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Q55E72 (PKS1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable polyketide synthase 1
Short name=dipks1

Including the following 2 domains:

  1. Polyketide synthase stlA
    EC=2.3.1.-
  2. Chalcone synthase stlA
    EC=2.3.1.74
    Alternative name(s):
    Steely1
Gene names
Name:stlA
Synonyms:pks1
ORF Names:DDB_G0269364
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length3147 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable polyketide synthase By similarity. Produces only acylpyrones; in vitro. Ref.3

Catalytic activity

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.

Cofactor

Binds 1 phosphopantetheine covalently By similarity.

Pathway

Secondary metabolite biosynthesis; flavonoid biosynthesis.

Subunit structure

Homodimer. Ref.3

Developmental stage

Expressed during development. Expressed maximally in early development before cellular aggregation. Ref.3

Domain

Modular protein possessing six classical catalytic domains and a type III polyketide synthase domain. May facilitate covalent transfer of steely N-terminal acyl products directly to the C-terminal type III PKS active sites, which catalyze both iterative polyketide extension and cyclization.

Miscellaneous

In reference to their hybrid nature and to their discovery in D.discoideum, authors (Ref.3) term these type I FAS-type III PKS fusion enzymes 'steely'.

Encoded by one of the numerous copies of polyketide synthase genes localized in chromosome 1.

Sequence similarities

In the C-terminal section; belongs to the chalcone/stilbene synthases family.

Contains 1 acyl carrier domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 31473147Probable polyketide synthase 1
PRO_0000377901

Regions

Domain2573 – 264270Acyl carrier
Region161 – 21454Beta-ketoacyl synthase
Region672 – 70534Acyl/malonyl transferase
Region2789 – 3147359Chalcone synthase
Compositional bias355 – 3628Poly-Asp
Compositional bias470 – 4789Poly-Asn
Compositional bias561 – 5644Poly-Ser
Compositional bias576 – 5816Poly-Thr
Compositional bias1684 – 16885Poly-Ser
Compositional bias2097 – 21004Poly-Gln
Compositional bias2647 – 26559Poly-Asn
Compositional bias2770 – 27745Poly-Gln

Sites

Active site1801For beta-ketoacyl synthase activity By similarity
Active site6821For acyl/malonyl transferase activity By similarity
Active site29301 By similarity

Amino acid modifications

Modified residue26051O-(pantetheine 4'-phosphoryl)serine By similarity

Secondary structure

1 ......................................................... 3147
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q55E72 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: 6B67391AB6596F46

FASTA3,147352,195
        10         20         30         40         50         60 
MNKNSKIQSP NSSDVAVIGV GFRFPGNSND PESLWNNLLD GFDAITQVPK ERWATSFREM 

        70         80         90        100        110        120 
GLIKNKFGGF LKDSEWKNFD PLFFGIGPKE APFIDPQQRL LLSIVWESLE DAYIRPDELR 

       130        140        150        160        170        180 
GSNTGVFIGV SNNDYTKLGF QDNYSISPYT MTGSNSSLNS NRISYCFDFR GPSITVDTAC 

       190        200        210        220        230        240 
SSSLVSVNLG VQSIQMGECK IAICGGVNAL FDPSTSVAFS KLGVLSENGR CNSFSDQASG 

       250        260        270        280        290        300 
YVRSEGAGVV VLKSLEQAKL DGDRIYGVIK GVSSNEDGAS NGDKNSLTTP SCEAQSINIS 

       310        320        330        340        350        360 
KAMEKASLSP SDIYYIEAHG TGTPVGDPIE VKALSKIFSN SNNNQLNNFS TDGNDNDDDD 

       370        380        390        400        410        420 
DDNTSPEPLL IGSFKSNIGH LESAAGIASL IKCCLMLKNR MLVPSINCSN LNPSIPFDQY 

       430        440        450        460        470        480 
NISVIREIRQ FPTDKLVNIG INSFGFGGSN CHLIIQEYNN NFKNNSTICN NNNNNNNNID 

