Reviewed, UniProtKB/Swiss-Prot Q55F94 (DLPA_DICDI)

Names and origin

Protein names

Recommended name:
    Dynamin-like protein A
    EC=3.6.5.5

Gene names

Name:	dlpA
Synonyms:	slb233
ORF Names:	DDB_G0268592

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	880 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in cytokinesis. May hydrolyze GTP. Ref.4
Catalytic activity	GTP + H2O = GDP + phosphate.
Subcellular location	Cytoplasm Potential. Note: During cytokinesis, found in the cleavage furrow. Ref.4
Developmental stage	Expression begins during cell cycle progression, between 12 and 24 hours after germination. Reach a maximum around mid-log phase and disappear during the stationary phase. Expressed in the central funnel of cells of migrating slugs and at the top of rising culminants. Not expressed until the slug stage. Ref.4 Ref.2
Induction	Induced by STATa. Ref.3
Disruption phenotype	Produced cells are larger and a large amount of them contains more than 2 nuclei. Ref.4
Sequence similarities	Belongs to the dynamin family.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Ligand	GTP-binding Nucleotide-binding
Molecular function	Hydrolase Motor protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro motor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q55F94-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q55F94-2)

The sequence of this isoform differs from the canonical sequence as follows:
     19-19: E → EVDNKKI

Isoform 3 (identifier: Q55F94-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: MSVFKKKDKSDDKKKKHDE → MISCTNHHRTIYQNNLNNSINNSNNTVDNKKI

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 880	880	Dynamin-like protein A		PRO_0000371337
Regions
	Nucleotide binding	201 – 208	8	GTP Potential
	Nucleotide binding	315 – 319	5	GTP Potential
	Nucleotide binding	380 – 383	4	GTP Potential
	Coiled coil	69 – 152	84	Potential
	Coiled coil	479 – 509	31	Potential
	Coiled coil	824 – 861	38	Potential
	Compositional bias	13 – 16	4	Poly-Lys
	Compositional bias	96 – 99	4	Poly-Ala
	Compositional bias	132 – 138	7	Poly-Ala
Natural variations
	Alternative sequence	1 – 19	19	MSVFK…KKHDE → MISCTNHHRTIYQNNLNNSI NNSNNTVDNKKI in isoform 3.		VSP_037019
	Alternative sequence	19	1	E → EVDNKKI in isoform 2.		VSP_037020

