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Q55B10

- AGAL_DICDI

UniProt

Q55B10 - AGAL_DICDI

Protein

Probable alpha-galactosidase

Gene

melA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (02 May 2006)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei147 – 1471NucleophileBy similarity
    Active sitei202 – 2021Proton donorBy similarity

    GO - Molecular functioni

    1. alpha-galactosidase activity Source: RefGenome
    2. raffinose alpha-galactosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycoside catabolic process Source: RefGenome
    2. glycosylceramide catabolic process Source: RefGenome
    3. oligosaccharide metabolic process Source: RefGenome

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable alpha-galactosidase (EC:3.2.1.22)
    Alternative name(s):
    Alpha-D-galactoside galactohydrolase
    Melibiase
    Gene namesi
    Name:melA
    ORF Names:DDB_G0271490
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0271490. melA.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: RefGenome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 385366Probable alpha-galactosidasePRO_0000327851Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi40 ↔ 72By similarity
    Disulfide bondi119 ↔ 149By similarity

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDBDRAFT_0216854.

    Structurei

    3D structure databases

    ProteinModelPortaliQ55B10.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni180 – 1845Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 27 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG68897.
    KOiK07407.
    OMAiTYAEWGI.
    PhylomeDBiQ55B10.

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR013780. Glyco_hydro_13_b.
    IPR002241. Glyco_hydro_27.
    IPR000111. Glyco_hydro_GHD.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02065. Melibiase. 1 hit.
    [Graphical view]
    PRINTSiPR00740. GLHYDRLASE27.
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q55B10-1 [UniParc]FASTAAdd to Basket

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    MMKIAATLLA TIALATVNAL DNGLALTPQM GWSSWNFYAC NINESVIMNT    50
    AKAMVSNGMA DAGYTYVNID DCWAGGRYPN GTVYADPTNF PNGIKYVADY 100
    IHSLGLKIGI YTDAGTETCQ KRVGSYGYEA NDAQTYAEWG IDYVKEDWCY 150
    ATLENPQQRY QIMSQALNAT GRPMFFSLCD WGYENPWTFG MSVGNSWRTT 200
    PDIKDNWDSM LSNLMAQAPI TSFSGIGGFN DPDMMMVGNG GMSNTEYVSH 250
    FSLWSLLNAP LIAGCDLIDI DQETLSILTA SEVIAINQDP LGVQGSLVKS 300
    YNGGLQQIWA KPLSNGARAV VLFNTDTNPA TIELLWGNIW MEPSQQLSIR 350
    NLWTQTNLGT FTESYESDSL IPPHGCIMLT LTPTN 385
    Length:385
    Mass (Da):42,312
    Last modified:May 2, 2006 - v1
    Checksum:i873DE98AEEB766BE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000006 Genomic DNA. Translation: EAL71875.1.
    RefSeqiXP_645802.1. XM_640710.1.

    Genome annotation databases

    EnsemblProtistsiDDB0233870; DDB0233870; DDB_G0271490.
    GeneIDi8617994.
    KEGGiddi:DDB_G0271490.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000006 Genomic DNA. Translation: EAL71875.1 .
    RefSeqi XP_645802.1. XM_640710.1.

    3D structure databases

    ProteinModelPortali Q55B10.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDBDRAFT_0216854.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0233870 ; DDB0233870 ; DDB_G0271490 .
    GeneIDi 8617994.
    KEGGi ddi:DDB_G0271490.

    Organism-specific databases

    dictyBasei DDB_G0271490. melA.

    Phylogenomic databases

    eggNOGi NOG68897.
    KOi K07407.
    OMAi TYAEWGI.
    PhylomeDBi Q55B10.

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR013780. Glyco_hydro_13_b.
    IPR002241. Glyco_hydro_27.
    IPR000111. Glyco_hydro_GHD.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02065. Melibiase. 1 hit.
    [Graphical view ]
    PRINTSi PR00740. GLHYDRLASE27.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiAGAL_DICDI
    AccessioniPrimary (citable) accession number: Q55B10
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: May 2, 2006
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3