Reviewed, UniProtKB/Swiss-Prot Q55CY3 (HUTI_DICDI)

Names and origin

Protein names

Recommended name:
    Probable imidazolonepropionase
    EC=3.5.2.7
Alternative name(s):
    Amidohydrolase domain-containing protein 1 homolog

Gene names

Name:	amdhd1
ORF Names:	DDB_G0269854

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+.
Cofactor	Binds 1 zinc or iron ion per subunit By similarity.
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3.
Sequence similarities	Belongs to the hutI family.

Ontologies

Keywords
Biological process	Histidine metabolism
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine catabolic process to glutamate and formamide Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	imidazolonepropionase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 426	426	Probable imidazolonepropionase		PRO_0000328424
Sites
	Metal binding	86	1	Zinc or iron By similarity
	Metal binding	88	1	Zinc or iron By similarity
	Metal binding	260	1	Zinc or iron By similarity
	Metal binding	334	1	Zinc or iron By similarity
	Binding site	95	1	Substrate By similarity
	Binding site	108	1	Substrate By similarity
	Binding site	158	1	Substrate By similarity
	Binding site	192	1	Substrate By similarity
	Binding site	263	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q55CY3-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 0130BE38EAE04726	FASTA	426	46,887
	10 20 30 40 50 60 MGFDFKLKIS NASQLVVISK GKPFLIGKEM SNIEIIENGT MIIDENGIIF DIGTFKEMEE 70 80 90 100 110 120 KYKDKSFEKV LDCTGKSVLP GFVDGHTHPV FSGDRVHEFA MKLAGATYLD VHKAGGGIQF 130 140 150 160 170 180 TISHTKNSTE DELYQLLIPR LNRMLKNGTT LIEAKSGYGL ETETEMKMLK VLDRASKQFQ 190 200 210 220 230 240 GVEIVSTYLG GHAIPKGMNA SEATDDIINK QIPELKRLKD AGEISPANID VFLEKGFFEY 250 260 270 280 290 300 EDTKRILQAG KDIGLECNFH GDELSYMKSG ELAGELGCRA ISHLEKVSED GMKAMAATPT 310 320 330 340 350 360 FAVLLPTTAY ILRLECPPAR RMIELGVPVA LGSDFNPNAH CLSMPFVMNL ACVLMKMNMN 370 380 390 400 410 420 ESLVAATLNA AASINKSSTH GSLEVGKFAD FVILSADKWE HIIYEMVDPP ISHVFKKGNL VFSNNN

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000005 Genomic DNA. Translation: EAL72277.1.
RefSeq	XP_646348.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q55CY3.
Genome annotation databases
GeneID	3397307.
GenomeReviews	Gene locus amdhd1 in contig CM000150_GR.
KEGG	ddi:DDBDRAFT_0190619.
Organism-specific databases
dictyBase	DDB_G0269854. amdhd1.
Phylogenomic databases
OMA	MNMACTL.
Enzyme and pathway databases
BRENDA	3.5.2.7. 424.
Family and domain databases
InterPro	IPR006680. Amidohydro_1. IPR005920. HutI. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
ProDom	PD001248. Amidohydro_like. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01224. hutI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HUTI_DICDI

Accession

Primary (citable) accession number: Q55CY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	November 3, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)