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Protein

Chloramphenicol 3-O phosphotransferase

Gene

SVEN_4064

Organism
Streptomyces venezuelae (strain ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Inactivates chloramphenicol by catalyzing the transfer of the gamma-phosphate of ATP to the antibiotic's C-3' hydroxyl group.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei371

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 17ATP8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Antibiotic resistance

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chloramphenicol 3-O phosphotransferase (EC:2.7.1.-)
Short name:
CPT
Gene namesi
Ordered Locus Names:SVEN_4064
OrganismiStreptomyces venezuelae (strain ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745)
Taxonomic identifieri953739 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces
Proteomesi
  • UP000006854 Componenti: Chromosome

Pathology & Biotechi

Chemistry databases

DrugBankiDB00446. Chloramphenicol.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000792951 – 178Chloramphenicol 3-O phosphotransferaseAdd BLAST178

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

1178
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 9Combined sources5
Helixi16 – 26Combined sources11
Beta strandi27 – 29Combined sources3
Beta strandi31 – 35Combined sources5
Helixi36 – 42Combined sources7
Helixi45 – 48Combined sources4
Beta strandi53 – 56Combined sources4
Turni58 – 60Combined sources3
Beta strandi62 – 64Combined sources3
Helixi66 – 84Combined sources19
Beta strandi88 – 93Combined sources6
Turni96 – 98Combined sources3
Helixi99 – 109Combined sources11
Beta strandi114 – 120Combined sources7
Helixi123 – 132Combined sources10
Beta strandi134 – 136Combined sources3
Helixi140 – 144Combined sources5
Helixi147 – 149Combined sources3
Beta strandi155 – 159Combined sources5
Turni160 – 162Combined sources3
Helixi165 – 173Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GRQX-ray2.90A1-178[»]
1GRRX-ray2.90A1-178[»]
1QHNX-ray2.70A1-178[»]
1QHSX-ray2.80A1-178[»]
1QHXX-ray2.50A1-178[»]
1QHYX-ray2.60A1-178[»]
ProteinModelPortaliQ56148.
SMRiQ56148.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ56148.

Family & Domainsi

Sequence similaritiesi

To M.tuberculosis Rv2636.Curated

Phylogenomic databases

KOiK18554.
OrthoDBiPOG091H0L09.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR012853. CPT.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF07931. CPT. 1 hit.
[Graphical view]
PIRSFiPIRSF007531. CPT. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q56148-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTRMIILNG GSSAGKSGIV RCLQSVLPEP WLAFGVDSLI EAMPLKMQSA
60 70 80 90 100
EGGIEFDADG GVSIGPEFRA LEGAWAEGVV AMARAGARII IDDVFLGGAA
110 120 130 140 150
AQERWRSFVG DLDVLWVGVR CDGAVAEGRE TARGDRVAGM AAKQAYVVHE
160 170
GVEYDVEVDT THKESIECAW AIAAHVVP
Length:178
Mass (Da):18,816
Last modified:November 1, 1996 - v1
Checksum:iFC12D650A1C5D51A
GO

Sequence cautioni

The sequence CCA57350 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09991 Genomic DNA. Translation: AAB36569.1.
FR845719 Genomic DNA. Translation: CCA57350.1. Different initiation.
RefSeqiWP_063844287.1. NG_047672.1.

Genome annotation databases

EnsemblBacteriaiCCA57350; CCA57350; SVEN_4064.
KEGGiag:AAB36569.
sve:SVEN_4064.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09991 Genomic DNA. Translation: AAB36569.1.
FR845719 Genomic DNA. Translation: CCA57350.1. Different initiation.
RefSeqiWP_063844287.1. NG_047672.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GRQX-ray2.90A1-178[»]
1GRRX-ray2.90A1-178[»]
1QHNX-ray2.70A1-178[»]
1QHSX-ray2.80A1-178[»]
1QHXX-ray2.50A1-178[»]
1QHYX-ray2.60A1-178[»]
ProteinModelPortaliQ56148.
SMRiQ56148.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

DrugBankiDB00446. Chloramphenicol.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCA57350; CCA57350; SVEN_4064.
KEGGiag:AAB36569.
sve:SVEN_4064.

Phylogenomic databases

KOiK18554.
OrthoDBiPOG091H0L09.

Miscellaneous databases

EvolutionaryTraceiQ56148.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR012853. CPT.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF07931. CPT. 1 hit.
[Graphical view]
PIRSFiPIRSF007531. CPT. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCPT_STRVP
AccessioniPrimary (citable) accession number: Q56148
Secondary accession number(s): F2RH22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.