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Q56220

- NQO4_THET8

UniProt

Q56220 - NQO4_THET8

Protein

NADH-quinone oxidoreductase subunit 4

Gene

nqo4

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. The nqo4 subunit may contain the quinone-binding site.

    Catalytic activityi

    NADH + quinone = NAD+ + quinol.

    GO - Molecular functioni

    1. NAD binding Source: InterPro
    2. NADH dehydrogenase (quinone) activity Source: UniProtKB-HAMAP
    3. quinone binding Source: UniProtKB-KW

    GO - Biological processi

    1. transport Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Transport

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciTTHE300852:GH8R-91-MONOMER.

    Protein family/group databases

    TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH-quinone oxidoreductase subunit 4 (EC:1.6.99.5)
    Alternative name(s):
    NADH dehydrogenase I chain 4
    NDH-1 subunit 4
    Gene namesi
    Name:nqo4
    Ordered Locus Names:TTHA0087
    OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
    Taxonomic identifieri300852 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
    ProteomesiUP000000532: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 409409NADH-quinone oxidoreductase subunit 4PRO_0000118617Add
    BLAST

    Keywords - PTMi

    Quinone

    Interactioni

    Subunit structurei

    NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers. Subunit nqo4 interacts extensively with nqo6.2 Publications

    Protein-protein interaction databases

    DIPiDIP-59262N.
    STRINGi300852.TTHA0087.

    Structurei

    Secondary structure

    1
    409
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi28 – 303
    Beta strandi36 – 383
    Beta strandi42 – 465
    Beta strandi48 – 514
    Beta strandi53 – 586
    Helixi66 – 694
    Helixi70 – 723
    Helixi77 – 804
    Helixi82 – 843
    Beta strandi85 – 895
    Helixi91 – 10515
    Helixi111 – 13929
    Helixi144 – 16421
    Beta strandi165 – 1695
    Beta strandi175 – 1806
    Helixi186 – 20924
    Helixi214 – 2185
    Beta strandi219 – 2224
    Helixi226 – 2327
    Beta strandi236 – 2394
    Helixi240 – 2423
    Helixi248 – 2514
    Helixi257 – 2593
    Beta strandi268 – 2703
    Helixi272 – 29524
    Turni307 – 3093
    Helixi314 – 3185
    Helixi321 – 33212
    Beta strandi339 – 3435
    Beta strandi346 – 3483
    Beta strandi351 – 3599
    Beta strandi361 – 3644
    Beta strandi366 – 3716
    Helixi373 – 3764
    Turni377 – 3859
    Beta strandi386 – 3883
    Helixi391 – 3933
    Helixi395 – 3984
    Helixi404 – 4085

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2FUGX-ray3.304/D/M/V1-409[»]
    2YBBelectron microscopy19.0041-409[»]
    3I9VX-ray3.104/D1-409[»]
    3IAMX-ray3.104/D1-409[»]
    3IASX-ray3.154/D/M/V1-409[»]
    3M9SX-ray4.504/D1-409[»]
    4HEAX-ray3.304/E1-409[»]
    ProteinModelPortaliQ56220.
    SMRiQ56220. Positions 35-409.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ56220.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the complex I 49 kDa subunit family.Curated

    Phylogenomic databases

    eggNOGiCOG0649.
    HOGENOMiHOG000228264.
    KOiK00333.
    OMAiQCFALAV.
    OrthoDBiEOG62G5MP.
    PhylomeDBiQ56220.

    Family and domain databases

    Gene3Di1.10.645.10. 1 hit.
    HAMAPiMF_01358. NDH1_NuoD.
    InterProiIPR001135. NADH_Q_OxRdtase_suD.
    IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
    IPR022885. NDH1_su_D/H.
    IPR029014. NiFe_Hase-like.
    [Graphical view]
    PfamiPF00346. Complex1_49kDa. 1 hit.
    [Graphical view]
    SUPFAMiSSF56762. SSF56762. 1 hit.
    TIGRFAMsiTIGR01962. NuoD. 1 hit.
    PROSITEiPS00535. COMPLEX1_49K. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q56220-1 [UniParc]FASTAAdd to Basket

