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Q56148

- CPT_STRVP

UniProt

Q56148 - CPT_STRVP

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Protein

Chloramphenicol 3-O phosphotransferase

Gene

SVEN_4064

Organism
Streptomyces venezuelae (strain ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Inactivates chloramphenicol by catalyzing the transfer of the gamma-phosphate of ATP to the antibiotic's C-3' hydroxyl group.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei37 – 371

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 178ATP

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Antibiotic resistance

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSVEN953739-WGS:GSXO-4129-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chloramphenicol 3-O phosphotransferase (EC:2.7.1.-)
Short name:
CPT
Gene namesi
Ordered Locus Names:SVEN_4064
OrganismiStreptomyces venezuelae (strain ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745)
Taxonomic identifieri953739 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces
ProteomesiUP000006854: Chromosome

Pathology & Biotechi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 178178Chloramphenicol 3-O phosphotransferasePRO_0000079295Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

1
178
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 95
Helixi16 – 2611
Beta strandi27 – 293
Beta strandi31 – 355
Helixi36 – 427
Helixi45 – 484
Beta strandi53 – 564
Turni58 – 603
Beta strandi62 – 643
Helixi66 – 8419
Beta strandi88 – 936
Turni96 – 983
Helixi99 – 10911
Beta strandi114 – 1207
Helixi123 – 13210
Beta strandi134 – 1363
Helixi140 – 1445
Helixi147 – 1493
Beta strandi155 – 1595
Turni160 – 1623
Helixi165 – 1739

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GRQX-ray2.90A1-178[»]
1GRRX-ray2.90A1-178[»]
1QHNX-ray2.70A1-178[»]
1QHSX-ray2.80A1-178[»]
1QHXX-ray2.50A1-178[»]
1QHYX-ray2.60A1-178[»]
ProteinModelPortaliQ56148.
SMRiQ56148. Positions 1-178.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ56148.

Family & Domainsi

Sequence similaritiesi

To M.tuberculosis Rv2636.Curated

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR012853. CPT.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF07931. CPT. 1 hit.
[Graphical view]
PIRSFiPIRSF007531. CPT. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q56148-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTRMIILNG GSSAGKSGIV RCLQSVLPEP WLAFGVDSLI EAMPLKMQSA
60 70 80 90 100
EGGIEFDADG GVSIGPEFRA LEGAWAEGVV AMARAGARII IDDVFLGGAA
110 120 130 140 150
AQERWRSFVG DLDVLWVGVR CDGAVAEGRE TARGDRVAGM AAKQAYVVHE
160 170
GVEYDVEVDT THKESIECAW AIAAHVVP
Length:178
Mass (Da):18,816
Last modified:November 1, 1996 - v1
Checksum:iFC12D650A1C5D51A
GO

Sequence cautioni

The sequence CCA57350.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U09991 Genomic DNA. Translation: AAB36569.1.
FR845719 Genomic DNA. Translation: CCA57350.1. Different initiation.
RefSeqiYP_006879609.1. NC_018750.1.

Genome annotation databases

EnsemblBacteriaiCCA57350; CCA57350; SVEN_4064.
GeneIDi13819222.
KEGGisve:SVEN_4064.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U09991 Genomic DNA. Translation: AAB36569.1 .
FR845719 Genomic DNA. Translation: CCA57350.1 . Different initiation.
RefSeqi YP_006879609.1. NC_018750.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1GRQ X-ray 2.90 A 1-178 [» ]
1GRR X-ray 2.90 A 1-178 [» ]
1QHN X-ray 2.70 A 1-178 [» ]
1QHS X-ray 2.80 A 1-178 [» ]
1QHX X-ray 2.50 A 1-178 [» ]
1QHY X-ray 2.60 A 1-178 [» ]
ProteinModelPortali Q56148.
SMRi Q56148. Positions 1-178.
ModBasei Search...
MobiDBi Search...

Chemistry

DrugBanki DB00446. Chloramphenicol.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CCA57350 ; CCA57350 ; SVEN_4064 .
GeneIDi 13819222.
KEGGi sve:SVEN_4064.

Enzyme and pathway databases

BioCyci SVEN953739-WGS:GSXO-4129-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q56148.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR012853. CPT.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF07931. CPT. 1 hit.
[Graphical view ]
PIRSFi PIRSF007531. CPT. 1 hit.
SUPFAMi SSF52540. SSF52540. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Inactivation of chloramphenicol by O-phosphorylation. A novel resistance mechanism in Streptomyces venezuelae ISP5230, a chloramphenicol producer."
    Mosher R.H., Camp D.J., Yang K., Brown M.P., Shaw W.V., Vining L.C.
    J. Biol. Chem. 270:27000-27006(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745.
  2. "Genome-wide analysis of the role of GlnR in Streptomyces venezuelae provides new insights into global nitrogen regulation in actinomycetes."
    Pullan S.T., Chandra G., Bibb M.J., Merrick M.
    BMC Genomics 12:175-175(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745.
  3. "The crystal structures of chloramphenicol phosphotransferase reveal a novel inactivation mechanism."
    Izard T., Ellis J.
    EMBO J. 19:2690-2700(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
    Strain: ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745.

Entry informationi

Entry nameiCPT_STRVP
AccessioniPrimary (citable) accession number: Q56148
Secondary accession number(s): F2RH22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: November 1, 1996
Last modified: October 1, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3