Reviewed, UniProtKB/Swiss-Prot Q55B10 (AGAL_DICDI)

Names and origin

Protein names

Recommended name:
    Probable alpha-galactosidase
    EC=3.2.1.22
Alternative name(s):
    Alpha-D-galactoside galactohydrolase
    Melibiase

Gene names

Name:	melA
ORF Names:	DDB_G0271490

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactohydrolase.
Sequence similarities	Belongs to the glycosyl hydrolase 27 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 385	366	Probable alpha-galactosidase		PRO_0000327851
Regions
	Region	180 – 184	5	Substrate binding By similarity
Sites
	Active site	147	1	Nucleophile By similarity
	Active site	202	1	Proton donor By similarity
Amino acid modifications
	Disulfide bond	40 ↔ 72		By similarity
	Disulfide bond	119 ↔ 149		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q55B10-1 [UniParc]. Last modified May 2, 2006. Version 1. Checksum: 873DE98AEEB766BE	FASTA	385	42,312
	10 20 30 40 50 60 MMKIAATLLA TIALATVNAL DNGLALTPQM GWSSWNFYAC NINESVIMNT AKAMVSNGMA 70 80 90 100 110 120 DAGYTYVNID DCWAGGRYPN GTVYADPTNF PNGIKYVADY IHSLGLKIGI YTDAGTETCQ 130 140 150 160 170 180 KRVGSYGYEA NDAQTYAEWG IDYVKEDWCY ATLENPQQRY QIMSQALNAT GRPMFFSLCD 190 200 210 220 230 240 WGYENPWTFG MSVGNSWRTT PDIKDNWDSM LSNLMAQAPI TSFSGIGGFN DPDMMMVGNG 250 260 270 280 290 300 GMSNTEYVSH FSLWSLLNAP LIAGCDLIDI DQETLSILTA SEVIAINQDP LGVQGSLVKS 310 320 330 340 350 360 YNGGLQQIWA KPLSNGARAV VLFNTDTNPA TIELLWGNIW MEPSQQLSIR NLWTQTNLGT 370 380 FTESYESDSL IPPHGCIMLT LTPTN

References

[1]

"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

[2]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000006 Genomic DNA. Translation: EAL71875.1. AAFI02000226 Genomic DNA. Translation: EAL60436.1.
RefSeq	XP_628847.1. XP_645802.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3396917. 5860621.
GenomeReviews	Gene locus melA in contig CM000151_GR.
KEGG	ddi:DDBDRAFT_0215062. ddi:DDB_0233870.
Organism-specific databases
dictyBase	DDB_G0271490. melA.
Phylogenomic databases
OMA	CWMAPQR.
Enzyme and pathway databases
BRENDA	3.2.1.22. 424.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR013780. Glyco_hydro_13_b. IPR002241. Glyco_hydro_27. IPR000111. Glyco_hydro_GHD. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit. G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit.
Pfam	PF02065. Melibiase. 1 hit. [Graphical view]
PRINTS	PR00740. GLHYDRLASE27.
ProDom	PD002572. Glyco_hydro_GHD. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

AGAL_DICDI

Accession

Primary (citable) accession number: Q55B10

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 2, 2006
Last modified:	November 3, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)