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Q56056 (RNC_SALTY) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonuclease 3

EC=3.1.26.3
Alternative name(s):
Ribonuclease III
Short name=RNase III
Gene names
Name:rnc
Ordered Locus Names:STM2581
OrganismSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]
Taxonomic identifier99287 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Protein attributes

Sequence length226 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Digests double-stranded RNA. Involved in the processing of ribosomal RNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Removes small helical intervening sequences (IVSs) from all 7 of the 23S rRNA transcripts. Probably also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. HAMAP-Rule MF_00104

Catalytic activity

Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00104

Cofactor

Mg2+ By similarity. HAMAP-Rule MF_00104

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00104

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00104.

Disruption phenotype

Accumulation of full length 23S rRNA as well as larger precursor rRNA transcripts. Strain grows slower than wild-type. Ref.3

Miscellaneous

This organism contains small helical (90-110 nucleotide) intervening sequences (IVSs) in all 7 23S rRNA genes at either the 550-bp or 1170-bp positions or both, accumulating the 23S rRNA as 2.4, 1.7, 1.6, 0.7, and 0.5 kb pieces.

Sequence similarities

Contains 1 DRBM (double-stranded RNA-binding) domain.

Contains 1 RNase III domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 226226Ribonuclease 3 HAMAP-Rule MF_00104
PRO_0000180429

Regions

Domain6 – 128123RNase III
Domain155 – 22571DRBM

Sites

Active site451 Potential
Active site1171 By similarity
Metal binding411Magnesium By similarity
Metal binding1141Magnesium By similarity
Metal binding1171Magnesium By similarity

Experimental info

Sequence conflict57 – 582YH → S in AAA92440. Ref.1
Sequence conflict73 – 742AT → DP in AAA92440. Ref.1
Sequence conflict217 – 2204EQAL → NSV in AAA92440. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q56056 [UniParc].

Last modified January 23, 2002. Version 2.
Checksum: 5752C0113C0A055A

FASTA22625,505
        10         20         30         40         50         60 
MNPIVINRLQ RKLGYTFNHQ ELLQQALTHR SASSKHNERL EFLGDSILSF VIANALYHRF 

        70         80         90        100        110        120 
PRVDEGDMSR MRATLVRGNT LAELAREFDL GECLRLGPGE LKSGGFRRES ILADTVEALI 

       130        140        150        160        170        180 
GGVFLDSNIQ TVEQLILNWY KTRLDEISPG DKQKDPKTRL QEYLQGRHLP LPSYLVVQVR 

       190        200        210        220 
GEAHDQEFTI HCQVSGLSEP VVGTGSSRRK AEQAAAEQAL KKLELE 

« Hide

References

« Hide 'large scale' references
[1]"Structure and regulation of the Salmonella typhimurium rnc-era-recO operon."
Anderson P.E., Matsunaga J., Simons E.L., Simons R.W.
Biochimie 78:1025-1034(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: TSM117.
[2]"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2."
McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. expand/collapse author list , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K.
Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LT2 / SGSC1412 / ATCC 700720.
[3]"RNase III deficient Salmonella typhimurium LT2 contains intervening sequences (IVSs) in its 23S rRNA."
Mattatall N.R., Sanderson K.E.
FEMS Microbiol. Lett. 159:179-185(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U48415 Genomic DNA. Translation: AAA92440.1.
AE006468 Genomic DNA. Translation: AAL21475.1.
RefSeqNP_461516.1. NC_003197.1.

3D structure databases

ProteinModelPortalQ56056.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING99287.STM2581.

Proteomic databases

PRIDEQ56056.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL21475; AAL21475; STM2581.
GeneID1254103.
KEGGstm:STM2581.
PATRIC32383799. VBISalEnt20916_2722.

Phylogenomic databases

HOGENOMHOG000246809.
KOK03685.
OMALTHKSCK.
OrthoDBEOG6T1WVS.
ProtClustDBPRK00102.

Enzyme and pathway databases

BioCycSENT99287:GCTI-2595-MONOMER.

Family and domain databases

Gene3D1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPMF_00104. RNase_III.
InterProIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERPTHR11207. PTHR11207. 1 hit.
PfamPF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view]
SMARTSM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMSSF69065. SSF69065. 1 hit.
TIGRFAMsTIGR02191. RNaseIII. 1 hit.
PROSITEPS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRNC_SALTY
AccessionPrimary (citable) accession number: Q56056
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2002
Last modified: April 16, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families