Reviewed, UniProtKB/Swiss-Prot Q56219 (NQO5_THET8)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit 5
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I chain 5
    NDH-1 subunit 5

Gene names

Name:	nqo5
Ordered Locus Names:	TTHA0086

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Complete proteome] [HAMAP]

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. The nqo5 subunit may be involved in the stabilization of the complex. HAMAP MF_01357
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01357
Subunit structure	NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers. The N-terminal domain of subunit nqo5 (residues 1 to 153) wraps around nqo4 on one side, and the subunit interacts also with nqo9 via a two-stranded beta sheet (residues 154 to 171) and with nqo3 via an extended C-terminal loop (residues 172 to 196). Ref.3 Ref.4
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side HAMAP MF_01357.
Sequence similarities	Belongs to the complex I 30 kDa subunit family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: HAMAP photosynthesis, light reaction Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 207

207

NADH-quinone oxidoreductase subunit 5 HAMAP MF_01357

PRO_0000118669

Secondary structure

1

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207

Helix Strand Turn

Details...

Helix

2 – 11

10

Turn

12 – 14

3

Beta strand

17 – 19

3

Beta strand

21 – 23

3

Beta strand

25 – 28

4

Helix

34 – 40

7

Turn

41 – 45

5

Beta strand

52 – 56

5

Beta strand

65 – 75

11

Beta strand

80 – 82

3

Beta strand

87 – 94

8

Helix

115 – 124

10

Beta strand

141 – 143

3

Beta strand

145 – 147

3

Helix

167 – 170

4

Sequences

	Sequence		Length	Mass (Da)
	Q56219-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: EB6891BEF6F314AD	FASTA	207	23,859
	10 20 30 40 50 60 MRLERVLEEA RAKGYPIEDN GLGNLWVVLP RERFKEEMAH YKAMGFNFLA DIVGLDYLTY 70 80 90 100 110 120 PDPRPERFAV VYELVSLPGW KDGDGSRFFV RVYVPEEDPR LPTVTDLWGS ANFLEREVYD 130 140 150 160 170 180 LFGIVFEGHP DLRKILTPED LEGHPLRKDY PLGETPTLFR EGRYIIPAEF RAALTGKDPG 190 200 LTFYKGGSRK GYRSLWADLK KAREVKG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit." Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T. J. Biol. Chem. 272:4201-4211(1997) [PubMed: 9020134] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Identification of a novel subunit of respiratory complex I from Thermus thermophilus." Hinchliffe P., Carroll J., Sazanov L.A. Biochemistry 45:4413-4420(2006) [PubMed: 16584177] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-8, IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT.
[4]	"Structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus." Sazanov L.A., Hinchliffe P. Science 311:1430-1436(2006) [PubMed: 16469879] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS), SUBUNIT, ELECTRON TRANSFER MECHANISM.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U52917 Genomic DNA. Translation: AAA97940.1.
AP008226 Genomic DNA. Translation: BAD69909.1.

PIR

T11900.

RefSeq

YP_143352.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2FUG	X-ray	3.30	5/E/N/W	1-207	[»]
3I9V	X-ray	3.10	5/E	1-207	[»]
3IAM	X-ray	3.10	5/E	1-207	[»]
3IAS	X-ray	3.15	5/E/N/W	1-207	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

Q56219.

Protein family/group databases

TCDB

3.D.1.3.1. H+ or Na+-translocating NADH dehydrogenase (NDH) family.

Genome annotation databases

GeneID

3168317.

GenomeReviews

Gene locus TTHA0086 in contig AP008226_GR.

KEGG

ttj:TTHA0086.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

COG0852.

HOGENOM

HBG244769.

OMA

ERECHEM.

Enzyme and pathway databases

BioCyc

TTHE300852:TTHA0086-MONOMER.

Family and domain databases

HAMAP

MF_01357. NDH1_NuoC.
[Tree]

InterPro

IPR010218. NADH_DH_csu.
IPR001268. NADH_UbQ_OxRdtase_30kDa_su.
IPR020396. NADH_UbQ_OxRdtase_CS.
[Graphical view]

Pfam

PF00329. Complex1_30kDa. 1 hit.
[Graphical view]

ProDom

PD001581. NADH_UbQ_OxRdtase_30kDa_su. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR01961. NuoC_fam. 1 hit.

PROSITE

PS00542. COMPLEX1_30K. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

NQO5_THET8

Accession

Primary (citable) accession number: Q56219
Secondary accession number(s): Q5SM57

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	February 9, 2010
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)