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Q55AX2 (DOT1L_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Histone-lysine N-methyltransferase, H3 lysine-79 specific

EC=2.1.1.43
Alternative name(s):
Disruptor of telomeric silencing A
Histone H3-K79 methyltransferase
Short name=H3-K79-HMTase
SAM domain-containing protein
Gene names
Name:dotA
Synonyms:dot1, KMT4
ORF Names:DDB_G0271626
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length1845 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Histone methyltransferase that specifically methylates histone H3 to form H3K79me. This methylation is required for telomere silencing, correct growth and development, and for resistance to DNA damage induced by UV LIGHT. Ref.3

Catalytic activity

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone]. UniProtKB Q04089

Subcellular location

Nucleus By similarity UniProtKB Q04089.

Developmental stage

Expressed during the whole life cycle. Ref.3

Disruption phenotype

Individuals exhibit 4-5 hours delay in development. Development does progress with fewer fruiting bodies and smaller spore heads. No apparent changes in cell cycle regulation. Ref.3

Miscellaneous

In contrast to other lysine histone methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones. Ref.3

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.

Contains 1 DOT1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 18451845Histone-lysine N-methyltransferase, H3 lysine-79 specific
PRO_0000413849

Regions

Domain1125 – 1446322DOT1
Region625 – 63915Required for interaction with nucleosomes and DNA By similarity UniProtKB Q04089
Region1251 – 12544S-adenosyl-L-methionine binding By similarity
Region1274 – 128310S-adenosyl-L-methionine binding By similarity
Compositional bias37 – 7337Asn-rich
Compositional bias90 – 226137Ser-rich
Compositional bias121 – 16040Gln-rich
Compositional bias195 – 26369Asn-rich
Compositional bias336 – 449114Thr-rich
Compositional bias454 – 46916Asn-rich
Compositional bias464 – 676213Arg/Glu/Lys-rich
Compositional bias730 – 77142Asn-rich
Compositional bias832 – 86231Gln-rich
Compositional bias863 – 88018Thr-rich
Compositional bias965 – 1065101Asn-rich
Compositional bias1467 – 1659193Ser-rich
Compositional bias1489 – 150113Thr-rich
Compositional bias1521 – 155636Asn-rich
Compositional bias1772 – 184473Asn-rich

Sites

Binding site13001S-adenosyl-L-methionine By similarity UniProtKB Q04089

Sequences

Sequence LengthMass (Da)Tools
Q55AX2 [UniParc].

Last modified May 2, 2006. Version 2.
Checksum: 6CDF92C0D67AAD87

FASTA1,845212,281
        10         20         30         40         50         60 
MSTNSTPRKQ KLSNSKSLQN SPISPTVKKT NSFPLGNNIP TNINRSKKDK NNNSNNNINN 

        70         80         90        100        110        120 
NNSNGIGSNT IINSTPIATT PVPPLPFIHS SSSSSSSPSP SSSSSSPFPK AKKSPSLSIN 

       130        140        150        160        170        180 
QQQQQQPQQP QQSPQSQQSP QSQQSQQSQQ QQPQQPQEQQ EPLPNLSFLR NQEPDKNVLP 

       190        200        210        220        230        240 
TSRKRPPSVM PSTPNQSSNS SSLNSSLNFS SSNSSPSPTS TQSNNSRFET RSQNDQYENN 

       250        260        270        280        290        300 
NNNNNNNNNN NNNNNNNNNN NNNIECIVID DDDDDDDDEG NSIKSTHTST QSTPIRDRRQ 

       310        320        330        340        350        360 
RDNKWTINPL PQFQREIIDV DTPSPPNESL SIVSQTTTNT ITDTTSIQTP TLIRQSSSLL 

       370        380        390        400        410        420 
SSSSSLSPST TSTPLTQNNI NLQNAQVIAT MTAPMEIELP TIVQLEPLFS SEFTTSTQNL 

       430        440        450        460        470        480 
FIQTPPFTST LTLPTTTAQT SQTLFTIQTS HDINNNNNNN NNNKNKNKNK KEIEKEKEKL 

       490        500        510        520        530        540 
REALKQKLKE YENENEKERE KERKREREIE IERERKERER KEREKERKKE KEREREREER 

       550        560        570        580        590        600 
ERKEIERKER EREEREERER KEIERKETER KEIERKEIER KEIERKERER KERKEREERE 

       610        620        630        640        650        660 
EREREERERK EREEREKEIE MEREKKKEKE KEKEKEKEKE KEKEKEKEKE KEKEKEKEKK 

       670        680        690        700        710        720 
RKENEVENEI EKERREKNDS YMVLNYHHSI DDHHSESESE SDSDQDSIYS ISTQELSSVI 

       730        740        750        760        770        780 
SDNDFCDSDN EKVANNNRTV GETFLNDSRN NNNNNNNNKN NNNKKIENDK NQLIERERLI 

