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Protein

Cell invasion protein SipB

Gene

sipB

Organism
Salmonella typhi
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Required for entry into the host cell through presentation or delivery of SipC at the host cell plasma membrane. Along with SipC, is necessary for the transfer of other effector proteins into the host cell. Induces macrophage apoptosis either by binding and activating the proapoptotic enzyme caspase-1 (caspase-1 dependent), resulting in the release of interleukin-1 beta active form, or by disrupting mitochondria and inducing autophagy (caspase-1 independent). The former is dependent of its membrane-fusion activity. The SipBC complex, in association with its chaperone SicA, is regulated by binding of InvE (By similarity).By similarity1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
Cell invasion protein SipB
Alternative name(s):
Effector protein SipB
Gene namesi
Name:sipB
Ordered Locus Names:STY3008, t2787
OrganismiSalmonella typhi
Taxonomic identifieri90370 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000002670 Componenti: Chromosome
  • UP000000541 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei320 – 35435HelicalBy similarityAdd
BLAST
Transmembranei408 – 42720HelicalBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 593593Cell invasion protein SipBPRO_0000219855Add
BLAST

Interactioni

Subunit structurei

Homotrimer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Casp1P294522EBI-489689,EBI-489700From a different organism.

Protein-protein interaction databases

IntActiQ56134. 1 interaction.
STRINGi220341.STY3008.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili151 – 20858Sequence analysisAdd
BLAST
Coiled coili287 – 31428Sequence analysisAdd
BLAST

Domaini

SipB membrane integration requires both hydrophobic domains and the helical C-terminal region.By similarity

Sequence similaritiesi

Belongs to the invasin protein B family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4106H1J. Bacteria.
ENOG410XWFQ. LUCA.
HOGENOMiHOG000028231.
KOiK13285.
OMAiRNATATF.
OrthoDBiEOG6WHNNM.

Family and domain databases

InterProiIPR032391. SipB_N.
IPR006972. SseC.
IPR003895. T3SS_invasion_prot_B.
[Graphical view]
PfamiPF04888. SseC. 1 hit.
PF16535. T3SSipB. 1 hit.
[Graphical view]
PRINTSiPR01375. BACINVASINB.

Sequencei

Sequence statusi: Complete.

Q56134-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNDASSISR SGYTQNPRLA EAAFEGVRKN TDFLKAADKA FKDVVATKAG
60 70 80 90 100
DLKAGTKSGE SAINTVGLKP PTDAAREKLS SEGQLTLLLG KLMTLLGDVS
110 120 130 140 150
LSQLESRLAV WQAMIESQKE MGIQVSKEFQ TALGEAQEAT DLYEASIKKT
160 170 180 190 200
DTAKSVYDAA AKKLTQAQNK LQSLDPADPG YAQAEAAVEQ AGKEATEAKE
210 220 230 240 250
ALDKATDATV KAGTDAKAKA EKADNILTKF QGTANAASQN QVSQGEQDNL
260 270 280 290 300
SNVARLTMLM AMFIEIVGKN TEESLQNDLA LFNALQEGRQ AEMEKKSAEF
310 320 330 340 350
QEETRKAEET NRIMGCIGKV LGALLTIVSV VAAVFTGGAS LALAAVGLAV
360 370 380 390 400
MVADEIVKAA TGVSFIQQAL NPIMEHVLKP LMELIGKAIT KALEGLGVDK
410 420 430 440 450
KTAEMAGSIV GAIVAAIAMV AVIVVVAVVG KGAAAKLGNA LSKMMGETIK
460 470 480 490 500
KLVPNVLKQL AQNGSKLFTQ GMQRITSGLG NVGSKMGLQT NALSKELVGN
510 520 530 540 550
TLNKVALGME VTNTAAQSAG GVAEGVFIKN ASEALADFML ARFAMDQIQQ
560 570 580 590
WLKQSVEIFG ENQKVTAELQ KAMSSAVQQN ADASRFILRQ SRA
Length:593
Mass (Da):62,421
Last modified:November 1, 1996 - v1
Checksum:i8570D64343276639
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82670 Genomic DNA. Translation: CAA57988.1.
AL513382 Genomic DNA. Translation: CAD05992.1.
AE014613 Genomic DNA. Translation: AAO70348.1.
PIRiS70216.
RefSeqiNP_457279.1. NC_003198.1.
WP_000245782.1. NZ_FFOG01000005.1.

Genome annotation databases

EnsemblBacteriaiAAO70348; AAO70348; t2787.
CAD05992; CAD05992; CAD05992.
GeneIDi1249314.
KEGGistt:t2787.
sty:STY3008.
PATRICi18544064. VBISalEnt120419_3062.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82670 Genomic DNA. Translation: CAA57988.1.
AL513382 Genomic DNA. Translation: CAD05992.1.
AE014613 Genomic DNA. Translation: AAO70348.1.
PIRiS70216.
RefSeqiNP_457279.1. NC_003198.1.
WP_000245782.1. NZ_FFOG01000005.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ56134. 1 interaction.
STRINGi220341.STY3008.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO70348; AAO70348; t2787.
CAD05992; CAD05992; CAD05992.
GeneIDi1249314.
KEGGistt:t2787.
sty:STY3008.
PATRICi18544064. VBISalEnt120419_3062.

Phylogenomic databases

eggNOGiENOG4106H1J. Bacteria.
ENOG410XWFQ. LUCA.
HOGENOMiHOG000028231.
KOiK13285.
OMAiRNATATF.
OrthoDBiEOG6WHNNM.

Family and domain databases

InterProiIPR032391. SipB_N.
IPR006972. SseC.
IPR003895. T3SS_invasion_prot_B.
[Graphical view]
PfamiPF04888. SseC. 1 hit.
PF16535. T3SSipB. 1 hit.
[Graphical view]
PRINTSiPR01375. BACINVASINB.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Functional conservation of the Salmonella and Shigella effectors of entry into epithelial cells."
    Hermant D., Menard R., Arricau N., Parsot C., Popoff M.Y.
    Mol. Microbiol. 17:781-789(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 700931 / Ty2.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CT18.
  3. "Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18."
    Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R.
    J. Bacteriol. 185:2330-2337(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700931 / Ty2.
  4. "The Salmonella invasin SipB induces macrophage apoptosis by binding to caspase-1."
    Hersh D., Monack D.M., Smith M.R., Ghori N., Falkow S., Zychlinsky A.
    Proc. Natl. Acad. Sci. U.S.A. 96:2396-2401(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN CASPASE-1-DEPENDENT APOPTOSIS.
    Strain: ATCC 700931 / Ty2.

Entry informationi

Entry nameiSIPB_SALTI
AccessioniPrimary (citable) accession number: Q56134
Secondary accession number(s): Q7AME0, Q7C7N1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.