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Q55DR6

- FCSA_DICDI

UniProt

Q55DR6 - FCSA_DICDI

Protein

Fatty acyl-CoA synthetase A

Gene

fcsA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
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    Functioni

    Long chain fatty acid acyl-CoA synthetases catalyze the formation of a thiester bond between a free fatty acid and coenzyme A during fatty acid metabolic process. May mediate fatty acid retrieval from the lumen of endosomes into the cytoplasm.1 Publication

    Catalytic activityi

    ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. long-chain fatty acid-CoA ligase activity Source: dictyBase

    GO - Biological processi

    1. fatty acid metabolic process Source: dictyBase
    2. long-chain fatty acid metabolic process Source: GOC

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fatty acyl-CoA synthetase A (EC:6.2.1.3)
    Alternative name(s):
    Long chain fatty acyl coenzyme A-synthetase 1
    Short name:
    LC-FACS 1
    Long-chain-fatty-acid--CoA synthetase 1
    Gene namesi
    Name:fcsA
    ORF Names:DDB_G0269242
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 1, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0269242. fcsA.

    Subcellular locationi

    Endosome membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
    Note: Associates with endosomes a few minutes after their formation, remains bound through the acidic phase of endocytic maturation and dissociates early in the phase where the endosomal content is neutralized prior to exocytosis.

    GO - Cellular componenti

    1. cytoplasm Source: dictyBase
    2. cytoplasmic side of endosome membrane Source: dictyBase
    3. phagocytic vesicle Source: dictyBase

    Keywords - Cellular componenti

    Endosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 667667Fatty acyl-CoA synthetase APRO_0000328558Add
    BLAST

    Proteomic databases

    PRIDEiQ55DR6.

    Expressioni

    Developmental stagei

    Expressed at all stages of development.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0191105.

    Structurei

    3D structure databases

    ProteinModelPortaliQ55DR6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1022.
    KOiK01897.
    OMAiPRRNTKF.
    PhylomeDBiQ55DR6.

    Family and domain databases

    InterProiIPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view]
    PfamiPF00501. AMP-binding. 1 hit.
    [Graphical view]
    PROSITEiPS00455. AMP_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q55DR6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSLSTKTDL LGDPDFIRLQ SVEVDGSEVI PGETRPRRNT KFPKLTNSPD    50
    GKTFTLYDVY RINKDSDSNF LGIRELLADG KRGDYKWISY KQACIRANNI 100
    GSALVQLGLN KGDRIGIFSI NRPEWVLSDM AAMNHSLVPV ALYATLGANA 150
    IEYVVNHSEI SVLLCEGKNV EKILSMPGTT IKTIVSYDPL PQATLDKFKD 200
    NENVKLYLLS DFEKLGEQNP AQHEVPSPED LCTLLYTSGS TGNPKGVMLT 250
    HTNMVSEVAG ANFSPAGVIP EDVHMSYLPL AHSFERAVVS LMCYVGGQIG 300
    FFSGLIPELF NDIQVLRPTF LCGAPRVWQR LHDKLWFTVN NDSWLKKFLF 350
    NWGLNSKQSA LRLGSTTPIW DKLVFSKTKD RLGGRVKFIL SGSAPLDPKL 400
    AEFLRACFCC PVVSGYGLSE NVGGASVAYP EDNNVGHVGP PLSACEMKLI 450
    DVPEMNYFST DKPCPRGEVC IRGFNVFKGY FKDPEKTKED LKEDGWFHTG 500
    DIGRWNENGT LSIIDRKKNI FKLSQGEYVA AEYLESVFVR SPFASQVFVY 550
    GDSLNSFLVG VVVPDFEVVQ KLFASKYPEL DVSNHATLAK SKELYKEILS 600
    SFDACAAEAK LHGFEKLKHI YVEHEPFTEE NNLLTPSFKP KRPQLKERYQ 650
    TIIDTLYAEY KRDHPDV 667
    Length:667
    Mass (Da):74,609
    Last modified:May 24, 2005 - v1
    Checksum:i3F7A9C4B4AFBB2B4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti199 – 1991K → T in AAO43007. (PubMed:14629118)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY196478 mRNA. Translation: AAO43007.1.
    AAFI02000005 Genomic DNA. Translation: EAL71971.1.
    RefSeqiXP_646065.1. XM_640973.1.

    Genome annotation databases

    EnsemblProtistsiDDB0191105; DDB0191105; DDB_G0269242.
    GeneIDi8617013.
    KEGGiddi:DDB_G0269242.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY196478 mRNA. Translation: AAO43007.1 .
    AAFI02000005 Genomic DNA. Translation: EAL71971.1 .
    RefSeqi XP_646065.1. XM_640973.1.

    3D structure databases

    ProteinModelPortali Q55DR6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0191105.

    Proteomic databases

    PRIDEi Q55DR6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0191105 ; DDB0191105 ; DDB_G0269242 .
    GeneIDi 8617013.
    KEGGi ddi:DDB_G0269242.

    Organism-specific databases

    dictyBasei DDB_G0269242. fcsA.

    Phylogenomic databases

    eggNOGi COG1022.
    KOi K01897.
    OMAi PRRNTKF.
    PhylomeDBi Q55DR6.

    Miscellaneous databases

    PROi Q55DR6.

    Family and domain databases

    InterProi IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view ]
    Pfami PF00501. AMP-binding. 1 hit.
    [Graphical view ]
    PROSITEi PS00455. AMP_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A Dictyostelium long chain fatty acyl coenzyme A-synthetase mediates fatty acid retrieval from endosomes."
      von Loehneysen K., Pawolleck N., Ruehling H., Maniak M.
      Eur. J. Cell Biol. 82:505-514(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    3. "Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake."
      Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T.
      Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: AX2.

    Entry informationi

    Entry nameiFCSA_DICDI
    AccessioniPrimary (citable) accession number: Q55DR6
    Secondary accession number(s): Q86PL9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3