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Protein

NADH-quinone oxidoreductase subunit 4

Gene

nqo4

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. The Nqo4 subunit may contain the quinone-binding site.2 Publications

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
Biological processTransport
LigandNAD

Enzyme and pathway databases

BioCyciTTHE300852:G1GKC-90-MONOMER

Protein family/group databases

TCDBi3.D.1.3.1 the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit 4 (EC:1.6.5.11)
Alternative name(s):
NADH dehydrogenase I chain 4
NDH-1 subunit 4
Gene namesi
Name:nqo4
Ordered Locus Names:TTHA0087
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

  • Cell membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001186171 – 409NADH-quinone oxidoreductase subunit 4Add BLAST409

Keywords - PTMi

Quinone

Interactioni

Subunit structurei

NDH-1 is composed of 15 different subunits, Nqo1 to Nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits Nqo7, Nqo8 and Nqo10 to Nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits Nqo1 to Nqo6, Nqo9 and Nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers. This subunit interacts extensively with Nqo6.2 Publications

Protein-protein interaction databases

DIPiDIP-59262N
IntActiQ56220, 1 interactor
STRINGi300852.TTHA0087

Structurei

Secondary structure

1409
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi28 – 30Combined sources3
Beta strandi36 – 38Combined sources3
Beta strandi42 – 46Combined sources5
Beta strandi48 – 51Combined sources4
Beta strandi53 – 58Combined sources6
Helixi66 – 69Combined sources4
Helixi70 – 72Combined sources3
Helixi77 – 80Combined sources4
Helixi82 – 84Combined sources3
Beta strandi85 – 89Combined sources5
Helixi91 – 105Combined sources15
Helixi111 – 139Combined sources29
Helixi144 – 164Combined sources21
Beta strandi165 – 169Combined sources5
Beta strandi175 – 180Combined sources6
Helixi186 – 209Combined sources24
Helixi214 – 218Combined sources5
Beta strandi219 – 222Combined sources4
Helixi226 – 232Combined sources7
Beta strandi236 – 239Combined sources4
Helixi240 – 242Combined sources3
Helixi248 – 251Combined sources4
Helixi257 – 259Combined sources3
Beta strandi268 – 270Combined sources3
Helixi272 – 295Combined sources24
Turni307 – 309Combined sources3
Helixi314 – 318Combined sources5
Helixi321 – 332Combined sources12
Beta strandi339 – 343Combined sources5
Beta strandi346 – 348Combined sources3
Beta strandi351 – 359Combined sources9
Beta strandi361 – 364Combined sources4
Beta strandi366 – 371Combined sources6
Helixi373 – 376Combined sources4
Turni377 – 385Combined sources9
Beta strandi386 – 388Combined sources3
Helixi391 – 393Combined sources3
Helixi395 – 398Combined sources4
Helixi404 – 408Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FUGX-ray3.304/D/M/V1-409[»]
2YBBelectron microscopy19.0041-409[»]
3I9VX-ray3.104/D1-409[»]
3IAMX-ray3.104/D1-409[»]
3IASX-ray3.154/D/M/V1-409[»]
3M9SX-ray4.504/D1-409[»]
4HEAX-ray3.304/E1-409[»]
ProteinModelPortaliQ56220
SMRiQ56220
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ56220

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 49 kDa subunit family.Curated

Phylogenomic databases

eggNOGiENOG4105CQV Bacteria
COG0649 LUCA
HOGENOMiHOG000228264
KOiK00333
OMAiKKIAWPA
PhylomeDBiQ56220

Family and domain databases

Gene3Di1.10.645.20, 1 hit
HAMAPiMF_01358 NDH1_NuoD, 1 hit
InterProiView protein in InterPro
IPR001135 NADH_Q_OxRdtase_suD
IPR038290 NADH_Q_OxRdtase_suD_sf
IPR014029 NADH_UbQ_OxRdtase_49kDa_CS
IPR022885 NDH1_su_D/H
IPR029014 NiFe-Hase_large
PfamiView protein in Pfam
PF00346 Complex1_49kDa, 1 hit
SUPFAMiSSF56762 SSF56762, 1 hit
TIGRFAMsiTIGR01962 NuoD, 1 hit
PROSITEiView protein in PROSITE
PS00535 COMPLEX1_49K, 1 hit

Sequencei

Sequence statusi: Complete.

Q56220-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREEFLEEIP LDAPPEEAKE LRTEVMTLNV GPQHPSTHGV LRLMVTLSGE
60 70 80 90 100
EVLEVVPHIG YLHTGFEKTM EHRTYLQNIT YTPRMDYLHS FAHDLAYALA
110 120 130 140 150
VEKLLGAVVP PRAETIRVIL NELSRLASHL VFLGTGLLDL GALTPFFYAF
160 170 180 190 200
RERETILDLF EWVTGQRFHH NYIRIGGVKE DLPEEFVPEL KKLLEVLPHR
210 220 230 240 250
IDEYEALFAE SPIFYERARG VGVIPPEVAI DLGLTGGSLR ASGVNYDVRK
260 270 280 290 300
AYPYSGYETY TFDVPLGERG DVFDRMLVRI REMRESVKII KQALERLEPG
310 320 330 340 350
PVRDPNPQIT PPPRHLLETS MEAVIYHFKH YTEGFHPPKG EVYVPTESAR
360 370 380 390 400
GELGYYIVSD GGSMPYRVKV RAPSFVNLQS LPYACKGEQV PDMVAIIASL

DPVMGDVDR
Length:409
Mass (Da):46,371
Last modified:November 1, 1997 - v1
Checksum:i161AA0C796D62ED3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52917 Genomic DNA Translation: AAA97941.1
AP008226 Genomic DNA Translation: BAD69910.1
PIRiT11901
RefSeqiWP_011227699.1, NC_006461.1
YP_143353.1, NC_006461.1

Genome annotation databases

EnsemblBacteriaiBAD69910; BAD69910; BAD69910
GeneIDi3168206
KEGGittj:TTHA0087
PATRICifig|300852.9.peg.85

Similar proteinsi

Entry informationi

Entry nameiNQO4_THET8
AccessioniPrimary (citable) accession number: Q56220
Secondary accession number(s): Q5SM56
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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