Reviewed, UniProtKB/Swiss-Prot Q55C99 (SYDM_DICDI)

Names and origin

Protein names

Recommended name:
    Aspartyl-tRNA synthetase, mitochondrial
    EC=6.1.1.12
Alternative name(s):
    Aspartate--tRNA ligase
      Short name=AspRS

Gene names

Name:	maspS
ORF Names:	DDB_G0270152

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	692 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp).
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 61	61	Mitochondrion Potential
	Chain	62 – 692	631	Aspartyl-tRNA synthetase, mitochondrial		PRO_0000327861
Regions
	Compositional bias	14 – 72	59	Asn-rich

Sequences

	Sequence		Length	Mass (Da)
	Q55C99-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 0028309770FF7FD1	FASTA	692	79,277
	10 20 30 40 50 60 MNRVILKDSK IFLNVLNKPI IKNKNCLSLL NITTSSTTSI IKNQQINQFN KRNFTNTINN 70 80 90 100 110 120 NKNENINNKI LNIIERSHSC GEITSKDIGK EVIIYGWINS LRNLGDNVFL VIRDGHGKVQ 130 140 150 160 170 180 CYVDLKQQCI LKSSVPNIDI NERNSIEENI KLFKLESIVS IKGKVIARPE RMVNKNMSTG 190 200 210 220 230 240 EIEISVDQLQ LLNNCVDLPF TVEHDSTAVS EELRLKYRYV DLRRDKVQSN IRLRSKVAMA 250 260 270 280 290 300 ARNYLINQQF IEVETPTLFR PTPEGAREYL VPTRHQGQFY SLPQSPQQYK QLLMVGGIDR 310 320 330 340 350 360 YFQLARCYRD EDLRSDRQPE FTQIDMELSF VNTQMIYRII EGLVKTLWKE AGFNIDYEFP 370 380 390 400 410 420 FYTYEQVLST YGIDKPDTRY DMKLVDITDC FNKDETNINL FKNALSQASN NFKESKPVIK 430 440 450 460 470 480 CIKLDQVLPT LKSKHLDQIT TESNSIITVQ IKSNNEWKSL ISKSISEQEK TLITERMNLK 490 500 510 520 530 540 EGDVLLISVG PRFQVESTLG KTRIYCANLL KELNLLKLDP QQFNFLWVVD FPLFTPSDYM 550 560 570 580 590 600 NEQSALLSTH HPFTAPHPED IDLLLNPLST PSDYSKIRGQ HYDIVINGVE LGGGSIRIHN 610 620 630 640 650 660 SDVQLRVLEK VLKLEPHMVQ RFNHLLTALS MGCPPHGGIA LGFDRLCSLL VNSNSIRDVI 670 680 690 AFPKTSGGKE LMTSSPATVT KSELDELFLI QK

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000005 Genomic DNA. Translation: EAL72426.1.
RefSeq	XP_646582.1.
3D structure databases
HSSP	HSSP built from PDB template 1BBW based on UniProtKB P0A8N3.
SMR	Q55C99. Positions 74-689.
ModBase	Search...
Protein-protein interaction databases
STRING	Q55C99.
Genome annotation databases
GeneID	8617552.
KEGG	ddi:DDB_0231311.
Organism-specific databases
dictyBase	DDB_G0270152. maspS.
Phylogenomic databases
eggNOG	KOG2411.
HOGENOM	HBG396032.
OMA	PLFEYDE.
PhylomeDB	Q55C99.
Enzyme and pathway databases
BRENDA	6.1.1.12. 424.
Family and domain databases
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-dom. IPR002312. Asp-tRNA-synth_IIb. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR018153. Asp-tRNA-synth_IIb_C_bac/mt. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00459. aspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYDM_DICDI

Accession

Primary (citable) accession number: Q55C99

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	February 9, 2010
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)