Reviewed, UniProtKB/Swiss-Prot Q56217 (NQO7_THET8)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit 7
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I chain 7
    NDH-1 subunit 7

Gene names

Name:	nqo7
Ordered Locus Names:	TTHA0084

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Complete proteome] [HAMAP]

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	119 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. HAMAP MF_01394
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01394
Subunit structure	NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers. HAMAP MF_01394
Subcellular location	Cell inner membrane; Multi-pass membrane protein HAMAP MF_01394.
Sequence similarities	Belongs to the complex I subunit 3 family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: HAMAP photosynthesis, light reaction Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 119	119	NADH-quinone oxidoreductase subunit 7 HAMAP MF_01394		PRO_0000117861
Regions
	Transmembrane	11 – 31	21	Potential
	Transmembrane	59 – 79	21	Potential
	Transmembrane	88 – 108	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q56217-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 10FFA8C204B0AFF5	FASTA	119	13,145
	10 20 30 40 50 60 MAPIQEYVGT LIYVGVALFI GVAALLVGAL LGPKKPGRAK LMPYESGNDP AGEVKRFPVH 70 80 90 100 110 FYVVAMLFIL FDVEVAFLWP YAVSAGGLGL YGFLGVLAFT LLLFVGFLYE WWKGVMRWH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit." Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T. J. Biol. Chem. 272:4201-4211(1997) [PubMed: 9020134] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U52917 Genomic DNA. Translation: AAA97938.1. AP008226 Genomic DNA. Translation: BAD69907.1.
PIR	T11898.
RefSeq	YP_143350.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q56217.
Protein family/group databases
TCDB	3.D.1.3.1. H+ or Na+-translocating NADH dehydrogenase (NDH) family.
Genome annotation databases
GeneID	3168352.
GenomeReviews	Gene locus TTHA0084 in contig AP008226_GR.
KEGG	ttj:TTHA0084.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0838.
HOGENOM	HBG605540.
OMA	CEPVGSA.
Enzyme and pathway databases
BioCyc	TTHE300852:TTHA0084-MONOMER.
Family and domain databases
HAMAP	MF_01394. NDH1_NuoA. [Tree]
InterPro	IPR000440. NADH_UbQ/plastoQ_OxRdtase_su3. [Graphical view]
PANTHER	PTHR11058. Oxidored_q4. 1 hit.
Pfam	PF00507. Oxidored_q4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NQO7_THET8

Accession

Primary (citable) accession number: Q56217
Secondary accession number(s): Q5SM59

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 19, 2010
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)