRdtase_suD.
IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
IPR022885. NDH1_su_D/H.
IPR029014. NiFe_Hase-like.
[Graphical view ]
Pfami PF00346. Complex1_49kDa. 1 hit.
[Graphical view ]
SUPFAMi SSF56762. SSF56762. 1 hit.
TIGRFAMsi TIGR01962. NuoD. 1 hit.
PROSITEi PS00535. COMPLEX1_49K. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit."
    Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T.
    J. Biol. Chem. 272:4201-4211(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.
  3. "Identification of a novel subunit of respiratory complex I from Thermus thermophilus."
    Hinchliffe P., Carroll J., Sazanov L.A.
    Biochemistry 45:4413-4420(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-8, IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT.
  4. "Structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus."
    Sazanov L.A., Hinchliffe P.
    Science 311:1430-1436(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS), SUBUNIT, ELECTRON TRANSFER MECHANISM.

Entry informationi

Entry nameiNQO4_THET8
AccessioniPrimary (citable) accession number: Q56220
Secondary accession number(s): Q5SM56
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 26, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3