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Protein

Ubiquitin thioesterase OTU1

Gene

yod1

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Hydrolase that can remove conjugated ubiquitin from proteins and may therefore play an important regulatory role at the level of protein turnover by preventing degradation.By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei131By similarity1
Active sitei134NucleophileBy similarity1
Binding sitei238Substrate; via carbonyl oxygenBy similarity1
Active sitei239By similarity1
Active sitei316By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri292 – 316C2H2-typeAdd BLAST25

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DDI-5689896. Ovarian tumor domain proteases.

Protein family/group databases

MEROPSiC85.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin thioesterase OTU1 (EC:3.4.19.12By similarity)
Gene namesi
Name:yod1
ORF Names:DDB_G0271346
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 2, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0271346. yod1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003283671 – 325Ubiquitin thioesterase OTU1Add BLAST325

Proteomic databases

PaxDbiQ55BI3.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0266410.

Structurei

3D structure databases

ProteinModelPortaliQ55BI3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini123 – 246OTUPROSITE-ProRule annotationAdd BLAST124

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni7 – 86UBX-likeBy similarityAdd BLAST80
Regioni128 – 134Cys-loopBy similarity7
Regioni185 – 195Variable-loopBy similarityAdd BLAST11
Regioni235 – 239His-loopBy similarity5
Regioni265 – 270S2 siteBy similarity6

Sequence similaritiesi

Contains 1 C2H2-type zinc finger.Curated
Contains 1 OTU domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri292 – 316C2H2-typeAdd BLAST25

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3288. Eukaryota.
COG5539. LUCA.
InParanoidiQ55BI3.
KOiK13719.
OMAiKFTLRCM.
PhylomeDBiQ55BI3.

Family and domain databases

InterProiIPR003323. OTU_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50802. OTU. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q55BI3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLISIRIRS KTGVENIKLE SQLKLKELQN IIEEKTKIST DTQKILYGFP
60 70 80 90 100
PKALDLSNQD AVISGFLANG DTITIENVSS ISSNPGVDSV TNDKVTSSTN
110 120 130 140 150
SFDRNIKSQP FISQEEEEDG GYATRRVTDD DNSCLFSAVA YVLEDKNRLK
160 170 180 190 200
GYSLRALIAQ NVKSDPFEYN EAVLGKSNEG YCNWIQNPKN WGGAIELSIL
210 220 230 240 250
SNHYKVEIAA FDISTQLMYC YGEDRKYTER VYLIYDGIHY DALSICLTKN
260 270 280 290 300
GPEDFDITRF SVDDKDSLAK MKVLIEKEFK AGKFTDTAKF SLICLNCNKT
310 320
LKGEKEAAIH ASTTGHGNFT EYKKR
Length:325
Mass (Da):36,456
Last modified:May 24, 2005 - v1
Checksum:iDFB2AB7B181E4BDD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000006 Genomic DNA. Translation: EAL71803.1.
RefSeqiXP_645629.1. XM_640537.1.

Genome annotation databases

EnsemblProtistsiEAL71803; EAL71803; DDB_G0271346.
GeneIDi8617821.
KEGGiddi:DDB_G0271346.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000006 Genomic DNA. Translation: EAL71803.1.
RefSeqiXP_645629.1. XM_640537.1.

3D structure databases

ProteinModelPortaliQ55BI3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0266410.

Protein family/group databases

MEROPSiC85.007.

Proteomic databases

PaxDbiQ55BI3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL71803; EAL71803; DDB_G0271346.
GeneIDi8617821.
KEGGiddi:DDB_G0271346.

Organism-specific databases

dictyBaseiDDB_G0271346. yod1.

Phylogenomic databases

eggNOGiKOG3288. Eukaryota.
COG5539. LUCA.
InParanoidiQ55BI3.
KOiK13719.
OMAiKFTLRCM.
PhylomeDBiQ55BI3.

Enzyme and pathway databases

ReactomeiR-DDI-5689896. Ovarian tumor domain proteases.

Miscellaneous databases

PROiQ55BI3.

Family and domain databases

InterProiIPR003323. OTU_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50802. OTU. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOTU1_DICDI
AccessioniPrimary (citable) accession number: Q55BI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: November 30, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.