Q55EE0 (DHAK_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable dihydroxyacetone kinase
Short name=DHA kinase
EC=2.7.1.29

Alternative name(s):
Glycerone kinase

Gene names

Name:	dhak
Synonyms:	dak
ORF Names:	DDB_G0269274

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	648 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of dihydroxyacetone By similarity.
Catalytic activity	ATP + glycerone = ADP + glycerone phosphate.
Sequence similarities	Belongs to the dihydroxyacetone kinase (DAK) family. Contains 1 DhaK domain. Contains 1 DhaL domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Lyase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycerol metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerone kinase activity Inferred from electronic annotation. Source: EC lyase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 648	648	Probable dihydroxyacetone kinase		PRO_0000328437
Regions
	Domain	7 – 391	385	DhaK
	Domain	433 – 645	213	DhaL
	Nucleotide binding	462 – 465	4	ATP By similarity
	Nucleotide binding	506 – 507	2	ATP By similarity
	Nucleotide binding	562 – 563	2	ATP By similarity
	Nucleotide binding	630 – 632	3	ATP By similarity
	Region	55 – 58	4	Substrate binding By similarity
	Compositional bias	279 – 301	23	Poly-Asn
Sites
	Active site	247	1	Tele-hemiaminal-histidine intermediate By similarity
	Binding site	106	1	Substrate By similarity
	Binding site	111	1	Substrate By similarity
	Binding site	554	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q55EE0 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: F841383A0B1EEE92	FASTA	648	71,006
	10 20 30 40 50 60 MKKIINNPQN VVSEMIDGFT QSSRDLLKLK GNFNVVVRSD YSQIKDRVTL ISGGGSGHEP 70 80 90 100 110 120 AHIGYIGNAM LTGAVCGDVF ASPSAKQIFM AIKSVAGKMG CILIVKNYMG DNGSFSIARE 130 140 150 160 170 180 MCKSQLPDIR VEIITVDDDI SSILMKLNEF SNDNNDNIQD IRDKYKSITN RRGIAGTVLV 190 200 210 220 230 240 HKILGGLAEQ GKSIDEILKF YNKYISPSKS LNLVTMGVGL SSCIIPSVGS PSFTLNEKEM 250 260 270 280 290 300 EIGLGIHGEF GIEKVELKPS KQIIKSLIDN LLKILPYSNN NNNNNNNNNN NNNNNNNNNN 310 320 330 340 350 360 NSCGISCGSD GEDKSLIVLI NNLGSTTNME MAIATNDCLN YLHEKGFTVE RLITGTLMTS 370 380 390 400 410 420 LEMAGISISL LLIKNNQIIN LIDLKTNAMG WPNSVLKPYK NKEDSILTLD ENDSHEIKYD 430 440 450 460 470 480 NLKSITISKE NGEILKEIVL LGCNSLIENS NKLTDLDKQV GDGDLGTTLE NLSKSIKKSI 490 500 510 520 530 540 DTIPFDKPCY TFRKISLIIQ ELIGGSSGLF YSIFFLRLSN SLYERSTVNG KNQISTNDWG 550 560 570 580 590 600 LSLIDAVNAI KELGKADIGD CTMLDSLIPA INKINQCCKD NENSSSSFDL LNTLKLASKE 610 620 630 640 AQLGSESTIE MIAKKGRSSY LGERTSHIMD PGAHAIEIIF KSFLSIKK

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000005 Genomic DNA. Translation: EAL71987.1.
RefSeq	XP_645841.1. XM_640749.1.
3D structure databases
HSSP	HSSP built from PDB template 2IU6 based on UniProtKB Q9CIW0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q55EE0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0266459; DDB0266459; DDB_G0269274.
GeneID	8616785.
GenomeReviews	Gene locus dhak in contig CM000150_GR.
KEGG	ddi:DDB_G0269274.
Organism-specific databases
dictyBase	DDB_G0269274. dhak.
Phylogenomic databases
eggNOG	KOG2426.
GeneTree	EPrGT00050000002529.
HOGENOM	HBG473357.
OMA	SHTASIG.
PhylomeDB	Q55EE0.
ProtClustDB	PTZ00375.
Family and domain databases
InterPro	IPR004006. Dak1. IPR004007. Dak2. [Graphical view]
Pfam	PF02733. Dak1. 2 hits. PF02734. Dak2. 1 hit. [Graphical view]
SUPFAM	SSF101473. Dak2. 1 hit.
PROSITE	PS51481. DHAK. 1 hit. PS51480. DHAL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHAK_DICDI

Accession

Primary (citable) accession number: Q55EE0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	September 21, 2011
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)