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Protein

DNA mismatch repair protein Msh6

Gene

msh6

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with msh2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DDI-5358565. Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).

Names & Taxonomyi

Protein namesi
Recommended name:
DNA mismatch repair protein Msh6
Gene namesi
Name:msh6
ORF Names:DDB_G0268614
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 1, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0268614. msh6.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003285611 – 1260DNA mismatch repair protein Msh6Add BLAST1260

Proteomic databases

PaxDbiQ55GU9.
PRIDEiQ55GU9.

Interactioni

Subunit structurei

Heterodimer consisting of msh2-msh6 (MutS alpha). Forms a ternary complex with MutL alpha (mlh1-pms1). Interacts with exo1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi44689.DDB0229918.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi76 – 83Poly-Thr8
Compositional biasi139 – 148Poly-Gln10
Compositional biasi239 – 244Poly-Ser6
Compositional biasi247 – 252Poly-Thr6
Compositional biasi276 – 282Poly-Asp7
Compositional biasi285 – 296Poly-AspAdd BLAST12
Compositional biasi795 – 801Poly-Asn7

Sequence similaritiesi

Belongs to the DNA mismatch repair MutS family.Curated

Phylogenomic databases

eggNOGiKOG0217. Eukaryota.
COG0249. LUCA.
InParanoidiQ55GU9.
KOiK08737.
OMAiHMACAFK.
PhylomeDBiQ55GU9.

Family and domain databases

Gene3Di3.30.420.110. 1 hit.
3.40.1170.10. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR007695. DNA_mismatch_repair_MutS-lik_N.
IPR000432. DNA_mismatch_repair_MutS_C.
IPR007861. DNA_mismatch_repair_MutS_clamp.
IPR007696. DNA_mismatch_repair_MutS_core.
IPR016151. DNA_mismatch_repair_MutS_N.
IPR007860. DNA_mmatch_repair_MutS_con_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01624. MutS_I. 1 hit.
PF05188. MutS_II. 1 hit.
PF05192. MutS_III. 1 hit.
PF05190. MutS_IV. 1 hit.
PF00488. MutS_V. 1 hit.
[Graphical view]
SMARTiSM00534. MUTSac. 1 hit.
SM00533. MUTSd. 1 hit.
[Graphical view]
SUPFAMiSSF48334. SSF48334. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53150. SSF53150. 1 hit.
SSF55271. SSF55271. 1 hit.
PROSITEiPS00486. DNA_MISMATCH_REPAIR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q55GU9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPNNMKQTN ISQFFAPTKQ PEPIIEEGFD SFFDDIPTEE LEPDLNSIPI
60 70 80 90 100
SSSQQTSTSN TSSQQTSSAN TSSSATTTTT TNTNSQIPKA PTTPSKLKLP
110 120 130 140 150
TSFTSKSNTP TKSLPPSNFS LVVNEQEEEN EMNDNSKPQQ QQQQQQQQNL
160 170 180 190 200
FKSNITSNSG GSSNDLFSRF KDEKPKTTTT LSTPSKPLSQ PQRNNTNEIS
210 220 230 240 250
KKRKEMDFNE DESNTNYDNN NNDVEDLDEF CFVNKNKPSS SSSSLNTNTT
260 270 280 290 300
TTPKAPTITP KTPTKTPTRR PVVTYDEDDD DDEQDDDEDD DDDDDDKKSK
310 320 330 340 350
STTTTAVKKK GNAFGKKDKK EIEERYSFLV NIKDANGNPK DHPDYDKRTL
360 370 380 390 400
HIPASCLSKF SPFERQFWDI KSKNYDTVVF FKKGKFYELY ESDADIGHQQ
410 420 430 440 450
LHLKLTDRVN MRMVGVPEMS FNHWASKLIH LGHKVAKVDQ METSIGMAKR
460 470 480 490 500
QNEKGGRNKK DSIIQRELTS ILTAGTLLDE QMITDQTSTY LMAIKENEYD
510 520 530 540 550
KQYGVCFVDV SIGEFYLCTI QDDDNRMQFE TLLLQMMPKE IVYEKGATSP
560 570 580 590 600
KTISIMKRVL STVKPVMNAR LSLEYWDPTD TMERITQLCG GKTPETLCQM
610 620 630 640 650
KNEEYLMGAL GGCISYLMDI KIGNSVVEQA RFKRFNPLDI GNSMILDGQC
660 670 680 690 700
LVNLEIFNNS TDGSTEGTLF KLMDRCTTAF GKRMFRQWIC RPLANKNAIV
710 720 730 740 750
DRQKAIEFLR DSPETLQKVT AILNKLPDLE RMIARIRAQT SKISDLISVL
760 770 780 790 800
NHFDNIHSKL LELLDEAEQI ESIHLRSCLF MDNQQDNDDI DEQENSNNNN
810 820 830 840 850
NIRYSGYPNL KPYIERVRKS FTIEQDRVVP SKGLFLEFDQ CLGNIQSLEQ
860 870 880 890 900
SFAKHLEEQK AHFKCNKIEY KHMGKEIYQI EIPVAFTKKL PAGFSLKSSS
910 920 930 940 950
SKVNRYHSPF VTKNLTSLLE ERDTYEVLSK EVLKKILSNF AIYFNHFQIA
960 970 980 990 1000
ITKLSQLDCL LSLYKVSFQS SIQMCRPLFV SSDQRGFIDV KDMRHPCIYS
1010 1020 1030 1040 1050
KSGDDFIPND ISLNTENNPP SLMVLTGPNM GGKSTLLRQS CILVIMAQMG
1060 1070 1080 1090 1100
CYVSASSCEM SIVDRIFTRL GANDNILAGQ STFMVELAET SAVLKYATKR
1110 1120 1130 1140 1150
SLVILDELGR GTSTFDGYSI AYSVLNYLAT KVQSMCIFAT HYQSLAYEPT
1160 1170 1180 1190 1200
VRDLISTAYM TCHVDEEAKK VIFLYKLASG VCPNSYGLHV ASMAGLPREI
1210 1220 1230 1240 1250
ITKAEEKSTQ MEKDSVLVSF IHGTISRSKL VEKIVQSYKQ KDLNQLIQLS
1260
NTLKDLNLNK
Length:1,260
Mass (Da):142,747
Last modified:May 24, 2005 - v1
Checksum:i4A3DEA3D20B03584
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000003 Genomic DNA. Translation: EAL73760.1.
RefSeqiXP_647085.1. XM_641993.1.

