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Q56214

- RUVB_THETH

UniProt

Q56214 - RUVB_THETH

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Protein

Holliday junction ATP-dependent DNA helicase RuvB

Gene
ruvB
Organism
Thermus thermophilus
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvB is a Mg2+-dependent, DNA-dependent ATPase with an equal preference for supercoiled and linear duplex DNA. It can promote Holliday junction migration alone.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Enzyme regulationi

The activity of RuvB is enhanced by RuvA.UniRule annotation

Temperature dependencei

Optimum temperature is 70 degrees Celsius.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi45 – 528ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: InterPro
  3. four-way junction helicase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA recombination Source: UniProtKB-HAMAP
  2. DNA repair Source: UniProtKB-HAMAP
  3. SOS response Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, SOS response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Holliday junction ATP-dependent DNA helicase RuvB (EC:3.6.4.12)
Gene namesi
Name:ruvB
OrganismiThermus thermophilus
Taxonomic identifieri274 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 324324Holliday junction ATP-dependent DNA helicase RuvBUniRule annotationPRO_0000165620Add
BLAST

Proteomic databases

PRIDEiQ56214.

Interactioni

Subunit structurei

Forms a complex with RuvA.

Protein-protein interaction databases

MINTiMINT-88046.

Structurei

3D structure databases

ProteinModelPortaliQ56214.
SMRiQ56214. Positions 4-318.

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvB family.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00016. DNA_helic_RuvB.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR004605. DNA_helicase_Holl-junc_RuvB.
IPR008823. DNA_helicase_Holl-junc_RuvB_C.
IPR027417. P-loop_NTPase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF05491. RuvB_C. 1 hit.
[Graphical view]
ProDomiPD005323. DNA_helicase_Holl-junc_RuvB_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00635. ruvB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q56214-1 [UniParc]FASTAAdd to Basket

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MEDLALRPKT LDEYIGQERL KQKLRVYLEA AKARKEPLEH LLLFGPPGLG    50
KTTLAHVIAH ELGVNLRVTS GPAIEKPGDL AAILANSLEE GDILFIDEIH 100
RLSRQAEEHL YPAMEDFVMD IVIGQGPAAR TIRLELPRFA LIGATTRPGL 150
ITAPLLSRFG IVEHLEYYTP EELAQGVMRD ARLLGVRITE EAALEIGRRS 200
RGTMRVAKRL FRRVRDFAQV EGEEVITRER ALEALAALGL DELGLEKRDR 250
EILEVLILRF GAGPVGLATL ATALSEDPGT LEEVHEPYLI RQGLLKRTPR 300
GRVATELAYR HLGYPPPVGP LLEP 324
Length:324
Mass (Da):36,016
Last modified:November 1, 1997 - v1
Checksum:i1B0DB92F373185CE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U22817 Genomic DNA. Translation: AAB03726.2.
PIRiT11851.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U22817 Genomic DNA. Translation: AAB03726.2 .
PIRi T11851.

3D structure databases

ProteinModelPortali Q56214.
SMRi Q56214. Positions 4-318.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-88046.

Proteomic databases

PRIDEi Q56214.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPi MF_00016. DNA_helic_RuvB.
InterProi IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR004605. DNA_helicase_Holl-junc_RuvB.
IPR008823. DNA_helicase_Holl-junc_RuvB_C.
IPR027417. P-loop_NTPase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00004. AAA. 1 hit.
PF05491. RuvB_C. 1 hit.
[Graphical view ]
ProDomi PD005323. DNA_helicase_Holl-junc_RuvB_C. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00635. ruvB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning, sequencing, and expression of ruvB and characterization of RuvB proteins from two distantly related thermophilic eubacteria."
    Tong J., Wetmur J.G.
    J. Bacteriol. 178:2695-2700(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiRUVB_THETH
AccessioniPrimary (citable) accession number: Q56214
Secondary accession number(s): Q9RA64
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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