Q55CT0 (TPP1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tripeptidyl-peptidase 1
Short name=TPP-1
EC=3.4.14.9

Alternative name(s):
Tripeptidyl aminopeptidase
Tripeptidyl-peptidase I
Short name=TPP-I

Gene names

Name:	tpp1
ORF Names:	DDB_G0269914

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	600 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Serine protease with tripeptidyl-peptidase I activity By similarity.
Catalytic activity	Release of an N-terminal tripeptide from a polypeptide, but also has endopeptidase activity.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Secreted Potential.
Post-translational modification	Activated by autocatalytic proteolytical processing upon acidification. N-glycosylation is required for processing and activity By similarity.
Sequence similarities	Belongs to the peptidase S53 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Serine protease
PTM	Autocatalytic cleavage Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from sequence or structural similarity. Source: dictyBase
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro tripeptidyl-peptidase activity Inferred from sequence or structural similarity. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Propeptide	23 – 220	198	Removed in mature form By similarity		PRO_0000327570
	Chain	221 – 600	380	Tripeptidyl-peptidase 1		PRO_0000327571
Sites
	Active site	318	1	Charge relay system By similarity
	Active site	322	1	Charge relay system By similarity
	Active site	514	1	Charge relay system By similarity
	Metal binding	559	1	Calcium By similarity
	Metal binding	560	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	579	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	581	1	Calcium By similarity
Amino acid modifications
	Glycosylation	91	1	N-linked (GlcNAc...) Potential
	Glycosylation	259	1	N-linked (GlcNAc...) Potential
	Glycosylation	266	1	N-linked (GlcNAc...) Potential
	Glycosylation	475	1	N-linked (GlcNAc...) Potential
	Glycosylation	483	1	N-linked (GlcNAc...) Potential
	Disulfide bond	411 ↔ 570		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q55CT0 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: D9E97C1279BC9C18	FASTA	600	67,296
	10 20 30 40 50 60 MNIKFNLIII ILFILFISNV NCKKIKNKKH LTPQRLRRFV EHSKPISLNK KVWKITEIEN 70 80 90 100 110 120 IFSAQIELTF GIRQRNIVEL EDFVWRVSDP NDSLYGSYKT FEEIKEWVKP LDESIDAVKN 130 140 150 160 170 180 WLIENDINEF TVTKSGDFIR TIVSIDKAEE LLSVRYNKMV HKLSKQSFFR SLDPYTIPRE 190 200 210 220 230 240 LYDHIDFIGG VNHLPLLSPR PKESSGSAGG GGGGKVNGIG YELESLRNNK QIKSFNDKKV 250 260 270 280 290 300 AARNGDPYLS PDLIRKEMNV SQTSTNSTHL GNSQAIAQFL KEYFSPSDLK IFQYRFGLEP 310 320 330 340 350 360 SQVDNIIGPN QNLNPGIETA LDIQYIMAMA PDVPTWIVST GGLHEGQEPF LDWLVDLSSN 370 380 390 400 410 420 PKLPLVHSIS YGDDESSIGL AYTDRVDTEF KKYAAMGRTI VFSSGDFGVG CNDDCDSFSP 430 440 450 460 470 480 GWPASSRFVL AVGGVIKKKD GSIIGDEISG GGFSNYFSRP WYQVDECSSY IEWLNGSLSS 490 500 510 520 530 540 FYNQSGRGFP DISSFSENVV ILYKDKLMPI GGTSASAPII AGLLSLINDQ RLQKNQSPIG 550 560 570 580 590 600 LFNPLLYKIA RDHPNSFLDI DFGENNYKCC TNGFKSKSGW DPVTGLGLPN FDELVKYCLE

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000005 Genomic DNA. Translation: EAL72307.1.
RefSeq	XP_646401.1. XM_641309.1.
3D structure databases
ProteinModelPortal	Q55CT0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0234303; DDB0234303; DDB_G0269914.
GeneID	8617356.
KEGG	ddi:DDB_G0269914.
Organism-specific databases
dictyBase	DDB_G0269914. tpp1.
Phylogenomic databases
eggNOG	COG4934.
KO	K01279.
OMA	YIMAMAP.
ProtClustDB	CLSZ2429688.
Family and domain databases
Gene3D	3.40.50.200. 1 hit.
InterPro	IPR015366. Peptidase_S53_propep. IPR000209. Peptidase_S8/S53_dom. IPR023828. Peptidase_S8_Ser-AS. IPR009020. Prot_inh_propept. [Graphical view]
Pfam	PF00082. Peptidase_S8. 1 hit. PF09286. Pro-kuma_activ. 1 hit. [Graphical view]
SMART	SM00944. Pro-kuma_activ. 1 hit. [Graphical view]
SUPFAM	SSF52743. Pept_S8_S53. 1 hit. SSF54897. Prot_inh_propept. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPP1_DICDI

Accession

Primary (citable) accession number: Q55CT0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)