Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q55CS7

- MPL1_DICDI

UniProt

Q55CS7 - MPL1_DICDI

Protein

MAP kinase phosphatase with leucine-rich repeats protein 1

Gene

mpl1

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Probable phosphatase with dual specificity toward Ser/Thr and Tyr-containing proteins By similarity. Dephosphorylates pNPP, in vitro. Essential for proper regulation of erkB (erk2) and optimal motility during development.By similarity1 Publication

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei778 – 7781Phosphocysteine intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. phosphatase activity Source: dictyBase
    2. protein tyrosine/serine/threonine phosphatase activity Source: dictyBase
    3. protein tyrosine phosphatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. chemotaxis to cAMP Source: dictyBase
    2. dephosphorylation Source: dictyBase
    3. negative regulation of MAP kinase activity Source: dictyBase
    4. protein dephosphorylation Source: dictyBase
    5. regulation of cellular component movement Source: dictyBase

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Chemotaxis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    MAP kinase phosphatase with leucine-rich repeats protein 1 (EC:3.1.3.16, EC:3.1.3.48)
    Gene namesi
    Name:mpl1
    ORF Names:DDB_G0269918
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 1, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0269918. mpl1.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: dictyBase

    Pathology & Biotechi

    Disruption phenotypei

    Cells result in higher prestimulus and persistent stimulation erkB phosphorylation upon cAMP stimulation, display strong defects in motility, and more rapid cAMP production. Cells fail to display aggregates, fruiting bodies, fail to form territorial streams and are defective in cell migration (absence of random and directional migration) and in chemotaxis.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 834834MAP kinase phosphatase with leucine-rich repeats protein 1PRO_0000332956Add
    BLAST

    Proteomic databases

    PRIDEiQ55CS7.

    Expressioni

    Developmental stagei

    Expressed (at protein levels). Up-regulated upon starvation, reaching a maximum around 10 hours. Expression subsequently declined but is present throughout development.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDBDRAFT_0190671.

    Structurei

    3D structure databases

    ProteinModelPortaliQ55CS7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati160 – 18122LRR 1Add
    BLAST
    Repeati183 – 20422LRR 2Add
    BLAST
    Repeati206 – 22621LRR 3Add
    BLAST
    Repeati229 – 25123LRR 4Add
    BLAST
    Repeati252 – 27322LRR 5Add
    BLAST
    Repeati274 – 29219LRR 6Add
    BLAST
    Repeati298 – 31922LRR 7Add
    BLAST
    Repeati321 – 34222LRR 8Add
    BLAST
    Repeati345 – 36622LRR 9Add
    BLAST
    Repeati368 – 38922LRR 10Add
    BLAST
    Domaini695 – 833139Tyrosine-protein phosphataseAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili555 – 61561Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi12 – 7059Thr-richAdd
    BLAST
    Compositional biasi428 – 4325Poly-Thr
    Compositional biasi481 – 49010Poly-Thr
    Compositional biasi518 – 5214Poly-Thr
    Compositional biasi561 – 5677Poly-Asn

    Sequence similaritiesi

    Contains 10 LRR (leucine-rich) repeats.Curated

    Keywords - Domaini

    Coiled coil, Leucine-rich repeat, Repeat

    Phylogenomic databases

    eggNOGiCOG4886.
    OMAiKGSGTYY.

