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Protein

NADH-quinone oxidoreductase subunit 6

Gene

nqo6

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.2 Publications

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.

Cofactori

[4Fe-4S] cluster1 PublicationNote: Binds 1 [4Fe-4S] cluster per subunit. This [4Fe-4S] cluster is referred to as N2.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi45 – 451Iron-sulfur (4Fe-4S)1 Publication
Metal bindingi46 – 461Iron-sulfur (4Fe-4S)1 Publication
Metal bindingi111 – 1111Iron-sulfur (4Fe-4S)1 Publication
Metal bindingi140 – 1401Iron-sulfur (4Fe-4S)1 Publication

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-89-MONOMER.

Protein family/group databases

TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit 6 (EC:1.6.5.11)
Alternative name(s):
NADH dehydrogenase I subunit 6
NDH-1 subunit 6
Gene namesi
Name:nqo6
Ordered Locus Names:TTHA0085
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

  • Cell membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 181180NADH-quinone oxidoreductase subunit 6PRO_0000118763Add
BLAST

Keywords - PTMi

Quinone

Interactioni

Subunit structurei

NDH-1 is composed of 15 different subunits, Nqo1 to Nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits Nqo7, Nqo8 and Nqo10 to Nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits Nqo1 to Nqo6, Nqo9 and Nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers. This subunit interacts extensively with Nqo4.2 Publications

Protein-protein interaction databases

DIPiDIP-59264N.
STRINGi300852.TTHA0085.

Structurei

Secondary structure

1
181
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi19 – 3214Combined sources
Beta strandi39 – 424Combined sources
Helixi46 – 505Combined sources
Turni51 – 566Combined sources
Helixi72 – 743Combined sources
Beta strandi78 – 825Combined sources
Turni86 – 883Combined sources
Helixi89 – 979Combined sources
Beta strandi105 – 1084Combined sources
Helixi109 – 1135Combined sources
Beta strandi120 – 1234Combined sources
Helixi127 – 1293Combined sources
Beta strandi134 – 1374Combined sources
Beta strandi139 – 1413Combined sources
Helixi144 – 15815Combined sources
Beta strandi159 – 1613Combined sources
Beta strandi165 – 1673Combined sources
Beta strandi176 – 1783Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FUGX-ray3.306/F/O/X1-181[»]
2YBBelectron microscopy19.0061-181[»]
3I9VX-ray3.106/F1-181[»]
3IAMX-ray3.106/F1-181[»]
3IASX-ray3.156/F/O/X1-181[»]
3M9SX-ray4.506/F1-181[»]
4HEAX-ray3.306/G1-181[»]
ProteinModelPortaliQ56218.
SMRiQ56218. Positions 15-175.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ56218.

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 20 kDa subunit family.Curated

Phylogenomic databases

eggNOGiENOG4105ES3. Bacteria.
COG0377. LUCA.
HOGENOMiHOG000228249.
KOiK00331.
OMAiDIDRFGM.
PhylomeDBiQ56218.

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
HAMAPiMF_01356. NDH1_NuoB. 1 hit.
InterProiIPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR006138. NADH_UQ_OxRdtase_20Kd_su.
[Graphical view]
PfamiPF01058. Oxidored_q6. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01957. nuoB_fam. 1 hit.
PROSITEiPS01150. COMPLEX1_20K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q56218-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALKDLFERD VQELEREGIL FTTLEKLVAW GRSNSLWPAT FGLACCAIEM
60 70 80 90 100
MASTDARNDL ARFGSEVFRA SPRQADVMIV AGRLSKKMAP VMRRVWEQMP
110 120 130 140 150
DPKWVISMGA CASSGGMFNN YAIVQNVDSV VPVDVYVPGC PPRPEALIYA
160 170 180
VMQLQKKVRG QAYNERGERL PPVAAWKRTR G
Length:181
Mass (Da):20,239
Last modified:January 23, 2007 - v3
Checksum:iA1E030366E6692F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52917 Genomic DNA. Translation: AAA97939.1.
AP008226 Genomic DNA. Translation: BAD69908.1.
PIRiT11899.
RefSeqiWP_011174271.1. NC_006461.1.
YP_143351.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD69908; BAD69908; BAD69908.
GeneIDi3168381.
KEGGittj:TTHA0085.
PATRICi23955101. VBITheThe93045_0083.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52917 Genomic DNA. Translation: AAA97939.1.
AP008226 Genomic DNA. Translation: BAD69908.1.
PIRiT11899.
RefSeqiWP_011174271.1. NC_006461.1.
YP_143351.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FUGX-ray3.306/F/O/X1-181[»]
2YBBelectron microscopy19.0061-181[»]
3I9VX-ray3.106/F1-181[»]
3IAMX-ray3.106/F1-181[»]
3IASX-ray3.156/F/O/X1-181[»]
3M9SX-ray4.506/F1-181[»]
4HEAX-ray3.306/G1-181[»]
ProteinModelPortaliQ56218.
SMRiQ56218. Positions 15-175.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59264N.
STRINGi300852.TTHA0085.

Protein family/group databases

TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD69908; BAD69908; BAD69908.
GeneIDi3168381.
KEGGittj:TTHA0085.
PATRICi23955101. VBITheThe93045_0083.

Phylogenomic databases

eggNOGiENOG4105ES3. Bacteria.
COG0377. LUCA.
HOGENOMiHOG000228249.
KOiK00331.
OMAiDIDRFGM.
PhylomeDBiQ56218.

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-89-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ56218.

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
HAMAPiMF_01356. NDH1_NuoB. 1 hit.
InterProiIPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR006138. NADH_UQ_OxRdtase_20Kd_su.
[Graphical view]
PfamiPF01058. Oxidored_q6. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01957. nuoB_fam. 1 hit.
PROSITEiPS01150. COMPLEX1_20K. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNQO6_THET8
AccessioniPrimary (citable) accession number: Q56218
Secondary accession number(s): Q5SM58
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.