Q55DP8 (ACY1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aminoacylase-1
Short name=ACY-1
EC=3.5.1.14

Alternative name(s):
N-acyl-L-amino-acid amidohydrolase

Gene names

Name:	acy1
ORF Names:	DDB_G0270562

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	408 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate) By similarity.
Catalytic activity	An N-acyl-L-amino acid + H2O = a carboxylate + an L-amino acid.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase M20A family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aminoacylase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metallopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 408	408	Aminoacylase-1		PRO_0000331214
Sites
	Active site	78	1	By similarity
	Active site	143	1	Proton acceptor By similarity
	Metal binding	76	1	Zinc 1 By similarity
	Metal binding	109	1	Zinc 1 By similarity
	Metal binding	109	1	Zinc 2 By similarity
	Metal binding	144	1	Zinc 2 By similarity
	Metal binding	172	1	Zinc 1 By similarity
	Metal binding	379	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q55DP8 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 8AD17E450D2FF224	FASTA	408	46,568
	10 20 30 40 50 60 MNSIQENEHV TVFREFLKIR TDHPTPDYES STKFLVEKAK EYNIPYEVYR ETGTPIVLMK 70 80 90 100 110 120 IEGLEPNLKT VLLNSHVDVV PAVHDSWKVD PFSAWKDESG NIFGRGTQDM KCVCMQFLEV 130 140 150 160 170 180 ARRIVQSGQK LKRTLHLSFV PDEEIGGSGK GMEKFVYTEK FRQLNIGLCL DEGLASPTND 190 200 210 220 230 240 FTVFYGERAP WWVHITAVGN AGHGSRFIEG TAIEKLMRTI NKMLAFRQEQ FESLHHGQHE 250 260 270 280 290 300 CGKKLGDVTS LNLTVLKAGI PIDHSNNFSY NVIPTQAEAG FDIRIPPTVN LDQFLDQIKE 310 320 330 340 350 360 WTAEEGLSFK FASYIPKNEM TKLDSDNKWW ENFKESCKKM DINLVTEIFP AATDSRFIRN 370 380 390 400 LGIPAFGFSP INNTPILLHD HNEFLNEKVY LRGIDIFMGI IPNLVNME

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000005 Genomic DNA. Translation: EAL72631.1.
RefSeq	XP_646083.1. XM_640991.1.
3D structure databases
HSSP	HSSP built from PDB template 1Q7L based on UniProtKB Q03154.
ProteinModelPortal	Q55DP8.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDBDRAFT_0201767.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0266648; DDB0266648; DDB_G0270562.
GeneID	8617033.
KEGG	ddi:DDB_G0270562.
Organism-specific databases
dictyBase	DDB_G0270562. acy1.
Phylogenomic databases
eggNOG	COG0624.
KO	K14677.
OMA	KSEWENN.
ProtClustDB	CLSZ2431382.
Family and domain databases
InterPro	IPR001261. ArgE/DapE_CS. IPR010159. N-acyl_aa_amidohydrolase. IPR002933. Peptidase_M20. IPR011650. Peptidase_M20_dimer. [Graphical view]
Pfam	PF07687. M20_dimer. 1 hit. PF01546. Peptidase_M20. 1 hit. [Graphical view]
PIRSF	PIRSF036696. ACY-1. 1 hit.
SUPFAM	SSF55031. Peptidase_M20_dimer. 1 hit.
TIGRFAMs	TIGR01880. Ac-peptdase-euk. 1 hit.
PROSITE	PS00758. ARGE_DAPE_CPG2_1. 1 hit. PS00759. ARGE_DAPE_CPG2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACY1_DICDI

Accession

Primary (citable) accession number: Q55DP8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	May 24, 2005
Last modified:	May 1, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)