Reviewed, UniProtKB/Swiss-Prot Q55G11 (NCSEB_DICDI)

Names and origin

Protein names

Recommended name:
    Neutral ceramidase B
      Short name=N-CDase B
      Short name=NCDase B
    EC=3.5.1.23
Alternative name(s):
    Acylsphingosine deacylase 2B
    N-acylsphingosine amidohydrolase 2B

Gene names

Name:	dcd2B
ORF Names:	DDB_G0268374

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	718 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid By similarity.
Catalytic activity	N-acylsphingosine + H2O = a carboxylate + sphingosine.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the neutral ceramidase family.

Ontologies

Keywords
Biological process	Lipid metabolism Sphingolipid metabolism
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sphingolipid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ceramidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 718	698	Neutral ceramidase B		PRO_0000247109
Sites
	Active site	298	1	Nucleophile By similarity
Amino acid modifications
	Glycosylation	224	1	N-linked (GlcNAc...) Potential
	Glycosylation	252	1	N-linked (GlcNAc...) Potential
	Glycosylation	358	1	N-linked (GlcNAc...) Potential
	Glycosylation	378	1	N-linked (GlcNAc...) Potential
	Glycosylation	391	1	N-linked (GlcNAc...) Potential
	Glycosylation	421	1	N-linked (GlcNAc...) Potential
	Glycosylation	422	1	N-linked (GlcNAc...) Potential
	Glycosylation	577	1	N-linked (GlcNAc...) Potential
	Glycosylation	610	1	N-linked (GlcNAc...) Potential
	Glycosylation	614	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q55G11-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: BDAE0B6C79271AFA	FASTA	718	78,609
	10 20 30 40 50 60 MINSFKKLII LISLVIILLS SNNIFIDSFK IPVNQKNVKS SGDSSYQIGA GIYDITGASA 70 80 90 100 110 120 EVNLMGYANP LQVGAGIHFR QRARAFVFVD SNGNRAVYVS TDSCMIFQEV KIHVVELLQD 130 140 150 160 170 180 IFGPNVYTEA NVLLSGTHTH SGPAGFSQYA LYGITSLGFY KKNFDTICNG IVQAIVKAHK 190 200 210 220 230 240 SVQPANMFTE TGELWNTNIN RSPFAYDNNP EEEKAMYDSN VDKNMTVLRI EDMNGNPFAA 250 260 270 280 290 300 ISFFAVHCTS MNNTNHLISG DNKGYASYLW EKQVNGPGTA GKGPFVAAFG QSNEGDVSPN 310 320 330 340 350 360 TRGPTCRDGS PCDYKTSTCN GRNEECWSLG PGKDGDMFES TQIIGGNQFN KALELFNNAS 370 380 390 400 410 420 IQVSGPVQYR HSWVQFTNVS VEPPYNSGVD NATTCRGAMG YSFAAGTTDG PGAFNFVQSD 430 440 450 460 470 480 NNTSGNPFWN FIGDFIAKPT PDQIRCQSPK PILLDVGMVE PIPWVPDVMP IQIVTIGQIV 490 500 510 520 530 540 LVAVPGEFTT MSGRRLRNSV REIIGESIEN PIVLIAGLSN TYSGYIATFE EYQVQRYEGA 550 560 570 580 590 600 STVFGPHTLG SYMQEFGKLA QSIVDGTTVP AGPTPRNLTG HTLFFLPPVI VDAAPDFDDF 610 620 630 640 650 660 GEVSIDVNLN YSVNETVSCV FYGGNPRNDF MIESSFLSVD LLTGTDQWTT VLDDGDWDTK 670 680 690 700 710 FKWKMHDLGF SLITIEWVIA PDTTPGTYRI THSGFAKKNP FSSNLTPYQG ISRNFVVQ

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000003 Genomic DNA. Translation: EAL73640.1.
RefSeq	XP_647372.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3398682.
GenomeReviews	Gene locus dcd2B in contig CM000150_GR.
KEGG	ddi:DDB_0232168.
Organism-specific databases
dictyBase	DDB_G0268374. dcd2B.
Phylogenomic databases
OMA	SWFAVHP.
Enzyme and pathway databases
BRENDA	3.5.1.23. 424.
Family and domain databases
InterPro	IPR006823. Ceramidase_alk. [Graphical view]
PANTHER	PTHR12670. Ceramidase_alk. 1 hit.
Pfam	PF04734. Ceramidase_alk. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NCSEB_DICDI

Accession

Primary (citable) accession number: Q55G11

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	May 24, 2005
Last modified:	November 3, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)