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Q56214

- RUVB_THETH

UniProt

Q56214 - RUVB_THETH

Protein

Holliday junction ATP-dependent DNA helicase RuvB

Gene

ruvB

Organism
Thermus thermophilus
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvB is a Mg2+-dependent, DNA-dependent ATPase with an equal preference for supercoiled and linear duplex DNA. It can promote Holliday junction migration alone.

    Catalytic activityi

    ATP + H2O = ADP + phosphate.UniRule annotation

    Enzyme regulationi

    The activity of RuvB is enhanced by RuvA.

    Temperature dependencei

    Optimum temperature is 70 degrees Celsius.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi45 – 528ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. DNA binding Source: InterPro
    3. four-way junction helicase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA recombination Source: UniProtKB-HAMAP
    2. DNA repair Source: UniProtKB-HAMAP
    3. SOS response Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair, SOS response

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Holliday junction ATP-dependent DNA helicase RuvBUniRule annotation (EC:3.6.4.12UniRule annotation)
    Gene namesi
    Name:ruvBUniRule annotation
    OrganismiThermus thermophilus
    Taxonomic identifieri274 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 324324Holliday junction ATP-dependent DNA helicase RuvBPRO_0000165620Add
    BLAST

    Proteomic databases

    PRIDEiQ56214.

    Interactioni

    Subunit structurei

    Forms a complex with RuvA.

    Protein-protein interaction databases

    DIPiDIP-41108N.
    MINTiMINT-88046.

    Structurei

    3D structure databases

    ProteinModelPortaliQ56214.
    SMRiQ56214. Positions 4-318.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RuvB family.UniRule annotation

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPiMF_00016. DNA_helic_RuvB.
    InterProiIPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR004605. DNA_helicase_Holl-junc_RuvB.
    IPR008823. DNA_helicase_Holl-junc_RuvB_C.
    IPR027417. P-loop_NTPase.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00004. AAA. 1 hit.
    PF05491. RuvB_C. 1 hit.
    [Graphical view]
    ProDomiPD005323. DNA_helicase_Holl-junc_RuvB_C. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00635. ruvB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q56214-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEDLALRPKT LDEYIGQERL KQKLRVYLEA AKARKEPLEH LLLFGPPGLG    50
    KTTLAHVIAH ELGVNLRVTS GPAIEKPGDL AAILANSLEE GDILFIDEIH 100
    RLSRQAEEHL YPAMEDFVMD IVIGQGPAAR TIRLELPRFA LIGATTRPGL 150
    ITAPLLSRFG IVEHLEYYTP EELAQGVMRD ARLLGVRITE EAALEIGRRS 200
    RGTMRVAKRL FRRVRDFAQV EGEEVITRER ALEALAALGL DELGLEKRDR 250
    EILEVLILRF GAGPVGLATL ATALSEDPGT LEEVHEPYLI RQGLLKRTPR 300
    GRVATELAYR HLGYPPPVGP LLEP 324
    Length:324
    Mass (Da):36,016
    Last modified:November 1, 1997 - v1
    Checksum:i1B0DB92F373185CE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U22817 Genomic DNA. Translation: AAB03726.2.
    PIRiT11851.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U22817 Genomic DNA. Translation: AAB03726.2 .
    PIRi T11851.

    3D structure databases

    ProteinModelPortali Q56214.
    SMRi Q56214. Positions 4-318.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-41108N.
    MINTi MINT-88046.

    Proteomic databases

    PRIDEi Q56214.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPi MF_00016. DNA_helic_RuvB.
    InterProi IPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR004605. DNA_helicase_Holl-junc_RuvB.
    IPR008823. DNA_helicase_Holl-junc_RuvB_C.
    IPR027417. P-loop_NTPase.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00004. AAA. 1 hit.
    PF05491. RuvB_C. 1 hit.
    [Graphical view ]
    ProDomi PD005323. DNA_helicase_Holl-junc_RuvB_C. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00635. ruvB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, sequencing, and expression of ruvB and characterization of RuvB proteins from two distantly related thermophilic eubacteria."
      Tong J., Wetmur J.G.
      J. Bacteriol. 178:2695-2700(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiRUVB_THETH
    AccessioniPrimary (citable) accession number: Q56214
    Secondary accession number(s): Q9RA64
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3