       490        500        510        520        530        540 
YLIPISSKTK KSLDKYLILI KTNSNYHKDI SFDDFVKFQI KSKQYNLSNR MTTIANDWNS 

       550        560        570        580        590        600 
FIKGSNEFHN LIESKDGEGG SSSSNRGIDS ANQINTTTTS TINDIEPLLV FVFCGQGPQW 

       610        620        630        640        650        660 
NGMIKTLYNS ENVFKNTVDH VDSILYKYFG YSILNVLSKI DDNDDSINHP IVAQPSLFLL 

       670        680        690        700        710        720 
QIGLVELFKY WGIYPSISVG HSFGEVSSYY LSGIISLETA CKIVYVRSSN QNKTMGSGKM 

       730        740        750        760        770        780 
LVVSMGFKQW NDQFSAEWSD IEIACYNAPD SIVVTGNEER LKELSIKLSD ESNQIFNTFL 

       790        800        810        820        830        840 
RSPCSFHSSH QEVIKGSMFE ELSNLQSTGE TEIPLFSTVT GRQVLSGHVT AQHIYDNVRE 

       850        860        870        880        890        900 
PVLFQKTIES ITSYIKSHYP SNQKVIYVEI APHPTLFSLI KKSIPSSNKN SSSVLCPLNR 

       910        920        930        940        950        960 
KENSNNSYKK FVSQLYFNGV NVDFNFQLNS ICDNVNNDHH LNNVKQNSFK ETTNSLPRYQ 

       970        980        990       1000       1010       1020 
WEQDEYWSEP LISRKNRLEG PTTSLLGHRI IYSFPVFQSV LDLQSDNYKY LLDHLVNGKP 

      1030       1040       1050       1060       1070       1080 
VFPGAGYLDI IIEFFDYQKQ QLNSSDSSNS YIINVDKIQF LNPIHLTENK LQTLQSSFEP 

      1090       1100       1110       1120       1130       1140 
IVTKKSAFSV NFFIKDTVED QSKVKSMSDE TWTNTCKATI SLEQQQPSPS STLTLSKKQD 

      1150       1160       1170       1180       1190       1200 
LQILRNRCDI SKLDKFELYD KISKNLGLQY NSLFQVVDTI ETGKDCSFAT LSLPEDTLFT 

      1210       1220       1230       1240       1250       1260 
TILNPCLLDN CFHGLLTLIN EKGSFVVESI SSVSIYLENI GSFNQTSVGN VQFYLYTTIS 

      1270       1280       1290       1300       1310       1320 
KATSFSSEGT CKLFTKDGSL ILSIGKFIIK STNPKSTKTN ETIESPLDET FSIEWQSKDS 

      1330       1340       1350       1360       1370       1380 
PIPTPQQIQQ QSPLNSNPSF IRSTILKDIQ FEQYCSSIIH KELINHEKYK NQQSFDINSL 

      1390       1400       1410       1420       1430       1440 
ENHLNDDQLM ESLSISKEYL RFFTRIISII KQYPKILNEK ELKELKEIIE LKYPSEVQLL 

      1450       1460       1470       1480       1490       1500 
EFEVIEKVSM IIPKLLFEND KQSSMTLFQD NLLTRFYSNS NSTRFYLERV SEMVLESIRP 

      1510       1520       1530       1540       1550       1560 
IVREKRVFRI LEIGAGTGSL SNVVLTKLNT YLSTLNSNGG SGYNIIIEYT FTDISANFII 

      1570       1580       1590       1600       1610       1620 
GEIQETMCNL YPNVTFKFSV LDLEKEIINS SDFLMGDYDI VLMAYVIHAV SNIKFSIEQL 

      1630       1640       1650       1660       1670       1680 
YKLLSPRGWL LCIEPKSNVV FSDLVFGCFN QWWNYYDDIR TTHCSLSESQ WNQLLLNQSL 

      1690       1700       1710       1720       1730       1740 
NNESSSSSNC YGGFSNVSFI GGEKDVDSHS FILHCQKESI SQMKLATTIN NGLSSGSIVI 