    « Hide

    MREEFLEEIP LDAPPEEAKE LRTEVMTLNV GPQHPSTHGV LRLMVTLSGE    50
    EVLEVVPHIG YLHTGFEKTM EHRTYLQNIT YTPRMDYLHS FAHDLAYALA 100
    VEKLLGAVVP PRAETIRVIL NELSRLASHL VFLGTGLLDL GALTPFFYAF 150
    RERETILDLF EWVTGQRFHH NYIRIGGVKE DLPEEFVPEL KKLLEVLPHR 200
    IDEYEALFAE SPIFYERARG VGVIPPEVAI DLGLTGGSLR ASGVNYDVRK 250
    AYPYSGYETY TFDVPLGERG DVFDRMLVRI REMRESVKII KQALERLEPG 300
    PVRDPNPQIT PPPRHLLETS MEAVIYHFKH YTEGFHPPKG EVYVPTESAR 350
    GELGYYIVSD GGSMPYRVKV RAPSFVNLQS LPYACKGEQV PDMVAIIASL 400
    DPVMGDVDR 409
    Length:409
    Mass (Da):46,371
    Last modified:November 1, 1997 - v1
    Checksum:i161AA0C796D62ED3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U52917 Genomic DNA. Translation: AAA97941.1.
    AP008226 Genomic DNA. Translation: BAD69910.1.
    PIRiT11901.
    RefSeqiWP_011227699.1. NC_006461.1.
    YP_143353.1. NC_006461.1.

    Genome annotation databases

    EnsemblBacteriaiBAD69910; BAD69910; BAD69910.
    GeneIDi3168206.
    KEGGittj:TTHA0087.
    PATRICi23955105. VBITheThe93045_0085.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U52917 Genomic DNA. Translation: AAA97941.1 .
    AP008226 Genomic DNA. Translation: BAD69910.1 .
    PIRi T11901.
    RefSeqi WP_011227699.1. NC_006461.1.
    YP_143353.1. NC_006461.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2FUG X-ray 3.30 4/D/M/V 1-409 [» ]
    2YBB electron microscopy 19.00 4 1-409 [» ]
    3I9V X-ray 3.10 4/D 1-409 [» ]
    3IAM X-ray 3.10 4/D 1-409 [» ]
    3IAS X-ray 3.15 4/D/M/V 1-409 [» ]
    3M9S X-ray 4.50 4/D 1-409 [» ]
    4HEA X-ray 3.30 4/E 1-409 [» ]
    ProteinModelPortali Q56220.
    SMRi Q56220. Positions 35-409.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-59262N.
    STRINGi 300852.TTHA0087.

    Protein family/group databases

    TCDBi 3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD69910 ; BAD69910 ; BAD69910 .
    GeneIDi 3168206.
    KEGGi ttj:TTHA0087.
    PATRICi 23955105. VBITheThe93045_0085.

    Phylogenomic databases

    eggNOGi COG0649.
    HOGENOMi HOG000228264.
    KOi K00333.
    OMAi QCFALAV.
    OrthoDBi EOG62G5MP.
    PhylomeDBi Q56220.

    Enzyme and pathway databases

    BioCyci TTHE300852:GH8R-91-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q56220.

    Family and domain databases

    Gene3Di 1.10.645.10. 1 hit.
    HAMAPi MF_01358. NDH1_NuoD.
    InterProi IPR001135. NADH_Q_OxRdtase_suD.
    IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
    IPR022885. NDH1_su_D/H.
    IPR029014. NiFe_Hase-like.
    [Graphical view ]
    Pfami PF00346. Complex1_49kDa. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56762. SSF56762. 1 hit.
    TIGRFAMsi TIGR01962. NuoD. 1 hit.
    PROSITEi PS00535. COMPLEX1_49K. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit."
      Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T.
      J. Biol. Chem. 272:4201-4211(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete genome sequence of Thermus thermophilus HB8."
      Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HB8 / ATCC 27634 / DSM 579.
    3. "Identification of a novel subunit of respiratory complex I from Thermus thermophilus."
      Hinchliffe P., Carroll J., Sazanov L.A.
      Biochemistry 45:4413-4420(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-8, IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT.
    4. "Structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus."
      Sazanov L.A., Hinchliffe P.
      Science 311:1430-1436(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS), SUBUNIT, ELECTRON TRANSFER MECHANISM.

    Entry informationi

    Entry nameiNQO4_THET8
    AccessioniPrimary (citable) accession number: Q56220
    Secondary accession number(s): Q5SM56
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3