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Q55B10 (AGAL_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable alpha-galactosidase

EC=3.2.1.22
Alternative name(s):
Alpha-D-galactoside galactohydrolase
Melibiase
Gene names
Name:melA
ORF Names:DDB_G0271490
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length385 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.

Sequence similarities

Belongs to the glycosyl hydrolase 27 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 385366Probable alpha-galactosidase
PRO_0000327851

Regions

Region180 – 1845Substrate binding By similarity

Sites

Active site1471Nucleophile By similarity
Active site2021Proton donor By similarity

Amino acid modifications

Disulfide bond40 ↔ 72 By similarity
Disulfide bond119 ↔ 149 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q55B10 [UniParc].

Last modified May 2, 2006. Version 1.
Checksum: 873DE98AEEB766BE

FASTA38542,312
        10         20         30         40         50         60 
MMKIAATLLA TIALATVNAL DNGLALTPQM GWSSWNFYAC NINESVIMNT AKAMVSNGMA 

        70         80         90        100        110        120 
DAGYTYVNID DCWAGGRYPN GTVYADPTNF PNGIKYVADY IHSLGLKIGI YTDAGTETCQ 

       130        140        150        160        170        180 
KRVGSYGYEA NDAQTYAEWG IDYVKEDWCY ATLENPQQRY QIMSQALNAT GRPMFFSLCD 

       190        200        210        220        230        240 
WGYENPWTFG MSVGNSWRTT PDIKDNWDSM LSNLMAQAPI TSFSGIGGFN DPDMMMVGNG 

       250        260        270        280        290        300 
GMSNTEYVSH FSLWSLLNAP LIAGCDLIDI DQETLSILTA SEVIAINQDP LGVQGSLVKS 

       310        320        330        340        350        360 
YNGGLQQIWA KPLSNGARAV VLFNTDTNPA TIELLWGNIW MEPSQQLSIR NLWTQTNLGT 

       370        380 
FTESYESDSL IPPHGCIMLT LTPTN 

« Hide

References

[1]"Sequence and analysis of chromosome 2 of Dictyostelium discoideum."
Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A.
Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000006 Genomic DNA. Translation: EAL71875.1.
RefSeqXP_645802.1. XM_640710.1.

3D structure databases

ProteinModelPortalQ55B10.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDBDRAFT_0216854.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0233870; DDB0233870; DDB_G0271490.
GeneID8617994.
KEGGddi:DDB_G0271490.

Organism-specific databases

dictyBaseDDB_G0271490. melA.

Phylogenomic databases

eggNOGNOG68897.
OMATYAEWGI.
PhylomeDBQ55B10.

Family and domain databases

Gene3D2.60.40.1180. 1 hit.
3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR013780. Glyco_hydro_13_b.
IPR002241. Glyco_hydro_27.
IPR000111. Glyco_hydro_GHD.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02065. Melibiase. 1 hit.
[Graphical view]
PRINTSPR00740. GLHYDRLASE27.
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameAGAL_DICDI
AccessionPrimary (citable) accession number: Q55B10
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 2, 2006
Last modified: July 9, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase