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Protein

NADH-quinone oxidoreductase subunit 7

Gene

nqo7

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Transport

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-88-MONOMER.

Protein family/group databases

TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit 7 (EC:1.6.5.11)
Alternative name(s):
NADH dehydrogenase I chain 7
NDH-1 subunit 7
Gene namesi
Name:nqo7
Ordered Locus Names:TTHA0084
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
ProteomesiUP000000532 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei11 – 3121HelicalSequence AnalysisAdd
BLAST
Transmembranei59 – 7921HelicalSequence AnalysisAdd
BLAST
Transmembranei88 – 10821HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 119119NADH-quinone oxidoreductase subunit 7PRO_0000117861Add
BLAST

Keywords - PTMi

Quinone

Interactioni

Subunit structurei

NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.

Protein-protein interaction databases

DIPiDIP-59265N.
STRINGi300852.TTHA0084.

Structurei

Secondary structure

1
119
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 2824Combined sources
Helixi38 – 414Combined sources
Beta strandi44 – 474Combined sources
Helixi57 – 8327Combined sources
Turni86 – 883Combined sources
Helixi89 – 11325Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HE8X-ray3.30A/B1-119[»]
4HEAX-ray3.30A/P1-119[»]
ProteinModelPortaliQ56217.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I subunit 3 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0838.
HOGENOMiHOG000100119.
KOiK00330.
OMAiMKGALEW.
OrthoDBiEOG6QVRQ6.
PhylomeDBiQ56217.

Family and domain databases

HAMAPiMF_01394. NDH1_NuoA.
InterProiIPR023043. NAD(P)H_OxRDtase_bac/plastid.
IPR000440. NADH_UbQ/plastoQ_OxRdtase_su3.
[Graphical view]
PANTHERiPTHR11058. PTHR11058. 1 hit.
PfamiPF00507. Oxidored_q4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q56217-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPIQEYVGT LIYVGVALFI GVAALLVGAL LGPKKPGRAK LMPYESGNDP
60 70 80 90 100
AGEVKRFPVH FYVVAMLFIL FDVEVAFLWP YAVSAGGLGL YGFLGVLAFT
110
LLLFVGFLYE WWKGVMRWH
Length:119
Mass (Da):13,145
Last modified:November 1, 1997 - v1
Checksum:i10FFA8C204B0AFF5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52917 Genomic DNA. Translation: AAA97938.1.
AP008226 Genomic DNA. Translation: BAD69907.1.
PIRiT11898.
RefSeqiWP_011174272.1. NC_006461.1.
YP_143350.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD69907; BAD69907; BAD69907.
GeneIDi3168352.
KEGGittj:TTHA0084.
PATRICi23955099. VBITheThe93045_0082.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52917 Genomic DNA. Translation: AAA97938.1.
AP008226 Genomic DNA. Translation: BAD69907.1.
PIRiT11898.
RefSeqiWP_011174272.1. NC_006461.1.
YP_143350.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HE8X-ray3.30A/B1-119[»]
4HEAX-ray3.30A/P1-119[»]
ProteinModelPortaliQ56217.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59265N.
STRINGi300852.TTHA0084.

Protein family/group databases

TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD69907; BAD69907; BAD69907.
GeneIDi3168352.
KEGGittj:TTHA0084.
PATRICi23955099. VBITheThe93045_0082.

Phylogenomic databases

eggNOGiCOG0838.
HOGENOMiHOG000100119.
KOiK00330.
OMAiMKGALEW.
OrthoDBiEOG6QVRQ6.
PhylomeDBiQ56217.

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-88-MONOMER.

Family and domain databases

HAMAPiMF_01394. NDH1_NuoA.
InterProiIPR023043. NAD(P)H_OxRDtase_bac/plastid.
IPR000440. NADH_UbQ/plastoQ_OxRdtase_su3.
[Graphical view]
PANTHERiPTHR11058. PTHR11058. 1 hit.
PfamiPF00507. Oxidored_q4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit."
    Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T.
    J. Biol. Chem. 272:4201-4211(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.

Entry informationi

Entry nameiNQO7_THET8
AccessioniPrimary (citable) accession number: Q56217
Secondary accession number(s): Q5SM59
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 22, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.