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Q56148

- CPT_STRVP

UniProt

Q56148 - CPT_STRVP

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Protein
Chloramphenicol 3-O phosphotransferase
Gene
SVEN_4064
Organism
Streptomyces venezuelae (strain ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Inactivates chloramphenicol by catalyzing the transfer of the gamma-phosphate of ATP to the antibiotic's C-3' hydroxyl group.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei37 – 371

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 178ATP

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Antibiotic resistance

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSVEN953739-WGS:GSXO-4129-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chloramphenicol 3-O phosphotransferase (EC:2.7.1.-)
Short name:
CPT
Gene namesi
Ordered Locus Names:SVEN_4064
OrganismiStreptomyces venezuelae (strain ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745)
Taxonomic identifieri953739 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces
ProteomesiUP000006854: Chromosome

Pathology & Biotechi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 178178Chloramphenicol 3-O phosphotransferase
PRO_0000079295Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 95
Helixi16 – 2611
Beta strandi27 – 293
Beta strandi31 – 355
Helixi36 – 427
Helixi45 – 484
Beta strandi53 – 564
Turni58 – 603
Beta strandi62 – 643
Helixi66 – 8419
Beta strandi88 – 936
Turni96 – 983
Helixi99 – 10911
Beta strandi114 – 1207
Helixi123 – 13210
Beta strandi134 – 1363
Helixi140 – 1445
Helixi147 – 1493
Beta strandi155 – 1595
Turni160 – 1623
Helixi165 – 1739

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GRQX-ray2.90A1-178[»]
1GRRX-ray2.90A1-178[»]
1QHNX-ray2.70A1-178[»]
1QHSX-ray2.80A1-178[»]
1QHXX-ray2.50A1-178[»]
1QHYX-ray2.60A1-178[»]
ProteinModelPortaliQ56148.
SMRiQ56148. Positions 1-178.

Miscellaneous databases

EvolutionaryTraceiQ56148.

Family & Domainsi

Sequence similaritiesi

To M.tuberculosis Rv2636.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR012853. CPT.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF07931. CPT. 1 hit.
[Graphical view]
PIRSFiPIRSF007531. CPT. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q56148-1 [UniParc]FASTAAdd to Basket

« Hide

MTTRMIILNG GSSAGKSGIV RCLQSVLPEP WLAFGVDSLI EAMPLKMQSA    50
EGGIEFDADG GVSIGPEFRA LEGAWAEGVV AMARAGARII IDDVFLGGAA 100
AQERWRSFVG DLDVLWVGVR CDGAVAEGRE TARGDRVAGM AAKQAYVVHE 150
GVEYDVEVDT THKESIECAW AIAAHVVP 178
Length:178
Mass (Da):18,816
Last modified:November 1, 1996 - v1
Checksum:iFC12D650A1C5D51A
GO

Sequence cautioni

The sequence CCA57350.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U09991 Genomic DNA. Translation: AAB36569.1.
FR845719 Genomic DNA. Translation: CCA57350.1. Different initiation.
RefSeqiYP_006879609.1. NC_018750.1.

Genome annotation databases

EnsemblBacteriaiCCA57350; CCA57350; SVEN_4064.
GeneIDi13819222.
KEGGisve:SVEN_4064.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U09991 Genomic DNA. Translation: AAB36569.1 .
FR845719 Genomic DNA. Translation: CCA57350.1 . Different initiation.
RefSeqi YP_006879609.1. NC_018750.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1GRQ X-ray 2.90 A 1-178 [» ]
1GRR X-ray 2.90 A 1-178 [» ]
1QHN X-ray 2.70 A 1-178 [» ]
1QHS X-ray 2.80 A 1-178 [» ]
1QHX X-ray 2.50 A 1-178 [» ]
1QHY X-ray 2.60 A 1-178 [» ]
ProteinModelPortali Q56148.
SMRi Q56148. Positions 1-178.
ModBasei Search...
MobiDBi Search...

Chemistry

DrugBanki DB00446. Chloramphenicol.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CCA57350 ; CCA57350 ; SVEN_4064 .
GeneIDi 13819222.
KEGGi sve:SVEN_4064.

Enzyme and pathway databases

BioCyci SVEN953739-WGS:GSXO-4129-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q56148.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR012853. CPT.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF07931. CPT. 1 hit.
[Graphical view ]
PIRSFi PIRSF007531. CPT. 1 hit.
SUPFAMi SSF52540. SSF52540. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Inactivation of chloramphenicol by O-phosphorylation. A novel resistance mechanism in Streptomyces venezuelae ISP5230, a chloramphenicol producer."
    Mosher R.H., Camp D.J., Yang K., Brown M.P., Shaw W.V., Vining L.C.
    J. Biol. Chem. 270:27000-27006(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745.
  2. "Genome-wide analysis of the role of GlnR in Streptomyces venezuelae provides new insights into global nitrogen regulation in actinomycetes."
    Pullan S.T., Chandra G., Bibb M.J., Merrick M.
    BMC Genomics 12:175-175(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745.
  3. "The crystal structures of chloramphenicol phosphotransferase reveal a novel inactivation mechanism."
    Izard T., Ellis J.
    EMBO J. 19:2690-2700(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
    Strain: ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745.

Entry informationi

Entry nameiCPT_STRVP
AccessioniPrimary (citable) accession number: Q56148
Secondary accession number(s): F2RH22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3

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