      1750       1760       1770       1780       1790       1800 
VLNSQQLTNM KSYPKVIEYI QEATSLCKTI EIIDSKDVLN STNSVLEKIQ KSLLVFCLLG 

      1810       1820       1830       1840       1850       1860 
YDLLENNYQE QSFEYVKLLN LISTTASSSN DKKPPKVLLI TKQSERISRS FYSRSLIGIS 

      1870       1880       1890       1900       1910       1920 
RTSMNEYPNL SITSIDLDTN DYSLQSLLKP IFSNSKFSDN EFIFKKGLMF VSRIFKNKQL 

      1930       1940       1950       1960       1970       1980 
LESSNAFETD SSNLYCKASS DLSYKYAIKQ SMLTENQIEI KVECVGINFK DNLFYKGLLP 

      1990       2000       2010       2020       2030       2040 
QEIFRMGDIY NPPYGLECSG VITRIGSNVT EYSVGQNVFG FARHSLGSHV VTNKDLVILK 

      2050       2060       2070       2080       2090       2100 
PDTISFSEAA SIPVVYCTAW YSLFNIGQLS NEESILIHSA TGGVGLASLN LLKMKNQQQQ 

      2110       2120       2130       2140       2150       2160 
PLTNVYATVG SNEKKKFLID NFNNLFKEDG ENIFSTRDKE YSNQLESKID VILNTLSGEF 

      2170       2180       2190       2200       2210       2220 
VESNFKSLRS FGRLIDLSAT HVYANQQIGL GNFKFDHLYS AVDLERLIDE KPKLLQSILQ 

      2230       2240       2250       2260       2270       2280 
RITNSIVNGS LEKIPITIFP STETKDAIEL LSKRSHIGKV VVDCTDISKC NPVGDVITNF 

      2290       2300       2310       2320       2330       2340 
SMRLPKPNYQ LNLNSTLLIT GQSGLSIPLL NWLLSKSGGN VKNVVIISKS TMKWKLQTMI 

      2350       2360       2370       2380       2390       2400 
SHFVSGFGIH FNYVQVDISN YDALSEAIKQ LPSDLPPITS VFHLAAIYND VPMDQVTMST 

      2410       2420       2430       2440       2450       2460 
VESVHNPKVL GAVNLHRISV SFGWKLNHFV LFSSITAITG YPDQSIYNSA NSILDALSNF 

      2470       2480       2490       2500       2510       2520 
RRFMGLPSFS INLGPMKDEG KVSTNKSIKK LFKSRGLPSL SLNKLFGLLE VVINNPSNHV 

      2530       2540       2550       2560       2570       2580 
IPSQLICSPI DFKTYIESFS TMRPKLLHLQ PTISKQQSSI INDSTKASSN ISLQDKITSK 

      2590       2600       2610       2620       2630       2640 
VSDLLSIPIS KINFDHPLKH YGLDSLLTVQ FKSWIDKEFE KNLFTHIQLA TISINSFLEK 

      2650       2660       2670       2680       2690       2700 
VNGLSTNNNN NNNSNVKSSP SIVKEEIVTL DKDQQPLLLK EHQHIIISPD IRINKPKRES 

      2710       2720       2730       2740       2750       2760 
LIRTPILNKF NQITESIITP STPSLSQSDV LKTPPIKSLN NTKNSSLINT PPIQSVQQHQ 

      2770       2780       2790       2800       2810       2820 
KQQQKVQVIQ QQQQPLSRLS YKSNNNSFVL GIGISVPGEP ISQQSLKDSI SNDFSDKAET 

      2830       2840       2850       2860       2870       2880 
NEKVKRIFEQ SQIKTRHLVR DYTKPENSIK FRHLETITDV NNQFKKVVPD LAQQACLRAL 

      2890       2900       2910       2920       2930       2940 
KDWGGDKGDI THIVSVTSTG IIIPDVNFKL IDLLGLNKDV ERVSLNLMGC LAGLSSLRTA 