Genome annotation databases

EnsemblProtistsiEAL73760; EAL73760; DDB_G0268614.
GeneIDi8615889.
KEGGiddi:DDB_G0268614.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000003 Genomic DNA. Translation: EAL73760.1.
RefSeqiXP_647085.1. XM_641993.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0229918.

Proteomic databases

PaxDbiQ55GU9.
PRIDEiQ55GU9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL73760; EAL73760; DDB_G0268614.
GeneIDi8615889.
KEGGiddi:DDB_G0268614.

Organism-specific databases

dictyBaseiDDB_G0268614. msh6.

Phylogenomic databases

eggNOGiKOG0217. Eukaryota.
COG0249. LUCA.
InParanoidiQ55GU9.
KOiK08737.
OMAiHMACAFK.
PhylomeDBiQ55GU9.

Enzyme and pathway databases

ReactomeiR-DDI-5358565. Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).

Miscellaneous databases

PROiQ55GU9.

Family and domain databases

Gene3Di3.30.420.110. 1 hit.
3.40.1170.10. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR007695. DNA_mismatch_repair_MutS-lik_N.
IPR000432. DNA_mismatch_repair_MutS_C.
IPR007861. DNA_mismatch_repair_MutS_clamp.
IPR007696. DNA_mismatch_repair_MutS_core.
IPR016151. DNA_mismatch_repair_MutS_N.
IPR007860. DNA_mmatch_repair_MutS_con_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01624. MutS_I. 1 hit.
PF05188. MutS_II. 1 hit.
PF05192. MutS_III. 1 hit.
PF05190. MutS_IV. 1 hit.
PF00488. MutS_V. 1 hit.
[Graphical view]
SMARTiSM00534. MUTSac. 1 hit.
SM00533. MUTSd. 1 hit.
[Graphical view]
SUPFAMiSSF48334. SSF48334. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53150. SSF53150. 1 hit.
SSF55271. SSF55271. 1 hit.
PROSITEiPS00486. DNA_MISMATCH_REPAIR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMSH6_DICDI
AccessioniPrimary (citable) accession number: Q55GU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: November 2, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.