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Q56219 (NQO5_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH-quinone oxidoreductase subunit 5

EC=1.6.99.5
Alternative name(s):
NADH dehydrogenase I chain 5
NDH-1 subunit 5
Gene names
Name:nqo5
Ordered Locus Names:TTHA0086
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length207 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. The nqo5 subunit may be involved in the stabilization of the complex. HAMAP-Rule MF_01357

Catalytic activity

NADH + quinone = NAD+ + quinol. HAMAP-Rule MF_01357

Subunit structure

NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers. The N-terminal domain of subunit nqo5 (residues 1 to 153) wraps around nqo4 on one side, and the subunit interacts also with nqo9 via a two-stranded beta sheet (residues 154 to 171) and with nqo3 via an extended C-terminal loop (residues 172 to 196). Ref.3 Ref.4

Subcellular location

Cell membrane; Peripheral membrane protein; Cytoplasmic side HAMAP-Rule MF_01357.

Sequence similarities

Belongs to the complex I 30 kDa subunit family.

Ontologies

Keywords
   Biological processTransport
   Cellular componentCell membrane
Membrane
   LigandNAD
   Molecular functionOxidoreductase
   PTMQuinone
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processtransport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionNADH dehydrogenase (ubiquinone) activity

Inferred from electronic annotation. Source: InterPro

quinone binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 207207NADH-quinone oxidoreductase subunit 5 HAMAP-Rule MF_01357
PRO_0000118669

Secondary structure

........................................... 207
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q56219 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: EB6891BEF6F314AD

FASTA20723,859
        10         20         30         40         50         60 
MRLERVLEEA RAKGYPIEDN GLGNLWVVLP RERFKEEMAH YKAMGFNFLA DIVGLDYLTY 

        70         80         90        100        110        120 
PDPRPERFAV VYELVSLPGW KDGDGSRFFV RVYVPEEDPR LPTVTDLWGS ANFLEREVYD 

       130        140        150        160        170        180 
LFGIVFEGHP DLRKILTPED LEGHPLRKDY PLGETPTLFR EGRYIIPAEF RAALTGKDPG 

       190        200 
LTFYKGGSRK GYRSLWADLK KAREVKG 

« Hide

References

« Hide 'large scale' references
[1]"The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit."
Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T.
J. Biol. Chem. 272:4201-4211(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.
[3]"Identification of a novel subunit of respiratory complex I from Thermus thermophilus."
Hinchliffe P., Carroll J., Sazanov L.A.
Biochemistry 45:4413-4420(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-8, IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT.
[4]"Structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus."
Sazanov L.A., Hinchliffe P.
Science 311:1430-1436(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS), SUBUNIT, ELECTRON TRANSFER MECHANISM.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U52917 Genomic DNA. Translation: AAA97940.1.
AP008226 Genomic DNA. Translation: BAD69909.1.
PIRT11900.
RefSeqYP_143352.1. NC_006461.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2FUGX-ray3.305/E/N/W1-207[»]
2YBBelectron microscopy19.0051-207[»]
3I9VX-ray3.105/E1-207[»]
3IAMX-ray3.105/E1-207[»]
3IASX-ray3.155/E/N/W1-207[»]
3M9SX-ray4.505/E1-207[»]
4HEAX-ray3.305/F1-207[»]
ProteinModelPortalQ56219.
SMRQ56219. Positions 1-196.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-59263N.
STRING300852.TTHA0086.

Protein family/group databases

TCDB3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD69909; BAD69909; BAD69909.
GeneID3168317.
KEGGttj:TTHA0086.
PATRIC23955103. VBITheThe93045_0084.

Phylogenomic databases

eggNOGCOG0852.
HOGENOMHOG000099654.
KOK00332.
OMAYEERECH.
OrthoDBEOG60PHFK.
PhylomeDBQ56219.

Enzyme and pathway databases

BioCycTTHE300852:GH8R-90-MONOMER.

Family and domain databases

HAMAPMF_01357. NDH1_NuoC.
InterProIPR010218. NADH_DH_suC.
IPR001268. NADH_UbQ_OxRdtase_30kDa_su.
IPR020396. NADH_UbQ_OxRdtase_CS.
[Graphical view]
PfamPF00329. Complex1_30kDa. 1 hit.
[Graphical view]
ProDomPD001581. NADH_UbQ_OxRdtase_30kDa_su. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEPS00542. COMPLEX1_30K. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ56219.

Entry information

Entry nameNQO5_THET8
AccessionPrimary (citable) accession number: Q56219
Secondary accession number(s): Q5SM57
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references