Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q56220 (NQO4_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH-quinone oxidoreductase subunit 4

EC=1.6.99.5
Alternative name(s):
NADH dehydrogenase I chain 4
NDH-1 subunit 4
Gene names
Name:nqo4
Ordered Locus Names:TTHA0087
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length409 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. The nqo4 subunit may contain the quinone-binding site. HAMAP-Rule MF_01358

Catalytic activity

NADH + quinone = NAD+ + quinol. HAMAP-Rule MF_01358

Subunit structure

NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers. Subunit nqo4 interacts extensively with nqo6. Ref.3 Ref.4

Subcellular location

Cell membrane; Peripheral membrane protein; Cytoplasmic side HAMAP-Rule MF_01358.

Sequence similarities

Belongs to the complex I 49 kDa subunit family.

Ontologies

Keywords
   Biological processTransport
   Cellular componentCell membrane
Membrane
   LigandNAD
   Molecular functionOxidoreductase
   PTMQuinone
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processtransport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionNAD binding

Inferred from electronic annotation. Source: InterPro

NADH dehydrogenase (quinone) activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

quinone binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 409409NADH-quinone oxidoreductase subunit 4 HAMAP-Rule MF_01358
PRO_0000118617

Secondary structure

........................................................................ 409
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q56220 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 161AA0C796D62ED3

FASTA40946,371
        10         20         30         40         50         60 
MREEFLEEIP LDAPPEEAKE LRTEVMTLNV GPQHPSTHGV LRLMVTLSGE EVLEVVPHIG 

        70         80         90        100        110        120 
YLHTGFEKTM EHRTYLQNIT YTPRMDYLHS FAHDLAYALA VEKLLGAVVP PRAETIRVIL 

       130        140        150        160        170        180 
NELSRLASHL VFLGTGLLDL GALTPFFYAF RERETILDLF EWVTGQRFHH NYIRIGGVKE 

       190        200        210        220        230        240 
DLPEEFVPEL KKLLEVLPHR IDEYEALFAE SPIFYERARG VGVIPPEVAI DLGLTGGSLR 

       250        260        270        280        290        300 
ASGVNYDVRK AYPYSGYETY TFDVPLGERG DVFDRMLVRI REMRESVKII KQALERLEPG 

       310        320        330        340        350        360 
PVRDPNPQIT PPPRHLLETS MEAVIYHFKH YTEGFHPPKG EVYVPTESAR GELGYYIVSD 

       370        380        390        400 
GGSMPYRVKV RAPSFVNLQS LPYACKGEQV PDMVAIIASL DPVMGDVDR 

« Hide

References

« Hide 'large scale' references
[1]"The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit."
Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T.
J. Biol. Chem. 272:4201-4211(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.
[3]"Identification of a novel subunit of respiratory complex I from Thermus thermophilus."
Hinchliffe P., Carroll J., Sazanov L.A.
Biochemistry 45:4413-4420(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-8, IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT.
[4]"Structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus."
Sazanov L.A., Hinchliffe P.
Science 311:1430-1436(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS), SUBUNIT, ELECTRON TRANSFER MECHANISM.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U52917 Genomic DNA. Translation: AAA97941.1.
AP008226 Genomic DNA. Translation: BAD69910.1.
PIRT11901.
RefSeqYP_143353.1. NC_006461.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2FUGX-ray3.304/D/M/V1-409[»]
2YBBelectron microscopy19.0041-409[»]
3I9VX-ray3.104/D1-409[»]
3IAMX-ray3.104/D1-409[»]
3IASX-ray3.154/D/M/V1-409[»]
3M9SX-ray4.504/D1-409[»]
4HEAX-ray3.304/E1-409[»]
ProteinModelPortalQ56220.
SMRQ56220. Positions 35-409.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-59262N.
STRING300852.TTHA0087.

Protein family/group databases

TCDB3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD69910; BAD69910; BAD69910.
GeneID3168206.
KEGGttj:TTHA0087.
PATRIC23955105. VBITheThe93045_0085.

Phylogenomic databases

eggNOGCOG0649.
HOGENOMHOG000228264.
KOK00333.
OMAEYRTWTQ.
OrthoDBEOG62G5MP.
ProtClustDBCLSK445104.

Enzyme and pathway databases

BioCycTTHE300852:GH8R-91-MONOMER.

Family and domain databases

HAMAPMF_01358. NDH1_NuoD.
InterProIPR001135. NADH_Q_OxRdtase_suD.
IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
IPR022885. NDH1_su_D/H.
[Graphical view]
PfamPF00346. Complex1_49kDa. 1 hit.
[Graphical view]
TIGRFAMsTIGR01962. NuoD. 1 hit.
PROSITEPS00535. COMPLEX1_49K. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ56220.

Entry information

Entry nameNQO4_THET8
AccessionPrimary (citable) accession number: Q56220
Secondary accession number(s): Q5SM56
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: February 19, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references