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Q55CS7

- MPL1_DICDI

UniProt

Q55CS7 - MPL1_DICDI

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Protein

MAP kinase phosphatase with leucine-rich repeats protein 1

Gene

mpl1

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Probable phosphatase with dual specificity toward Ser/Thr and Tyr-containing proteins (By similarity). Dephosphorylates pNPP, in vitro. Essential for proper regulation of erkB (erk2) and optimal motility during development.By similarity1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei778 – 7781Phosphocysteine intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. phosphatase activity Source: dictyBase
  2. protein tyrosine/serine/threonine phosphatase activity Source: dictyBase
  3. protein tyrosine phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. chemotaxis to cAMP Source: dictyBase
  2. dephosphorylation Source: dictyBase
  3. negative regulation of MAP kinase activity Source: dictyBase
  4. protein dephosphorylation Source: dictyBase
  5. regulation of cellular component movement Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Chemotaxis

Names & Taxonomyi

Protein namesi
Recommended name:
MAP kinase phosphatase with leucine-rich repeats protein 1 (EC:3.1.3.16, EC:3.1.3.48)
Gene namesi
Name:mpl1
ORF Names:DDB_G0269918
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 1, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0269918. mpl1.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: dictyBase
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Cells result in higher prestimulus and persistent stimulation erkB phosphorylation upon cAMP stimulation, display strong defects in motility, and more rapid cAMP production. Cells fail to display aggregates, fruiting bodies, fail to form territorial streams and are defective in cell migration (absence of random and directional migration) and in chemotaxis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 834834MAP kinase phosphatase with leucine-rich repeats protein 1PRO_0000332956Add
BLAST

Proteomic databases

PRIDEiQ55CS7.

Expressioni

Developmental stagei

Expressed (at protein levels). Up-regulated upon starvation, reaching a maximum around 10 hours. Expression subsequently declined but is present throughout development.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi44689.DDBDRAFT_0190671.

Structurei

3D structure databases

ProteinModelPortaliQ55CS7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati160 – 18122LRR 1Add
BLAST
Repeati183 – 20422LRR 2Add
BLAST
Repeati206 – 22621LRR 3Add
BLAST
Repeati229 – 25123LRR 4Add
BLAST
Repeati252 – 27322LRR 5Add
BLAST
Repeati274 – 29219LRR 6Add
BLAST
Repeati298 – 31922LRR 7Add
BLAST
Repeati321 – 34222LRR 8Add
BLAST
Repeati345 – 36622LRR 9Add
BLAST
Repeati368 – 38922LRR 10Add
BLAST
Domaini695 – 833139Tyrosine-protein phosphataseAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili555 – 61561Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi12 – 7059Thr-richAdd
BLAST
Compositional biasi428 – 4325Poly-Thr
Compositional biasi481 – 49010Poly-Thr
Compositional biasi518 – 5214Poly-Thr
Compositional biasi561 – 5677Poly-Asn

