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Q55BI2

- IDHA_DICDI

UniProt

Q55BI2 - IDHA_DICDI

Protein

Isocitrate dehydrogenase [NAD] regulatory subunit A, mitochondrial

Gene

idhA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
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    Functioni

    Performs an essential role in the oxidative function of the citric acid cycle.By similarity

    Catalytic activityi

    Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei95 – 951SubstrateBy similarity
    Binding sitei97 – 971SubstrateBy similarity
    Binding sitei101 – 1011SubstrateBy similarity
    Binding sitei111 – 1111SubstrateBy similarity
    Binding sitei132 – 1321SubstrateBy similarity
    Sitei139 – 1391Critical for catalysisBy similarity
    Sitei186 – 1861Critical for catalysisBy similarity
    Metal bindingi243 – 2431Magnesium or manganeseBy similarity
    Binding sitei289 – 2891NADP; via amide nitrogen and carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi276 – 2827NADPBy similarity

    GO - Molecular functioni

    1. isocitrate dehydrogenase (NAD+) activity Source: UniProtKB
    2. magnesium ion binding Source: InterPro
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. isocitrate metabolic process Source: UniProtKB
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, NAD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NAD] regulatory subunit A, mitochondrial (EC:1.1.1.41)
    Gene namesi
    Name:idhA
    ORF Names:DDB_G0271344
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0271344. idhA.

    Subcellular locationi

    Mitochondrion By similarity

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 354Isocitrate dehydrogenase [NAD] regulatory subunit A, mitochondrialPRO_0000328018
    Transit peptidei1 – ?MitochondrionSequence Analysis

    Proteomic databases

    PRIDEiQ55BI2.

    Interactioni

    Subunit structurei

    Heterooligomer of catalytic and regulatory subunits.By similarity

    Protein-protein interaction databases

    STRINGi44689.DDB_0231288.

    Structurei

    3D structure databases

    ProteinModelPortaliQ55BI2.
    SMRiQ55BI2. Positions 21-348.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0473.
    KOiK00030.
    OMAiTEYTEAI.
    PhylomeDBiQ55BI2.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR001804. Isocitrate/isopropylmalate_DH.
    IPR004434. Isocitrate_DH_NAD.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11835. PTHR11835. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q55BI2-1 [UniParc]FASTAAdd to Basket

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    MFSRKSLSIF STLRNYSSST SKIQKVTLIP GDGIGPEISE SVKRVFSAVK    50
    APIEWETVVV DANTGISKEV IESISKNKIG LKGPISTPIG TGHQSLNLGL 100
    RKTFNLYANI RPCLSIPGHK TRYNNVNTVV VRENTEGEYS GIENQPVKGV 150
    AQSIKIITKE ASTRIAHYAF QYALANGRKK VTCIHKANIM KQSDGLFVKS 200
    CREVSTRYPS IKYEELTIDN NCMQLVLDPN QMDVMVLPNL YGDIVSDLCA 250
    GLIGGLGLTP SGNIGENGSA IFEAVHGTAP DIAGKNKANP TALILSSIMM 300
    LRHLGHFHEA SIIENAVLNT LTEGKVKTGD LGGNSSCSEY TDELVKKITE 350
    SLNK 354
    Length:354
    Mass (Da):38,341
    Last modified:May 24, 2005 - v1
    Checksum:i07BC0DC939425EBE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000006 Genomic DNA. Translation: EAL71802.1.
    RefSeqiXP_645630.1. XM_640538.1.

    Genome annotation databases

    EnsemblProtistsiDDB0231288; DDB0231288; DDB_G0271344.
    GeneIDi8617822.
    KEGGiddi:DDB_G0271344.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000006 Genomic DNA. Translation: EAL71802.1 .
    RefSeqi XP_645630.1. XM_640538.1.

    3D structure databases

    ProteinModelPortali Q55BI2.
    SMRi Q55BI2. Positions 21-348.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0231288.

    Proteomic databases

    PRIDEi Q55BI2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0231288 ; DDB0231288 ; DDB_G0271344 .
    GeneIDi 8617822.
    KEGGi ddi:DDB_G0271344.

    Organism-specific databases

    dictyBasei DDB_G0271344. idhA.

    Phylogenomic databases

    eggNOGi COG0473.
    KOi K00030.
    OMAi TEYTEAI.
    PhylomeDBi Q55BI2.

    Miscellaneous databases

    PROi Q55BI2.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR001804. Isocitrate/isopropylmalate_DH.
    IPR004434. Isocitrate_DH_NAD.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11835. PTHR11835. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00175. mito_nad_idh. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiIDHA_DICDI
    AccessioniPrimary (citable) accession number: Q55BI2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3