Q55DK5 (MGP3_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mental retardation GTPase activating protein homolog 3

Alternative name(s):
GTPase activating factor for raC protein AA
Rho GTPase-activating protein gacAA

Gene names

Name:	mgp3
Synonyms:	gacAA
ORF Names:	DDB_G0269624

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1337 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Rho GTPase-activating protein involved in the signal transduction pathway By similarity.
Subcellular location	Cytoplasm. Contractile vacuole By similarity.
Sequence similarities	Contains 1 FCH domain. Contains 1 Rho-GAP domain.

Ontologies

Keywords
Cellular component	Cytoplasm Vacuole
Domain	Coiled coil
Molecular function	GTPase activation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of GTPase activity Inferred from electronic annotation. Source: GOC signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	contractile vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTPase activator activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1337	1337	Mental retardation GTPase activating protein homolog 3		PRO_0000380226
Regions
	Domain	9 – 88	80	FCH
	Domain	422 – 611	190	Rho-GAP
	Coiled coil	830 – 859	30	Potential
	Coiled coil	1189 – 1219	31	Potential
	Compositional bias	294 – 300	7	Poly-Pro
	Compositional bias	319 – 324	6	Poly-Ser
	Compositional bias	621 – 629	9	Poly-Ser
	Compositional bias	665 – 675	11	Poly-Gln
	Compositional bias	734 – 749	16	Poly-Asn
	Compositional bias	1086 – 1096	11	Poly-Asn
	Compositional bias	1120 – 1132	13	Poly-Asn
	Compositional bias	1144 – 1158	15	Poly-Thr
	Compositional bias	1322 – 1336	15	Poly-Asn

Sequences

	Sequence		Length	Mass (Da)
	Q55DK5 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 73393E0FD5FD8AF4	FASTA	1,337	150,248
	10 20 30 40 50 60 MTVDNSQIPN LNQPESFADL WDGYENVSKA TEKGVLLLKD ITKFFKKRIQ SEDEYSKTLS 70 80 90 100 110 120 KLVLKFEPMA PDGYIGPRLK NTWDQIKLET LTHSNHHENI CNNISTHIIE PIEGLIVDLE 130 140 150 160 170 180 QKMKSIHVDA EKSFIHYQES VAKLKKAKQN YDRLCKDSFE ITGVSKGETQ KVQKRAIKAA 190 200 210 220 230 240 QDVIKADKDY RAQINETNTS QKQFLTELIP KIMNDLQRLE MVRIHMVKSY FHRYFKSMES 250 260 270 280 290 300 TPQKFNTETE NLLNLINSIN NEDEIQDFVR KSKTTHKYPK PFEYEPYLDR FTTPPPPPPP 310 320 330 340 350 360 QISSPQLLSP RGDISIQHSS SSNSLPIFPT QHQLLSNKEK ADNNITNSLS LSSDSLQTNL 370 380 390 400 410 420 NNGNNGGNGN NGSNTPTKEK KPSSWGLKRF STSVSSTSDR KLLNKQIQSI GLAHNGGNIF 430 440 450 460 470 480 NCKIEDIMVA QKKKYPYLEI PFIVVFLKHK LVALDVFKTQ GIFRVPGNVV DINSLKKRFD 490 500 510 520 530 540 EGNYEVAPTE NVYTIASLLK LWLREITEPL FPLTVYDQCI ENSNKREKIF EIISSLPILN 550 560 570 580 590 600 QKTISYIIEL LQECGKSVNV EHNKMNFPNL AMVFSPCFLR CSHTDPNILL GNIFKEKEFV 610 620 630 640 650 660 QNIIEHFKPL QVNDSLEGPP SSSSSSSTSI NQSSIESPPT SPLRSSTNSN ANKSSTPIKP 670 680 690 700 710 720 SSPSQQQQQQ QQQQQSSTPS TPLSSTTNTN VKHNILSPIV EQQSPNNGKQ PIALTQTYSH 730 740 750 760 770 780 LNIGSVPLII NTVNNNNQNQ NQNQNQNQNQ DQNQNQSKQP IQSSNQTQQQ VSAPATPHTV 790 800 810 820 830 840 AVNPKINQIK AETITTPQKS IGDGNGLIGQ SPSAHLMSPS EVKRRSNAIY LEDQERCKQR 850 860 870 880 890 900 IDELHTQVNE LYSDITTIET TTYFAMQSSL LITKLTKSLE SFLTIDMWNL TLQDIKEAIE 910 920 930 940 950 960 KNKFVSPPPI NFKIPSKMPK LSPIELVQNE QSSELLRSWL SNVTLTVNRI NEYLCYLGGV 970 980 990 1000 1010 1020 VIRIHSPDTL FAISNLFTDY DLSPQQNPKF VSLTLEQSTI FIQKTLALLE PLNPFTSDEL 1030 1040 1050 1060 1070 1080 NINVKVQDTI TPASFSPALI SDPDCNSPPT ISNTTNRLLN TSGSTDFSTT PLSSSPSTSS 1090 1100 1110 1120 1130 1140 TSLSTNNNNN NNGNRNLDIN NSPTIVITNS SLNKSALSTN NNNNNNNNNN NNPTTKLSSS 1150 1160 1170 1180 1190 1200 TSSTSTTTTT NTNTNTTTNE KIAHAIISPS SSTTSFKFDD DEDEDEDLLE INNKLHSQLT 1210 1220 1230 1240 1250 1260 EELKKKQQQY KQLIFDIIDM IKDKKKLMNS LDLTNSDSQL DIKSIAKLSL SLKRALDNFT 1270 1280 1290 1300 1310 1320 SESGFDIEDE QDPIINKEND SFVNLKIMTS HFISRIVLIL TTILSISNEF NSTLYQLLLP 1330 FNENNNNNNE NNNINNQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"Dictyostelium MEGAPs: F-BAR domain proteins that regulate motility and membrane tubulation in contractile vacuoles." Heath R.J., Insall R.H. J. Cell Sci. 121:1054-1064(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000005 Genomic DNA. Translation: EAL72162.1.
RefSeq	XP_646126.1. XM_641034.1.
3D structure databases
ProteinModelPortal	Q55DK5.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDBDRAFT_0190412.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0233874; DDB0233874; DDB_G0269624.
GeneID	8617075.
KEGG	ddi:DDB_G0269624.
Organism-specific databases
dictyBase	DDB_G0269624. mgp3.
Phylogenomic databases
eggNOG	NOG137357.
InParanoid	Q55DK5.
OMA	WLREITE.
Family and domain databases
Gene3D	1.10.555.10. 1 hit.
InterPro	IPR001060. FCH_dom. IPR008936. Rho_GTPase_activation_prot. IPR000198. RhoGAP_dom. [Graphical view]
Pfam	PF00611. FCH. 1 hit. PF00620. RhoGAP. 1 hit. [Graphical view]
SMART	SM00324. RhoGAP. 1 hit. [Graphical view]
SUPFAM	SSF48350. Rho_GAP. 1 hit.
PROSITE	PS50133. FCH. 1 hit. PS50238. RHOGAP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MGP3_DICDI

Accession

Primary (citable) accession number: Q55DK5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	May 24, 2005
Last modified:	May 1, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)