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Reviewed, UniProtKB/Swiss-Prot Q55B10 (AGAL_DICDI)

Last modified November 3, 2009. Version 28. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Probable alpha-galactosidase
    EC=3.2.1.22
Alternative name(s):
    Alpha-D-galactoside galactohydrolase
    Melibiase
Gene names
Name: melA
ORF Names: DDB_G0271490
OrganismDictyostelium discoideum (Slime mold) [Complete proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length385 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology.

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactohydrolase.

Sequence similarities

Belongs to the glycosyl hydrolase 27 family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionalpha-galactosidase activity

Inferred from electronic annotation. Source: EC

cation binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 385366Probable alpha-galactosidase
PRO_0000327851

Regions

Region180 – 1845Substrate binding By similarity

Sites

Active site1471Nucleophile By similarity
Active site2021Proton donor By similarity

Amino acid modifications

Disulfide bond40 ↔ 72 By similarity
Disulfide bond119 ↔ 149 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q55B10-1 [UniParc].

Last modified May 2, 2006. Version 1.
Checksum: 873DE98AEEB766BE

FASTA38542,312
        10         20         30         40         50         60 
MMKIAATLLA TIALATVNAL DNGLALTPQM GWSSWNFYAC NINESVIMNT AKAMVSNGMA 

        70         80         90        100        110        120 
DAGYTYVNID DCWAGGRYPN GTVYADPTNF PNGIKYVADY IHSLGLKIGI YTDAGTETCQ 

       130        140        150        160        170        180 
KRVGSYGYEA NDAQTYAEWG IDYVKEDWCY ATLENPQQRY QIMSQALNAT GRPMFFSLCD 

       190        200        210        220        230        240 
WGYENPWTFG MSVGNSWRTT PDIKDNWDSM LSNLMAQAPI TSFSGIGGFN DPDMMMVGNG 

       250        260        270        280        290        300 
GMSNTEYVSH FSLWSLLNAP LIAGCDLIDI DQETLSILTA SEVIAINQDP LGVQGSLVKS 

       310        320        330        340        350        360 
YNGGLQQIWA KPLSNGARAV VLFNTDTNPA TIELLWGNIW MEPSQQLSIR NLWTQTNLGT 

       370        380 
FTESYESDSL IPPHGCIMLT LTPTN 

« Hide

References

[1]"Sequence and analysis of chromosome 2 of Dictyostelium discoideum."
Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A.
Nature 418:79-85(2002) [PubMed: 12097910] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed: 15875012] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.

Cross-references

Sequence databases

AAFI02000006 Genomic DNA. Translation: EAL71875.1.
AAFI02000226 Genomic DNA. Translation: EAL60436.1.
RefSeqXP_628847.1.
XP_645802.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID3396917.
5860621.
GenomeReviewsGene locus melA in contig CM000151_GR.
KEGGddi:DDBDRAFT_0215062.
ddi:DDB_0233870.

Organism-specific databases

dictyBaseDDB_G0271490. melA.

Phylogenomic databases

OMACWMAPQR.

Enzyme and pathway databases

BRENDA3.2.1.22. 424.

Family and domain databases

InterProIPR013785. Aldolase_TIM.
IPR013780. Glyco_hydro_13_b.
IPR002241. Glyco_hydro_27.
IPR000111. Glyco_hydro_GHD.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit.
PfamPF02065. Melibiase. 1 hit.
[Graphical view]
PRINTSPR00740. GLHYDRLASE27.
ProDomPD002572. Glyco_hydro_GHD. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Entry information

Entry nameAGAL_DICDI
AccessionPrimary (citable) accession number: Q55B10
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 2, 2006
Last modified: November 3, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents