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Q56219

- NQO5_THET8

UniProt

Q56219 - NQO5_THET8

Protein

NADH-quinone oxidoreductase subunit 5

Gene

nqo5

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. The nqo5 subunit may be involved in the stabilization of the complex.

    Catalytic activityi

    NADH + quinone = NAD+ + quinol.UniRule annotation

    GO - Molecular functioni

    1. NADH dehydrogenase (ubiquinone) activity Source: InterPro
    2. quinone binding Source: UniProtKB-KW

    GO - Biological processi

    1. transport Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Transport

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciTTHE300852:GH8R-90-MONOMER.

    Protein family/group databases

    TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH-quinone oxidoreductase subunit 5 (EC:1.6.99.5UniRule annotation)
    Alternative name(s):
    NADH dehydrogenase I chain 5
    NDH-1 subunit 5
    Gene namesi
    Name:nqo5
    Ordered Locus Names:TTHA0086
    OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
    Taxonomic identifieri300852 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
    ProteomesiUP000000532: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 207207NADH-quinone oxidoreductase subunit 5PRO_0000118669Add
    BLAST

    Keywords - PTMi

    Quinone

    Interactioni

    Subunit structurei

    NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers. The N-terminal domain of subunit nqo5 (residues 1 to 153) wraps around nqo4 on one side, and the subunit interacts also with nqo9 via a two-stranded beta sheet (residues 154 to 171) and with nqo3 via an extended C-terminal loop (residues 172 to 196).2 Publications

    Protein-protein interaction databases

    DIPiDIP-59263N.
    STRINGi300852.TTHA0086.

    Structurei

    Secondary structure

    1
    207
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 129
    Beta strandi17 – 193
    Beta strandi21 – 233
    Beta strandi25 – 284
    Helixi31 – 4313
    Beta strandi49 – 568
    Beta strandi65 – 7410
    Beta strandi80 – 823
    Beta strandi88 – 947
    Beta strandi100 – 1023
    Turni105 – 1073
    Helixi111 – 12010
    Beta strandi125 – 1273
    Beta strandi135 – 1373
    Beta strandi141 – 1433
    Beta strandi145 – 1473
    Beta strandi160 – 1623
    Helixi167 – 1693
    Beta strandi176 – 1783
    Beta strandi181 – 1833
    Helixi187 – 1893

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2FUGX-ray3.305/E/N/W1-207[»]
    2YBBelectron microscopy19.0051-207[»]
    3I9VX-ray3.105/E1-207[»]
    3IAMX-ray3.105/E1-207[»]
    3IASX-ray3.155/E/N/W1-207[»]
    3M9SX-ray4.505/E1-207[»]
    4HEAX-ray3.305/F1-207[»]
    ProteinModelPortaliQ56219.
    SMRiQ56219. Positions 1-196.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ56219.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the complex I 30 kDa subunit family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0852.
    HOGENOMiHOG000099654.
    KOiK00332.
    OMAiYEERECH.
    OrthoDBiEOG60PHFK.
    PhylomeDBiQ56219.

    Family and domain databases

    HAMAPiMF_01357. NDH1_NuoC.
    InterProiIPR010218. NADH_DH_suC.
    IPR001268. NADH_UbQ_OxRdtase_30kDa_su.
    IPR020396. NADH_UbQ_OxRdtase_CS.
    [Graphical view]
    PfamiPF00329. Complex1_30kDa. 1 hit.
    [Graphical view]
    ProDomiPD001581. NADH_UbQ_OxRdtase_30kDa_su. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    PROSITEiPS00542. COMPLEX1_30K. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q56219-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRLERVLEEA RAKGYPIEDN GLGNLWVVLP RERFKEEMAH YKAMGFNFLA    50
    DIVGLDYLTY PDPRPERFAV VYELVSLPGW KDGDGSRFFV RVYVPEEDPR 100
    LPTVTDLWGS ANFLEREVYD LFGIVFEGHP DLRKILTPED LEGHPLRKDY 150
    PLGETPTLFR EGRYIIPAEF RAALTGKDPG LTFYKGGSRK GYRSLWADLK 200
    KAREVKG 207
    Length:207
    Mass (Da):23,859
    Last modified:November 1, 1997 - v1
    Checksum:iEB6891BEF6F314AD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U52917 Genomic DNA. Translation: AAA97940.1.
    AP008226 Genomic DNA. Translation: BAD69909.1.
    PIRiT11900.
    RefSeqiWP_011174270.1. NC_006461.1.
    YP_143352.1. NC_006461.1.

    Genome annotation databases

    EnsemblBacteriaiBAD69909; BAD69909; BAD69909.
    GeneIDi3168317.
    KEGGittj:TTHA0086.
    PATRICi23955103. VBITheThe93045_0084.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U52917 Genomic DNA. Translation: AAA97940.1 .
    AP008226 Genomic DNA. Translation: BAD69909.1 .
    PIRi T11900.
    RefSeqi WP_011174270.1. NC_006461.1.
    YP_143352.1. NC_006461.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2FUG X-ray 3.30 5/E/N/W 1-207 [» ]
    2YBB electron microscopy 19.00 5 1-207 [» ]
    3I9V X-ray 3.10 5/E 1-207 [» ]
    3IAM X-ray 3.10 5/E 1-207 [» ]
    3IAS X-ray 3.15 5/E/N/W 1-207 [» ]
    3M9S X-ray 4.50 5/E 1-207 [» ]
    4HEA X-ray 3.30 5/F 1-207 [» ]
    ProteinModelPortali Q56219.
    SMRi Q56219. Positions 1-196.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-59263N.
    STRINGi 300852.TTHA0086.

    Protein family/group databases

    TCDBi 3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD69909 ; BAD69909 ; BAD69909 .
    GeneIDi 3168317.
    KEGGi ttj:TTHA0086.
    PATRICi 23955103. VBITheThe93045_0084.

    Phylogenomic databases

    eggNOGi COG0852.
    HOGENOMi HOG000099654.
    KOi K00332.
    OMAi YEERECH.
    OrthoDBi EOG60PHFK.
    PhylomeDBi Q56219.

    Enzyme and pathway databases

    BioCyci TTHE300852:GH8R-90-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q56219.

    Family and domain databases

    HAMAPi MF_01357. NDH1_NuoC.
    InterProi IPR010218. NADH_DH_suC.
    IPR001268. NADH_UbQ_OxRdtase_30kDa_su.
    IPR020396. NADH_UbQ_OxRdtase_CS.
    [Graphical view ]
    Pfami PF00329. Complex1_30kDa. 1 hit.
    [Graphical view ]
    ProDomi PD001581. NADH_UbQ_OxRdtase_30kDa_su. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    PROSITEi PS00542. COMPLEX1_30K. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit."
      Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T.
      J. Biol. Chem. 272:4201-4211(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete genome sequence of Thermus thermophilus HB8."
      Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HB8 / ATCC 27634 / DSM 579.
    3. "Identification of a novel subunit of respiratory complex I from Thermus thermophilus."
      Hinchliffe P., Carroll J., Sazanov L.A.
      Biochemistry 45:4413-4420(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-8, IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT.
    4. "Structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus."
      Sazanov L.A., Hinchliffe P.
      Science 311:1430-1436(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS), SUBUNIT, ELECTRON TRANSFER MECHANISM.

    Entry informationi

    Entry nameiNQO5_THET8
    AccessioniPrimary (citable) accession number: Q56219
    Secondary accession number(s): Q5SM57
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3