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Q55AX2

- DOT1L_DICDI

UniProt

Q55AX2 - DOT1L_DICDI

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Protein

Histone-lysine N-methyltransferase, H3 lysine-79 specific

Gene

dotA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Histone methyltransferase that specifically methylates histone H3 to form H3K79me. This methylation is required for telomere silencing, correct growth and development, and for resistance to DNA damage induced by UV LIGHT.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].By similarityPROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei1300 – 13001S-adenosyl-L-methionineBy similarityPROSITE-ProRule annotation

GO - Molecular functioni

  1. histone methyltransferase activity (H3-K79 specific) Source: dictyBase

GO - Biological processi

  1. cellular response to DNA damage stimulus Source: dictyBase
  2. DNA repair Source: UniProtKB-KW
  3. histone H3-K79 methylation Source: dictyBase
  4. regulation of sorocarp development Source: dictyBase
  5. regulation of transcription, DNA-templated Source: UniProtKB-KW
  6. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Methyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, Transcription, Transcription regulation

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Histone-lysine N-methyltransferase, H3 lysine-79 specific1 Publication (EC:2.1.1.43By similarity)
Alternative name(s):
Disruptor of telomeric silencing A1 Publication
Histone H3-K79 methyltransferaseBy similarity
Short name:
H3-K79-HMTaseBy similarity
SAM domain-containing proteinImported
Gene namesi
Name:dotAImported
Synonyms:dot11 Publication, KMT41 Publication
ORF Names:DDB_G0271626
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0271626. dotA.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Individuals exhibit 4-5 hours delay in development. Development does progress with fewer fruiting bodies and smaller spore heads. No apparent changes in cell cycle regulation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18451845Histone-lysine N-methyltransferase, H3 lysine-79 specificPRO_0000413849Add
BLAST

Expressioni

Developmental stagei

Expressed during the whole life cycle.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi44689.DDBDRAFT_0216874.

Structurei

3D structure databases

ProteinModelPortaliQ55AX2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1125 – 1446322DOT1PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni625 – 63915Required for interaction with nucleosomes and DNABy similarityAdd
BLAST
Regioni1251 – 12544S-adenosyl-L-methionine bindingPROSITE-ProRule annotation
Regioni1274 – 128310S-adenosyl-L-methionine bindingPROSITE-ProRule annotation

