Reviewed, UniProtKB/Swiss-Prot Q55DR6 (FCSA_DICDI)

Names and origin

Protein names

Recommended name:
    Fatty acyl-CoA synthetase A
    EC=6.2.1.3
Alternative name(s):
    Long-chain-fatty-acid--CoA synthetase 1
    Long chain fatty acyl coenzyme A-synthetase 1
      Short name=LC-FACS 1

Gene names

Name:	fcsA
ORF Names:	DDB_G0269242

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	667 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Long chain fatty acid acyl-CoA synthetases catalyze the formation of a thiester bond between a free fatty acid and coenzyme A during fatty acid metabolic process. May mediate fatty acid retrieval from the lumen of endosomes into the cytoplasm. Ref.1
Catalytic activity	ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA.
Subcellular location	Endosome membrane; Peripheral membrane protein; Cytoplasmic side. Note: Associates with endosomes a few minutes after their formation, remains bound through the acidic phase of endocytic maturation and dissociates early in the phase where the endosomal content is neutralized prior to exocytosis. Ref.1
Developmental stage	Expressed at all stages of development. Ref.1
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Endosome Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid metabolic process Ref.1 Inferred from mutant phenotype. Source: dictyBase
Cellular component	external side of endosome membrane Ref.1 Inferred from direct assay. Source: dictyBase extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell phagocytic vesicle Ref.3 Inferred from direct assay. Source: dictyBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW long-chain-fatty-acid-CoA ligase activity Ref.1 Traceable author statement. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 667	667	Fatty acyl-CoA synthetase A		PRO_0000328558
Experimental info
	Sequence conflict	199	1	K → T in AAO43007. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q55DR6-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 3F7A9C4B4AFBB2B4	FASTA	667	74,609
	10 20 30 40 50 60 MSSLSTKTDL LGDPDFIRLQ SVEVDGSEVI PGETRPRRNT KFPKLTNSPD GKTFTLYDVY 70 80 90 100 110 120 RINKDSDSNF LGIRELLADG KRGDYKWISY KQACIRANNI GSALVQLGLN KGDRIGIFSI 130 140 150 160 170 180 NRPEWVLSDM AAMNHSLVPV ALYATLGANA IEYVVNHSEI SVLLCEGKNV EKILSMPGTT 190 200 210 220 230 240 IKTIVSYDPL PQATLDKFKD NENVKLYLLS DFEKLGEQNP AQHEVPSPED LCTLLYTSGS 250 260 270 280 290 300 TGNPKGVMLT HTNMVSEVAG ANFSPAGVIP EDVHMSYLPL AHSFERAVVS LMCYVGGQIG 310 320 330 340 350 360 FFSGLIPELF NDIQVLRPTF LCGAPRVWQR LHDKLWFTVN NDSWLKKFLF NWGLNSKQSA 370 380 390 400 410 420 LRLGSTTPIW DKLVFSKTKD RLGGRVKFIL SGSAPLDPKL AEFLRACFCC PVVSGYGLSE 430 440 450 460 470 480 NVGGASVAYP EDNNVGHVGP PLSACEMKLI DVPEMNYFST DKPCPRGEVC IRGFNVFKGY 490 500 510 520 530 540 FKDPEKTKED LKEDGWFHTG DIGRWNENGT LSIIDRKKNI FKLSQGEYVA AEYLESVFVR 550 560 570 580 590 600 SPFASQVFVY GDSLNSFLVG VVVPDFEVVQ KLFASKYPEL DVSNHATLAK SKELYKEILS 610 620 630 640 650 660 SFDACAAEAK LHGFEKLKHI YVEHEPFTEE NNLLTPSFKP KRPQLKERYQ TIIDTLYAEY KRDHPDV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A Dictyostelium long chain fatty acyl coenzyme A-synthetase mediates fatty acid retrieval from endosomes." von Loehneysen K., Pawolleck N., Ruehling H., Maniak M. Eur. J. Cell Biol. 82:505-514(2003) [PubMed: 14629118] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake." Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T. Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed: 16926386] [Abstract] Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY196478 mRNA. Translation: AAO43007.1. AAFI02000005 Genomic DNA. Translation: EAL71971.1.
RefSeq	XP_646065.1.
3D structure databases
HSSP	HSSP built from PDB template 1BA3 based on UniProtKB P08659.
SMR	Q55DR6. Positions 55-651.
ModBase	Search...
Protein-protein interaction databases
STRING	Q55DR6.
Genome annotation databases
GeneID	8617013.
KEGG	ddi:DDB_0191105.
Organism-specific databases
dictyBase	DDB_G0269242. fcsA.
Phylogenomic databases
eggNOG	KOG1256.
HOGENOM	HBG721674.
OMA	LTHANAV.
PhylomeDB	Q55DR6.
Enzyme and pathway databases
BRENDA	6.2.1.3. 424.
Family and domain databases
InterPro	IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FCSA_DICDI

Accession

Primary (citable) accession number: Q55DR6
Secondary accession number(s): Q86PL9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	February 9, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)