Reviewed, UniProtKB/Swiss-Prot Q55W30 (KMO_CRYNE)

Names and origin

Protein names

Recommended name:
    Kynurenine 3-monooxygenase
    EC=1.14.13.9
Alternative name(s):
    Kynurenine 3-hydroxylase
    Biosynthesis of nicotinic acid protein 4

Gene names

Name:	BNA4
Ordered Locus Names:	CNC04120, CNBC3070

Organism

Cryptococcus neoformans (Filobasidiella neoformans) [Complete proteome]

Taxonomic identifier

5207 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Tremellomycetes › Tremellales › Tremellaceae › Filobasidiella › Filobasidiella/Cryptococcus neoformans species complex

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid By similarity.
Catalytic activity	L-kynurenine + NADPH + O2 = 3-hydroxy-L-kynurenine + NADP+ + H2O.
Cofactor	FAD By similarity.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 1/3.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the aromatic-ring hydroxylase family. KMO subfamily.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Mitochondrion
Ligand	FAD Flavoprotein NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pyridine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kynurenine 3-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 506	506	Kynurenine 3-monooxygenase		PRO_0000361926
Natural variations
	Natural variant	224	1	F → S in strain: B-3501A.

Sequences

	Sequence		Length	Mass (Da)
	Q55W30-1 [UniParc]. Last modified February 10, 2009. Version 2. Checksum: 997F7366D265633C	FASTA	506	56,539
	10 20 30 40 50 60 MSQSRARKVL IVGAGPVGAL TALSLHRRGW EVEVWETRDD PRGQDAAPSN LRSINLAISS 70 80 90 100 110 120 RGLEALRSVD PSIAENFLEE AIPMKGRMIH HTDGKQESQL YDPIGGQSIN SISRPILNQR 130 140 150 160 170 180 LVQSLPEAVK LRFNTKLKHI DFKNRVAYAS HKQETSLLPG EESEKDKKQN TEDEDGTAFD 190 200 210 220 230 240 LVIGCDGSWS KVRTAMMRAE RIDFSQSFIP HAYIELHMPS DPAFPGGYAM DKNHLHIWPR 250 260 270 280 290 300 HAFMLIGLPN KDGSFTLTLF IPFSSLELLD TRESAAAFFR EHFPSAVDIV GEKVLLDDFE 310 320 330 340 350 360 KNPRGNLVTI NCTPSAWSSH AILLGDASHS MVPFYGQGLN CGLEDVRVLS SILERHHISP 370 380 390 400 410 420 TTTLALGETD PELELALKAY SDERQGDLKA ICELALQNYT EMRSHVLSPL HHLRRQVDKV 430 440 450 460 470 480 FTTLFRSAPQ ATLSLMEPFP TKKVRGWTSL YEMVTFRPDV GYSEALRKER WQKDVVGYAG 490 500 WIGGVVGVGA AGVFAATMAK KWLERR

References

[1]

"The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans." Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D., Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E., Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A., Fox D.S. , Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C., Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J., Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A., Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E., Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A., Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W., Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W. Science 307:1321-1324(2005) [PubMed: 15653466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B-3501A and JEC21.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017343 Genomic DNA. Translation: AAW42348.1. AAEY01000013 Genomic DNA. Translation: EAL22169.1.
RefSeq	XP_569655.1. XP_776816.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q55W30.
Genome annotation databases
GeneID	3256682. 4934972.
GenomeReviews	Gene locus CNC04120 in contig AE017343_GR. Gene locus CNBC3070 in contig CM000042_GR.
KEGG	cnb:CNBC3070. cne:CNC04120.
Phylogenomic databases
OMA	LHAIMPS.
Family and domain databases
InterPro	IPR006076. FAD-dep_OxRdtase. IPR003042. Rng_hydrolase-like. [Graphical view]
Pfam	PF01266. DAO. 1 hit. [Graphical view]
PRINTS	PR00420. RNGMNOXGNASE.
ProtoNet	Search...

Entry information

Entry name

KMO_CRYNE

Accession

Primary (citable) accession number: Q55W30
Secondary accession number(s): Q5KK63

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	February 10, 2009
Last modified:	November 3, 2009
This is version 26 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)