Q55CT5 (SEC31_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein transport protein SEC31

Gene names

Name:	sec31
ORF Names:	DDB_G0270992

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1355 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules By similarity.
Subunit structure	The COPII coat is composed of at least 5 proteins: the sec23/24 complex, the sec13/31 complex, and the protein sar1A or sar1B. sec13 and sec31 make a 2:2 tetramer that forms the edge element of the COPII outer coat. The tetramer self-assembles in multiple copies to form the complete polyhedral cage. Interacts (via WD 8) with sec13 By similarity.
Subcellular location	Cytoplasmic vesicle › COPII-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side By similarity.
Sequence similarities	Belongs to the WD repeat SEC31 family. Contains 8 WD repeats.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Cytoplasmic vesicle Endoplasmic reticulum Membrane
Domain	Repeat WD repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ER to Golgi vesicle-mediated transport Inferred from sequence or structural similarity. Source: dictyBase RNA splicing Inferred from electronic annotation. Source: InterPro intracellular protein transport Inferred from sequence or structural similarity. Source: dictyBase
Cellular_component	COPII vesicle coat Inferred from sequence or structural similarity. Source: dictyBase endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell spliceosomal complex Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1355	1355	Protein transport protein SEC31		PRO_0000328076
Regions
	Repeat	5 – 48	44	WD 1
	Repeat	65 – 109	45	WD 2
	Repeat	127 – 167	41	WD 3
	Repeat	175 – 215	41	WD 4
	Repeat	220 – 263	44	WD 5
	Repeat	267 – 307	41	WD 6
	Repeat	334 – 374	41	WD 7
	Repeat	412 – 463	52	WD 8; interaction with sec13 By similarity
	Compositional bias	437 – 443	7	Poly-Ser
	Compositional bias	449 – 457	9	Poly-Gln
	Compositional bias	570 – 574	5	Poly-Thr
	Compositional bias	612 – 617	6	Poly-Ser
	Compositional bias	622 – 626	5	Poly-Thr
	Compositional bias	774 – 782	9	Poly-Gln
	Compositional bias	902 – 909	8	Poly-Gln
	Compositional bias	943 – 953	11	Poly-Gln
	Compositional bias	969 – 980	12	Poly-Gln
	Compositional bias	984 – 1188	205	Met/Pro-rich

Sequences

	Sequence		Length	Mass (Da)
	Q55CT5 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 885303FDEEB8959F	FASTA	1,355	150,127
	10 20 30 40 50 60 MSKLKEISRQ STTSWSPIAQ YPDYMAVGTV TGTIGADFDT SSKLEIYSLD ITNESKQMTL 70 80 90 100 110 120 KGSTSSSTRF NKVVWGQASS NFQNGIIAGA MDNGTINLWD PTKILASDAT DGGGSDDQSS 130 140 150 160 170 180 LIGVGQRHSG PVQSIDFNVQ NPNLLASGGS DSEVFIWDLS DPTQPSALNP GSKSQQSSDI 190 200 210 220 230 240 TCVAWNKKVA HILGSASYNG YIVIWDLKSK KTLMTINDRN RKCKYRSIVW HPSEATQIVA 250 260 270 280 290 300 ASEDDDHPVI QAWDLRNTTS PVKSMEGHKK GVWGLSWCPS DNALLLSTGK DNKTFCWNFD 310 320 330 340 350 360 RQEILCEIND NNSRNNINNN SNNSNSDNNN GNTDPNAWNF EVQWSPRVPA LLSTSSYVGK 370 380 390 400 410 420 VNVYSLQDVN EKSTSGSSAG QLNALGIQEQ TSQITPTIKH TPNWLLRPCG AAFGFGGKIA 430 440 450 460 470 480 VFGRNKKVTA AANGATSPSS SSSPASSVQQ SQLQQQQRVI HISHVTTEVD IVKSSEQLEN 490 500 510 520 530 540 VIHTGQYEQY CQEKIDQSTS DEEKSIWGFL KVKFAKDDRL KILDYLGYDI ETIKKELKQF 550 560 570 580 590 600 LGTLPELPIE SGFNELPIEN VDDQSKPEPT TTTTNETVHL EPVVDENNNV VDADSFFDTA 610 620 630 640 650 660 ASDANKEQQD DSSSSPSTKS PTTTTTPIEF PGDEKEQMIT KALLVGDHNS AVECCLRLGR 670 680 690 700 710 720 YSDALILAHA AGQELWKKTQ EAYFEIVRSP FGRVVSCIVK RDFQLLVKSA DLKDWKASLA 730 740 750 760 770 780 ILCTYAPPTD FKILSGILGD RLDKEASDLK SAILCYICAG DIDKTVDIWS RVSQQHQQQQ 790 800 810 820 830 840 RQSLTSSGNS ITVLEQESNK DLQNLIEKVS IFRSACNGNS NNNTLNQVLS MKYAKYAEIL 850 860 870 880 890 900 ASQGNLSASL RYLAPITNSQ CKQEYGVLFD RVYRATSNHQ GIPQPPFPFQ LVDVYSSNQP 910 920 930 940 950 960 IQQQQQQQQN KAQQVGHQHQ HQHQHQNQHQ HQHQHQHQHQ PPQQQQQQQQ QQQMGRQNTF 970 980 990 1000 1010 1020 NQPPQPMGQH QHQQQQQQQQ PPIMMNQSPM QNNNNNRIPM MNQPPMMNQP PMMNQPPMMN 1030 1040 1050 1060 1070 1080 QPPQMMNQPP QMMNQPPPQM MNQPPPQMMN NNQPPIMMRP MQPSGPSPMN PPPTMNNTQP 1090 1100 1110 1120 1130 1140 PPMMNTMGGP SSVNNSQPPI IPMNGNPLPM NPMSPMMSDK SNQPPMNPMM NPMNPMNPMM 1150 1160 1170 1180 1190 1200 NPIQPTAPPP MNPMIPQGGS PMINHPPPPM NPMINNGPVS TPPMNPMMNQ VVPQNISPPQ 1210 1220 1230 1240 1250 1260 MPTRSPVLEN KSSSPSSESF TSPAPKPNVH NKTPSIITGQ LGVTTPSEGP SNEDTERFVE 1270 1280 1290 1300 1310 1320 KLQRSIQELN GRTDSKVWED ANKRCQGLIN KVSKKDISAE AFKALDAILA SIVEKDFKKA 1330 1340 1350 SDTYIQITST PLWGEVGSQS MVGLKRLIDL GLKSH

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000005 Genomic DNA. Translation: EAL72846.1.
RefSeq	XP_646396.1. XM_641304.1.
3D structure databases
ProteinModelPortal	Q55CT5.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDBDRAFT_0216723.
Proteomic databases
PRIDE	Q55CT5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0235185; DDB0235185; DDB_G0270992.
GeneID	8617351.
KEGG	ddi:DDB_G0270992.
Organism-specific databases
dictyBase	DDB_G0270992. sec31.
Phylogenomic databases
eggNOG	NOG248389.
KO	K14005.
OMA	MMNQPPM.
Family and domain databases
Gene3D	2.130.10.10. 1 hit.
InterPro	IPR004098. Prp18. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view]
Pfam	PF02840. Prp18. 1 hit. PF00400. WD40. 3 hits. [Graphical view]
SMART	SM00320. WD40. 5 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. 2 hits. PS50082. WD_REPEATS_2. 3 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SEC31_DICDI

Accession

Primary (citable) accession number: Q55CT5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)