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 055ACA2D0D209512	FASTA	880	100,009
	10 20 30 40 50 60 MSVFKKKDKS DDKKKKHDEE TPQGTFQPAS QSTSNTNLNS LASSVNNGAS VGSTNGSTPN 70 80 90 100 110 120 NSNGSTPTYN HNNSAEELEK QKKEEDEKRK KSELEAAAAV VAKKKEDEEK QRKEQVELER 130 140 150 160 170 180 KRRDEEIRRT NAAAANAANK ELNEQVEISS LEQMGKLDPS STIHDMFSFF PNEVYNSYEK 190 200 210 220 230 240 LQYFSRDLNT AVSHPEIVFV GPRSSGKSSL IEAFIGRALN IVGGGNIVGV GGSNANGCSK 250 260 270 280 290 300 RVLYLQFTNN IDFEVPKVTI KKDNTIKEFD HDIIVSIEQL NENLAKRNQL TNDYIEEPIY 310 320 330 340 350 360 VSIESRTTLN LTLIDSPGLL FDQSQAESNK IESIVSSLLR PSHRLIIAVE SCSQDWKSMS 370 380 390 400 410 420 MGQYLKKIDP ELSRSTFVFT KFHHTVRGFS STRDINKYLS GTVPDIKGFF VTLPNHQVRA 430 440 450 460 470 480 SYSEANRFQE KIYQAHKRDM HALEQLQYDK RYERTIGVAP LRRYILNIVW KSYQDTIPRI 490 500 510 520 530 540 LKHLRSKRQT AEATLNELQK QSSSLDSTKL RSIASNYTVT FLQITEKLLS GTSEGNPSAN 550 560 570 580 590 600 GQTLDEEKSQ QGDCGEWVDA YKEAIYIDPE EWNIPYWSSK LYGGQQLERL MAEFKAVCDN 610 620 630 640 650 660 SKISEVKMDD IATASGINKL NNIPNYAWAA SDLTSLISRD TFVPLIEQLC ERAMYIMKRL 670 680 690 700 710 720 TDIADKVIDS RRKSRCIGSS PFGGNGLNNI NSGGNNGINI NDRLSNTDMD SLDQYPFFTH 730 740 750 760 770 780 HVKNLYYDFV HRAAKGCKEK CMDEFYSSRT IYWELTEHPD SSLPSIRNDH HETKTAVCQL 790 800 810 820 830 840 ATKLFDSIRQ RITKNVLLKL YNFFLVPMQT DLWNEIQAQI TCLSNEQLEQ LFEVQATREQ 850 860 870 880 LKQEEKKQQQ ILEKYSQIDE QFLKAASLFC RPLSNPTPSA
	Isoform 2. Checksum: 95105711D627AFCA	FASTA	886	100,707
	10 20 30 40 50 60 MSVFKKKDKS DDKKKKHDEV DNKKIETPQG TFQPASQSTS NTNLNSLASS VNNGASVGST 70 80 90 100 110 120 NGSTPNNSNG STPTYNHNNS AEELEKQKKE EDEKRKKSEL EAAAAVVAKK KEDEEKQRKE 130 140 150 160 170 180 QVELERKRRD EEIRRTNAAA ANAANKELNE QVEISSLEQM GKLDPSSTIH DMFSFFPNEV 190 200 210 220 230 240 YNSYEKLQYF SRDLNTAVSH PEIVFVGPRS SGKSSLIEAF IGRALNIVGG GNIVGVGGSN 250 260 270 280 290 300 ANGCSKRVLY LQFTNNIDFE VPKVTIKKDN TIKEFDHDII VSIEQLNENL AKRNQLTNDY 310 320 330 340 350 360 IEEPIYVSIE SRTTLNLTLI DSPGLLFDQS QAESNKIESI VSSLLRPSHR LIIAVESCSQ 370 380 390 400 410 420 DWKSMSMGQY LKKIDPELSR STFVFTKFHH TVRGFSSTRD INKYLSGTVP DIKGFFVTLP 430 440 450 460 470 480 NHQVRASYSE ANRFQEKIYQ AHKRDMHALE QLQYDKRYER TIGVAPLRRY ILNIVWKSYQ 490 500 510 520 530 540 DTIPRILKHL RSKRQTAEAT LNELQKQSSS LDSTKLRSIA SNYTVTFLQI TEKLLSGTSE 550 560 570 580 590 600 GNPSANGQTL DEEKSQQGDC GEWVDAYKEA IYIDPEEWNI PYWSSKLYGG QQLERLMAEF 610 620 630 640 650 660 KAVCDNSKIS EVKMDDIATA SGINKLNNIP NYAWAASDLT SLISRDTFVP LIEQLCERAM 670 680 690 700 710 720 YIMKRLTDIA DKVIDSRRKS RCIGSSPFGG NGLNNINSGG NNGININDRL SNTDMDSLDQ 730 740 750 760 770 780 YPFFTHHVKN LYYDFVHRAA KGCKEKCMDE FYSSRTIYWE LTEHPDSSLP SIRNDHHETK 790 800 810 820 830 840 TAVCQLATKL FDSIRQRITK NVLLKLYNFF LVPMQTDLWN EIQAQITCLS NEQLEQLFEV 850 860 870 880 QATREQLKQE EKKQQQILEK YSQIDEQFLK AASLFCRPLS NPTPSA
	Isoform 3. Checksum: 1682C83CBFDA262A	FASTA	893	101,404
