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Q56219

- NQO5_THET8

UniProt

Q56219 - NQO5_THET8

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Protein

NADH-quinone oxidoreductase subunit 5

Gene

nqo5

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. The nqo5 subunit may be involved in the stabilization of the complex.

Catalytic activityi

NADH + quinone = NAD+ + quinol.UniRule annotation

GO - Molecular functioni

  1. NADH dehydrogenase (ubiquinone) activity Source: InterPro
  2. quinone binding Source: UniProtKB-KW

GO - Biological processi

  1. transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Transport

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-90-MONOMER.

Protein family/group databases

TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit 5 (EC:1.6.99.5UniRule annotation)
Alternative name(s):
NADH dehydrogenase I chain 5
NDH-1 subunit 5
Gene namesi
Name:nqo5
Ordered Locus Names:TTHA0086
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
ProteomesiUP000000532: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 207207NADH-quinone oxidoreductase subunit 5PRO_0000118669Add
BLAST

Keywords - PTMi

Quinone

Interactioni

Subunit structurei

NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers. The N-terminal domain of subunit nqo5 (residues 1 to 153) wraps around nqo4 on one side, and the subunit interacts also with nqo9 via a two-stranded beta sheet (residues 154 to 171) and with nqo3 via an extended C-terminal loop (residues 172 to 196).2 Publications

Protein-protein interaction databases

DIPiDIP-59263N.
STRINGi300852.TTHA0086.

Structurei

Secondary structure

1
207
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 129
Beta strandi17 – 193
Beta strandi21 – 233
Beta strandi25 – 284
Helixi31 – 4313
Beta strandi49 – 568
Beta strandi65 – 7410
Beta strandi80 – 823
Beta strandi88 – 947
Beta strandi100 – 1023
Turni105 – 1073
Helixi111 – 12010
Beta strandi125 – 1273
Beta strandi135 – 1373
Beta strandi141 – 1433
Beta strandi145 – 1473
Beta strandi160 – 1623
Helixi167 – 1693
Beta strandi176 – 1783
Beta strandi181 – 1833
Helixi187 – 1893

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FUGX-ray3.305/E/N/W1-207[»]
2YBBelectron microscopy19.0051-207[»]
3I9VX-ray3.105/E1-207[»]
3IAMX-ray3.105/E1-207[»]
3IASX-ray3.155/E/N/W1-207[»]
3M9SX-ray4.505/E1-207[»]
4HEAX-ray3.305/F1-207[»]
ProteinModelPortaliQ56219.
SMRiQ56219. Positions 1-196.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ56219.

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 30 kDa subunit family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0852.
HOGENOMiHOG000099654.
KOiK00332.
OMAiYEERECH.
OrthoDBiEOG60PHFK.
PhylomeDBiQ56219.

Family and domain databases

HAMAPiMF_01357. NDH1_NuoC.
InterProiIPR010218. NADH_DH_suC.
IPR001268. NADH_UbQ_OxRdtase_30kDa_su.
IPR020396. NADH_UbQ_OxRdtase_CS.
[Graphical view]
PfamiPF00329. Complex1_30kDa. 1 hit.
[Graphical view]
ProDomiPD001581. NADH_UbQ_OxRdtase_30kDa_su. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS00542. COMPLEX1_30K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q56219-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRLERVLEEA RAKGYPIEDN GLGNLWVVLP RERFKEEMAH YKAMGFNFLA
60 70 80 90 100
DIVGLDYLTY PDPRPERFAV VYELVSLPGW KDGDGSRFFV RVYVPEEDPR
110 120 130 140 150
LPTVTDLWGS ANFLEREVYD LFGIVFEGHP DLRKILTPED LEGHPLRKDY
160 170 180 190 200
PLGETPTLFR EGRYIIPAEF RAALTGKDPG LTFYKGGSRK GYRSLWADLK

KAREVKG
Length:207
Mass (Da):23,859
Last modified:November 1, 1997 - v1
Checksum:iEB6891BEF6F314AD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U52917 Genomic DNA. Translation: AAA97940.1.
AP008226 Genomic DNA. Translation: BAD69909.1.
PIRiT11900.
RefSeqiYP_143352.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD69909; BAD69909; BAD69909.
GeneIDi3168317.
KEGGittj:TTHA0086.
PATRICi23955103. VBITheThe93045_0084.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U52917 Genomic DNA. Translation: AAA97940.1 .
AP008226 Genomic DNA. Translation: BAD69909.1 .
PIRi T11900.
RefSeqi YP_143352.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2FUG X-ray 3.30 5/E/N/W 1-207 [» ]
2YBB electron microscopy 19.00 5 1-207 [» ]
3I9V X-ray 3.10 5/E 1-207 [» ]
3IAM X-ray 3.10 5/E 1-207 [» ]
3IAS X-ray 3.15 5/E/N/W 1-207 [» ]
3M9S X-ray 4.50 5/E 1-207 [» ]
4HEA X-ray 3.30 5/F 1-207 [» ]
ProteinModelPortali Q56219.
SMRi Q56219. Positions 1-196.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-59263N.
STRINGi 300852.TTHA0086.

Protein family/group databases

TCDBi 3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD69909 ; BAD69909 ; BAD69909 .
GeneIDi 3168317.
KEGGi ttj:TTHA0086.
PATRICi 23955103. VBITheThe93045_0084.

Phylogenomic databases

eggNOGi COG0852.
HOGENOMi HOG000099654.
KOi K00332.
OMAi YEERECH.
OrthoDBi EOG60PHFK.
PhylomeDBi Q56219.

Enzyme and pathway databases

BioCyci TTHE300852:GH8R-90-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q56219.

Family and domain databases

HAMAPi MF_01357. NDH1_NuoC.
InterProi IPR010218. NADH_DH_suC.
IPR001268. NADH_UbQ_OxRdtase_30kDa_su.
IPR020396. NADH_UbQ_OxRdtase_CS.
[Graphical view ]
Pfami PF00329. Complex1_30kDa. 1 hit.
[Graphical view ]
ProDomi PD001581. NADH_UbQ_OxRdtase_30kDa_su. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
PROSITEi PS00542. COMPLEX1_30K. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit."
    Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T.
    J. Biol. Chem. 272:4201-4211(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.
  3. "Identification of a novel subunit of respiratory complex I from Thermus thermophilus."
    Hinchliffe P., Carroll J., Sazanov L.A.
    Biochemistry 45:4413-4420(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-8, IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT.
  4. "Structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus."
    Sazanov L.A., Hinchliffe P.
    Science 311:1430-1436(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS), SUBUNIT, ELECTRON TRANSFER MECHANISM.

Entry informationi

Entry nameiNQO5_THET8
AccessioniPrimary (citable) accession number: Q56219
Secondary accession number(s): Q5SM57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3