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Protein

(S)-2-hydroxypropylphosphonic acid epoxidase

Gene

hppE

Organism
Streptomyces wedmorensis
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Non-heme-dependent dioxygenase that catalyzes the oxidative epoxidation of (S)-2-hydroxypropylphosphonate into (1R,2S)-epoxypropylphosphonate, the final step in the biosynthesis of fosfomycin antibiotic.2 Publications

Catalytic activityi

(S)-2-hydroxypropylphosphonate + H2O2 = (1R,2S)-1,2-epoxypropylphosphonate + 2 H2O.1 Publication

Cofactori

Fe2+1 PublicationNote: Binds 1 Fe2+ ion per subunit.1 Publication

Pathwayi: fosfomycin biosynthesis

This protein is involved in the pathway fosfomycin biosynthesis, which is part of Antibiotic biosynthesis.2 Publications
View all proteins of this organism that are known to be involved in the pathway fosfomycin biosynthesis and in Antibiotic biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei23SubstrateCombined sources2 Publications1
Binding sitei97SubstrateCombined sources3 Publications1
Binding sitei105SubstrateCombined sources3 Publications1
Metal bindingi138Iron; via tele nitrogenCombined sources3 Publications1
Metal bindingi142IronCombined sources3 Publications1
Binding sitei142SubstrateCombined sources3 Publications1
Metal bindingi180Iron; via tele nitrogenCombined sources3 Publications1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi26 – 45H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

  • antibiotic biosynthetic process Source: UniProtKB-KW
  • oxidation-reduction process Source: UniProtKB
  • phosphinothricin biosynthetic process Source: UniProtKB
  • protein homotetramerization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Antibiotic biosynthesis

Keywords - Ligandi

DNA-binding, Iron, Metal-binding, NAD

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13661.
BRENDAi1.11.1.23. 6118.
1.14.19.7. 6118.
UniPathwayiUPA01071.

Names & Taxonomyi

Protein namesi
Recommended name:
(S)-2-hydroxypropylphosphonic acid epoxidase (EC:1.11.1.231 Publication)
Alternative name(s):
Fosfomycin biosynthesis protein 4
Hydroxypropylphosphonate epoxidase
Short name:
Sw-hppE
Gene namesi
Name:hppE
Synonyms:fom4
OrganismiStreptomyces wedmorensis
Taxonomic identifieri43759 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi23K → A: Abolishes (S)-2-hydroxypropylphosphonic acid epoxidase activity. 1 Publication1
Mutagenesisi105Y → F: Abolishes (S)-2-hydroxypropylphosphonic acid epoxidase activity. 1 Publication1
Mutagenesisi142E → A: Abolishes (S)-2-hydroxypropylphosphonic acid epoxidase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004220301 – 198(S)-2-hydroxypropylphosphonic acid epoxidaseAdd BLAST198

Interactioni

Subunit structurei

Homotetramer.3 Publications

Structurei

Secondary structure

1198
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 21Combined sources16
Helixi26 – 33Combined sources8
Helixi37 – 44Combined sources8
Turni45 – 47Combined sources3
Helixi53 – 62Combined sources10
Helixi68 – 70Combined sources3
Beta strandi79 – 81Combined sources3
Helixi87 – 89Combined sources3
Beta strandi95 – 97Combined sources3
Beta strandi103 – 107Combined sources5
Beta strandi118 – 124Combined sources7
Helixi129 – 131Combined sources3
Beta strandi139 – 149Combined sources11
Beta strandi151 – 156Combined sources6
Beta strandi162 – 166Combined sources5
Beta strandi171 – 174Combined sources4
Beta strandi180 – 185Combined sources6
Beta strandi191 – 198Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZZ6X-ray2.00A/B1-198[»]
1ZZ7X-ray2.10A/B1-198[»]
1ZZ8X-ray2.30A/B/C1-198[»]
1ZZ9X-ray2.40A/B/C1-198[»]
1ZZBX-ray2.30A/B1-198[»]
1ZZCX-ray1.80A/B1-198[»]
2BNMX-ray1.70A/B1-198[»]
2BNNX-ray2.50A/B1-198[»]
2BNOX-ray1.90A/B1-198[»]
3SCFX-ray2.85A/B/C1-198[»]
3SCGX-ray3.00A/B/C1-198[»]
3SCHX-ray2.10A/B1-198[»]
4J1WX-ray2.72A/B/C1-198[»]
4J1XX-ray2.80A/B/C2-198[»]
ProteinModelPortaliQ56185.
SMRiQ56185.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ56185.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 70HTH cro/C1-typePROSITE-ProRule annotationAdd BLAST56

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni135 – 138Substrate bindingCombined sources3 Publications4

Sequence similaritiesi

Contains 1 HTH cro/C1-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

KOiK12903.

