Reviewed, UniProtKB/Swiss-Prot Q55DP8 (ACY1_DICDI)

Names and origin

Protein names

Recommended name:
    Aminoacylase-1
    EC=3.5.1.14
Alternative name(s):
    N-acyl-L-amino-acid amidohydrolase
    ACY-1

Gene names

Name:	acy1
ORF Names:	DDB_G0270562

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	408 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate) By similarity.
Catalytic activity	An N-acyl-L-amino acid + H2O = a carboxylate + an L-amino acid.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase M20A family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminoacylase activity Inferred from electronic annotation. Source: EC metallopeptidase activity Inferred from electronic annotation. Source: InterPro protein dimerization activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 408	408	Aminoacylase-1		PRO_0000331214
Sites
	Active site	78	1	By similarity
	Active site	143	1	Proton acceptor By similarity
	Metal binding	76	1	Zinc 1 By similarity
	Metal binding	109	1	Zinc 1 By similarity
	Metal binding	109	1	Zinc 2 By similarity
	Metal binding	144	1	Zinc 2 By similarity
	Metal binding	172	1	Zinc 1 By similarity
	Metal binding	379	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q55DP8-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 8AD17E450D2FF224	FASTA	408	46,568
	10 20 30 40 50 60 MNSIQENEHV TVFREFLKIR TDHPTPDYES STKFLVEKAK EYNIPYEVYR ETGTPIVLMK 70 80 90 100 110 120 IEGLEPNLKT VLLNSHVDVV PAVHDSWKVD PFSAWKDESG NIFGRGTQDM KCVCMQFLEV 130 140 150 160 170 180 ARRIVQSGQK LKRTLHLSFV PDEEIGGSGK GMEKFVYTEK FRQLNIGLCL DEGLASPTND 190 200 210 220 230 240 FTVFYGERAP WWVHITAVGN AGHGSRFIEG TAIEKLMRTI NKMLAFRQEQ FESLHHGQHE 250 260 270 280 290 300 CGKKLGDVTS LNLTVLKAGI PIDHSNNFSY NVIPTQAEAG FDIRIPPTVN LDQFLDQIKE 310 320 330 340 350 360 WTAEEGLSFK FASYIPKNEM TKLDSDNKWW ENFKESCKKM DINLVTEIFP AATDSRFIRN 370 380 390 400 LGIPAFGFSP INNTPILLHD HNEFLNEKVY LRGIDIFMGI IPNLVNME

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000005 Genomic DNA. Translation: EAL72631.1.
RefSeq	XP_646083.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q55DP8.
Genome annotation databases
GeneID	3397661.
GenomeReviews	Gene locus acy1 in contig CM000150_GR.
KEGG	ddi:DDBDRAFT_0201767.
Organism-specific databases
dictyBase	DDB_G0270562. acy1.
Phylogenomic databases
OMA	KSVSIQY.
Family and domain databases
InterPro	IPR001261. ArgE/DapE_CS. IPR010159. N-acyl_aa_amidohydrolase. IPR002933. Peptidase_M20. IPR011650. Peptidase_M20_dimer. [Graphical view]
Pfam	PF07687. M20_dimer. 1 hit. PF01546. Peptidase_M20. 1 hit. [Graphical view]
PIRSF	PIRSF036696. ACY-1. 1 hit.
TIGRFAMs	TIGR01880. Ac-peptdase-euk. 1 hit.
PROSITE	PS00758. ARGE_DAPE_CPG2_1. 1 hit. PS00759. ARGE_DAPE_CPG2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACY1_DICDI

Accession

Primary (citable) accession number: Q55DP8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	May 24, 2005
Last modified:	November 3, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)