Reviewed, UniProtKB/Swiss-Prot Q55CQ6 (SERC_DICDI)

Names and origin

Protein names

Recommended name:
    Probable phosphoserine aminotransferase
      Short name=PSAT
    EC=2.6.1.52
Alternative name(s):
    Phosphohydroxythreonine aminotransferase

Gene names

Name:	serC
ORF Names:	DDB_G0269950

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine By similarity.
Catalytic activity	O-phospho-L-serine + 2-oxoglutarate = 3-phosphonooxypyruvate + L-glutamate. 4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate.
Cofactor	Binds 1 pyridoxal phosphate per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 2/3. Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 3/5.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Serine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	O-phospho-L-serine:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 374	374	Probable phosphoserine aminotransferase		PRO_0000327722
Regions
	Region	82 – 83	2	Pyridoxal phosphate binding By similarity
	Region	248 – 249	2	Pyridoxal phosphate binding By similarity
Sites
	Binding site	48	1	L-glutamate By similarity
	Binding site	110	1	Pyridoxal phosphate By similarity
	Binding site	160	1	Pyridoxal phosphate By similarity
	Binding site	183	1	Pyridoxal phosphate By similarity
	Binding site	206	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	207	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q55CQ6-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 2AD0067249179238	FASTA	374	41,992
	10 20 30 40 50 60 MTENINEFRV NNFGAGPGCI PTEVLLEAQK ELLNFQGCGK SIMEVSHRGK EFEGVINETK 70 80 90 100 110 120 SNLKKLLSIS DDYDILFLQG GASSLFAGIP MNLCENGVED IVDFIVTGSW SKQASNDGKY 130 140 150 160 170 180 FCKVNKVVDM EKEKFLTVTE PQSWKFSPDA KYVHYCDNET IHGIEMPIST PDHLPSNLIK 190 200 210 220 230 240 VCDMSSNFLS KPIDVNKFDL IFAGAQKNAG ISGITIVIIK KSLLLKTKPN VPSVFNFLKK 250 260 270 280 290 300 SQNNSLDNTP PTFNIYITGL ILKWIINKGG LSEIEKLNIA KAHALYEYID NSNSFYKCSI 310 320 330 340 350 360 DKNYRSRMNV VFRIQDGNTE LEEKFIKEAS KENITDIKGH RSVGGLRVSL YNAITIDQTL 370 ILINFMTNFH NNNK

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000005 Genomic DNA. Translation: EAL72325.1.
RefSeq	XP_646425.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q55CQ6.
Genome annotation databases
GeneID	3397355.
GenomeReviews	Gene locus serC in contig CM000150_GR.
KEGG	ddi:DDB_0230053.
Organism-specific databases
dictyBase	DDB_G0269950. serC.
Phylogenomic databases
OMA	SMYNTPP.
Enzyme and pathway databases
BRENDA	2.6.1.52. 424.
Family and domain databases
InterPro	IPR000192. Aminotrans_V/Cys_dSase. IPR020578. Aminotrans_V_PyrdxlP_BS. IPR003248. Pser_amintransf. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
Pfam	PF00266. Aminotran_5. 1 hit. [Graphical view]
ProDom	PD001544. Pser_amintransf. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01364. serC_1. 1 hit.
PROSITE	PS00595. AA_TRANSFER_CLASS_5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SERC_DICDI

Accession

Primary (citable) accession number: Q55CQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	November 3, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)