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Q56056

- RNC_SALTY

UniProt

Q56056 - RNC_SALTY

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Protein
Ribonuclease 3
Gene
rnc, STM2581
Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Digests double-stranded RNA. Involved in the processing of ribosomal RNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Removes small helical intervening sequences (IVSs) from all 7 of the 23S rRNA transcripts. Probably also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Probably processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotation

Cofactori

Mg2+ By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi41 – 411Magnesium By similarity
Active sitei45 – 451 Reviewed prediction
Metal bindingi114 – 1141Magnesium By similarity
Active sitei117 – 1171 By similarity
Metal bindingi117 – 1171Magnesium By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. rRNA binding Source: UniProtKB-KW
  3. ribonuclease III activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA processing Source: UniProtKB-HAMAP
  2. rRNA catabolic process Source: InterPro
  3. rRNA processing Source: UniProtKB-HAMAP
  4. tRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

mRNA processing, rRNA processing, tRNA processing

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciSENT99287:GCTI-2595-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 3 (EC:3.1.26.3)
Alternative name(s):
Ribonuclease III
Short name:
RNase III
Gene namesi
Name:rnc
Ordered Locus Names:STM2581
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Accumulation of full length 23S rRNA as well as larger precursor rRNA transcripts. Strain grows slower than wild-type.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 226226Ribonuclease 3UniRule annotation
PRO_0000180429Add
BLAST

Proteomic databases

PRIDEiQ56056.

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi99287.STM2581.

Structurei

3D structure databases

ProteinModelPortaliQ56056.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 128123RNase III
Add
BLAST
Domaini155 – 22571DRBM
Add
BLAST

Sequence similaritiesi

Contains 1 RNase III domain.

Phylogenomic databases

HOGENOMiHOG000246809.
KOiK03685.
OMAiAQKDPKT.
OrthoDBiEOG6T1WVS.
PhylomeDBiQ56056.

Family and domain databases

Gene3Di1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPiMF_00104. RNase_III.
InterProiIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERiPTHR11207. PTHR11207. 1 hit.
PfamiPF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMiSSF69065. SSF69065. 1 hit.
TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
PROSITEiPS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q56056-1 [UniParc]FASTAAdd to Basket

« Hide

MNPIVINRLQ RKLGYTFNHQ ELLQQALTHR SASSKHNERL EFLGDSILSF    50
VIANALYHRF PRVDEGDMSR MRATLVRGNT LAELAREFDL GECLRLGPGE 100
LKSGGFRRES ILADTVEALI GGVFLDSNIQ TVEQLILNWY KTRLDEISPG 150
DKQKDPKTRL QEYLQGRHLP LPSYLVVQVR GEAHDQEFTI HCQVSGLSEP 200
VVGTGSSRRK AEQAAAEQAL KKLELE 226
Length:226
Mass (Da):25,505
Last modified:January 23, 2002 - v2
Checksum:i5752C0113C0A055A
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti57 – 582YH → S in AAA92440. 1 Publication
Sequence conflicti73 – 742AT → DP in AAA92440. 1 Publication
Sequence conflicti217 – 2204EQAL → NSV in AAA92440. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U48415 Genomic DNA. Translation: AAA92440.1.
AE006468 Genomic DNA. Translation: AAL21475.1.
RefSeqiNP_461516.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL21475; AAL21475; STM2581.
GeneIDi1254103.
KEGGistm:STM2581.
PATRICi32383799. VBISalEnt20916_2722.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U48415 Genomic DNA. Translation: AAA92440.1 .
AE006468 Genomic DNA. Translation: AAL21475.1 .
RefSeqi NP_461516.1. NC_003197.1.

3D structure databases

ProteinModelPortali Q56056.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 99287.STM2581.

Proteomic databases

PRIDEi Q56056.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL21475 ; AAL21475 ; STM2581 .
GeneIDi 1254103.
KEGGi stm:STM2581.
PATRICi 32383799. VBISalEnt20916_2722.

Phylogenomic databases

HOGENOMi HOG000246809.
KOi K03685.
OMAi AQKDPKT.
OrthoDBi EOG6T1WVS.
PhylomeDBi Q56056.

Enzyme and pathway databases

BioCyci SENT99287:GCTI-2595-MONOMER.

Family and domain databases

Gene3Di 1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPi MF_00104. RNase_III.
InterProi IPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view ]
PANTHERi PTHR11207. PTHR11207. 1 hit.
Pfami PF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view ]
SMARTi SM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view ]
SUPFAMi SSF69065. SSF69065. 1 hit.
TIGRFAMsi TIGR02191. RNaseIII. 1 hit.
PROSITEi PS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and regulation of the Salmonella typhimurium rnc-era-recO operon."
    Anderson P.E., Matsunaga J., Simons E.L., Simons R.W.
    Biochimie 78:1025-1034(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: TSM117.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.
  3. "RNase III deficient Salmonella typhimurium LT2 contains intervening sequences (IVSs) in its 23S rRNA."
    Mattatall N.R., Sanderson K.E.
    FEMS Microbiol. Lett. 159:179-185(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
    Strain: LT2 / SGSC1412 / ATCC 700720.

Entry informationi

Entry nameiRNC_SALTY
AccessioniPrimary (citable) accession number: Q56056
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2002
Last modified: September 3, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

This organism contains small helical (90-110 nucleotide) intervening sequences (IVSs) in all 7 23S rRNA genes at either the 550-bp or 1170-bp positions or both, accumulating the 23S rRNA as 2.4, 1.7, 1.6, 0.7, and 0.5 kb pieces.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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