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Q56214 (RUVB_THETH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Holliday junction ATP-dependent DNA helicase RuvB

EC=3.6.4.12
Gene names
Name:ruvB
OrganismThermus thermophilus
Taxonomic identifier274 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length324 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvB is a Mg2+-dependent, DNA-dependent ATPase with an equal preference for supercoiled and linear duplex DNA. It can promote Holliday junction migration alone. HAMAP-Rule MF_00016

Catalytic activity

ATP + H2O = ADP + phosphate. HAMAP-Rule MF_00016

Enzyme regulation

The activity of RuvB is enhanced by RuvA. HAMAP-Rule MF_00016

Subunit structure

Forms a complex with RuvA.

Sequence similarities

Belongs to the RuvB family.

Biophysicochemical properties

Temperature dependence:

Optimum temperature is 70 degrees Celsius. Ref.1

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 324324Holliday junction ATP-dependent DNA helicase RuvB HAMAP-Rule MF_00016
PRO_0000165620

Regions

Nucleotide binding45 – 528ATP Potential

Sequences

Sequence LengthMass (Da)Tools
Q56214 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 1B0DB92F373185CE

FASTA32436,016
        10         20         30         40         50         60 
MEDLALRPKT LDEYIGQERL KQKLRVYLEA AKARKEPLEH LLLFGPPGLG KTTLAHVIAH 

        70         80         90        100        110        120 
ELGVNLRVTS GPAIEKPGDL AAILANSLEE GDILFIDEIH RLSRQAEEHL YPAMEDFVMD 

       130        140        150        160        170        180 
IVIGQGPAAR TIRLELPRFA LIGATTRPGL ITAPLLSRFG IVEHLEYYTP EELAQGVMRD 

       190        200        210        220        230        240 
ARLLGVRITE EAALEIGRRS RGTMRVAKRL FRRVRDFAQV EGEEVITRER ALEALAALGL 

       250        260        270        280        290        300 
DELGLEKRDR EILEVLILRF GAGPVGLATL ATALSEDPGT LEEVHEPYLI RQGLLKRTPR 

       310        320 
GRVATELAYR HLGYPPPVGP LLEP 

« Hide

References

[1]"Cloning, sequencing, and expression of ruvB and characterization of RuvB proteins from two distantly related thermophilic eubacteria."
Tong J., Wetmur J.G.
J. Bacteriol. 178:2695-2700(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], BIOPHYSICOCHEMICAL PROPERTIES.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U22817 Genomic DNA. Translation: AAB03726.2.
PIRT11851.

3D structure databases

ProteinModelPortalQ56214.
SMRQ56214. Positions 4-318.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-88046.

Proteomic databases

PRIDEQ56214.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.10.10. 1 hit.
HAMAPMF_00016. DNA_helic_RuvB.
InterProIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR004605. DNA_helicase_Holl-junc_RuvB.
IPR008823. DNA_helicase_Holl-junc_RuvB_C.
IPR027417. P-loop_NTPase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00004. AAA. 1 hit.
PF05491. RuvB_C. 1 hit.
[Graphical view]
ProDomPD005323. DNA_helicase_Holl-junc_RuvB_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00635. ruvB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRUVB_THETH
AccessionPrimary (citable) accession number: Q56214
Secondary accession number(s): Q9RA64
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: February 19, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families