Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cell invasion protein SipB

Gene

sipB

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for entry into the host cell through presentation or delivery of SipC at the host cell plasma membrane. Along with SipC, is necessary for the transfer of other effector proteins into the host cell. Induces macrophage apoptosis either by binding and activating the proapoptotic enzyme caspase-1 (caspase-1 dependent), resulting in the release of interleukin-1 beta active form, or by disrupting mitochondria and inducing autophagy (caspase-1 independent). The former is dependent of its membrane-fusion activity. The SipBC complex, in association with its chaperone SicA, is regulated by binding of InvE.2 Publications

GO - Biological processi

  • establishment of protein localization to Golgi Source: AgBase
  • pathogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Virulence

Enzyme and pathway databases

BioCyciSENT99287:GCTI-2901-MONOMER.

Protein family/group databases

TCDBi1.C.36.3.2. the bacterial type iii-target cell pore (iiitcp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell invasion protein SipB
Alternative name(s):
Effector protein SipB
Gene namesi
Name:sipB
Synonyms:sspB
Ordered Locus Names:STM2885
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

  • Secreted 1 Publication
  • Host cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

  • Note: Secreted via the type III secretion system 1 (SPI-1 TTSS), inserted into the phagosomal membrane and subsequently released into the host cell cytoplasm.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei320 – 35435HelicalAdd
BLAST
Transmembranei408 – 42720HelicalAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 593593Cell invasion protein SipBPRO_0000219856Add
BLAST

Proteomic databases

PaxDbiQ56019.
PRIDEiQ56019.

Interactioni

Subunit structurei

Homotrimer.1 Publication

Protein-protein interaction databases

STRINGi99287.STM2885.

Structurei

Secondary structure

1
593
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi85 – 9915Combined sources
Helixi101 – 11818Combined sources
Turni119 – 1224Combined sources
Helixi129 – 17143Combined sources
Helixi183 – 22139Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3TULX-ray2.79A/B/C/D81-237[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili151 – 20858Sequence analysisAdd
BLAST
Coiled coili287 – 31428Sequence analysisAdd
BLAST

Domaini

SipB membrane integration requires both hydrophobic domains and the helical C-terminal region.

Sequence similaritiesi

Belongs to the invasin protein B family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4106H1J. Bacteria.
ENOG410XWFQ. LUCA.
HOGENOMiHOG000028231.
KOiK13285.
OMAiRNATATF.
OrthoDBiEOG6WHNNM.

Family and domain databases

InterProiIPR032391. SipB_N.
IPR006972. SseC.
IPR003895. T3SS_invasion_prot_B.
[Graphical view]
PfamiPF04888. SseC. 1 hit.
PF16535. T3SSipB. 1 hit.
[Graphical view]
PRINTSiPR01375. BACINVASINB.

Sequencei

Sequence statusi: Complete.

Q56019-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNDASSISR SGYTQNPRLA EAAFEGVRKN TDFLKAADKA FKDVVATKAG
60 70 80 90 100
DLKAGTKSGE SAINTVGLKP PTDAAREKLS SEGQLTLLLG KLMTLLGDVS
110 120 130 140 150
LSQLESRLAV WQAMIESQKE MGIQVSKEFQ TALGEAQEAT DLYEASIKKT
160 170 180 190 200
DTAKSVYDAA TKKLTQAQNK LQSLDPADPG YAQAEAAVEQ AGKEATEAKE
210 220 230 240 250
ALDKATDATV KAGTDAKAKA EKADNILTKF QGTANAASQN QVSQGEQDNL
260 270 280 290 300
SNVARLTMLM AMFIEIVGKN TEESLQNDLA LFNALQEGRQ AEMEKKSAEF
310 320 330 340 350
QEETRKAEET NRIMGCIGKV LGALLTIVSV VAAVFTGGAS LALAAVGLAV
360 370 380 390 400
MVADEIVKAA TGVSFIQQAL NPIMEHVLKP LMELIGKAIT KALEGLGVDK
410 420 430 440 450
KTAEMAGSIV GAIVAAIAMV AVIVVVAVVG KGAAAKLGNA LSKMMGETIK
460 470 480 490 500
KLVPNVLKQL AQNGSKLFTQ GMQRITSGLG NVGSKMGLQT NALSKELVGN
510 520 530 540 550
TLNKVALGME VTNTAAQSAG GVAEGVFIKN ASEALADFML ARFAMDQIQQ
560 570 580 590
WLKQSVEIFG ENQKVTAELQ KAMSSAVQQN ADASRFILRQ SRA
Length:593
Mass (Da):62,451
Last modified:November 1, 1996 - v1
Checksum:i63A5D5949D85BA46
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25631 Genomic DNA. Translation: AAA75169.1.
AE006468 Genomic DNA. Translation: AAL21765.1.
U30491 Genomic DNA. Translation: AAC43546.1.
PIRiS70547.
RefSeqiNP_461806.1. NC_003197.1.
WP_000245788.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL21765; AAL21765; STM2885.
GeneIDi1254408.
KEGGistm:STM2885.
PATRICi32384458. VBISalEnt20916_3041.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25631 Genomic DNA. Translation: AAA75169.1.
AE006468 Genomic DNA. Translation: AAL21765.1.
U30491 Genomic DNA. Translation: AAC43546.1.
PIRiS70547.
RefSeqiNP_461806.1. NC_003197.1.
WP_000245788.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3TULX-ray2.79A/B/C/D81-237[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM2885.

