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Q55GJ6 (PSB6_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proteasome subunit beta type-6

EC=3.4.25.1
Alternative name(s):
Differentiation-associated proteasome subunit 1
Short name=DAPS-1
Gene names
Name:psmB6
Synonyms:dapA
ORF Names:DDB_G0267390
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length214 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Ref.3

Catalytic activity

Cleavage of peptide bonds with very broad specificity.

Subunit structure

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel By similarity.

Subcellular location

Cytoplasm. Nucleus Ref.3.

Developmental stage

Maximally expressed 4-6 hours after starvation (around the aggregation-stream stage), followed by a drastic decrease from the mound to tipped aggregate stage. Ref.1

Sequence similarities

Belongs to the peptidase T1B family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 1414Removed in mature form
PRO_0000327384
Chain15 – 214200Proteasome subunit beta type-6
PRO_0000327385

Sites

Active site151Nucleophile By similarity

Experimental info

Sequence conflict251G → R AA sequence Ref.3
Sequence conflict281M → A AA sequence Ref.3
Sequence conflict951S → C in BAA25923. Ref.1
Sequence conflict1221Missing in BAA25923. Ref.1
Sequence conflict1651E → R in BAA25923. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q55GJ6 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: 1EA0E6921D37AAE2

FASTA21423,446
        10         20         30         40         50         60 
MEAPEWLDNA VDLGTSIMAV EYDGGVIMGA DSRTTTGAYI ANRVTNKITP IHERIYCCRS 

        70         80         90        100        110        120 
GSAADTQAIS DYVRYYLEMH TSELCDEPDV KTAASLFQLL CYSNKNNLMA GIIVAGWDKH 

       130        140        150        160        170        180 
QGGSVYNISL GGSMVKQPFA IGGSGSTYIY GYCDSKFKPK MTKDECIEFV QNSLALAMFR 

       190        200        210 
DGSSGGVIRL CIIDKNGVER KMIPGNNLPR FWEG 

« Hide

References

« Hide 'large scale' references
[1]"Preferential expression of the cDNA encoding the proteasome subunit during the growth/differentiation transition of Dictyostelium cells."
Chae S.-C., Maeda Y.
Biochem. Biophys. Res. Commun. 245:231-234(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
Strain: AX2.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[3]"Proteasomes from Dictyostelium discoideum: characterization of structure and function."
Schauer T.M., Nesper M., Kehl M., Lottspeich F., Mueller-Taubenberger A., Gerisch G., Baumeister W.
J. Struct. Biol. 111:135-147(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 15-32, FUNCTION, SUBCELLULAR LOCATION.
Strain: AX2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB007024 mRNA. Translation: BAA25923.1.
AAFI02000003 Genomic DNA. Translation: EAL73147.1.
PIRJE0101.
RefSeqXP_647188.1. XM_642096.1.

3D structure databases

ProteinModelPortalQ55GJ6.
SMRQ55GJ6. Positions 15-211.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0191199.

Protein family/group databases

MEROPST01.010.

Proteomic databases

PRIDEQ55GJ6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0191199; DDB0191199; DDB_G0267390.
GeneID8615992.
KEGGddi:DDB_G0267390.

Organism-specific databases

dictyBaseDDB_G0267390. psmB6.

Phylogenomic databases

eggNOGCOG0638.
OMATSIMAVQ.
PhylomeDBQ55GJ6.

Family and domain databases

Gene3D3.60.20.10. 1 hit.
InterProIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSPR00141. PROTEASOME.
SUPFAMSSF56235. SSF56235. 1 hit.
PROSITEPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ55GJ6.

Entry information

Entry namePSB6_DICDI
AccessionPrimary (citable) accession number: Q55GJ6
Secondary accession number(s): O60953
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: July 9, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase