Q56214 (RUVB_THETH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Holliday junction ATP-dependent DNA helicase RuvB
EC=3.6.4.12

Gene names

Name:

ruvB

Organism

Thermus thermophilus

Taxonomic identifier

274 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvB is a Mg2+-dependent, DNA-dependent ATPase with an equal preference for supercoiled and linear duplex DNA. It can promote Holliday junction migration alone. HAMAP-Rule MF_00016
Catalytic activity	ATP + H2O = ADP + phosphate. HAMAP-Rule MF_00016
Enzyme regulation	The activity of RuvB is enhanced by RuvA. HAMAP-Rule MF_00016
Subunit structure	Forms a complex with RuvA.
Sequence similarities	Belongs to the RuvB family.
Biophysicochemical properties	Temperature dependence: Optimum temperature is 70 degrees Celsius. Ref.1

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair SOS response
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Gene Ontology (GO)
Biological_process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: InterPro four-way junction helicase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	Holliday junction ATP-dependent DNA helicase RuvB HAMAP-Rule MF_00016		PRO_0000165620
Regions
	Nucleotide binding	45 – 52	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q56214 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 1B0DB92F373185CE	FASTA	324	36,016
	10 20 30 40 50 60 MEDLALRPKT LDEYIGQERL KQKLRVYLEA AKARKEPLEH LLLFGPPGLG KTTLAHVIAH 70 80 90 100 110 120 ELGVNLRVTS GPAIEKPGDL AAILANSLEE GDILFIDEIH RLSRQAEEHL YPAMEDFVMD 130 140 150 160 170 180 IVIGQGPAAR TIRLELPRFA LIGATTRPGL ITAPLLSRFG IVEHLEYYTP EELAQGVMRD 190 200 210 220 230 240 ARLLGVRITE EAALEIGRRS RGTMRVAKRL FRRVRDFAQV EGEEVITRER ALEALAALGL 250 260 270 280 290 300 DELGLEKRDR EILEVLILRF GAGPVGLATL ATALSEDPGT LEEVHEPYLI RQGLLKRTPR 310 320 GRVATELAYR HLGYPPPVGP LLEP

References

[1]

"Cloning, sequencing, and expression of ruvB and characterization of RuvB proteins from two distantly related thermophilic eubacteria." Tong J., Wetmur J.G. J. Bacteriol. 178:2695-2700(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], BIOPHYSICOCHEMICAL PROPERTIES.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U22817 Genomic DNA. Translation: AAB03726.2.
PIR	T11851.
3D structure databases
ProteinModelPortal	Q56214.
SMR	Q56214. Positions 4-318.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-88046.
Proteomic databases
PRIDE	Q56214.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
HAMAP	MF_00016. DNA_helic_RuvB.
InterPro	IPR003593. AAA+_ATPase. IPR003959. ATPase_AAA_core. IPR004605. DNA_helicase_Holl-junc_RuvB. IPR008823. DNA_helicase_Holl-junc_RuvB_C. IPR027417. P-loop_NTPase. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
PANTHER	PTHR13779:SF2. PTHR13779:SF2. 1 hit.
Pfam	PF00004. AAA. 1 hit. PF05491. RuvB_C. 1 hit. [Graphical view]
ProDom	PD005323. DNA_helicase_Holl-junc_RuvB_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00382. AAA. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
TIGRFAMs	TIGR00635. ruvB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RUVB_THETH

Accession

Primary (citable) accession number: Q56214
Secondary accession number(s): Q9RA64

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 29, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program