Reviewed, UniProtKB/Swiss-Prot Q55BI2 (IDHA_DICDI)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase [NAD] regulatory subunit A, mitochondrial
    EC=1.1.1.41

Gene names

Name:	idhA
ORF Names:	DDB_G0271344

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Performs an essential role in the oxidative function of the citric acid cycle By similarity.
Catalytic activity	Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Subunit structure	Heteroligomer of catalytic and regulatory subunits By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isocitrate metabolic process Inferred from sequence or structural similarity. Source: UniProtKB oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NAD+) activity Inferred from sequence or structural similarity. Source: UniProtKB magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 354		Isocitrate dehydrogenase [NAD] regulatory subunit A, mitochondrial		PRO_0000328018
Regions
	Nucleotide binding	276 – 282	7	NADP By similarity
Sites
	Metal binding	243	1	Magnesium or manganese By similarity
	Binding site	95	1	Substrate By similarity
	Binding site	97	1	Substrate By similarity
	Binding site	101	1	Substrate By similarity
	Binding site	111	1	Substrate By similarity
	Binding site	132	1	Substrate By similarity
	Binding site	289	1	NADP; via amide nitrogen and carbonyl oxygen By similarity
	Site	139	1	Critical for catalysis By similarity
	Site	186	1	Critical for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q55BI2-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 07BC0DC939425EBE	FASTA	354	38,341
	10 20 30 40 50 60 MFSRKSLSIF STLRNYSSST SKIQKVTLIP GDGIGPEISE SVKRVFSAVK APIEWETVVV 70 80 90 100 110 120 DANTGISKEV IESISKNKIG LKGPISTPIG TGHQSLNLGL RKTFNLYANI RPCLSIPGHK 130 140 150 160 170 180 TRYNNVNTVV VRENTEGEYS GIENQPVKGV AQSIKIITKE ASTRIAHYAF QYALANGRKK 190 200 210 220 230 240 VTCIHKANIM KQSDGLFVKS CREVSTRYPS IKYEELTIDN NCMQLVLDPN QMDVMVLPNL 250 260 270 280 290 300 YGDIVSDLCA GLIGGLGLTP SGNIGENGSA IFEAVHGTAP DIAGKNKANP TALILSSIMM 310 320 330 340 350 LRHLGHFHEA SIIENAVLNT LTEGKVKTGD LGGNSSCSEY TDELVKKITE SLNK

References

[1]

"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

[2]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000006 Genomic DNA. Translation: EAL71802.1.
RefSeq	XP_645630.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3396844.
KEGG	ddi:DDB_0231288.
Organism-specific databases
dictyBase	DDB_G0271344. idhA.
Phylogenomic databases
OMA	Q55BI2. GGNSKCS.
Enzyme and pathway databases
BRENDA	1.1.1.41. 424.
Family and domain databases
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004434. Isocitrate_DH_NAD_mit. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00175. mito_nad_idh. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IDHA_DICDI

Accession

Primary (citable) accession number: Q55BI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)