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Protein

Secreted effector protein SifA

Gene

sifA

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein is required for endosomal tubulation and formation of Salmonella-induced filaments (Sifs), which are filamentous structures containing lysosomal membrane glycoproteins within epithelial cells. Sif formation is concomitant with intracellular bacterial replication.3 Publications

GO - Biological processi

  • pathogenesis Source: UniProtKB-KW
  • regulation of protein localization Source: AgBase
Complete GO annotation...

Keywords - Biological processi

Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
Secreted effector protein SifA
Gene namesi
Name:sifA
Ordered Locus Names:STM1224
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

  • Secreted
  • Host cytoplasm
  • Host cell membrane

  • Note: Secreted via type III secretion system 2 (SPI-2 TTSS), and delivered into the host cytoplasm. It associates with membranes and colocalizes with microtubules along the length of Sifs in infected cells.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host cytoplasm, Host membrane, Membrane, Secreted

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene do not produce Sifs in epithelial cells and show attenuated virulence in mice.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi130L → D: Loss of interaction with host PLEKHM2. Fails to induce host endosomal tubulation; when associated with D-131. 1 Publication1
Mutagenesisi131M → D: Alters interaction with host PLEKHM2. Fails to induce host endosomal tubulation; when associated with D-130. 1 Publication1
Mutagenesisi197W → A: Fails to induce host endosomal tubulation; when associated with A-201. 1 Publication1
Mutagenesisi201E → A: Fails to induce host endosomal tubulation; when associated with A-197. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000977601 – 336Secreted effector protein SifAAdd BLAST336

Proteomic databases

PaxDbiQ56061.

Interactioni

Subunit structurei

Interacts with host PLEKHM2. Interacts with SseJ; the interaction is indirect.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PLEKHM2Q8IWE53EBI-10765408,EBI-726484From a different organism.

Protein-protein interaction databases

DIPiDIP-46411N.
IntActiQ56061. 2 interactors.
STRINGi99287.STM1224.

Structurei

Secondary structure

1336
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi25 – 34Combined sources10
Helixi35 – 37Combined sources3
Helixi42 – 54Combined sources13
Beta strandi55 – 58Combined sources4
Helixi62 – 75Combined sources14
Helixi78 – 81Combined sources4
Beta strandi84 – 87Combined sources4
Helixi89 – 91Combined sources3
Beta strandi96 – 101Combined sources6
Beta strandi107 – 114Combined sources8
Beta strandi116 – 123Combined sources8
Beta strandi126 – 132Combined sources7
Beta strandi145 – 153Combined sources9
Turni154 – 157Combined sources4
Beta strandi158 – 167Combined sources10
Beta strandi169 – 171Combined sources3
Beta strandi174 – 177Combined sources4
Helixi180 – 187Combined sources8
Beta strandi190 – 192Combined sources3
Turni194 – 196Combined sources3
Helixi197 – 220Combined sources24
Turni221 – 223Combined sources3
Helixi228 – 241Combined sources14
Helixi258 – 267Combined sources10
Turni270 – 272Combined sources3
Helixi273 – 276Combined sources4
Helixi283 – 303Combined sources21
Helixi307 – 324Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CXBX-ray2.60A1-336[»]
3HW2X-ray3.30A1-336[»]
SMRiQ56061.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ56061.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 330Interaction with host PLEKHM2Add BLAST330

Domaini

Domains throughout the protein, and not only the N-terminus, are required for secretion and translocation. Both N- and C-terminal domains are also required for formation of tubules.1 Publication

Sequence similaritiesi

Belongs to the Sif family.Curated

Phylogenomic databases

HOGENOMiHOG000028279.
KOiK15347.
OMAiLETMTMC.

Family and domain databases

InterProiIPR010637. Sif.
[Graphical view]
PfamiPF06767. Sif. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q56061-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPITIGNGFL KSEILTNSPR NTKEAWWKVL WEKIKDFFFS TGKAKADRCL
60 70 80 90 100
HEMLFAERAP TRERLTEIFF ELKELACASQ RDRFQVHNPH ENDATIILRI
110 120 130 140 150
MDQNEENELL RITQNTDTFS CEVMGNLYFL MKDRPDILKS HPQMTAMIKR
160 170 180 190 200
RYSEIVDYPL PSTLCLNPAG APILSVPLDN IEGYLYTELR KGHLDGWKAQ
210 220 230 240 250
EKATYLAAKI QSGIEKTTRI LHHANISEST QQNAFLETMA MCGLKQLEIP
260 270 280 290 300
PPHTHIPIEK MVKEVLLADK TFQAFLVTDP STSQSMLAEI VEAISDQVFH
310 320 330
AIFRIDPQAI QKMAEEQLTT LHVRSEQQSG CLCCFL
Length:336
Mass (Da):38,498
Last modified:November 1, 1997 - v1
Checksum:i83C6185148A774B9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51867 Genomic DNA. Translation: AAA97467.1.
AE006468 Genomic DNA. Translation: AAL20153.1.
PIRiS71033.
RefSeqiNP_460194.1. NC_003197.1.
WP_001520468.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL20153; AAL20153; STM1224.
GeneIDi1252742.
KEGGistm:STM1224.
PATRICi32380899. VBISalEnt20916_1294.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51867 Genomic DNA. Translation: AAA97467.1.
AE006468 Genomic DNA. Translation: AAL20153.1.
PIRiS71033.
RefSeqiNP_460194.1. NC_003197.1.
WP_001520468.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CXBX-ray2.60A1-336[»]
3HW2X-ray3.30A1-336[»]
SMRiQ56061.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46411N.
IntActiQ56061. 2 interactors.
STRINGi99287.STM1224.

Proteomic databases

PaxDbiQ56061.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL20153; AAL20153; STM1224.
GeneIDi1252742.
KEGGistm:STM1224.
PATRICi32380899. VBISalEnt20916_1294.

Phylogenomic databases

HOGENOMiHOG000028279.
KOiK15347.
OMAiLETMTMC.

Miscellaneous databases

EvolutionaryTraceiQ56061.

Family and domain databases

InterProiIPR010637. Sif.
[Graphical view]
PfamiPF06767. Sif. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSIFA_SALTY
AccessioniPrimary (citable) accession number: Q56061
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.