Q55BH9 (DGAT1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Diacylglycerol O-acyltransferase 1
EC=2.3.1.20

Gene names

Name:	dgat1
ORF Names:	DDB_G0271342

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	617 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates By similarity.
Catalytic activity	Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.
Pathway	Lipid metabolism; glycerolipid metabolism.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.

Ontologies

Keywords
Biological process	Glycerol metabolism Lipid metabolism Lipid synthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycerol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW triglyceride biosynthetic process Inferred from sequence or structural similarity. Source: dictyBase
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-acylglycerol O-acyltransferase activity Inferred from sequence or structural similarity. Source: dictyBase diacylglycerol O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 617	617	Diacylglycerol O-acyltransferase 1		PRO_0000377488
Regions
	Transmembrane	217 – 237	21	Helical; Potential
	Transmembrane	254 – 274	21	Helical; Potential
	Transmembrane	306 – 326	21	Helical; Potential
	Transmembrane	399 – 419	21	Helical; Potential
	Transmembrane	449 – 469	21	Helical; Potential
	Transmembrane	520 – 540	21	Helical; Potential
	Transmembrane	545 – 565	21	Helical; Potential
	Transmembrane	570 – 590	21	Helical; Potential
	Compositional bias	18 – 35	18	Poly-Gln
	Compositional bias	96 – 104	9	Poly-Asn
	Compositional bias	153 – 158	6	Poly-His
	Compositional bias	159 – 177	19	Poly-Asn
Sites
	Active site	532	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q55BH9 [UniParc]. Last modified June 16, 2009. Version 2. Checksum: DCD8FD193A3023D7	FASTA	617	71,946
	10 20 30 40 50 60 MEPIPPSNGN KNNSMDKQPQ QPQQPQQQQQ QQQQQRRDQR NSKLNELNET ERVRNRFISH 70 80 90 100 110 120 EFHKLDRTKS RIDAPKISFS DSESESDSEF FLAKRNTNNN NQNNTSPTFS SANGKQSNLT 130 140 150 160 170 180 QRKINTQIQS KQPTNNNVQP LTDDEGTINH SNHHHHHHNQ NNNGNNNNNN NNNNNNNKIS 190 200 210 220 230 240 TPPKQEEKMT MNGLFTLRPS ILSSESNGSS YRGFLNLLLI LLITASFRLV ILNHLLYGIR 250 260 270 280 290 300 INLDLYKISE YHRWPGVMIS LMINLFIIAA YLIEKAAAKQ LLPDRICYLL RIINCAAVII 310 320 330 340 350 360 VPSGSIIAFS PNPASGIIVM ILICTFSMKI ISYAYENSKQ RKLNPDNKKF VIDPTNTSIY 370 380 390 400 410 420 PNNLSLRSTY WFMLVPTLVY QLSYPRSPKI RKGYLLRRIV EALSLSLLIL WMVNQYMLPL 430 440 450 460 470 480 VQNSIEPLEK IDIVLIVERI MKLSLPNLYV WLLGFYVFFH LYLNIVAEIT RFGDREFYRD 490 500 510 520 530 540 WWNSTGLDYF WRTWNMPVHH WMVVLIYTPM RRRGFSKNMG YFMCFFVSAI FHELVISIPF 550 560 570 580 590 600 HSLKLWGFFG IMSQMVLIAL TKNLMNGRNL GNVIFWISIV LGQPLVVLLY YRNFVLENPE 610 WYRNVEPPTS PPVMPFY

References

[1]

"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

[2]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000006 Genomic DNA. Translation: EAL71801.2.
RefSeq	XP_645633.2. XM_640541.2.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0304727; DDB0304727; DDB_G0271342.
GeneID	8617825.
GenomeReviews	Gene locus dgat1 in contig CM000151_GR.
KEGG	ddi:DDB_G0271342.
Organism-specific databases
dictyBase	DDB_G0271342. dgat1.
Phylogenomic databases
eggNOG	KOG0380.
GeneTree	EPrGT00050000002160.
HOGENOM	HBG326701.
InParanoid	Q55BH9.
OMA	LTQQWMV.
ProtClustDB	CLSZ2736090.
Family and domain databases
InterPro	IPR004299. MBOAT_fam. IPR014371. Oat_ACAT_DAG_ARE. [Graphical view]
KO	K11155.
PANTHER	PTHR10408. PTHR10408. 1 hit.
Pfam	PF03062. MBOAT. 1 hit. [Graphical view]
PIRSF	PIRSF000439. Oat_ACAT_DAG_ARE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DGAT1_DICDI

Accession

Primary (citable) accession number: Q55BH9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2009
Last sequence update:	June 16, 2009
Last modified:	November 16, 2011
This is version 40 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)