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Protein

Enolase B

Gene

enoB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+By similarityNote: Mg2+ is required for catalysis and for stabilizing the dimer.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gpdA)
  2. Phosphoglycerate kinase (pgkA)
  3. no protein annotated in this organism
  4. Enolase A (enoA), Enolase B (enoB)
  5. Pyruvate kinase (pyk)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei156SubstrateBy similarity1
Binding sitei165SubstrateBy similarity1
Active sitei208Proton donorBy similarity1
Metal bindingi243MagnesiumBy similarity1
Metal bindingi296MagnesiumBy similarity1
Binding sitei296SubstrateBy similarity1
Metal bindingi323MagnesiumBy similarity1
Binding sitei323SubstrateBy similarity1
Active sitei348Proton acceptorBy similarity1
Binding sitei399SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase B (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase B
2-phosphoglycerate dehydratase B
Gene namesi
Name:enoB
ORF Names:DDB_G0268214
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 1, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0268214. enoB.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003303511 – 443Enolase BAdd BLAST443

Proteomic databases

PaxDbiQ55F83.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi44689.DDB0231356.

Structurei

3D structure databases

ProteinModelPortaliQ55F83.
SMRiQ55F83.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni375 – 378Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

eggNOGiKOG2670. Eukaryota.
COG0148. LUCA.
InParanoidiQ55F83.
KOiK01689.
OMAiDWPAWSQ.
PhylomeDBiQ55F83.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR034390. Enolase-like_superfamily.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SFLDS00001. Enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q55F83-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIESIKARE ILDSRGNPTL EVEVWTSEGM FSAKVPSGAS TGIHEAFELR
60 70 80 90 100
DGDKSRYFGY GVLNAIVNVN NLISPTLKGM RVDNQKAIDD KMIELDGTKN
110 120 130 140 150
KSKLGSNAIV GVSMAVARAA ASIKGVPLYR YISQLAGNSN IRLPVPCFNV
160 170 180 190 200
INGGKHAGNR LPFQEFMLVP IGAPKFKEAL RYGSEVYHSL KEIIKERYGL
210 220 230 240 250
DATNVGDEGG FAPNLTSPEE ALTLLVDAIN HSGYQDVIKI GIDSAASEFW
260 270 280 290 300
NPKTSKYDMD FKNIGSSHLK SSLNSGEQLM SRYLEILKKY PIAFFEDPFG
310 320 330 340 350
EDDWENHGKI TAAIGNKIQI IGDDLLCTNP ERIKKAISEK TVNSLLLKIN
360 370 380 390 400
QIGTLTETIE AAKLAKEAGW GCLVSHRSGE TDDHFIADLV VGLGTGEIKS
410 420 430 440
GAPCRFERLS KYNRLMKIEE ELEKLHRSNG NSFEYAGEEF YHF
Length:443
Mass (Da):48,820
Last modified:May 24, 2005 - v1
Checksum:i5ADDB5C1D18AC051
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000003 Genomic DNA. Translation: EAL73560.1.
RefSeqiXP_647650.1. XM_642558.1.

Genome annotation databases

EnsemblProtistsiEAL73560; EAL73560; DDB_G0268214.
GeneIDi8616467.
KEGGiddi:DDB_G0268214.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiENOB_DICDI
AccessioniPrimary (citable) accession number: Q55F83
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 24, 2005
Last modified: June 7, 2017
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families