    Family and domain databases

    Gene3Di3.90.190.10. 1 hit.
    InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
    IPR020422. Dual-sp_phosphatase_subgr_cat.
    IPR024950. DUSP.
    IPR001611. Leu-rich_rpt.
    IPR025875. Leu-rich_rpt_4.
    IPR003591. Leu-rich_rpt_typical-subtyp.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    [Graphical view]
    PANTHERiPTHR10159. PTHR10159. 1 hit.
    PfamiPF00782. DSPc. 1 hit.
    PF12799. LRR_4. 1 hit.
    PF13504. LRR_7. 1 hit.
    PF13855. LRR_8. 1 hit.
    [Graphical view]
    SMARTiSM00195. DSPc. 1 hit.
    SM00369. LRR_TYP. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 1 hit.
    PROSITEiPS51450. LRR. 9 hits.
    PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q55CS7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIFKKLFSKG STSPTTRPRG ATFSGTFPTD VLSDDGSGTN TNGLSNSTTN    50
    PSSIHSTPTT PTTTASTNLT NSNKLSTLAP ITNGNRSLRG SKDGSGTTKE 100
    SKKKVLTLNE KQKLLLKSME YIKGSGTYYG NYMEFYEIPI QIYVGTEPSE 150
    TYPSLSYNTE LRSLILDFNK ITEIPEQIGL LPNLKHLSLA ANQLSQVPEF 200
    LSQLKSLESL ELGINQFTSF PLNICKIKSL TLLRLETNNI KSLPDDFINL 250
    ENLKDLSLLD NQLKEIPDSL PNNIEKLNLG CNDIINSYSK SLIRISHSLT 300
    TLNLSENKIE VLDESLSCLV NVKTLILDCN MIKVIPGSVL GSWKSLVTLN 350
    LPHNFISDLP AEIVTLDNLR IIDLRGNNFE FCKNYPSSES SSILFKIEEF 400
    IKDKEKLKSL ILKENLEILS KLKDDNSTTT TTNINSNLDV PIIITTNIET 450
    IPTTSTTATT TETTNDITFK ISDITEIIEK TDTTTTTTTT NQTDNVKLEE 500
    KVYEKQENDE NNSVTLETTT TISIASDNTD EASIQIPQKE DGDKENLEND 550
    DKLLQESFSE NNNNNNNEKQ QEQQENPLKE SQGKIQQLEE ELEKLEQKQL 600
    ELKDKIRLEK IKYQEIQQQS PRLSQQENNQ EAIVVNTQPS SPPPTIIVNE 650
    QKSEKLENEK PTKREQPMVV VTKNNNKAEV EMTAPNQLIF WQSIVPDLII 700
    DKLYLGCREC AMNKSWLKDN NVTHILTVAN FKPLYPDLFK YLIINIDDVD 750
    EANIYQYFKE MNTFIDEGRE KGGVLIHCRA GVSRSATATI AYIMMKNSVK 800
    FQEAFDITIK GRSRIYPNRG FLNQLKKFEK DLSK 834
    Length:834
    Mass (Da):94,023
    Last modified:May 24, 2005 - v1
    Checksum:i7DEDB07F407771FE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000005 Genomic DNA. Translation: EAL72309.1.
    RefSeqiXP_646404.1. XM_641312.1.

    Genome annotation databases

    EnsemblProtistsiDDB0238871; DDB0238871; DDB_G0269918.
    GeneIDi8617360.
    KEGGiddi:DDB_G0269918.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000005 Genomic DNA. Translation: EAL72309.1 .
    RefSeqi XP_646404.1. XM_641312.1.

    3D structure databases

    ProteinModelPortali Q55CS7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDBDRAFT_0190671.

    Proteomic databases

    PRIDEi Q55CS7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0238871 ; DDB0238871 ; DDB_G0269918 .
    GeneIDi 8617360.
    KEGGi ddi:DDB_G0269918.

    Organism-specific databases

    dictyBasei DDB_G0269918. mpl1.

    Phylogenomic databases

    eggNOGi COG4886.
    OMAi KGSGTYY.

    Family and domain databases

    Gene3Di 3.90.190.10. 1 hit.
    InterProi IPR000340. Dual-sp_phosphatase_cat-dom.
    IPR020422. Dual-sp_phosphatase_subgr_cat.
    IPR024950. DUSP.
    IPR001611. Leu-rich_rpt.
    IPR025875. Leu-rich_rpt_4.
    IPR003591. Leu-rich_rpt_typical-subtyp.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    [Graphical view ]
    PANTHERi PTHR10159. PTHR10159. 1 hit.
    Pfami PF00782. DSPc. 1 hit.
    PF12799. LRR_4. 1 hit.
    PF13504. LRR_7. 1 hit.
    PF13855. LRR_8. 1 hit.
    [Graphical view ]
    SMARTi SM00195. DSPc. 1 hit.
    SM00369. LRR_TYP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52799. SSF52799. 1 hit.
    PROSITEi PS51450. LRR. 9 hits.
    PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. "MPL1, a novel phosphatase with leucine-rich repeats, is essential for proper ERK2 phosphorylation and cell motility."
      Rodriguez M., Kim B., Lee N.-S., Veeranki S., Kim L.
      Eukaryot. Cell 7:958-966(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiMPL1_DICDI
    AccessioniPrimary (citable) accession number: Q55CS7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3