Reviewed, UniProtKB/Swiss-Prot Q55E32 (EBP_DICDI)

Names and origin

Protein names

Recommended name:
    Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase
    EC=5.3.3.5
Alternative name(s):
    Cholestenol Delta-isomerase
    Delta(8)-Delta(7) sterol isomerase
      Short name=D8-D7 sterol isomerase
    Emopamil-binding protein homolog

Gene names

Name:	ebp
ORF Names:	DDB_G0269414

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the conversion of Delta(8)-sterols to their corresponding Delta(7)-isomers By similarity.
Catalytic activity	5-alpha-cholest-7-en-3-beta-ol = 5-alpha-cholest-8-en-3-beta-ol.
Pathway	Steroid biosynthesis; cholesterol biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the EBP family.

Ontologies

Keywords
Biological process	Cholesterol biosynthesis Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cholesterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cholestenol delta-isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 219	219	Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase		PRO_0000331293
Regions
	Transmembrane	3 – 23	21	Potential
	Transmembrane	30 – 50	21	Potential
	Transmembrane	101 – 121	21	Potential
	Transmembrane	135 – 155	21	Potential
	Transmembrane	169 – 189	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q55E32-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 86DA1F19D2AEB6FC	FASTA	219	25,195
	10 20 30 40 50 60 MEIFAFVPLA VLTALCVVIS LFVKREKLVV FWLLWSGLIH IILEGSYGFF AHEVTKASTV 70 80 90 100 110 120 SFTEKMLELV PLENAWNPHW YAQLYSQYAK YDLRYAIQDP MVVFFCFLEL VQGAACFLLV 130 140 150 160 170 180 ICVIKQARIR HALQIFLCSI QGLGTVFYFI TPYIYGLWEQ QISSDPFELW VYVVGLNGLW 190 200 210 LLVPIILTIQ SFIAINKQFS IVESTTKDAK FISRKPKTA

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000005 Genomic DNA. Translation: EAL72057.1.
RefSeq	XP_645949.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3397087.
GenomeReviews	Gene locus ebp in contig CM000150_GR.
KEGG	ddi:DDBDRAFT_0190243.
Organism-specific databases
dictyBase	DDB_G0269414. ebp.
Phylogenomic databases
OMA	HYSANET.
Family and domain databases
InterPro	IPR007905. EBP. [Graphical view]
PANTHER	PTHR14207. EBP. 1 hit.
Pfam	PF05241. EBP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EBP_DICDI

Accession

Primary (citable) accession number: Q55E32

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	May 24, 2005
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)