Protein family/group databases

TCDBi1.C.36.3.2. the bacterial type iii-target cell pore (iiitcp) family.

Proteomic databases

PaxDbiQ56019.
PRIDEiQ56019.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL21765; AAL21765; STM2885.
GeneIDi1254408.
KEGGistm:STM2885.
PATRICi32384458. VBISalEnt20916_3041.

Phylogenomic databases

eggNOGiENOG4106H1J. Bacteria.
ENOG410XWFQ. LUCA.
HOGENOMiHOG000028231.
KOiK13285.
OMAiRNATATF.
OrthoDBiEOG6WHNNM.

Enzyme and pathway databases

BioCyciSENT99287:GCTI-2901-MONOMER.

Family and domain databases

InterProiIPR032391. SipB_N.
IPR006972. SseC.
IPR003895. T3SS_invasion_prot_B.
[Graphical view]
PfamiPF04888. SseC. 1 hit.
PF16535. T3SSipB. 1 hit.
[Graphical view]
PRINTSiPR01375. BACINVASINB.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Homologs of the Shigella IpaB and IpaC invasins are required for Salmonella typhimurium entry into cultured epithelial cells."
    Kaniga K., Tucker S.C., Trollinger D., Galan J.E.
    J. Bacteriol. 177:3965-3971(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SL1344.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.
  3. "Salmonella typhimurium secreted invasion determinants are homologous to Shigella Ipa proteins."
    Hueck C.J., Hantman M.J., Bajaj V., Johnston C., Lee C.A., Miller S.I.
    Mol. Microbiol. 18:479-490(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 305-593.
    Strain: SL1344.
  4. "The Salmonella invasin SipB induces macrophage apoptosis by binding to caspase-1."
    Hersh D., Monack D.M., Smith M.R., Ghori N., Falkow S., Zychlinsky A.
    Proc. Natl. Acad. Sci. U.S.A. 96:2396-2401(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN CASPASE-1-DEPENDENT APOPTOSIS.
    Strain: C5 and SL1344.
  5. "Membrane fusion activity of purified SipB, a Salmonella surface protein essential for mammalian cell invasion."
    Hayward R.D., McGhie E.J., Koronakis V.
    Mol. Microbiol. 37:727-739(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, MEMBRANE-FUSION ACTIVITY.
    Strain: SJW1103.
  6. "The Salmonella type III secretion translocon protein SspC is inserted into the epithelial cell plasma membrane upon infection."
    Scherer C.A., Cooper E., Miller S.I.
    Mol. Microbiol. 37:1133-1145(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: ATCC 14028s / SGSG 2262.
  7. "Salmonella type III secretion-associated protein InvE controls translocation of effector proteins into host cells."
    Kubori T., Galan J.E.
    J. Bacteriol. 184:4699-4708(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: REGULATION BY INVE.
  8. "Topology of the Salmonella invasion protein SipB in a model bilayer."
    McGhie E.J., Hume P.J., Hayward R.D., Torres J., Koronakis V.
    Mol. Microbiol. 44:1309-1321(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.
    Strain: SJW1103.
  9. "A Salmonella protein causes macrophage cell death by inducing autophagy."
    Hernandez L.D., Pypaert M., Flavell R.A., Galan J.E.
    J. Cell Biol. 163:1123-1131(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN CASPASE-1-INDEPENDENT APOPTOSIS.
    Strain: SL1344.

Entry informationi

Entry nameiSIPB_SALTY
AccessioniPrimary (citable) accession number: Q56019
Secondary accession number(s): Q56024, Q7CPX0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.