Family and domain databases

Gene3Di1.10.260.40. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR001387. Cro/C1-type_HTH.
IPR013096. Cupin_2.
IPR010982. Lambda_DNA-bd_dom.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF07883. Cupin_2. 1 hit.
PF01381. HTH_3. 1 hit.
[Graphical view]
SMARTiSM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMiSSF47413. SSF47413. 1 hit.
SSF51182. SSF51182. 1 hit.
PROSITEiPS50943. HTH_CROC1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q56185-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNTKTASTG FAELLKDRRE QVKMDHAALA SLLGETPETV AAWENGEGGE
60 70 80 90 100
LTLTQLGRIA HVLGTSIGAL TPPAGNDLDD GVIIQMPDER PILKGVRDNV
110 120 130 140 150
DYYVYNCLVR TKRAPSLVPL VVDVLTDNPD DAKFNSGHAG NEFLFVLEGE
160 170 180 190
IHMKWGDKEN PKEALLPTGA SMFVEEHVPH AFTAAKGTGS AKLIAVNF
Length:198
Mass (Da):21,337
Last modified:November 1, 1996 - v1
Checksum:i550D70A1D0D2FC56
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016934 Genomic DNA. Translation: BAA32491.1.
PIRiS60215.
RefSeqiWP_033205181.1. NZ_JNWK01000026.1.

Genome annotation databases

KEGGiag:BAA32491.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016934 Genomic DNA. Translation: BAA32491.1.
PIRiS60215.
RefSeqiWP_033205181.1. NZ_JNWK01000026.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZZ6X-ray2.00A/B1-198[»]
1ZZ7X-ray2.10A/B1-198[»]
1ZZ8X-ray2.30A/B/C1-198[»]
1ZZ9X-ray2.40A/B/C1-198[»]
1ZZBX-ray2.30A/B1-198[»]
1ZZCX-ray1.80A/B1-198[»]
2BNMX-ray1.70A/B1-198[»]
2BNNX-ray2.50A/B1-198[»]
2BNOX-ray1.90A/B1-198[»]
3SCFX-ray2.85A/B/C1-198[»]
3SCGX-ray3.00A/B/C1-198[»]
3SCHX-ray2.10A/B1-198[»]
4J1WX-ray2.72A/B/C1-198[»]
4J1XX-ray2.80A/B/C2-198[»]
ProteinModelPortaliQ56185.
SMRiQ56185.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:BAA32491.

Phylogenomic databases

KOiK12903.

Enzyme and pathway databases

UniPathwayiUPA01071.
BioCyciMetaCyc:MONOMER-13661.
BRENDAi1.11.1.23. 6118.
1.14.19.7. 6118.

Miscellaneous databases

EvolutionaryTraceiQ56185.

Family and domain databases

Gene3Di1.10.260.40. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR001387. Cro/C1-type_HTH.
IPR013096. Cupin_2.
IPR010982. Lambda_DNA-bd_dom.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF07883. Cupin_2. 1 hit.
PF01381. HTH_3. 1 hit.
[Graphical view]
SMARTiSM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMiSSF47413. SSF47413. 1 hit.
SSF51182. SSF51182. 1 hit.
PROSITEiPS50943. HTH_CROC1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHPPE_STRWE
AccessioniPrimary (citable) accession number: Q56185
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2013
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Phosphinothricin tripeptide (PTT) herbicide and fosfomycin antibiotic biosynthesis pathways share early steps starting with phosphoenolpyruvate before the pathways diverge after formation of 2-hydroxyethylphosphonate (HEP). HppE is involved in fosfomycin biosynthesis after divergence of the 2 pathways.1 Publication

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.