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Q55FF3 (GSTA1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative glutathione S-transferase alpha-1

EC=2.5.1.18
Alternative name(s):
GST class-alpha 1
Gene names
Name:gsta1
ORF Names:DDB_G0268138
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length202 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles By similarity.

Catalytic activity

RX + glutathione = HX + R-S-glutathione.

Sequence similarities

Belongs to the GST superfamily. Alpha family.

Contains 1 GST C-terminal domain.

Contains 1 GST N-terminal domain.

Ontologies

Keywords
   Molecular functionTransferase
   PTMAcetylation
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Cellular_componentphagocytic vesicle

Inferred from direct assay Ref.3. Source: dictyBase

   Molecular_functionglutathione transferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.2
Chain2 – 202201Putative glutathione S-transferase alpha-1
PRO_0000350738

Regions

Domain4 – 8178GST N-terminal
Domain83 – 200118GST C-terminal
Region51 – 522Glutathione binding By similarity
Region65 – 662Glutathione binding By similarity

Sites

Binding site101Glutathione By similarity

Amino acid modifications

Modified residue21N-acetylthreonine Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q55FF3 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: ACA3DDF0E4738949

FASTA20223,063
        10         20         30         40         50         60 
MTTNKPSFSY FKVLVLGQLP RFTLNYFGTD FTDNYVESID DEFRKTLKFG QLPLFVDSDG 

        70         80         90        100        110        120 
FRLTQTDAIC KYIAAKHDFL GKSIKERAIV DETIAAVHDD VIIPGYKVGK GVEDKKVIDT 

       130        140        150        160        170        180 
IISRHFTTFE NVLAENKFIA GGDNYTLADL YVFVAYHYYK HLGHSEKFQN KFPHLEALQA 

       190        200 
HFESNKGIAE YIKNRPDSGR GI 

« Hide

References

« Hide 'large scale' references
[1]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]Bienvenut W.V., Patel H., Brunton V.G., Frame M.C.
Submitted (APR-2008) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 2-21; 49-71; 116-124 AND 172-186, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT THR-2, IDENTIFICATION BY MASS SPECTROMETRY.
[3]"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake."
Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T.
Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: AX2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000003 Genomic DNA. Translation: EAL73522.1.
RefSeqXP_647580.1. XM_642488.1.

3D structure databases

ProteinModelPortalQ55FF3.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ55FF3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0231431; DDB0231431; DDB_G0268138.
GeneID8616392.
KEGGddi:DDB_G0268138.

Organism-specific databases

dictyBaseDDB_G0268138.

Phylogenomic databases

eggNOGNOG307242.
OMACETREDL.
PhylomeDBQ55FF3.

Family and domain databases

Gene3D1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSTA1_DICDI
AccessionPrimary (citable) accession number: Q55FF3
Entry history
Integrated into UniProtKB/Swiss-Prot: September 23, 2008
Last sequence update: May 24, 2005
Last modified: July 9, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase