Reviewed, UniProtKB/Swiss-Prot Q55E44 (DHKE_DICDI)

Names and origin

Protein names

Recommended name:
    Hybrid signal transduction histidine kinase E
    EC=2.7.13.3

Gene names

Name:	dhkE
ORF Names:	DDB_G0269204

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1699 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May act in a signal transduction pathway. This protein undergoes an ATP-dependent autophosphorylation at a conserved histidine residue in the kinase core, and a phosphoryl group is then transferred to a conserved aspartate residue in the receiver domain By similarity.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Contains 1 histidine kinase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Two-component regulatory system
Cellular component	Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transducer Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component response regulator activity Inferred from electronic annotation. Source: InterPro two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1699	1699	Hybrid signal transduction histidine kinase E		PRO_0000328271
Regions
	Transmembrane	142 – 162	21	Potential
	Transmembrane	164 – 184	21	Potential
	Transmembrane	191 – 211	21	Potential
	Transmembrane	238 – 258	21	Potential
	Transmembrane	262 – 282	21	Potential
	Transmembrane	295 – 315	21	Potential
	Domain	678 – 950	273	Histidine kinase
	Domain	1575 – 1695	121	Response regulatory
	Compositional bias	63 – 98	36	Poly-Asn
	Compositional bias	395 – 407	13	Poly-Gln
	Compositional bias	433 – 449	17	Poly-Asn
	Compositional bias	452 – 466	15	Poly-Asn
	Compositional bias	544 – 590	47	Poly-Asn
	Compositional bias	597 – 604	8	Poly-Asn
	Compositional bias	819 – 848	30	Poly-Asn
	Compositional bias	855 – 869	15	Poly-Asn
	Compositional bias	1013 – 1054	42	Poly-Asn
	Compositional bias	1188 – 1197	10	Poly-Gln
	Compositional bias	1355 – 1392	38	Poly-Asn
	Compositional bias	1428 – 1436	9	Poly-Ser
	Compositional bias	1493 – 1534	42	Poly-Gln
	Compositional bias	1541 – 1545	5	Poly-Gln
Amino acid modifications
	Modified residue	681	1	Phosphohistidine; by autocatalysis By similarity
	Modified residue	1625	1	4-aspartylphosphate By similarity
Experimental info
	Sequence conflict	665	1	A → T in AAK54094. Ref.1
	Sequence conflict	803	1	T → P in AAK54094. Ref.1
	Sequence conflict	813	1	S → T in AAK54094. Ref.1
	Sequence conflict	817	1	Q → P in AAK54094. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q55E44-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: ED9704160973EAE2	FASTA	1,699	191,750
	10 20 30 40 50 60 MDKLKINNNL SPPSSPSSST TTPNLSSTNL ENNLNSNINN NINNFNLNNS TNTFNNSNNI 70 80 90 100 110 120 IINNNNNNNN NNNNNNNNNN NNSNNNNNNI NNNNPNVNSP NEVILNNNFL KQNKKGFFKR 130 140 150 160 170 180 NKKMIEIFLP PKFKSPIYRR CCILLGECGV YVFIYMFFLI FLRSFYPIFI CGIVSMIVLY 190 200 210 220 230 240 IVSTKTTKYK LVALIYIFVQ SILNFTFFLQ ITNNFNNNTV NINNTDDYSN EVIEFSKLNF 250 260 270 280 290 300 LFIMNLILSL ISIQIFFPKF TFSITLTCSL NIFNIIIHLI SIYSLNTKKL IVFQNLIVPI 310 320 330 340 350 360 TVSFLLSFYS YILSIDNQEI EAKEKRFRNI FDTSSEALVI HKNGLIIDVN STFEKIFQIK 370 380 390 400 410 420 QSDLINGTIW EYLPELESFF QNTNGNSKYT NQSLQQQLQQ QQQQQQQLYN TITNGGNNKQ 430 440 450 460 470 480 TSTTSANSTP RYNNYNNNNI NSNNLNNLNV FNNLNNLNNL NNNNNNHHIH KVSRSNTGLS 490 500 510 520 530 540 DSSSIFDDSD