Q55DZ8 (SYWC_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan--tRNA ligase, cytoplasmic
EC=6.1.1.2

Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS

Gene names

Name:	trpS
ORF Names:	DDB_G0269454

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	translation Inferred from sequence or structural similarity. Source: dictyBase tryptophanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from sequence or structural similarity. Source: dictyBase
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tryptophan-tRNA ligase activity Inferred from sequence or structural similarity. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 400	400	Tryptophan--tRNA ligase, cytoplasmic		PRO_0000327792
Regions
	Motif	95 – 104	10	"HIGH" region
	Motif	281 – 285	5	"KMSKS" region

Sequences

	Sequence		Length	Mass (Da)
	Q55DZ8 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 38FC775E37AE342C	FASTA	400	45,850
	10 20 30 40 50 60 MTETTPTTNE TKEQVITPWE VEAAPGGSVD YMKLVDQFGS TVISEELIAR FEKVTGKRAH 70 80 90 100 110 120 HFLRRGIFFS HRDLKEILDH HESGKKWFLY TGRGPSSGSL HFGHLLPFTF TKYLQDAFNV 130 140 150 160 170 180 PLVVQMTNDE KFLWKDMTLE ESIKFTHNNV KDIIALGFDI QKTFIFSNLE YIHHLYPNVL 190 200 210 220 230 240 KIARCVNLNQ IQNIFGFKES DAIGKFTFPP VQAAPCFPDS FPHIFPLNDP EIKNIRCLIP 250 260 270 280 290 300 CAIDQDPYFR MTRDIAHRIG HQKPALIHSK FFPALQGHNT KMSASDTNSA VYLSDTPDQV 310 320 330 340 350 360 KDKIKKHAFS GGGATKEEQE KNGADLSVDI TYEYLTFMLE DDEQLKDIAH RYSTGKMMTG 370 380 390 400 EIKQILIDLM NKIIIRHKEA RAKITDEVLS TFMSIRKLNF

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000005 Genomic DNA. Translation: EAL72077.1.
RefSeq	XP_645983.1. XM_640891.1.
3D structure databases
HSSP	HSSP built from PDB template 1ULH based on UniProtKB P23381.
ProteinModelPortal	Q55DZ8.
SMR	Q55DZ8. Positions 28-400.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0231245.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231245; DDB0231245; DDB_G0269454.
GeneID	8616928.
KEGG	ddi:DDB_G0269454.
Organism-specific databases
dictyBase	DDB_G0269454. trpS.
Phylogenomic databases
eggNOG	COG0180.
KO	K01867.
OMA	KTGGRET.
ProtClustDB	CLSZ2729284.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
InterPro	IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002306. Trp-tRNA-ligase. [Graphical view]
PANTHER	PTHR10055. PTHR10055. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01039. TRNASYNTHTRP.
TIGRFAMs	TIGR00233. trpS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYWC_DICDI

Accession

Primary (citable) accession number: Q55DZ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	May 1, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)