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi37 – 7337Asn-richSequence AnalysisAdd
BLAST
Compositional biasi90 – 226137Ser-richSequence AnalysisAdd
BLAST
Compositional biasi121 – 16040Gln-richSequence AnalysisAdd
BLAST
Compositional biasi195 – 26369Asn-richSequence AnalysisAdd
BLAST
Compositional biasi336 – 449114Thr-richSequence AnalysisAdd
BLAST
Compositional biasi454 – 46916Asn-richSequence AnalysisAdd
BLAST
Compositional biasi464 – 676213Arg/Glu/Lys-richAdd
BLAST
Compositional biasi730 – 77142Asn-richSequence AnalysisAdd
BLAST
Compositional biasi832 – 86231Gln-richSequence AnalysisAdd
BLAST
Compositional biasi863 – 88018Thr-richSequence AnalysisAdd
BLAST
Compositional biasi965 – 1065101Asn-richSequence AnalysisAdd
BLAST
Compositional biasi1467 – 1659193Ser-richSequence AnalysisAdd
BLAST
Compositional biasi1489 – 150113Thr-richSequence AnalysisAdd
BLAST
Compositional biasi1521 – 155636Asn-richSequence AnalysisAdd
BLAST
Compositional biasi1772 – 184473Asn-richSequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.PROSITE-ProRule annotation
Contains 1 DOT1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3924.
InParanoidiQ55AX2.
OMAiLEISIME.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR013110. DOT1.
IPR025789. Histone_H3-K79_MeTrfase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF08123. DOT1. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51569. DOT1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q55AX2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTNSTPRKQ KLSNSKSLQN SPISPTVKKT NSFPLGNNIP TNINRSKKDK
60 70 80 90 100
NNNSNNNINN NNSNGIGSNT IINSTPIATT PVPPLPFIHS SSSSSSSPSP
110 120 130 140 150
SSSSSSPFPK AKKSPSLSIN QQQQQQPQQP QQSPQSQQSP QSQQSQQSQQ
160 170 180 190 200
QQPQQPQEQQ EPLPNLSFLR NQEPDKNVLP TSRKRPPSVM PSTPNQSSNS
210 220 230 240 250
SSLNSSLNFS SSNSSPSPTS TQSNNSRFET RSQNDQYENN NNNNNNNNNN
260 270 280 290 300
NNNNNNNNNN NNNIECIVID DDDDDDDDEG NSIKSTHTST QSTPIRDRRQ
310 320 330 340 350
RDNKWTINPL PQFQREIIDV DTPSPPNESL SIVSQTTTNT ITDTTSIQTP
360 370 380 390 400
TLIRQSSSLL SSSSSLSPST TSTPLTQNNI NLQNAQVIAT MTAPMEIELP
410 420 430 440 450
TIVQLEPLFS SEFTTSTQNL FIQTPPFTST LTLPTTTAQT SQTLFTIQTS
460 470 480 490 500
HDINNNNNNN NNNKNKNKNK KEIEKEKEKL REALKQKLKE YENENEKERE
510 520 530 540 550
KERKREREIE IERERKERER KEREKERKKE KEREREREER ERKEIERKER
560 570 580 590 600
EREEREERER KEIERKETER KEIERKEIER KEIERKERER KERKEREERE
610 620 630 640 650
EREREERERK EREEREKEIE MEREKKKEKE KEKEKEKEKE KEKEKEKEKE
660 670 680 690 700
KEKEKEKEKK RKENEVENEI EKERREKNDS YMVLNYHHSI DDHHSESESE
710 720 730 740 750
SDSDQDSIYS ISTQELSSVI SDNDFCDSDN EKVANNNRTV GETFLNDSRN
760 770 780 790 800
NNNNNNNNKN NNNKKIENDK NQLIERERLI SAFNDKAFLY ALDTIREVLG
810 820 830 840 850
GIDFKLKVSK EQIIEISTNA KKPLNILSEP EQQQQQQQQQ QQQQHQQQQQ
860 870 880 890 900
QQQQQQQQQQ QQTTKTTTTT NNTTTTTAET EKPKEVFLLK KPIKIPYGRD
910 920 930 940 950
CKKRLIRRFH GTSRNPLFHK NLESTLKLLK RAKFDWASIE FDTKSYLMNC
960 970 980 990 1000
NCKTVCHKSE RMKDNPTNKL QNNNRNNNNN NNNIINNNNN NNNKNNNNKN
1010 1020 1030 1040 1050
NNNKNNNRNN NSIAKKIGTN NNNNNTTIIK NNNNNNNNNN NNNNNNNNII
1060 1070 1080 1090 1100
KNNNNNNKNN NNNNNTIVKK IETIKKDINK KPTKTTTTTS SSSSSTSSSN
1110 1120 1130 1140 1150
SLTVIKKPVK KINGSQRICL FEDDFDVGIG VPVTTGTSET TTTRASSIRR
1160 1170 1180 1190 1200
KMNISNIFDD FTKKPRQNKY NEIEMPDLFA SKECNYTLEQ QAPFIRAQKL
1210 1220 1230 1240 1250
LLTQQHENNL YNGKRFMKYD EWLDLYHGEM RSSIQNPKVL KHYISFSQEV
1260 1270 1280 1290 1300
YGEAEPTLLR HWIHLGLIKP TDVFCDIGCG IGNVLFQLAA QVGCRVIGVE
1310 1320 1330 1340 1350
IRKDLYDISQ SMLEIYKKRS LELGLHPSTQ QIKIYNCDVK GSLEFDFSEP
1360 1370 1380 1390 1400
NVFFMHNTCF GPELEISIME LFKKYSKPGT KVITMKTLCP RFKPSDKKTK
1410 1420 1430 1440 1450
PWGIFKYPYE SYEMEEGSLS WRSATNCSFY SFTIDDKDSD IVTDQTHLNR
1460 1470 1480 1490 1500
VILSTPKKKH SKLQLFSSSS LPSSPPSSSS SSSPPNIATN TTTTTTTTTT
1510 1520 1530 1540 1550
TSPSSISLPS PYLSPSKKTP NSNKRDRSDI DNSNSDDGDE NNNNININNN
1560 1570 1580 1590 1600
NNNSNNKPIK LKLSMDHSID NQSNSESSDT DVEYMPWSKR NNRKKRKSLS
1610 1620 1630 1640 1650
YSLDSYLSSR ISPSLSLSTS SSSSSSLDSS PYSSPPSSSS SDNENDDDNG
1660 1670 1680 1690 1700
DDEDDSSSSN DTKLKEKLLL MKNNEKIGGA PPLTRRNANS DTNKLVQGCY
1710 1720 1730 1740 1750
QSLSSYALPK EESQIHKLQL QAKLLEHKNS LVLKHQKSIH DQQKRLSRKQ
1760 1770 1780 1790 1800
KKLAKKNKKK EQQLQAQAKT INYNNNNNNN NQNDNQVNHN NLNENEINTD
1810 1820 1830 1840
LINGYNNNNN NNIINNDNDN DNDNDKDDDK DSNNKDYNNI NDNNK
Length:1,845
Mass (Da):212,281
Last modified:May 2, 2006 - v2
Checksum:i6CDF92C0D67AAD87
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000006 Genomic DNA. Translation: EAL71677.2.
RefSeqiXP_645574.2. XM_640482.2.

Genome annotation databases

EnsemblProtistsiDDB0233511; DDB0233511; DDB_G0271626.
GeneIDi8618027.
KEGGiddi:DDB_G0271626.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000006 Genomic DNA. Translation: EAL71677.2 .
RefSeqi XP_645574.2. XM_640482.2.

3D structure databases

ProteinModelPortali Q55AX2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDBDRAFT_0216874.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0233511 ; DDB0233511 ; DDB_G0271626 .
GeneIDi 8618027.
KEGGi ddi:DDB_G0271626.

Organism-specific databases

dictyBasei DDB_G0271626. dotA.

Phylogenomic databases

eggNOGi KOG3924.
InParanoidi Q55AX2.
OMAi LEISIME.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR013110. DOT1.
IPR025789. Histone_H3-K79_MeTrfase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF08123. DOT1. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS51569. DOT1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "The histone methyltransferase Dot1 is required for DNA damage repair and proper development in Dictyostelium."
    Muller-Taubenberger A., Bonisch C., Furbringer M., Wittek F., Hake S.B.
    Biochem. Biophys. Res. Commun. 404:1016-1022(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
    Strain: AX21 Publication.

Entry informationi

Entry nameiDOT1L_DICDI
AccessioniPrimary (citable) accession number: Q55AX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2011
Last sequence update: May 2, 2006
Last modified: October 1, 2014
This is version 57 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

In contrast to other lysine histone methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3