Q56185 (Q56185_STRWE) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Epoxidase EMBL BAA32491.1

Gene names

Name:

fom4 EMBL BAA32491.1

Organism

Streptomyces wedmorensis EMBL BAA32491.1

Taxonomic identifier

43759 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	Iron PDB 1ZZ7 PDB 1ZZ8 PDB 1ZZ9 Metal-binding PDB 2BNM PDB 2BNN PDB 2BNO PDB 1ZZ7 PDB 1ZZ8 PDB 1ZZ9 Zinc PDB 2BNM PDB 2BNN PDB 2BNO
Technical term	3D-structure PDB 2BNM PDB 2BNN PDB 2BNO PDB 1ZZ7 PDB 1ZZ8 PDB 1ZZ9 PDB 1ZZC PDB 1ZZB PDB 1ZZ6
Gene Ontology (GO)
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	138	1	Iron; via tele nitrogen PDB 1ZZ7 PDB 1ZZ8 PDB 1ZZ9
	Metal binding	138	1	Zinc; via tele nitrogen PDB 2BNM PDB 2BNN PDB 2BNO
	Metal binding	142	1	Iron PDB 1ZZ7 PDB 1ZZ8 PDB 1ZZ9
	Metal binding	142	1	Zinc PDB 2BNM PDB 2BNN PDB 2BNO
	Metal binding	180	1	Iron; via tele nitrogen PDB 1ZZ7 PDB 1ZZ8 PDB 1ZZ9
	Metal binding	180	1	Zinc; via tele nitrogen PDB 2BNM PDB 2BNN PDB 2BNO

Sequences

	Sequence		Length	Mass (Da)
	Q56185 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 550D70A1D0D2FC56	FASTA	198	21,337
	10 20 30 40 50 60 MSNTKTASTG FAELLKDRRE QVKMDHAALA SLLGETPETV AAWENGEGGE LTLTQLGRIA 70 80 90 100 110 120 HVLGTSIGAL TPPAGNDLDD GVIIQMPDER PILKGVRDNV DYYVYNCLVR TKRAPSLVPL 130 140 150 160 170 180 VVDVLTDNPD DAKFNSGHAG NEFLFVLEGE IHMKWGDKEN PKEALLPTGA SMFVEEHVPH 190 AFTAAKGTGS AKLIAVNF

References

[1]	"Sequence of a P-methyltransferase-encoding gene isolated from a bialaphos-producing Streptomyces hygroscopicus." Hidaka T., Hidaka M., Kuzuyama T., Seto H. Gene 158:149-150(1995) [PubMed: 7789803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Nucleotide sequence of fortimicin KL1 methyltransferase gene isolated from Micromonospora olivasterospora, and comparison of its deduced amino acid sequence with those of methyltransferases involved in the biosynthesis of bialaphos and fosfomycin." Kuzuyama T., Seki T., Dairi T., Hidaka T., Seto H. J. Antibiot. 48:1191-1193(1995) [PubMed: 7490235] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[3]	"Cloning and nucleotide sequence of fosfomycin biosynthetic genes of Streptomyces wedmorensis." Hidaka T., Goda M., Kuzuyama T., Takei N., Hidaka M., Seto H. Mol. Gen. Genet. 249:274-280(1995) [PubMed: 7500951] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[4]	"Characterization of the fomA and fomB gene products from Streptomyces wedmorensis, which confer fosfomycin resistance on Escherichia coli." Kobayashi S., Kuzuyama T., Seto H. Antimicrob. Agents Chemother. 44:647-650(2000) [PubMed: 10681332] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[5]	"Structure and reactivity of hydroxypropylphosphonic acid epoxidase in fosfomycin biosynthesis by a cation- and flavin-dependent mechanism." McLuskey K., Cameron S., Hammerschmidt F., Hunter W.N. Proc. Natl. Acad. Sci. U.S.A. 102:14221-14226(2005) [PubMed: 16186494] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) IN COMPLEX WITH ZINC.
[6]	"Structural insight into antibiotic fosfomycin biosynthesis by a mononuclear iron enzyme." Higgins L.J., Yan F., Liu P., Liu H.W., Drennan C.L. Nature 437:838-844(2005) [PubMed: 16015285] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) IN COMPLEX WITH IRON.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB016934 Genomic DNA. Translation: BAA32491.1.

PIR

S60215.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ZZ6	X-ray	2.00	A/B	1-198	[»]
1ZZ7	X-ray	2.10	A/B	1-198	[»]
1ZZ8	X-ray	2.30	A/B/C	1-198	[»]
1ZZ9	X-ray	2.40	A/B/C	1-198	[»]
1ZZB	X-ray	2.30	A/B	1-198	[»]
1ZZC	X-ray	1.80	A/B	1-198	[»]
2BNM	X-ray	1.70	A/B	1-198	[»]
2BNN	X-ray	2.50	A/B	1-198	[»]
2BNO	X-ray	1.90	A/B	1-198	[»]
3SCF	X-ray	2.85	A/B/C	1-198	[»]
3SCG	X-ray	3.00	A/B/C	1-198	[»]
3SCH	X-ray	2.10	A/B	1-198	[»]

ProteinModelPortal

Q56185.

SMR

Q56185. Positions 5-198.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR013096. Cupin_2.
IPR011051. Cupin_RmlC_type.
IPR001387. HTH_3.
IPR010982. Lambda_DNA-bd.
IPR014710. RmlC-like_jellyroll.
[Graphical view]

Gene3D

G3DSA:1.10.260.40. G3DSA:1.10.260.40. 1 hit.
G3DSA:2.60.120.10. RmlC-like_jellyroll. 1 hit.

Pfam

PF07883. Cupin_2. 1 hit.
PF01381. HTH_3. 1 hit.
[Graphical view]

SMART

SM00530. HTH_XRE. 1 hit.
[Graphical view]

SUPFAM

SSF47413. Lambda_like_DNA. 1 hit.
SSF51182. RmlC_like_cupin. 1 hit.

PROSITE

PS50943. HTH_CROC1. 1 hit.
[Graphical view]

ProtoNet

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Entry information

Entry name

Q56185_STRWE

Accession

Primary (citable) accession number: Q56185

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1996
Last sequence update:	November 1, 1996
Last modified:	September 21, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)