      2950       2960       2970       2980       2990       3000 
ASLAKASPRN RILVVCTEVC SLHFSNTDGG DQMVASSIFA DGSAAYIIGC NPRIEETPLY 

      3010       3020       3030       3040       3050       3060 
EVMCSINRSF PNTENAMVWD LEKEGWNLGL DASIPIVIGS GIEAFVDTLL DKAKLQTSTA 

      3070       3080       3090       3100       3110       3120 
ISAKDCEFLI HTGGKSILMN IENSLGIDPK QTKNTWDVYH AYGNMSSASV IFVMDHARKS 

      3130       3140 
KSLPTYSISL AFGPGLAFEG CFLKNVV

« Hide

References

« Hide 'large scale' references
[1]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]"Polyketide synthase genes and the natural products potential of Dictyostelium discoideum."
Zucko J., Skunca N., Curk T., Zupan B., Long P.F., Cullum J., Kessin R.H., Hranueli D.
Bioinformatics 23:2543-2549(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
[3]"Biosynthesis of Dictyostelium discoideum differentiation-inducing factor by a hybrid type I fatty acid-type III polyketide synthase."
Austin M.B., Saito T., Bowman M.E., Haydock S., Kato A., Moore B.S., Kay R.R., Noel J.P.
Nat. Chem. Biol. 2:494-502(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 2776-3147, FUNCTION, DEVELOPMENTAL STAGE, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AAFI02000005 Genomic DNA. Translation: EAL72032.1.
RefSeqXP_645909.1. XM_640817.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2H84X-ray2.90A/B2776-3147[»]
ProteinModelPortalQ55E72.
SMRQ55E72. Positions 2785-3147.
ModBaseSearch...

Protein-protein interaction databases

STRING44689.DDBDRAFT_0190208.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0234164; DDB0234164; DDB_G0269364.
GeneID8616850.
KEGGddi:DDB_G0269364.

Organism-specific databases

dictyBaseDDB_G0269364. stlA.

Phylogenomic databases

eggNOGCOG3424.
InParanoidQ55E72.
OMANDDSINH.
ProtClustDBCLSZ2729190.

Enzyme and pathway databases

UniPathwayUPA00154.

Family and domain databases

Gene3D1.10.1200.10. 1 hit.
3.40.366.10. 2 hits.
3.40.47.10. 4 hits.
3.40.50.720. 2 hits.
InterProIPR001227. Ac_transferase_dom.
IPR009081. Acyl_carrier_prot-like.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR013154. ADH_GroES-like.
IPR012328. Chalcone/stilbene_synth_C.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011032. GroES-like.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016036. Malonyl_transacylase_ACP-bd.
IPR013217. Methyltransf_12.
IPR016040. NAD(P)-bd_dom.
IPR020801. PKS_acyl_transferase.
IPR020841. PKS_Beta-ketoAc_synthase_dom.
IPR020843. PKS_ER.
IPR013968. PKS_KR.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamPF00698. Acyl_transf_1. 1 hit.
PF08240. ADH_N. 1 hit.
PF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
PF08659. KR. 1 hit.
PF08242. Methyltransf_12. 1 hit.
PF00550. PP-binding. 1 hit.
[Graphical view]
SMARTSM00827. PKS_AT. 1 hit.
SM00829. PKS_ER. 1 hit.
SM00825. PKS_KS. 1 hit.
[Graphical view]
SUPFAMSSF47336. ACP_like. 1 hit.
SSF52151. Acyl_Trfase/lysoPlipase. 1 hit.
SSF50129. GroES_like. 1 hit.
SSF55048. Malonyl_transacylase_ACP-bd. 1 hit.
SSF53901. Thiolase-like. 3 hits.
PROSITEPS50075. ACP_DOMAIN. 1 hit.
PS00606. B_KETOACYL_SYNTHASE. 1 hit.
PS00441. CHALCONE_SYNTH. False negative.
PS00012. PHOSPHOPANTETHEINE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ55E72.

Entry information

Entry namePKS1_DICDI
AccessionPrimary (citable) accession number: Q55E72
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: May 24, 2005
Last modified: May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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