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Protein

Tripeptidyl-peptidase 1

Gene

tpp1

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Serine protease with tripeptidyl-peptidase I activity.By similarity

Catalytic activityi

Release of an N-terminal tripeptide from a polypeptide, but also has endopeptidase activity.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei318 – 3181Charge relay systemBy similarity
Active sitei322 – 3221Charge relay systemBy similarity
Active sitei514 – 5141Charge relay systemBy similarity
Metal bindingi559 – 5591CalciumBy similarity
Metal bindingi560 – 5601Calcium; via carbonyl oxygenBy similarity
Metal bindingi579 – 5791Calcium; via carbonyl oxygenBy similarity
Metal bindingi581 – 5811CalciumBy similarity

GO - Molecular functioni

  1. endopeptidase activity Source: GO_Central
  2. metal ion binding Source: UniProtKB-KW
  3. serine-type endopeptidase activity Source: InterPro
  4. tripeptidyl-peptidase activity Source: dictyBase

GO - Biological processi

  1. proteolysis Source: dictyBase
  2. response to chloroquine Source: dictyBase
  3. sorocarp development Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

MEROPSiS53.A02.

Names & Taxonomyi

Protein namesi
Recommended name:
Tripeptidyl-peptidase 1 (EC:3.4.14.9)
Short name:
TPP-1
Alternative name(s):
Tripeptidyl aminopeptidase
Tripeptidyl-peptidase I
Short name:
TPP-I
Gene namesi
Name:tpp1
ORF Names:DDB_G0269914
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195 Componentsi: Chromosome 1, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0269914. tpp1.

Subcellular locationi

  1. Secreted Curated

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
  2. lysosome Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Propeptidei23 – 220198Removed in mature formBy similarityPRO_0000327570Add
BLAST
Chaini221 – 600380Tripeptidyl-peptidase 1PRO_0000327571Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi91 – 911N-linked (GlcNAc...)Sequence Analysis
Glycosylationi259 – 2591N-linked (GlcNAc...)Sequence Analysis
Glycosylationi266 – 2661N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi411 ↔ 570By similarity
Glycosylationi475 – 4751N-linked (GlcNAc...)Sequence Analysis
Glycosylationi483 – 4831N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Activated by autocatalytic proteolytical processing upon acidification. N-glycosylation is required for processing and activity (By similarity).By similarity

Keywords - PTMi

Autocatalytic cleavage, Disulfide bond, Glycoprotein, Zymogen

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi44689.DDBDRAFT_0190668.

Structurei

3D structure databases

ProteinModelPortaliQ55CT0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini248 – 600353Peptidase S53Add
BLAST

Sequence similaritiesi

Contains 1 peptidase S53 domain.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG4934.
InParanoidiQ55CT0.
KOiK01279.
OMAiYIMAMAP.
PhylomeDBiQ55CT0.

Family and domain databases

Gene3Di3.40.50.200. 1 hit.
InterProiIPR015366. Peptidase_S53_propep.
IPR000209. Peptidase_S8/S53_dom.
IPR023828. Peptidase_S8_Ser-AS.
IPR009020. Prot_inh_propept.
IPR030400. Sedolisin_dom.
[Graphical view]
PfamiPF00082. Peptidase_S8. 1 hit.
PF09286. Pro-kuma_activ. 1 hit.
[Graphical view]
SMARTiSM00944. Pro-kuma_activ. 1 hit.
[Graphical view]
SUPFAMiSSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS51695. SEDOLISIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q55CT0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIKFNLIII ILFILFISNV NCKKIKNKKH LTPQRLRRFV EHSKPISLNK
60 70 80 90 100
KVWKITEIEN IFSAQIELTF GIRQRNIVEL EDFVWRVSDP NDSLYGSYKT
110 120 130 140 150
FEEIKEWVKP LDESIDAVKN WLIENDINEF TVTKSGDFIR TIVSIDKAEE
160 170 180 190 200
LLSVRYNKMV HKLSKQSFFR SLDPYTIPRE LYDHIDFIGG VNHLPLLSPR
210 220 230 240 250
PKESSGSAGG GGGGKVNGIG YELESLRNNK QIKSFNDKKV AARNGDPYLS
260 270 280 290 300
PDLIRKEMNV SQTSTNSTHL GNSQAIAQFL KEYFSPSDLK IFQYRFGLEP
310 320 330 340 350
SQVDNIIGPN QNLNPGIETA LDIQYIMAMA PDVPTWIVST GGLHEGQEPF
360 370 380 390 400
LDWLVDLSSN PKLPLVHSIS YGDDESSIGL AYTDRVDTEF KKYAAMGRTI
410 420 430 440 450
VFSSGDFGVG CNDDCDSFSP GWPASSRFVL AVGGVIKKKD GSIIGDEISG
460 470 480 490 500
GGFSNYFSRP WYQVDECSSY IEWLNGSLSS FYNQSGRGFP DISSFSENVV
510 520 530 540 550
ILYKDKLMPI GGTSASAPII AGLLSLINDQ RLQKNQSPIG LFNPLLYKIA
560 570 580 590 600
RDHPNSFLDI DFGENNYKCC TNGFKSKSGW DPVTGLGLPN FDELVKYCLE
Length:600
Mass (Da):67,296
Last modified:May 24, 2005 - v1
Checksum:iD9E97C1279BC9C18
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000005 Genomic DNA. Translation: EAL72307.1.
RefSeqiXP_646401.1. XM_641309.1.

Genome annotation databases

EnsemblProtistsiDDB0234303; DDB0234303; DDB_G0269914.
GeneIDi8617356.
KEGGiddi:DDB_G0269914.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000005 Genomic DNA. Translation: EAL72307.1.
RefSeqiXP_646401.1. XM_641309.1.

3D structure databases

ProteinModelPortaliQ55CT0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDBDRAFT_0190668.

Protein family/group databases

MEROPSiS53.A02.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0234303; DDB0234303; DDB_G0269914.
GeneIDi8617356.
KEGGiddi:DDB_G0269914.

Organism-specific databases

dictyBaseiDDB_G0269914. tpp1.

Phylogenomic databases

eggNOGiCOG4934.
InParanoidiQ55CT0.
KOiK01279.
OMAiYIMAMAP.
PhylomeDBiQ55CT0.

Miscellaneous databases

PROiQ55CT0.

Family and domain databases

Gene3Di3.40.50.200. 1 hit.
InterProiIPR015366. Peptidase_S53_propep.
IPR000209. Peptidase_S8/S53_dom.
IPR023828. Peptidase_S8_Ser-AS.
IPR009020. Prot_inh_propept.
IPR030400. Sedolisin_dom.
[Graphical view]
PfamiPF00082. Peptidase_S8. 1 hit.
PF09286. Pro-kuma_activ. 1 hit.
[Graphical view]
SMARTiSM00944. Pro-kuma_activ. 1 hit.
[Graphical view]
SUPFAMiSSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS51695. SEDOLISIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiTPP1_DICDI
AccessioniPrimary (citable) accession number: Q55CT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: April 29, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.