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Q55E06

- HEM2_DICDI

UniProt

Q55E06 - HEM2_DICDI

Protein

Delta-aminolevulinic acid dehydratase

Gene

alad

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 2 (02 May 2006)
      Previous versions | rss
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    Functioni

    Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.By similarity

    Catalytic activityi

    2 5-aminolevulinate = porphobilinogen + 2 H2O.

    Cofactori

    Binds 1 zinc ion per monomer.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi127 – 1271Zinc; catalyticBy similarity
    Metal bindingi129 – 1291Zinc; catalyticBy similarity
    Metal bindingi137 – 1371Zinc; catalyticBy similarity
    Active sitei204 – 2041Schiff-base intermediate with substrateBy similarity
    Binding sitei214 – 2141Substrate 1By similarity
    Binding sitei226 – 2261Substrate 1By similarity
    Active sitei257 – 2571Schiff-base intermediate with substrateBy similarity
    Binding sitei283 – 2831Substrate 2By similarity
    Binding sitei322 – 3221Substrate 2By similarity

    GO - Molecular functioni

    1. lead ion binding Source: UniProtKB
    2. porphobilinogen synthase activity Source: UniProtKB
    3. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. heme biosynthetic process Source: UniProtKB
    2. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Heme biosynthesis, Porphyrin biosynthesis

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_204058. Heme biosynthesis.
    UniPathwayiUPA00251; UER00318.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
    Short name:
    ALADH
    Alternative name(s):
    Porphobilinogen synthase
    Gene namesi
    Name:alad
    Synonyms:hemB
    ORF Names:DDB_G0269444
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 1, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0269444. hemB.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 333333Delta-aminolevulinic acid dehydratasePRO_0000328039Add
    BLAST

    Proteomic databases

    PRIDEiQ55E06.

    Interactioni

    Subunit structurei

    Homooctamer.By similarity

    Protein-protein interaction databases

    STRINGi44689.DDB_0231415.

    Structurei

    3D structure databases

    ProteinModelPortaliQ55E06.
    SMRiQ55E06. Positions 9-332.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ALADH family.Curated

    Phylogenomic databases

    eggNOGiCOG0113.
    KOiK01698.
    OMAiVSRAMSY.
    PhylomeDBiQ55E06.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR001731. Porphobilinogen_synth.
    [Graphical view]
    PANTHERiPTHR11458. PTHR11458. 1 hit.
    PfamiPF00490. ALAD. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
    PRINTSiPR00144. DALDHYDRTASE.
    SMARTiSM01004. ALAD. 1 hit.
    [Graphical view]
    PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q55E06-1 [UniParc]FASTAAdd to Basket

    « Hide

    MENNNKNYSH YLHSGYAHPI TRSWQGDKVI LKSQLIYPIF VTDLINTKTE    50
    IKSLPGQYQI SSDLVVEFLR PLVEKGLKSI ILFGVIISGV KDEQASSADK 100
    RESSPVIKSI ELIKNEFPEI LICTDLCLCA YTDHGHCGVL TEEGFIENEK 150
    SIIRLGEIAL SFAKAGAHVI APSDMMDCRV GQIKKVLFQN GYGGRVAVMA 200
    YSSKFASSYY GPFRDAAGSG AKHGDRQAYQ LPIASRGLGL RAALRDEAEG 250
    ADFIMVKPAG PYMDIIREVK DHVKVPVCCY QVSGEYAMIY HASVAGGIDL 300
    KSGVMESLIS LQRSGCDIFI TYFTPQLLDW LKL 333
    Length:333
    Mass (Da):36,744
    Last modified:May 2, 2006 - v2
    Checksum:i6ADA07F1C4933E5F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000005 Genomic DNA. Translation: EAL72072.2.
    RefSeqiXP_645975.2. XM_640883.2.

    Genome annotation databases

    EnsemblProtistsiDDB0231415; DDB0231415; DDB_G0269444.
    GeneIDi8616919.
    KEGGiddi:DDB_G0269444.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000005 Genomic DNA. Translation: EAL72072.2 .
    RefSeqi XP_645975.2. XM_640883.2.

    3D structure databases

    ProteinModelPortali Q55E06.
    SMRi Q55E06. Positions 9-332.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0231415.

    Proteomic databases

    PRIDEi Q55E06.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0231415 ; DDB0231415 ; DDB_G0269444 .
    GeneIDi 8616919.
    KEGGi ddi:DDB_G0269444.

    Organism-specific databases

    dictyBasei DDB_G0269444. hemB.

    Phylogenomic databases

    eggNOGi COG0113.
    KOi K01698.
    OMAi VSRAMSY.
    PhylomeDBi Q55E06.

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00318 .
    Reactomei REACT_204058. Heme biosynthesis.

    Miscellaneous databases

    PROi Q55E06.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR001731. Porphobilinogen_synth.
    [Graphical view ]
    PANTHERi PTHR11458. PTHR11458. 1 hit.
    Pfami PF00490. ALAD. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001415. Porphbilin_synth. 1 hit.
    PRINTSi PR00144. DALDHYDRTASE.
    SMARTi SM01004. ALAD. 1 hit.
    [Graphical view ]
    PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiHEM2_DICDI
    AccessioniPrimary (citable) accession number: Q55E06
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: May 2, 2006
    Last modified: October 1, 2014
    This is version 64 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3