Sequence similaritiesi

Contains 10 LRR (leucine-rich) repeats.Curated

Keywords - Domaini

Coiled coil, Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiCOG4886.
InParanoidiQ55CS7.
OMAiKGSGTYY.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR001611. Leu-rich_rpt.
IPR025875. Leu-rich_rpt_4.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
PF12799. LRR_4. 1 hit.
PF13504. LRR_7. 1 hit.
PF13855. LRR_8. 1 hit.
[Graphical view]
SMARTiSM00195. DSPc. 1 hit.
SM00369. LRR_TYP. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS51450. LRR. 9 hits.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q55CS7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIFKKLFSKG STSPTTRPRG ATFSGTFPTD VLSDDGSGTN TNGLSNSTTN
60 70 80 90 100
PSSIHSTPTT PTTTASTNLT NSNKLSTLAP ITNGNRSLRG SKDGSGTTKE
110 120 130 140 150
SKKKVLTLNE KQKLLLKSME YIKGSGTYYG NYMEFYEIPI QIYVGTEPSE
160 170 180 190 200
TYPSLSYNTE LRSLILDFNK ITEIPEQIGL LPNLKHLSLA ANQLSQVPEF
210 220 230 240 250
LSQLKSLESL ELGINQFTSF PLNICKIKSL TLLRLETNNI KSLPDDFINL
260 270 280 290 300
ENLKDLSLLD NQLKEIPDSL PNNIEKLNLG CNDIINSYSK SLIRISHSLT
310 320 330 340 350
TLNLSENKIE VLDESLSCLV NVKTLILDCN MIKVIPGSVL GSWKSLVTLN
360 370 380 390 400
LPHNFISDLP AEIVTLDNLR IIDLRGNNFE FCKNYPSSES SSILFKIEEF
410 420 430 440 450
IKDKEKLKSL ILKENLEILS KLKDDNSTTT TTNINSNLDV PIIITTNIET
460 470 480 490 500
IPTTSTTATT TETTNDITFK ISDITEIIEK TDTTTTTTTT NQTDNVKLEE
510 520 530 540 550
KVYEKQENDE NNSVTLETTT TISIASDNTD EASIQIPQKE DGDKENLEND
560 570 580 590 600
DKLLQESFSE NNNNNNNEKQ QEQQENPLKE SQGKIQQLEE ELEKLEQKQL
610 620 630 640 650
ELKDKIRLEK IKYQEIQQQS PRLSQQENNQ EAIVVNTQPS SPPPTIIVNE
660 670 680 690 700
QKSEKLENEK PTKREQPMVV VTKNNNKAEV EMTAPNQLIF WQSIVPDLII
710 720 730 740 750
DKLYLGCREC AMNKSWLKDN NVTHILTVAN FKPLYPDLFK YLIINIDDVD
760 770 780 790 800
EANIYQYFKE MNTFIDEGRE KGGVLIHCRA GVSRSATATI AYIMMKNSVK
810 820 830
FQEAFDITIK GRSRIYPNRG FLNQLKKFEK DLSK
Length:834
Mass (Da):94,023
Last modified:May 24, 2005 - v1
Checksum:i7DEDB07F407771FE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000005 Genomic DNA. Translation: EAL72309.1.
RefSeqiXP_646404.1. XM_641312.1.

Genome annotation databases

EnsemblProtistsiDDB0238871; DDB0238871; DDB_G0269918.
GeneIDi8617360.
KEGGiddi:DDB_G0269918.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000005 Genomic DNA. Translation: EAL72309.1 .
RefSeqi XP_646404.1. XM_641312.1.

3D structure databases

ProteinModelPortali Q55CS7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDBDRAFT_0190671.

Proteomic databases

PRIDEi Q55CS7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0238871 ; DDB0238871 ; DDB_G0269918 .
GeneIDi 8617360.
KEGGi ddi:DDB_G0269918.

Organism-specific databases

dictyBasei DDB_G0269918. mpl1.

Phylogenomic databases

eggNOGi COG4886.
InParanoidi Q55CS7.
OMAi KGSGTYY.

Family and domain databases

Gene3Di 3.90.190.10. 1 hit.
InterProi IPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR001611. Leu-rich_rpt.
IPR025875. Leu-rich_rpt_4.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view ]
PANTHERi PTHR10159. PTHR10159. 1 hit.
Pfami PF00782. DSPc. 1 hit.
PF12799. LRR_4. 1 hit.
PF13504. LRR_7. 1 hit.
PF13855. LRR_8. 1 hit.
[Graphical view ]
SMARTi SM00195. DSPc. 1 hit.
SM00369. LRR_TYP. 1 hit.
[Graphical view ]
SUPFAMi SSF52799. SSF52799. 1 hit.
PROSITEi PS51450. LRR. 9 hits.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "MPL1, a novel phosphatase with leucine-rich repeats, is essential for proper ERK2 phosphorylation and cell motility."
    Rodriguez M., Kim B., Lee N.-S., Veeranki S., Kim L.
    Eukaryot. Cell 7:958-966(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiMPL1_DICDI
AccessioniPrimary (citable) accession number: Q55CS7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 24, 2005
Last modified: October 29, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3