Q55FF3 (GSTA1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative glutathione S-transferase alpha-1
EC=2.5.1.18

Alternative name(s):
GST class-alpha 1

Gene names

Name:	gsta1
ORF Names:	DDB_G0268138

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles By similarity.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Sequence similarities	Belongs to the GST superfamily. Alpha family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Molecular function	Transferase
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	phagocytic vesicle Inferred from direct assay Ref.3. Source: dictyBase
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 202	201	Putative glutathione S-transferase alpha-1		PRO_0000350738
Regions
	Domain	4 – 81	78	GST N-terminal
	Domain	83 – 200	118	GST C-terminal
	Region	51 – 52	2	Glutathione binding By similarity
	Region	65 – 66	2	Glutathione binding By similarity
Sites
	Binding site	10	1	Glutathione By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylthreonine Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q55FF3 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: ACA3DDF0E4738949	FASTA	202	23,063
	10 20 30 40 50 60 MTTNKPSFSY FKVLVLGQLP RFTLNYFGTD FTDNYVESID DEFRKTLKFG QLPLFVDSDG 70 80 90 100 110 120 FRLTQTDAIC KYIAAKHDFL GKSIKERAIV DETIAAVHDD VIIPGYKVGK GVEDKKVIDT 130 140 150 160 170 180 IISRHFTTFE NVLAENKFIA GGDNYTLADL YVFVAYHYYK HLGHSEKFQN KFPHLEALQA 190 200 HFESNKGIAE YIKNRPDSGR GI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	Bienvenut W.V., Patel H., Brunton V.G., Frame M.C. Submitted (APR-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-21; 49-71; 116-124 AND 172-186, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT THR-2, MASS SPECTROMETRY.
[3]	"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake." Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T. Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: AX2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000003 Genomic DNA. Translation: EAL73522.1.
RefSeq	XP_647580.1. XM_642488.1.
3D structure databases
HSSP	HSSP built from PDB template 1K3O based on UniProtKB P08263.
ProteinModelPortal	Q55FF3.
ModBase	Search...
Proteomic databases
PRIDE	Q55FF3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231431; DDB0231431; DDB_G0268138.
GeneID	8616392.
KEGG	ddi:DDB_G0268138.
Organism-specific databases
dictyBase	DDB_G0268138.
Phylogenomic databases
eggNOG	NOG307242.
OMA	PAFENIL.
ProtClustDB	CLSZ2430358.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTA1_DICDI

Accession

Primary (citable) accession number: Q55FF3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 23, 2008
Last sequence update:	May 24, 2005
Last modified:	May 1, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)