	10 20 30 40 50 60 MISCTNHHRT IYQNNLNNSI NNSNNTVDNK KIETPQGTFQ PASQSTSNTN LNSLASSVNN 70 80 90 100 110 120 GASVGSTNGS TPNNSNGSTP TYNHNNSAEE LEKQKKEEDE KRKKSELEAA AAVVAKKKED 130 140 150 160 170 180 EEKQRKEQVE LERKRRDEEI RRTNAAAANA ANKELNEQVE ISSLEQMGKL DPSSTIHDMF 190 200 210 220 230 240 SFFPNEVYNS YEKLQYFSRD LNTAVSHPEI VFVGPRSSGK SSLIEAFIGR ALNIVGGGNI 250 260 270 280 290 300 VGVGGSNANG CSKRVLYLQF TNNIDFEVPK VTIKKDNTIK EFDHDIIVSI EQLNENLAKR 310 320 330 340 350 360 NQLTNDYIEE PIYVSIESRT TLNLTLIDSP GLLFDQSQAE SNKIESIVSS LLRPSHRLII 370 380 390 400 410 420 AVESCSQDWK SMSMGQYLKK IDPELSRSTF VFTKFHHTVR GFSSTRDINK YLSGTVPDIK 430 440 450 460 470 480 GFFVTLPNHQ VRASYSEANR FQEKIYQAHK RDMHALEQLQ YDKRYERTIG VAPLRRYILN 490 500 510 520 530 540 IVWKSYQDTI PRILKHLRSK RQTAEATLNE LQKQSSSLDS TKLRSIASNY TVTFLQITEK 550 560 570 580 590 600 LLSGTSEGNP SANGQTLDEE KSQQGDCGEW VDAYKEAIYI DPEEWNIPYW SSKLYGGQQL 610 620 630 640 650 660 ERLMAEFKAV CDNSKISEVK MDDIATASGI NKLNNIPNYA WAASDLTSLI SRDTFVPLIE 670 680 690 700 710 720 QLCERAMYIM KRLTDIADKV IDSRRKSRCI GSSPFGGNGL NNINSGGNNG ININDRLSNT 730 740 750 760 770 780 DMDSLDQYPF FTHHVKNLYY DFVHRAAKGC KEKCMDEFYS SRTIYWELTE HPDSSLPSIR 790 800 810 820 830 840 NDHHETKTAV CQLATKLFDS IRQRITKNVL LKLYNFFLVP MQTDLWNEIQ AQITCLSNEQ 850 860 870 880 890 LEQLFEVQAT REQLKQEEKK QQQILEKYSQ IDEQFLKAAS LFCRPLSNPT PSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORMS 1; 2 AND 3). Strain: AX4.
[2]	"Changing patterns of gene expression in Dictyostelium prestalk cell subtypes recognized by in situ hybridization with genes from microarray analyses." Maeda M., Sakamoto H., Iranfar N., Fuller D., Maruo T., Ogihara S., Morio T., Urushihara H., Tanaka Y., Loomis W.F. Eukaryot. Cell 2:627-637(2003) [PubMed: 12796308] [Abstract] Cited for: DEVELOPMENTAL STAGE.
[3]	"Identification of new modes of Dd-STATa regulation of gene expression in Dictyostelium by in situ hybridisation." Shimada N., Maeda M., Urushihara H., Kawata T. Int. J. Dev. Biol. 48:679-682(2004) [PubMed: 15470642] [Abstract] Cited for: INDUCTION.
[4]	"Evolutionary linkage between eukaryotic cytokinesis and chloroplast division by dynamin proteins." Miyagishima S.Y., Kuwayama H., Urushihara H., Nakanishi H. Proc. Natl. Acad. Sci. U.S.A. 105:15202-15207(2008) [PubMed: 18809930] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000003 Genomic DNA. Translation: EDR41124.1. AAFI02000003 Genomic DNA. Translation: EAL73749.1.
RefSeq	XP_001732949.1. XP_647639.1.
3D structure databases
SMR	Q55F94. Positions 178-487.
ModBase	Search...
Genome annotation databases
GeneID	8616454.
KEGG	ddi:DDB_0229901.
Organism-specific databases
dictyBase	DDB_G0268592. dlpA.
Phylogenomic databases
eggNOG	KOG0446.
HOGENOM	HBG464489.
Family and domain databases
InterPro	IPR001401. Dynamin_GTPase. [Graphical view]
Pfam	PF00350. Dynamin_N. 1 hit. [Graphical view]
PRINTS	PR00195. DYNAMIN.
ProtoNet	Search...

Entry information

Entry name

DLPA_DICDI

Accession

Primary (citable) accession number: Q55F94
Secondary accession number(s): B0G0Y6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	May 24, 2005
Last modified:	February 9, 2010
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)