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Q55FM6 (ENOPH_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Enolase-phosphatase E1

EC=3.1.3.77
Alternative name(s):
2,3-diketo-5-methylthio-1-phosphopentane phosphatase
Gene names
Name:enoph1
ORF Names:DDB_G0268036
OrganismDictyostelium discoideum (Slime mold)
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length267 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) By similarity.

Catalytic activity

5-(methylthio)-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6.

Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6.

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Sequence similarities

Belongs to the HAD-like hydrolase superfamily. MasA/mtnC family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Methionine biosynthesis
   Cellular componentCytoplasm
Nucleus
   LigandMagnesium
Metal-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processL-methionine salvage from methylthioadenosine

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionacireductone synthase activity

Inferred from sequence or structural similarity. Source: UniProtKB

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 267267Enolase-phosphatase E1
PRO_0000327574

Regions

Region155 – 1562Substrate binding By similarity

Sites

Metal binding111Magnesium By similarity
Metal binding131Magnesium; via carbonyl oxygen By similarity
Metal binding2151Magnesium By similarity
Binding site1891Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q55FM6 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: 17DF886B003933B9

FASTA26730,594
        10         20         30         40         50         60 
MTPNIHTVIL DIEGTTTPIS FVHDVLFPYI RDNLVRHINQ KWGSEELKQD IKELYKLYLE 

        70         80         90        100        110        120 
DNKASELVVN NQFNTPEILN PDDESTDKEK LIESVIRNVI YQMDNDRKST PLKQLQGHMW 

       130        140        150        160        170        180 
LEGYENELVK GVVFPEVPKA FENWNLNHID IYIYSSGSIA AQKLLFNYSN FGSLLPYIKG 

       190        200        210        220        230        240 
HFDTTIGGKL HPSSYEKILS TINNGSPNSY LFVTDSILEA KAARESGLNV CLSIRDGNPP 

       250        260 
IVDRELLNTF DQVSSFDQLF NKFNFKN 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000003 Genomic DNA. Translation: EAL73471.1.
RefSeqXP_647507.1. XM_642415.1.

3D structure databases

HSSPHSSP built from PDB template 1ZS9 based on UniProtKB Q9UHY7.
ProteinModelPortalQ55FM6.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ55FM6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0230999; DDB0230999; DDB_G0268036.
GeneID8616314.
GenomeReviewsGene locus enoph1 in contig CM000150_GR.
KEGGddi:DDB_G0268036.

Organism-specific databases

dictyBaseDDB_G0268036.

Phylogenomic databases

eggNOGKOG2630.
GeneTreeEPrGT00050000005300.
HOGENOMHBG294242.
OMATTDLNFI.
PhylomeDBQ55FM6.
ProtClustDBCLSZ2431550.

Family and domain databases

InterProIPR010041. Enolase_ppase.
IPR023214. HAD-like_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.1000. HAD-like_dom. 1 hit.
SUPFAMSSF56784. HAD-like_dom. 1 hit.
TIGRFAMsTIGR01691. Enolase-ppase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameENOPH_DICDI
AccessionPrimary (citable) accession number: Q55FM6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: September 21, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families