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Protein

Delta-aminolevulinic acid dehydratase

Gene

alad

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen (By similarity).By similarity

Catalytic activityi

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per monomer.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi127 – 1271Zinc; catalyticBy similarity
Metal bindingi129 – 1291Zinc; catalyticBy similarity
Metal bindingi137 – 1371Zinc; catalyticBy similarity
Active sitei204 – 2041Schiff-base intermediate with substrateBy similarity
Binding sitei214 – 2141Substrate 1By similarity
Binding sitei226 – 2261Substrate 1By similarity
Active sitei257 – 2571Schiff-base intermediate with substrateBy similarity
Binding sitei283 – 2831Substrate 2By similarity
Binding sitei322 – 3221Substrate 2By similarity

GO - Molecular functioni

  1. lead ion binding Source: UniProtKB
  2. porphobilinogen synthase activity Source: UniProtKB
  3. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. heme biosynthetic process Source: UniProtKB
  2. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00251; UER00318.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
Short name:
ALADH
Alternative name(s):
Porphobilinogen synthase
Gene namesi
Name:alad
Synonyms:hemB
ORF Names:DDB_G0269444
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 1, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0269444. hemB.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 333333Delta-aminolevulinic acid dehydratasePRO_0000328039Add
BLAST

Proteomic databases

PRIDEiQ55E06.

Interactioni

Subunit structurei

Homooctamer.By similarity

Protein-protein interaction databases

STRINGi44689.DDB_0231415.

Structurei

3D structure databases

ProteinModelPortaliQ55E06.
SMRiQ55E06. Positions 9-332.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ALAD family.Curated

Phylogenomic databases

eggNOGiCOG0113.
InParanoidiQ55E06.
KOiK01698.
OMAiADCIITY.
PhylomeDBiQ55E06.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view]
PANTHERiPTHR11458. PTHR11458. 1 hit.
PfamiPF00490. ALAD. 1 hit.
[Graphical view]
PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
PRINTSiPR00144. DALDHYDRTASE.
SMARTiSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q55E06-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENNNKNYSH YLHSGYAHPI TRSWQGDKVI LKSQLIYPIF VTDLINTKTE
60 70 80 90 100
IKSLPGQYQI SSDLVVEFLR PLVEKGLKSI ILFGVIISGV KDEQASSADK
110 120 130 140 150
RESSPVIKSI ELIKNEFPEI LICTDLCLCA YTDHGHCGVL TEEGFIENEK
160 170 180 190 200
SIIRLGEIAL SFAKAGAHVI APSDMMDCRV GQIKKVLFQN GYGGRVAVMA
210 220 230 240 250
YSSKFASSYY GPFRDAAGSG AKHGDRQAYQ LPIASRGLGL RAALRDEAEG
260 270 280 290 300
ADFIMVKPAG PYMDIIREVK DHVKVPVCCY QVSGEYAMIY HASVAGGIDL
310 320 330
KSGVMESLIS LQRSGCDIFI TYFTPQLLDW LKL
Length:333
Mass (Da):36,744
Last modified:May 2, 2006 - v2
Checksum:i6ADA07F1C4933E5F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000005 Genomic DNA. Translation: EAL72072.2.
RefSeqiXP_645975.2. XM_640883.2.

Genome annotation databases

EnsemblProtistsiDDB0231415; DDB0231415; DDB_G0269444.
GeneIDi8616919.
KEGGiddi:DDB_G0269444.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000005 Genomic DNA. Translation: EAL72072.2.
RefSeqiXP_645975.2. XM_640883.2.

3D structure databases

ProteinModelPortaliQ55E06.
SMRiQ55E06. Positions 9-332.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB_0231415.

Proteomic databases

PRIDEiQ55E06.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0231415; DDB0231415; DDB_G0269444.
GeneIDi8616919.
KEGGiddi:DDB_G0269444.

Organism-specific databases

dictyBaseiDDB_G0269444. hemB.

Phylogenomic databases

eggNOGiCOG0113.
InParanoidiQ55E06.
KOiK01698.
OMAiADCIITY.
PhylomeDBiQ55E06.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00318.

Miscellaneous databases

PROiQ55E06.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view]
PANTHERiPTHR11458. PTHR11458. 1 hit.
PfamiPF00490. ALAD. 1 hit.
[Graphical view]
PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
PRINTSiPR00144. DALDHYDRTASE.
SMARTiSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiHEM2_DICDI
AccessioniPrimary (citable) accession number: Q55E06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 2, 2006
Last modified: January 7, 2015
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.