Q56148 (CPT_STRVP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chloramphenicol 3-O phosphotransferase
Short name=CPT
EC=2.7.1.-

Gene names

Ordered Locus Names:

SVEN_4064

Organism

Streptomyces venezuelae (strain ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745) [Complete proteome] [HAMAP]

Taxonomic identifier

953739 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces › Streptomyces venezuelae

Protein attributes

Sequence length	178 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inactivates chloramphenicol by catalyzing the transfer of the gamma-phosphate of ATP to the antibiotic's C-3' hydroxyl group.
Subunit structure	Homodimer.
Sequence similarities	To M.tuberculosis Rv2636.
Sequence caution	The sequence CCA57350.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Antibiotic resistance
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological_process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 178

178

Chloramphenicol 3-O phosphotransferase

PRO_0000079295

Regions

Nucleotide binding

10 – 17

8

ATP

Sites

Active site

37

1

Secondary structure

1

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178

Helix Strand Turn

Details...

Beta strand

5 – 9

5

Helix

16 – 26

11

Beta strand

27 – 29

3

Beta strand

31 – 35

5

Helix

36 – 42

7

Helix

45 – 48

4

Beta strand

53 – 56

4

Turn

58 – 60

3

Beta strand

62 – 64

3

Helix

66 – 84

19

Beta strand

88 – 93

6

Turn

96 – 98

3

Helix

99 – 109

11

Beta strand

114 – 120

7

Helix

123 – 132

10

Beta strand

134 – 136

3

Helix

140 – 144

5

Helix

147 – 149

3

Beta strand

155 – 159

5

Turn

160 – 162

3

Helix

165 – 173

9

Sequences

	Sequence		Length	Mass (Da)
	Q56148 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: FC12D650A1C5D51A	FASTA	178	18,816
	10 20 30 40 50 60 MTTRMIILNG GSSAGKSGIV RCLQSVLPEP WLAFGVDSLI EAMPLKMQSA EGGIEFDADG 70 80 90 100 110 120 GVSIGPEFRA LEGAWAEGVV AMARAGARII IDDVFLGGAA AQERWRSFVG DLDVLWVGVR 130 140 150 160 170 CDGAVAEGRE TARGDRVAGM AAKQAYVVHE GVEYDVEVDT THKESIECAW AIAAHVVP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Inactivation of chloramphenicol by O-phosphorylation. A novel resistance mechanism in Streptomyces venezuelae ISP5230, a chloramphenicol producer." Mosher R.H., Camp D.J., Yang K., Brown M.P., Shaw W.V., Vining L.C. J. Biol. Chem. 270:27000-27006(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745.
[2]	"Genome-wide analysis of the role of GlnR in Streptomyces venezuelae provides new insights into global nitrogen regulation in actinomycetes." Pullan S.T., Chandra G., Bibb M.J., Merrick M. BMC Genomics 12:175-175(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745.
[3]	"The crystal structures of chloramphenicol phosphotransferase reveal a novel inactivation mechanism." Izard T., Ellis J. EMBO J. 19:2690-2700(2000) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS). Strain: ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U09991 Genomic DNA. Translation: AAB36569.1.
FR845719 Genomic DNA. Translation: CCA57350.1. Different initiation.

RefSeq

YP_006879609.1. NC_018750.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GRQ	X-ray	2.90	A	1-178	[»]
1GRR	X-ray	2.90	A	1-178	[»]
1QHN	X-ray	2.70	A	1-178	[»]
1QHS	X-ray	2.80	A	1-178	[»]
1QHX	X-ray	2.50	A	1-178	[»]
1QHY	X-ray	2.60	A	1-178	[»]

ProteinModelPortal

Q56148.

SMR

Q56148. Positions 1-178.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

CCA57350; CCA57350; SVEN_4064.

GeneID

13819222.

KEGG

sve:SVEN_4064.

Organism-specific databases

CMR

Search...

Family and domain databases

InterPro

IPR012853. CPT.
IPR027417. P-loop_NTPase.
[Graphical view]

Pfam

PF07931. CPT. 1 hit.
[Graphical view]

PIRSF

PIRSF007531. CPT. 1 hit.

SUPFAM

SSF52540. SSF52540. 1 hit.

ProtoNet

Search...

Other

DrugBank

DB00446. Chloramphenicol.

EvolutionaryTrace

Q56148.

Entry information

Entry name

CPT_STRVP

Accession

Primary (citable) accession number: Q56148
Secondary accession number(s): F2RH22

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program