       790        800        810        820        830        840 
SAFNDKAFLY ALDTIREVLG GIDFKLKVSK EQIIEISTNA KKPLNILSEP EQQQQQQQQQ 

       850        860        870        880        890        900 
QQQQHQQQQQ QQQQQQQQQQ QQTTKTTTTT NNTTTTTAET EKPKEVFLLK KPIKIPYGRD 

       910        920        930        940        950        960 
CKKRLIRRFH GTSRNPLFHK NLESTLKLLK RAKFDWASIE FDTKSYLMNC NCKTVCHKSE 

       970        980        990       1000       1010       1020 
RMKDNPTNKL QNNNRNNNNN NNNIINNNNN NNNKNNNNKN NNNKNNNRNN NSIAKKIGTN 

      1030       1040       1050       1060       1070       1080 
NNNNNTTIIK NNNNNNNNNN NNNNNNNNII KNNNNNNKNN NNNNNTIVKK IETIKKDINK 

      1090       1100       1110       1120       1130       1140 
KPTKTTTTTS SSSSSTSSSN SLTVIKKPVK KINGSQRICL FEDDFDVGIG VPVTTGTSET 

      1150       1160       1170       1180       1190       1200 
TTTRASSIRR KMNISNIFDD FTKKPRQNKY NEIEMPDLFA SKECNYTLEQ QAPFIRAQKL 

      1210       1220       1230       1240       1250       1260 
LLTQQHENNL YNGKRFMKYD EWLDLYHGEM RSSIQNPKVL KHYISFSQEV YGEAEPTLLR 

      1270       1280       1290       1300       1310       1320 
HWIHLGLIKP TDVFCDIGCG IGNVLFQLAA QVGCRVIGVE IRKDLYDISQ SMLEIYKKRS 

      1330       1340       1350       1360       1370       1380 
LELGLHPSTQ QIKIYNCDVK GSLEFDFSEP NVFFMHNTCF GPELEISIME LFKKYSKPGT 

      1390       1400       1410       1420       1430       1440 
KVITMKTLCP RFKPSDKKTK PWGIFKYPYE SYEMEEGSLS WRSATNCSFY SFTIDDKDSD 

      1450       1460       1470       1480       1490       1500 
IVTDQTHLNR VILSTPKKKH SKLQLFSSSS LPSSPPSSSS SSSPPNIATN TTTTTTTTTT 

      1510       1520       1530       1540       1550       1560 
TSPSSISLPS PYLSPSKKTP NSNKRDRSDI DNSNSDDGDE NNNNININNN NNNSNNKPIK 

      1570       1580       1590       1600       1610       1620 
LKLSMDHSID NQSNSESSDT DVEYMPWSKR NNRKKRKSLS YSLDSYLSSR ISPSLSLSTS 

      1630       1640       1650       1660       1670       1680 
SSSSSSLDSS PYSSPPSSSS SDNENDDDNG DDEDDSSSSN DTKLKEKLLL MKNNEKIGGA 

      1690       1700       1710       1720       1730       1740 
PPLTRRNANS DTNKLVQGCY QSLSSYALPK EESQIHKLQL QAKLLEHKNS LVLKHQKSIH 

      1750       1760       1770       1780       1790       1800 
DQQKRLSRKQ KKLAKKNKKK EQQLQAQAKT INYNNNNNNN NQNDNQVNHN NLNENEINTD 

      1810       1820       1830       1840 
LINGYNNNNN NNIINNDNDN DNDNDKDDDK DSNNKDYNNI NDNNK 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of Dictyostelium discoideum."
Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A.
Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[3]"The histone methyltransferase Dot1 is required for DNA damage repair and proper development in Dictyostelium."
Muller-Taubenberger A., Bonisch C., Furbringer M., Wittek F., Hake S.B.
Biochem. Biophys. Res. Commun. 404:1016-1022(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
Strain: AX2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000006 Genomic DNA. Translation: EAL71677.2.
RefSeqXP_645574.2. XM_640482.2.

3D structure databases

ProteinModelPortalQ55AX2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDBDRAFT_0216874.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0233511; DDB0233511; DDB_G0271626.
GeneID8618027.
KEGGddi:DDB_G0271626.

Organism-specific databases

dictyBaseDDB_G0271626. dotA.

Phylogenomic databases

eggNOGKOG3924.
InParanoidQ55AX2.
OMALEISIME.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
InterProIPR013110. DOT1.
IPR025789. Histone_H3-K79_MeTrfase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamPF08123. DOT1. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
PROSITEPS51569. DOT1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDOT1L_DICDI
AccessionPrimary (citable) accession number: Q55AX2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 16, 2011
Last sequence update: May 2, 2006
Last modified: June 11, 2014
This is version 56 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase