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Q56218

- NQO6_THET8

UniProt

Q56218 - NQO6_THET8

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Protein

NADH-quinone oxidoreductase subunit 6

Gene

nqo6

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.

Catalytic activityi

NADH + quinone = NAD+ + quinol.

Cofactori

Binds 1 4Fe-4S cluster per subunit. This 4Fe-4S cluster is referred to as N2.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi45 – 451Iron-sulfur (4Fe-4S)
Metal bindingi46 – 461Iron-sulfur (4Fe-4S)
Metal bindingi111 – 1111Iron-sulfur (4Fe-4S)
Metal bindingi140 – 1401Iron-sulfur (4Fe-4S)

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. iron ion binding Source: UniProtKB-HAMAP
  3. NADH dehydrogenase (ubiquinone) activity Source: InterPro
  4. quinone binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-89-MONOMER.

Protein family/group databases

TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit 6 (EC:1.6.99.5)
Alternative name(s):
NADH dehydrogenase I subunit 6
NDH-1 subunit 6
Gene namesi
Name:nqo6
Ordered Locus Names:TTHA0085
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
ProteomesiUP000000532: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 181180NADH-quinone oxidoreductase subunit 6PRO_0000118763Add
BLAST

Keywords - PTMi

Quinone

Interactioni

Subunit structurei

NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers. Subunit nqo6 interacts extensively with nqo4.2 Publications

Protein-protein interaction databases

DIPiDIP-59264N.
STRINGi300852.TTHA0085.

Structurei

Secondary structure

1
181
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi19 – 3214
Beta strandi39 – 424
Helixi46 – 505
Turni51 – 566
Helixi72 – 743
Beta strandi78 – 825
Turni86 – 883
Helixi89 – 979
Beta strandi105 – 1084
Helixi109 – 1135
Beta strandi120 – 1234
Helixi127 – 1293
Beta strandi134 – 1374
Beta strandi139 – 1413
Helixi144 – 15815
Beta strandi159 – 1613
Beta strandi165 – 1673
Beta strandi176 – 1783

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FUGX-ray3.306/F/O/X1-181[»]
2YBBelectron microscopy19.0061-181[»]
3I9VX-ray3.106/F1-181[»]
3IAMX-ray3.106/F1-181[»]
3IASX-ray3.156/F/O/X1-181[»]
3M9SX-ray4.506/F1-181[»]
4HEAX-ray3.306/G1-181[»]
ProteinModelPortaliQ56218.
SMRiQ56218. Positions 15-175.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ56218.

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 20 kDa subunit family.Curated

Phylogenomic databases

eggNOGiCOG0377.
HOGENOMiHOG000228249.
KOiK00331.
OMAiDIDRFGM.
OrthoDBiEOG62K20C.
PhylomeDBiQ56218.

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
HAMAPiMF_01356. NDH1_NuoB.
InterProiIPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR006138. NADH_UQ_OxRdtase_20Kd_su.
[Graphical view]
PfamiPF01058. Oxidored_q6. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01957. nuoB_fam. 1 hit.
PROSITEiPS01150. COMPLEX1_20K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q56218 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALKDLFERD VQELEREGIL FTTLEKLVAW GRSNSLWPAT FGLACCAIEM
60 70 80 90 100
MASTDARNDL ARFGSEVFRA SPRQADVMIV AGRLSKKMAP VMRRVWEQMP
110 120 130 140 150
DPKWVISMGA CASSGGMFNN YAIVQNVDSV VPVDVYVPGC PPRPEALIYA
160 170 180
VMQLQKKVRG QAYNERGERL PPVAAWKRTR G
Length:181
Mass (Da):20,239
Last modified:January 23, 2007 - v3
Checksum:iA1E030366E6692F1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U52917 Genomic DNA. Translation: AAA97939.1.
AP008226 Genomic DNA. Translation: BAD69908.1.
PIRiT11899.
RefSeqiWP_011174271.1. NC_006461.1.
YP_143351.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD69908; BAD69908; BAD69908.
GeneIDi3168381.
KEGGittj:TTHA0085.
PATRICi23955101. VBITheThe93045_0083.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U52917 Genomic DNA. Translation: AAA97939.1 .
AP008226 Genomic DNA. Translation: BAD69908.1 .
PIRi T11899.
RefSeqi WP_011174271.1. NC_006461.1.
YP_143351.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2FUG X-ray 3.30 6/F/O/X 1-181 [» ]
2YBB electron microscopy 19.00 6 1-181 [» ]
3I9V X-ray 3.10 6/F 1-181 [» ]
3IAM X-ray 3.10 6/F 1-181 [» ]
3IAS X-ray 3.15 6/F/O/X 1-181 [» ]
3M9S X-ray 4.50 6/F 1-181 [» ]
4HEA X-ray 3.30 6/G 1-181 [» ]
ProteinModelPortali Q56218.
SMRi Q56218. Positions 15-175.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-59264N.
STRINGi 300852.TTHA0085.

Protein family/group databases

TCDBi 3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD69908 ; BAD69908 ; BAD69908 .
GeneIDi 3168381.
KEGGi ttj:TTHA0085.
PATRICi 23955101. VBITheThe93045_0083.

Phylogenomic databases

eggNOGi COG0377.
HOGENOMi HOG000228249.
KOi K00331.
OMAi DIDRFGM.
OrthoDBi EOG62K20C.
PhylomeDBi Q56218.

Enzyme and pathway databases

BioCyci TTHE300852:GH8R-89-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q56218.

Family and domain databases

Gene3Di 3.40.50.700. 1 hit.
HAMAPi MF_01356. NDH1_NuoB.
InterProi IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR006138. NADH_UQ_OxRdtase_20Kd_su.
[Graphical view ]
Pfami PF01058. Oxidored_q6. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01957. nuoB_fam. 1 hit.
PROSITEi PS01150. COMPLEX1_20K. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit."
    Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T.
    J. Biol. Chem. 272:4201-4211(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.
  3. "Identification of a novel subunit of respiratory complex I from Thermus thermophilus."
    Hinchliffe P., Carroll J., Sazanov L.A.
    Biochemistry 45:4413-4420(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-9, IDENTIFICATION BY MASS SPECTROMETRY, EPR SPECTROSCOPY, SUBUNIT.
  4. "Structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus."
    Sazanov L.A., Hinchliffe P.
    Science 311:1430-1436(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS), SUBUNIT, ELECTRON TRANSFER MECHANISM.

Entry informationi

Entry nameiNQO6_THET8
AccessioniPrimary (citable) accession number: Q56218
Secondary accession number(s): Q5SM58
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 105 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3