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Q56056

- RNC_SALTY

UniProt

Q56056 - RNC_SALTY

Protein

Ribonuclease 3

Gene

rnc

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 2 (23 Jan 2002)
      Previous versions | rss
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    Functioni

    Digests double-stranded RNA. Involved in the processing of ribosomal RNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Removes small helical intervening sequences (IVSs) from all 7 of the 23S rRNA transcripts. Probably also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Probably processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.

    Catalytic activityi

    Endonucleolytic cleavage to 5'-phosphomonoester.

    Cofactori

    Mg2+.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi41 – 411MagnesiumBy similarity
    Active sitei45 – 451Sequence Analysis
    Metal bindingi114 – 1141MagnesiumBy similarity
    Active sitei117 – 1171By similarity
    Metal bindingi117 – 1171MagnesiumBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. ribonuclease III activity Source: UniProtKB-HAMAP
    3. rRNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. mRNA processing Source: UniProtKB-HAMAP
    2. rRNA catabolic process Source: InterPro
    3. rRNA processing Source: UniProtKB-HAMAP
    4. tRNA processing Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    mRNA processing, rRNA processing, tRNA processing

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding, rRNA-binding

    Enzyme and pathway databases

    BioCyciSENT99287:GCTI-2595-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease 3 (EC:3.1.26.3)
    Alternative name(s):
    Ribonuclease III
    Short name:
    RNase III
    Gene namesi
    Name:rnc
    Ordered Locus Names:STM2581
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
    ProteomesiUP000001014: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Accumulation of full length 23S rRNA as well as larger precursor rRNA transcripts. Strain grows slower than wild-type.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 226226Ribonuclease 3PRO_0000180429Add
    BLAST

    Proteomic databases

    PRIDEiQ56056.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi99287.STM2581.

    Structurei

    3D structure databases

    ProteinModelPortaliQ56056.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini6 – 128123RNase IIIAdd
    BLAST
    Domaini155 – 22571DRBMAdd
    BLAST

    Sequence similaritiesi

    Contains 1 RNase III domain.Curated

    Phylogenomic databases

    HOGENOMiHOG000246809.
    KOiK03685.
    OMAiAQKDPKT.
    OrthoDBiEOG6T1WVS.
    PhylomeDBiQ56056.

    Family and domain databases

    Gene3Di1.10.1520.10. 1 hit.
    3.30.160.20. 1 hit.
    HAMAPiMF_00104. RNase_III.
    InterProiIPR014720. dsRNA-bd_dom.
    IPR011907. RNase_III.
    IPR000999. RNase_III_dom.
    [Graphical view]
    PANTHERiPTHR11207. PTHR11207. 1 hit.
    PfamiPF00035. dsrm. 1 hit.
    PF14622. Ribonucleas_3_3. 1 hit.
    [Graphical view]
    SMARTiSM00358. DSRM. 1 hit.
    SM00535. RIBOc. 1 hit.
    [Graphical view]
    SUPFAMiSSF69065. SSF69065. 1 hit.
    TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
    PROSITEiPS50137. DS_RBD. 1 hit.
    PS00517. RNASE_3_1. 1 hit.
    PS50142. RNASE_3_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q56056-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNPIVINRLQ RKLGYTFNHQ ELLQQALTHR SASSKHNERL EFLGDSILSF    50
    VIANALYHRF PRVDEGDMSR MRATLVRGNT LAELAREFDL GECLRLGPGE 100
    LKSGGFRRES ILADTVEALI GGVFLDSNIQ TVEQLILNWY KTRLDEISPG 150
    DKQKDPKTRL QEYLQGRHLP LPSYLVVQVR GEAHDQEFTI HCQVSGLSEP 200
    VVGTGSSRRK AEQAAAEQAL KKLELE 226
    Length:226
    Mass (Da):25,505
    Last modified:January 23, 2002 - v2
    Checksum:i5752C0113C0A055A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti57 – 582YH → S in AAA92440. (PubMed:9150881)Curated
    Sequence conflicti73 – 742AT → DP in AAA92440. (PubMed:9150881)Curated
    Sequence conflicti217 – 2204EQAL → NSV in AAA92440. (PubMed:9150881)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U48415 Genomic DNA. Translation: AAA92440.1.
    AE006468 Genomic DNA. Translation: AAL21475.1.
    RefSeqiNP_461516.1. NC_003197.1.

    Genome annotation databases

    EnsemblBacteriaiAAL21475; AAL21475; STM2581.
    GeneIDi1254103.
    KEGGistm:STM2581.
    PATRICi32383799. VBISalEnt20916_2722.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U48415 Genomic DNA. Translation: AAA92440.1 .
    AE006468 Genomic DNA. Translation: AAL21475.1 .
    RefSeqi NP_461516.1. NC_003197.1.

    3D structure databases

    ProteinModelPortali Q56056.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 99287.STM2581.

    Proteomic databases

    PRIDEi Q56056.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL21475 ; AAL21475 ; STM2581 .
    GeneIDi 1254103.
    KEGGi stm:STM2581.
    PATRICi 32383799. VBISalEnt20916_2722.

    Phylogenomic databases

    HOGENOMi HOG000246809.
    KOi K03685.
    OMAi AQKDPKT.
    OrthoDBi EOG6T1WVS.
    PhylomeDBi Q56056.

    Enzyme and pathway databases

    BioCyci SENT99287:GCTI-2595-MONOMER.

    Family and domain databases

    Gene3Di 1.10.1520.10. 1 hit.
    3.30.160.20. 1 hit.
    HAMAPi MF_00104. RNase_III.
    InterProi IPR014720. dsRNA-bd_dom.
    IPR011907. RNase_III.
    IPR000999. RNase_III_dom.
    [Graphical view ]
    PANTHERi PTHR11207. PTHR11207. 1 hit.
    Pfami PF00035. dsrm. 1 hit.
    PF14622. Ribonucleas_3_3. 1 hit.
    [Graphical view ]
    SMARTi SM00358. DSRM. 1 hit.
    SM00535. RIBOc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF69065. SSF69065. 1 hit.
    TIGRFAMsi TIGR02191. RNaseIII. 1 hit.
    PROSITEi PS50137. DS_RBD. 1 hit.
    PS00517. RNASE_3_1. 1 hit.
    PS50142. RNASE_3_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and regulation of the Salmonella typhimurium rnc-era-recO operon."
      Anderson P.E., Matsunaga J., Simons E.L., Simons R.W.
      Biochimie 78:1025-1034(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: TSM117.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LT2 / SGSC1412 / ATCC 700720.
    3. "RNase III deficient Salmonella typhimurium LT2 contains intervening sequences (IVSs) in its 23S rRNA."
      Mattatall N.R., Sanderson K.E.
      FEMS Microbiol. Lett. 159:179-185(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
      Strain: LT2 / SGSC1412 / ATCC 700720.

    Entry informationi

    Entry nameiRNC_SALTY
    AccessioniPrimary (citable) accession number: Q56056
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: January 23, 2002
    Last modified: October 1, 2014
    This is version 102 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This organism contains small helical (90-110 nucleotide) intervening sequences (IVSs) in all 7 23S rRNA genes at either the 550-bp or 1170-bp positions or both, accumulating the 23S rRNA as 2.4, 1.7, 1.6, 0.7, and 0.5 kb pieces.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3