SVDFFSMKNI NKCPIVLDTV GITPCGSEFC AQVKIERKKD MLIGSGTSSF 550 560 570 580 590 600 NLLNNNNNNN NNNNNNNNNN NNNNNNNNNS NNNNNNNSNN NNNNNNINNN ISNNIINNNN 610 620 630 640 650 660 NNNNSGIITN LSQQSNNLST TNLNTSSKII NHKHKNSKQD FDVMSIVDIS AKKKLMIADQ 670 680 690 700 710 720 ALRRAEELNQ AKINFLTTVS HEVRTPINGI LASVEILDGS QLDVTQRDFL SCIKQSADYL 730 740 750 760 770 780 LDLITDILDF SKIEAGKFEL DRVEFNLITM LEESINIVYR TAQERGIEVL TFIDPDVPII 790 800 810 820 830 840 LIGDPYRVKQ CVLNFLSNAI KFTHKGQVMV KVSIVDQINN NNNNNNNNNN NNNNNNNNNN 850 860 870 880 890 900 NNNNNNNNKI VGFSNNNNNN NNNNNNGNNG ILNFKLSFSV EDSGVGIKEE VLDCLFTPFH 910 920 930 940 950 960 QLGGSPRKYL GTGLGLSISK KLTTLMGGEI GVKSVYGVGS AFSFTSILST TSTTPISLQS 970 980 990 1000 1010 1020 LGSSLSIALN NLSPTIPKIS GFIYDDNIHT SNSLFNFLKL MNINLKIINP IINNNNNNNN 1030 1040 1050 1060 1070 1080 NNNNNNNNNN NNNNNNNNNN NDNNNNNNNN NDNNIDQQQL KFEFENEIDK YCNVEDDNNN 1090 1100 1110 1120 1130 1140 VILIITNQLS NDNLNYFKDK INQLNNSIYW FVLCDNGLKA IDPFYYGIVK KPNNLLNLVD 1150 1160 1170 1180 1190 1200 TVFKVYNCQL PQDLYQLLTN GSKDDYKNYL KYRNVVIKKI TEDNEKKQQQ QQQQQQQMGD 1210 1220 1230 1240 1250 1260 TLSSTKSPQY TNLPPLDISS SSNGSLNKSN RSNLLRKSSS VYSDHVAITR GMVQVNRSPR 1270 1280 1290 1300 1310 1320 PSTPPPLFNL KGNNSNPNST ELNSTNSVNG NPNNDSTLET IEPLNISETV GDLCSSGSVI 1330 1340 1350 1360 1370 1380 NNSNFIINNN FLTSSPYGSC LTPNSGTTSP SIPININNND NNNNNNNNNN NNNNNNNNNN 1390 1400 1410 1420 1430 1440 NNNNNNNNNN NNSFKKLEPD NFSPPPSILI NDNNIIPALN VTTPITISTS TSSSSSLLIN 1450 1460 1470 1480 1490 1500 NEINNKSTTS KRPSFIPPLD IQSKKLIINT ADENHKNNCI IDFEQIFGQD GKQQPQQQQQ 1510 1520 1530 1540 1550 1560 QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQKLNIGN QQQQQILSSP RLQSQLLSNC 1570 1580 1590 1600 1610 1620 DSLGNTPPVT PHRRNALIVD DTELNRKVLA QLLRRMDWSV SFAENGIEAL KEITSERCFD 1630 1640 1650 1660 1670 1680 IIFMDCQMPI LDGFETTKLL RLRELENNWK PLNIVALSAD SSSSFGQVCF DCGMNGYLGK 1690 PITLITLKDT LLKWGGYRD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The histidine kinases of Dictyostelium." Anjard C., Loomis W.F. (In) Inouye M., Dutta R. (eds.); Histidine kinases in signal transduction, pp.1-1, Academic press, San Diego (2001) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF362375 Genomic DNA. Translation: AAK54094.2. AAFI02000005 Genomic DNA. Translation: EAL71952.1.
RefSeq	XP_645937.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3396982.
GenomeReviews	Gene locus dhkE in contig CM000150_GR.
KEGG	ddi:DDB_0191265.
Organism-specific databases
dictyBase	DDB_G0269204. dhkE.
Enzyme and pathway databases
BRENDA	2.7.13.3. 424.
Family and domain databases
InterPro	IPR003594. ATP_bd_ATPase. IPR000014. PAS. IPR013767. PAS_fold. IPR004358. Sig_transdc_His_kin-like_C. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
Pfam	PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. PF00989. PAS. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
ProDom	PD000039. Response_reg. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. SM00091. PAS. 1 hit. SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50109. HIS_KIN. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHKE_DICDI

Accession

Primary (citable) accession number: Q55E44
Secondary accession number(s): Q95PH3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	October 13, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)