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Q55E06

- HEM2_DICDI

UniProt

Q55E06 - HEM2_DICDI

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Protein
Delta-aminolevulinic acid dehydratase
Gene
alad, hemB, DDB_G0269444
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.

Catalytic activityi

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Cofactori

Binds 1 zinc ion per monomer By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi127 – 1271Zinc; catalytic By similarity
Metal bindingi129 – 1291Zinc; catalytic By similarity
Metal bindingi137 – 1371Zinc; catalytic By similarity
Active sitei204 – 2041Schiff-base intermediate with substrate By similarity
Binding sitei214 – 2141Substrate 1 By similarity
Binding sitei226 – 2261Substrate 1 By similarity
Active sitei257 – 2571Schiff-base intermediate with substrate By similarity
Binding sitei283 – 2831Substrate 2 By similarity
Binding sitei322 – 3221Substrate 2 By similarity

GO - Molecular functioni

  1. lead ion binding Source: UniProtKB
  2. porphobilinogen synthase activity Source: UniProtKB
  3. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. heme biosynthetic process Source: UniProtKB
  2. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_204058. Heme biosynthesis.
UniPathwayiUPA00251; UER00318.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
Short name:
ALADH
Alternative name(s):
Porphobilinogen synthase
Gene namesi
Name:alad
Synonyms:hemB
ORF Names:DDB_G0269444
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 1, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0269444. hemB.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 333333Delta-aminolevulinic acid dehydratase
PRO_0000328039Add
BLAST

Proteomic databases

PRIDEiQ55E06.

Interactioni

Subunit structurei

Homooctamer By similarity.

Protein-protein interaction databases

STRINGi44689.DDB_0231415.

Structurei

3D structure databases

ProteinModelPortaliQ55E06.
SMRiQ55E06. Positions 9-332.

Family & Domainsi

Sequence similaritiesi

Belongs to the ALADH family.

Phylogenomic databases

eggNOGiCOG0113.
KOiK01698.
OMAiVSRAMSY.
PhylomeDBiQ55E06.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view]
PANTHERiPTHR11458. PTHR11458. 1 hit.
PfamiPF00490. ALAD. 1 hit.
[Graphical view]
PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
PRINTSiPR00144. DALDHYDRTASE.
SMARTiSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q55E06-1 [UniParc]FASTAAdd to Basket

« Hide

MENNNKNYSH YLHSGYAHPI TRSWQGDKVI LKSQLIYPIF VTDLINTKTE    50
IKSLPGQYQI SSDLVVEFLR PLVEKGLKSI ILFGVIISGV KDEQASSADK 100
RESSPVIKSI ELIKNEFPEI LICTDLCLCA YTDHGHCGVL TEEGFIENEK 150
SIIRLGEIAL SFAKAGAHVI APSDMMDCRV GQIKKVLFQN GYGGRVAVMA 200
YSSKFASSYY GPFRDAAGSG AKHGDRQAYQ LPIASRGLGL RAALRDEAEG 250
ADFIMVKPAG PYMDIIREVK DHVKVPVCCY QVSGEYAMIY HASVAGGIDL 300
KSGVMESLIS LQRSGCDIFI TYFTPQLLDW LKL 333
Length:333
Mass (Da):36,744
Last modified:May 2, 2006 - v2
Checksum:i6ADA07F1C4933E5F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000005 Genomic DNA. Translation: EAL72072.2.
RefSeqiXP_645975.2. XM_640883.2.

Genome annotation databases

EnsemblProtistsiDDB0231415; DDB0231415; DDB_G0269444.
GeneIDi8616919.
KEGGiddi:DDB_G0269444.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000005 Genomic DNA. Translation: EAL72072.2 .
RefSeqi XP_645975.2. XM_640883.2.

3D structure databases

ProteinModelPortali Q55E06.
SMRi Q55E06. Positions 9-332.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0231415.

Proteomic databases

PRIDEi Q55E06.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0231415 ; DDB0231415 ; DDB_G0269444 .
GeneIDi 8616919.
KEGGi ddi:DDB_G0269444.

Organism-specific databases

dictyBasei DDB_G0269444. hemB.

Phylogenomic databases

eggNOGi COG0113.
KOi K01698.
OMAi VSRAMSY.
PhylomeDBi Q55E06.

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00318 .
Reactomei REACT_204058. Heme biosynthesis.

Miscellaneous databases

PROi Q55E06.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view ]
PANTHERi PTHR11458. PTHR11458. 1 hit.
Pfami PF00490. ALAD. 1 hit.
[Graphical view ]
PIRSFi PIRSF001415. Porphbilin_synth. 1 hit.
PRINTSi PR00144. DALDHYDRTASE.
SMARTi SM01004. ALAD. 1 hit.
[Graphical view ]
PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiHEM2_DICDI
AccessioniPrimary (citable) accession number: Q55E06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 2, 2006
Last modified: September 3, 2014
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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