Neutral ceramidase B precursor - Dictyostelium discoideum (Slime mold)

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Q55G11 (NCSEB_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified October 19, 2011. Version 40. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Neutral ceramidase B
Short name=N-CDase B
Short name=NCDase B
EC=3.5.1.23

Alternative name(s):
Acylsphingosine deacylase 2B
N-acylsphingosine amidohydrolase 2B

Gene names

Name:	dcd2B
ORF Names:	DDB_G0268374

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	718 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid By similarity.
Catalytic activity	N-acylsphingosine + H₂O = a carboxylate + sphingosine.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the neutral ceramidase family.

Ontologies

Keywords
Biological process	Lipid metabolism Sphingolipid metabolism
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ceramide metabolic process Inferred by curator. Source: dictyBase
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ceramidase activity Inferred from sequence or structural similarity. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

20

Potential

Chain

698

Neutral ceramidase B

PRO_0000247109

Sites

Active site

1

Nucleophile By similarity

Amino acid modifications

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q55G11 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: BDAE0B6C79271AFA
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718

78,609

        10         20         30         40         50         60 
MINSFKKLII LISLVIILLS SNNIFIDSFK IPVNQKNVKS SGDSSYQIGA GIYDITGASA 

        70         80         90        100        110        120 
EVNLMGYANP LQVGAGIHFR QRARAFVFVD SNGNRAVYVS TDSCMIFQEV KIHVVELLQD 

       130        140        150        160        170        180 
IFGPNVYTEA NVLLSGTHTH SGPAGFSQYA LYGITSLGFY KKNFDTICNG IVQAIVKAHK 

       190        200        210        220        230        240 
SVQPANMFTE TGELWNTNIN RSPFAYDNNP EEEKAMYDSN VDKNMTVLRI EDMNGNPFAA 

       250        260        270        280        290        300 
ISFFAVHCTS MNNTNHLISG DNKGYASYLW EKQVNGPGTA GKGPFVAAFG QSNEGDVSPN 

       310        320        330        340        350        360 
TRGPTCRDGS PCDYKTSTCN GRNEECWSLG PGKDGDMFES TQIIGGNQFN KALELFNNAS 

       370        380        390        400        410        420 
IQVSGPVQYR HSWVQFTNVS VEPPYNSGVD NATTCRGAMG YSFAAGTTDG PGAFNFVQSD 

       430        440        450        460        470        480 
NNTSGNPFWN FIGDFIAKPT PDQIRCQSPK PILLDVGMVE PIPWVPDVMP IQIVTIGQIV 

       490        500        510        520        530        540 
LVAVPGEFTT MSGRRLRNSV REIIGESIEN PIVLIAGLSN TYSGYIATFE EYQVQRYEGA 

       550        560        570        580        590        600 
STVFGPHTLG SYMQEFGKLA QSIVDGTTVP AGPTPRNLTG HTLFFLPPVI VDAAPDFDDF 

       610        620        630        640        650        660 
GEVSIDVNLN YSVNETVSCV FYGGNPRNDF MIESSFLSVD LLTGTDQWTT VLDDGDWDTK 

       670        680        690        700        710 
FKWKMHDLGF SLITIEWVIA PDTTPGTYRI THSGFAKKNP FSSNLTPYQG ISRNFVVQ

References

[1]

"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.

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Kuspa A.
Nature 435:43-57(2005) [PubMed: 15875012] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000003 Genomic DNA. Translation: EAL73640.1.
RefSeq	XP_647372.1. XM_642280.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0232168; DDB0232168; DDB_G0268374.
GeneID	8616181.
GenomeReviews	Gene locus dcd2B in contig CM000150_GR.
KEGG	ddi:DDB_G0268374.
Organism-specific databases
dictyBase	DDB_G0268374. dcd2B.
Phylogenomic databases
eggNOG	KOG2232.
GeneTree	EPrGT00050000005921.
HOGENOM	HBG315824.
OMA	SWFAVHP.
PhylomeDB	Q55G11.
ProtClustDB	PTZ00487.
Family and domain databases
InterPro	IPR006823. Ceramidase_alk. [Graphical view]
PANTHER	PTHR12670. Ceramidase_alk. 1 hit.
Pfam	PF04734. Ceramidase_alk. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NCSEB_DICDI

Accession

Primary (citable) accession number: Q55G11

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	May 24, 2005
Last modified:	October 19, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Relevant documents

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase

SIMILARITY comments

Index of protein domains and families

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents