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Protein

Delta-aminolevulinic acid dehydratase

Gene

alad

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen (By similarity).By similarity

Catalytic activityi

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per monomer.By similarity

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Delta-aminolevulinic acid dehydratase (alad)
  2. Porphobilinogen deaminase (hemC)
  3. no protein annotated in this organism
  4. Uroporphyrinogen decarboxylase (hemE)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi127Zinc; catalyticBy similarity1
Metal bindingi129Zinc; catalyticBy similarity1
Metal bindingi137Zinc; catalyticBy similarity1
Active sitei204Schiff-base intermediate with substrateBy similarity1
Binding sitei214Substrate 1By similarity1
Binding sitei226Substrate 1By similarity1
Active sitei257Schiff-base intermediate with substrateBy similarity1
Binding sitei283Substrate 2By similarity1
Binding sitei322Substrate 2By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processHeme biosynthesis, Porphyrin biosynthesis
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DDI-189451 Heme biosynthesis
R-DDI-6798695 Neutrophil degranulation
UniPathwayiUPA00251; UER00318

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
Short name:
ALADH
Alternative name(s):
Porphobilinogen synthase
Gene namesi
Name:alad
Synonyms:hemB
ORF Names:DDB_G0269444
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 1, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0269444 hemB

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003280391 – 333Delta-aminolevulinic acid dehydrataseAdd BLAST333

Proteomic databases

PaxDbiQ55E06
PRIDEiQ55E06

Interactioni

Subunit structurei

Homooctamer.By similarity

Protein-protein interaction databases

STRINGi44689.DDB0231415

Structurei

3D structure databases

ProteinModelPortaliQ55E06
SMRiQ55E06
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ALAD family.Curated

Phylogenomic databases

eggNOGiKOG2794 Eukaryota
COG0113 LUCA
InParanoidiQ55E06
KOiK01698
OMAiYQMDYAN
PhylomeDBiQ55E06

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR001731 ALAD
IPR030656 ALAD_AS
IPR013785 Aldolase_TIM
PANTHERiPTHR11458 PTHR11458, 1 hit
PfamiView protein in Pfam
PF00490 ALAD, 1 hit
PIRSFiPIRSF001415 Porphbilin_synth, 1 hit
PRINTSiPR00144 DALDHYDRTASE
SMARTiView protein in SMART
SM01004 ALAD, 1 hit
PROSITEiView protein in PROSITE
PS00169 D_ALA_DEHYDRATASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q55E06-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENNNKNYSH YLHSGYAHPI TRSWQGDKVI LKSQLIYPIF VTDLINTKTE
60 70 80 90 100
IKSLPGQYQI SSDLVVEFLR PLVEKGLKSI ILFGVIISGV KDEQASSADK
110 120 130 140 150
RESSPVIKSI ELIKNEFPEI LICTDLCLCA YTDHGHCGVL TEEGFIENEK
160 170 180 190 200
SIIRLGEIAL SFAKAGAHVI APSDMMDCRV GQIKKVLFQN GYGGRVAVMA
210 220 230 240 250
YSSKFASSYY GPFRDAAGSG AKHGDRQAYQ LPIASRGLGL RAALRDEAEG
260 270 280 290 300
ADFIMVKPAG PYMDIIREVK DHVKVPVCCY QVSGEYAMIY HASVAGGIDL
310 320 330
KSGVMESLIS LQRSGCDIFI TYFTPQLLDW LKL
Length:333
Mass (Da):36,744
Last modified:May 2, 2006 - v2
Checksum:i6ADA07F1C4933E5F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000005 Genomic DNA Translation: EAL72072.2
RefSeqiXP_645975.2, XM_640883.2

Genome annotation databases

EnsemblProtistsiEAL72072; EAL72072; DDB_G0269444
GeneIDi8616919
KEGGiddi:DDB_G0269444

Similar proteinsi

Entry informationi

Entry nameiHEM2_DICDI
AccessioniPrimary (citable) accession number: Q55E06
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 2, 2006
Last modified: May 23, 2018
This is version 89 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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