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Protein

Proteasome subunit beta type-6

Gene

psmB6

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.1 Publication

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei15NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

  • proteolysis Source: dictyBase
  • proteolysis involved in cellular protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiR-DDI-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-DDI-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-DDI-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-DDI-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-DDI-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-DDI-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-DDI-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-DDI-5632684. Hedgehog 'on' state.
R-DDI-5658442. Regulation of RAS by GAPs.
R-DDI-5689603. UCH proteinases.
R-DDI-5689880. Ub-specific processing proteases.
R-DDI-68949. Orc1 removal from chromatin.
R-DDI-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-DDI-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-DDI-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-DDI-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Protein family/group databases

MEROPSiT01.010.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-6 (EC:3.4.25.1)
Alternative name(s):
Differentiation-associated proteasome subunit 1
Short name:
DAPS-1
Gene namesi
Name:psmB6
Synonyms:dapA
ORF Names:DDB_G0267390
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 1, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0267390. psmB6.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: dictyBase
  • nucleus Source: dictyBase
  • proteasome core complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00003273841 – 14Removed in mature form1 PublicationAdd BLAST14
ChainiPRO_000032738515 – 214Proteasome subunit beta type-6Add BLAST200

Keywords - PTMi

Zymogen

Proteomic databases

PaxDbiQ55GJ6.
PRIDEiQ55GJ6.

Expressioni

Developmental stagei

Maximally expressed 4-6 hours after starvation (around the aggregation-stream stage), followed by a drastic decrease from the mound to tipped aggregate stage.1 Publication

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel (By similarity).By similarity

Protein-protein interaction databases

STRINGi44689.DDB0191199.

Structurei

3D structure databases

ProteinModelPortaliQ55GJ6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0174. Eukaryota.
ENOG410XS23. LUCA.
InParanoidiQ55GJ6.
KOiK02738.
OMAiTSIMAVQ.
PhylomeDBiQ55GJ6.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q55GJ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAPEWLDNA VDLGTSIMAV EYDGGVIMGA DSRTTTGAYI ANRVTNKITP
60 70 80 90 100
IHERIYCCRS GSAADTQAIS DYVRYYLEMH TSELCDEPDV KTAASLFQLL
110 120 130 140 150
CYSNKNNLMA GIIVAGWDKH QGGSVYNISL GGSMVKQPFA IGGSGSTYIY
160 170 180 190 200
GYCDSKFKPK MTKDECIEFV QNSLALAMFR DGSSGGVIRL CIIDKNGVER
210
KMIPGNNLPR FWEG
Length:214
Mass (Da):23,446
Last modified:May 24, 2005 - v1
Checksum:i1EA0E6921D37AAE2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25G → R AA sequence (PubMed:8130037).Curated1
Sequence conflicti28M → A AA sequence (PubMed:8130037).Curated1
Sequence conflicti95S → C in BAA25923 (PubMed:9535814).Curated1
Sequence conflicti122Missing in BAA25923 (PubMed:9535814).Curated1
Sequence conflicti165E → R in BAA25923 (PubMed:9535814).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007024 mRNA. Translation: BAA25923.1.
AAFI02000003 Genomic DNA. Translation: EAL73147.1.
PIRiJE0101.
RefSeqiXP_647188.1. XM_642096.1.

Genome annotation databases

EnsemblProtistsiEAL73147; EAL73147; DDB_G0267390.
GeneIDi8615992.
KEGGiddi:DDB_G0267390.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007024 mRNA. Translation: BAA25923.1.
AAFI02000003 Genomic DNA. Translation: EAL73147.1.
PIRiJE0101.
RefSeqiXP_647188.1. XM_642096.1.

3D structure databases

ProteinModelPortaliQ55GJ6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0191199.

Protein family/group databases

MEROPSiT01.010.

Proteomic databases

PaxDbiQ55GJ6.
PRIDEiQ55GJ6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL73147; EAL73147; DDB_G0267390.
GeneIDi8615992.
KEGGiddi:DDB_G0267390.

Organism-specific databases

dictyBaseiDDB_G0267390. psmB6.

Phylogenomic databases

eggNOGiKOG0174. Eukaryota.
ENOG410XS23. LUCA.
InParanoidiQ55GJ6.
KOiK02738.
OMAiTSIMAVQ.
PhylomeDBiQ55GJ6.

Enzyme and pathway databases

ReactomeiR-DDI-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-DDI-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-DDI-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-DDI-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-DDI-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-DDI-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-DDI-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-DDI-5632684. Hedgehog 'on' state.
R-DDI-5658442. Regulation of RAS by GAPs.
R-DDI-5689603. UCH proteinases.
R-DDI-5689880. Ub-specific processing proteases.
R-DDI-68949. Orc1 removal from chromatin.
R-DDI-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-DDI-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-DDI-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-DDI-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

PROiQ55GJ6.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSB6_DICDI
AccessioniPrimary (citable) accession number: Q55GJ6
Secondary accession number(s): O60953
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: November 30, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.