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Q56218

- NQO6_THET8

UniProt

Q56218 - NQO6_THET8

Protein

NADH-quinone oxidoreductase subunit 6

Gene

nqo6

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.

    Catalytic activityi

    NADH + quinone = NAD+ + quinol.

    Cofactori

    Binds 1 4Fe-4S cluster per subunit. This 4Fe-4S cluster is referred to as N2.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi45 – 451Iron-sulfur (4Fe-4S)
    Metal bindingi46 – 461Iron-sulfur (4Fe-4S)
    Metal bindingi111 – 1111Iron-sulfur (4Fe-4S)
    Metal bindingi140 – 1401Iron-sulfur (4Fe-4S)

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. iron ion binding Source: UniProtKB-HAMAP
    3. NADH dehydrogenase (ubiquinone) activity Source: InterPro
    4. quinone binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD

    Enzyme and pathway databases

    BioCyciTTHE300852:GH8R-89-MONOMER.

    Protein family/group databases

    TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH-quinone oxidoreductase subunit 6 (EC:1.6.99.5)
    Alternative name(s):
    NADH dehydrogenase I subunit 6
    NDH-1 subunit 6
    Gene namesi
    Name:nqo6
    Ordered Locus Names:TTHA0085
    OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
    Taxonomic identifieri300852 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
    ProteomesiUP000000532: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 181180NADH-quinone oxidoreductase subunit 6PRO_0000118763Add
    BLAST

    Keywords - PTMi

    Quinone

    Interactioni

    Subunit structurei

    NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers. Subunit nqo6 interacts extensively with nqo4.2 Publications

    Protein-protein interaction databases

    DIPiDIP-59264N.
    STRINGi300852.TTHA0085.

    Structurei

    Secondary structure

    1
    181
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi19 – 3214
    Beta strandi39 – 424
    Helixi46 – 505
    Turni51 – 566
    Helixi72 – 743
    Beta strandi78 – 825
    Turni86 – 883
    Helixi89 – 979
    Beta strandi105 – 1084
    Helixi109 – 1135
    Beta strandi120 – 1234
    Helixi127 – 1293
    Beta strandi134 – 1374
    Beta strandi139 – 1413
    Helixi144 – 15815
    Beta strandi159 – 1613
    Beta strandi165 – 1673
    Beta strandi176 – 1783

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2FUGX-ray3.306/F/O/X1-181[»]
    2YBBelectron microscopy19.0061-181[»]
    3I9VX-ray3.106/F1-181[»]
    3IAMX-ray3.106/F1-181[»]
    3IASX-ray3.156/F/O/X1-181[»]
    3M9SX-ray4.506/F1-181[»]
    4HEAX-ray3.306/G1-181[»]
    ProteinModelPortaliQ56218.
    SMRiQ56218. Positions 15-175.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ56218.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the complex I 20 kDa subunit family.Curated

    Phylogenomic databases

    eggNOGiCOG0377.
    HOGENOMiHOG000228249.
    KOiK00331.
    OMAiDIDRFGM.
    OrthoDBiEOG62K20C.
    PhylomeDBiQ56218.

    Family and domain databases

    Gene3Di3.40.50.700. 1 hit.
    HAMAPiMF_01356. NDH1_NuoB.
    InterProiIPR006137. NADH_UbQ_OxRdtase-like_20kDa.
    IPR006138. NADH_UQ_OxRdtase_20Kd_su.
    [Graphical view]
    PfamiPF01058. Oxidored_q6. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01957. nuoB_fam. 1 hit.
    PROSITEiPS01150. COMPLEX1_20K. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q56218-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALKDLFERD VQELEREGIL FTTLEKLVAW GRSNSLWPAT FGLACCAIEM    50
    MASTDARNDL ARFGSEVFRA SPRQADVMIV AGRLSKKMAP VMRRVWEQMP 100
    DPKWVISMGA CASSGGMFNN YAIVQNVDSV VPVDVYVPGC PPRPEALIYA 150
    VMQLQKKVRG QAYNERGERL PPVAAWKRTR G 181
    Length:181
    Mass (Da):20,239
    Last modified:January 23, 2007 - v3
    Checksum:iA1E030366E6692F1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U52917 Genomic DNA. Translation: AAA97939.1.
    AP008226 Genomic DNA. Translation: BAD69908.1.
    PIRiT11899.
    RefSeqiWP_011174271.1. NC_006461.1.
    YP_143351.1. NC_006461.1.

    Genome annotation databases

    EnsemblBacteriaiBAD69908; BAD69908; BAD69908.
    GeneIDi3168381.
    KEGGittj:TTHA0085.
    PATRICi23955101. VBITheThe93045_0083.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U52917 Genomic DNA. Translation: AAA97939.1 .
    AP008226 Genomic DNA. Translation: BAD69908.1 .
    PIRi T11899.
    RefSeqi WP_011174271.1. NC_006461.1.
    YP_143351.1. NC_006461.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2FUG X-ray 3.30 6/F/O/X 1-181 [» ]
    2YBB electron microscopy 19.00 6 1-181 [» ]
    3I9V X-ray 3.10 6/F 1-181 [» ]
    3IAM X-ray 3.10 6/F 1-181 [» ]
    3IAS X-ray 3.15 6/F/O/X 1-181 [» ]
    3M9S X-ray 4.50 6/F 1-181 [» ]
    4HEA X-ray 3.30 6/G 1-181 [» ]
    ProteinModelPortali Q56218.
    SMRi Q56218. Positions 15-175.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-59264N.
    STRINGi 300852.TTHA0085.

    Protein family/group databases

    TCDBi 3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD69908 ; BAD69908 ; BAD69908 .
    GeneIDi 3168381.
    KEGGi ttj:TTHA0085.
    PATRICi 23955101. VBITheThe93045_0083.

    Phylogenomic databases

    eggNOGi COG0377.
    HOGENOMi HOG000228249.
    KOi K00331.
    OMAi DIDRFGM.
    OrthoDBi EOG62K20C.
    PhylomeDBi Q56218.

    Enzyme and pathway databases

    BioCyci TTHE300852:GH8R-89-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q56218.

    Family and domain databases

    Gene3Di 3.40.50.700. 1 hit.
    HAMAPi MF_01356. NDH1_NuoB.
    InterProi IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
    IPR006138. NADH_UQ_OxRdtase_20Kd_su.
    [Graphical view ]
    Pfami PF01058. Oxidored_q6. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01957. nuoB_fam. 1 hit.
    PROSITEi PS01150. COMPLEX1_20K. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit."
      Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T.
      J. Biol. Chem. 272:4201-4211(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete genome sequence of Thermus thermophilus HB8."
      Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HB8 / ATCC 27634 / DSM 579.
    3. "Identification of a novel subunit of respiratory complex I from Thermus thermophilus."
      Hinchliffe P., Carroll J., Sazanov L.A.
      Biochemistry 45:4413-4420(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-9, IDENTIFICATION BY MASS SPECTROMETRY, EPR SPECTROSCOPY, SUBUNIT.
    4. "Structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus."
      Sazanov L.A., Hinchliffe P.
      Science 311:1430-1436(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS), SUBUNIT, ELECTRON TRANSFER MECHANISM.

    Entry informationi

    Entry nameiNQO6_THET8
    AccessioniPrimary (citable) accession number: Q56218
    Secondary accession number(s): Q5SM58
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 104 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3