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Protein

Cystathionine gamma-lyase

Gene

cysA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-cystathionine + H2O = L-cysteine + NH3 + 2-oxobutanoate.

Cofactori

Pathwayi: L-cysteine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-cysteine from L-homocysteine and L-serine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Cystathionine beta-synthase (cysB)
  2. Cystathionine gamma-lyase (cysA)
This subpathway is part of the pathway L-cysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cysteine from L-homocysteine and L-serine, the pathway L-cysteine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei53 – 531SubstrateBy similarity
Binding sitei105 – 1051SubstrateBy similarity
Binding sitei110 – 1101SubstrateBy similarity
Binding sitei329 – 3291SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiR-DDI-1614558. Degradation of cysteine and homocysteine.
R-DDI-1614603. Cysteine formation from homocysteine.
UniPathwayiUPA00136; UER00202.

Names & Taxonomyi

Protein namesi
Recommended name:
Cystathionine gamma-lyase (EC:4.4.1.1)
Alternative name(s):
Gamma-cystathionase
Gene namesi
Name:cysA
ORF Names:DDB_G0269122
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 1, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0269122. cysA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 387387Cystathionine gamma-lyasePRO_0000327889Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei202 – 2021N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

PaxDbiQ55DV9.
PRIDEiQ55DV9.

Expressioni

Developmental stagei

Up-regulated in aspidocytes, a resistant cell type induced by a range of toxins including heavy metals and antibiotics.1 Publication

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi44689.DDB0191318.

Structurei

3D structure databases

ProteinModelPortaliQ55DV9.
SMRiQ55DV9. Positions 4-384.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the trans-sulfuration enzymes family.Curated

Phylogenomic databases

eggNOGiKOG0053. Eukaryota.
COG0626. LUCA.
InParanoidiQ55DV9.
KOiK01758.
OMAiSPIDCYL.
PhylomeDBiQ55DV9.

Family and domain databases

CDDicd00614. CGS_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 1 hit.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
PIRSFiPIRSF001434. CGS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.

Sequencei

Sequence statusi: Complete.

Q55DV9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQPNNYKIG TNVIHAGQSA DKNTGAVIVP ISLSTTFLQP SPGVLHSEYD
60 70 80 90 100
YSRSGNPTRK AFEECIAACE NAKYALSFAS GLATLTTITH LLKSGDEVIS
110 120 130 140 150
IDDVYGGTRR YFTRVAANFD LKFSFVDLST LDDLKNAFTD KTRLVWIETP
160 170 180 190 200
TNPLLKVADI KAVADYVHSR GATLVVDNTF MSPYFQNPLD LGADIVMHSV
210 220 230 240 250
TKYINGHSDC VMGVLATNND ELYAKLKFLQ NSIGAVPSPF DCFLALRGLK
260 270 280 290 300
TLHVRMEAHQ KNAFAICNFL EKHPKVERVI YPGLPSHPQH EICKRQMKGY
310 320 330 340 350
GGMVVFFVKG SIDQSRSFLE NIKLFALAES LGGVESLIEL PSVMTHASVP
360 370 380
AEERAKLGIS DTLIRLSVGI EDINDLLADI SQALDKC
Length:387
Mass (Da):42,497
Last modified:May 24, 2005 - v1
Checksum:i1A0D7EF1FE502434
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000005 Genomic DNA. Translation: EAL71911.1.
RefSeqiXP_646022.1. XM_640930.1.

Genome annotation databases

EnsemblProtistsiDDB0191318; DDB0191318; DDB_G0269122.
GeneIDi8616970.
KEGGiddi:DDB_G0269122.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000005 Genomic DNA. Translation: EAL71911.1.
RefSeqiXP_646022.1. XM_640930.1.

3D structure databases

ProteinModelPortaliQ55DV9.
SMRiQ55DV9. Positions 4-384.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0191318.

Proteomic databases

PaxDbiQ55DV9.
PRIDEiQ55DV9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0191318; DDB0191318; DDB_G0269122.
GeneIDi8616970.
KEGGiddi:DDB_G0269122.

Organism-specific databases

dictyBaseiDDB_G0269122. cysA.

Phylogenomic databases

eggNOGiKOG0053. Eukaryota.
COG0626. LUCA.
InParanoidiQ55DV9.
KOiK01758.
OMAiSPIDCYL.
PhylomeDBiQ55DV9.

Enzyme and pathway databases

UniPathwayiUPA00136; UER00202.
ReactomeiR-DDI-1614558. Degradation of cysteine and homocysteine.
R-DDI-1614603. Cysteine formation from homocysteine.

Miscellaneous databases

PROiQ55DV9.

Family and domain databases

CDDicd00614. CGS_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 1 hit.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
PIRSFiPIRSF001434. CGS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCGL_DICDI
AccessioniPrimary (citable) accession number: Q55DV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: September 7, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.