Q55BI3 (OTU1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin thioesterase OTU1
EC=3.4.19.12

Gene names

Name:	yod1
ORF Names:	DDB_G0271346

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Hydrolase that can remove conjugated ubiquitin from proteins and may therefore play an important regulatory role at the level of protein turnover by preventing degradation By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Sequence similarities	Contains 1 C2H2-type zinc finger. Contains 1 OTU domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein deubiquitination Inferred from sequence or structural similarity. Source: dictyBase proteolysis Inferred from electronic annotation. Source: UniProtKB-KW sorocarp development Inferred from mutant phenotype PubMed 17659086. Source: dictyBase
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-specific protease activity Inferred from sequence or structural similarity. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	Ubiquitin thioesterase OTU1		PRO_0000328367
Regions
	Domain	123 – 246	124	OTU
	Zinc finger	292 – 316	25	C2H2-type
Sites
	Active site	131	1	By similarity
	Active site	134	1	Nucleophile By similarity
	Active site	316	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q55BI3 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: DFB2AB7B181E4BDD	FASTA	325	36,456
	10 20 30 40 50 60 MSLISIRIRS KTGVENIKLE SQLKLKELQN IIEEKTKIST DTQKILYGFP PKALDLSNQD 70 80 90 100 110 120 AVISGFLANG DTITIENVSS ISSNPGVDSV TNDKVTSSTN SFDRNIKSQP FISQEEEEDG 130 140 150 160 170 180 GYATRRVTDD DNSCLFSAVA YVLEDKNRLK GYSLRALIAQ NVKSDPFEYN EAVLGKSNEG 190 200 210 220 230 240 YCNWIQNPKN WGGAIELSIL SNHYKVEIAA FDISTQLMYC YGEDRKYTER VYLIYDGIHY 250 260 270 280 290 300 DALSICLTKN GPEDFDITRF SVDDKDSLAK MKVLIEKEFK AGKFTDTAKF SLICLNCNKT 310 320 LKGEKEAAIH ASTTGHGNFT EYKKR

References

[1]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000006 Genomic DNA. Translation: EAL71803.1.
RefSeq	XP_645629.1. XM_640537.1.
3D structure databases
ProteinModelPortal	Q55BI3.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDBDRAFT_0202882.
Protein family/group databases
MEROPS	C85.007.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0266410; DDB0266410; DDB_G0271346.
GeneID	8617821.
KEGG	ddi:DDB_G0271346.
Organism-specific databases
dictyBase	DDB_G0271346. yod1.
Phylogenomic databases
eggNOG	COG5539.
KO	K13719.
OMA	PIQSGDM.
ProtClustDB	CLSZ2497279.
Family and domain databases
InterPro	IPR003323. OTU. [Graphical view]
Pfam	PF02338. OTU. 1 hit. [Graphical view]
PROSITE	PS50802. OTU. 1 hit. PS00028. ZINC_FINGER_C2H2_1. 1 hit. PS50157. ZINC_FINGER_C2H2_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTU1_DICDI

Accession

Primary (citable) accession number: Q55BI3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	May 1, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)