SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q55AX2

- DOT1L_DICDI

UniProt

Q55AX2 - DOT1L_DICDI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Histone-lysine N-methyltransferase, H3 lysine-79 specific
Gene
dotA, dot1, KMT4, DDB_G0271626
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Histone methyltransferase that specifically methylates histone H3 to form H3K79me. This methylation is required for telomere silencing, correct growth and development, and for resistance to DNA damage induced by UV LIGHT.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei1300 – 13001S-adenosyl-L-methionine By similarityBy similarity

GO - Molecular functioni

  1. histone methyltransferase activity (H3-K79 specific) Source: dictyBase

GO - Biological processi

  1. DNA repair Source: UniProtKB-KW
  2. cellular response to DNA damage stimulus Source: dictyBase
  3. histone H3-K79 methylation Source: dictyBase
  4. regulation of sorocarp development Source: dictyBase
  5. regulation of transcription, DNA-templated Source: UniProtKB-KW
  6. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Methyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, Transcription, Transcription regulation

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Histone-lysine N-methyltransferase, H3 lysine-79 specific (EC:2.1.1.43)
Alternative name(s):
Disruptor of telomeric silencing A
Histone H3-K79 methyltransferase
Short name:
H3-K79-HMTase
SAM domain-containing protein
Gene namesi
Name:dotA
Synonyms:dot1, KMT4
ORF Names:DDB_G0271626
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0271626. dotA.

Subcellular locationi

Nucleus By similarity By similarity

GO - Cellular componenti

  1. nucleus Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Individuals exhibit 4-5 hours delay in development. Development does progress with fewer fruiting bodies and smaller spore heads. No apparent changes in cell cycle regulation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18451845Histone-lysine N-methyltransferase, H3 lysine-79 specific
PRO_0000413849Add
BLAST

Expressioni

Developmental stagei

Expressed during the whole life cycle.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi44689.DDBDRAFT_0216874.

Structurei

3D structure databases

ProteinModelPortaliQ55AX2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1125 – 1446322DOT1
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni625 – 63915Required for interaction with nucleosomes and DNA By similarityBy similarity
Add
BLAST
Regioni1251 – 12544S-adenosyl-L-methionine binding By similarity
Regioni1274 – 128310S-adenosyl-L-methionine binding By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi37 – 7337Asn-rich
Add
BLAST
Compositional biasi90 – 226137Ser-rich
Add
BLAST
Compositional biasi121 – 16040Gln-rich
Add
BLAST
Compositional biasi195 – 26369Asn-rich
Add
BLAST
Compositional biasi336 – 449114Thr-rich
Add
BLAST
Compositional biasi454 – 46916Asn-rich
Add
BLAST
Compositional biasi464 – 676213Arg/Glu/Lys-rich
Add
BLAST
Compositional biasi730 – 77142Asn-rich
Add
BLAST
Compositional biasi832 – 86231Gln-rich
Add
BLAST
Compositional biasi863 – 88018Thr-rich
Add
BLAST
Compositional biasi965 – 1065101Asn-rich
Add
BLAST
Compositional biasi1467 – 1659193Ser-rich
Add
BLAST
Compositional biasi1489 – 150113Thr-rich
Add
BLAST
Compositional biasi1521 – 155636Asn-rich
Add
BLAST
Compositional biasi1772 – 184473Asn-rich
Add
BLAST

Sequence similaritiesi

Contains 1 DOT1 domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3924.
InParanoidiQ55AX2.
OMAiLEISIME.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR013110. DOT1.
IPR025789. Histone_H3-K79_MeTrfase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF08123. DOT1. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51569. DOT1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q55AX2-1 [UniParc]FASTAAdd to Basket

« Hide

MSTNSTPRKQ KLSNSKSLQN SPISPTVKKT NSFPLGNNIP TNINRSKKDK     50
NNNSNNNINN NNSNGIGSNT IINSTPIATT PVPPLPFIHS SSSSSSSPSP 100
SSSSSSPFPK AKKSPSLSIN QQQQQQPQQP QQSPQSQQSP QSQQSQQSQQ 150
QQPQQPQEQQ EPLPNLSFLR NQEPDKNVLP TSRKRPPSVM PSTPNQSSNS 200
SSLNSSLNFS SSNSSPSPTS TQSNNSRFET RSQNDQYENN NNNNNNNNNN 250
NNNNNNNNNN NNNIECIVID DDDDDDDDEG NSIKSTHTST QSTPIRDRRQ 300
RDNKWTINPL PQFQREIIDV DTPSPPNESL SIVSQTTTNT ITDTTSIQTP 350
TLIRQSSSLL SSSSSLSPST TSTPLTQNNI NLQNAQVIAT MTAPMEIELP 400
TIVQLEPLFS SEFTTSTQNL FIQTPPFTST LTLPTTTAQT SQTLFTIQTS 450
HDINNNNNNN NNNKNKNKNK KEIEKEKEKL REALKQKLKE YENENEKERE 500
KERKREREIE IERERKERER KEREKERKKE KEREREREER ERKEIERKER 550
EREEREERER KEIERKETER KEIERKEIER KEIERKERER KERKEREERE 600
EREREERERK EREEREKEIE MEREKKKEKE KEKEKEKEKE KEKEKEKEKE 650
KEKEKEKEKK RKENEVENEI EKERREKNDS YMVLNYHHSI DDHHSESESE 700
SDSDQDSIYS ISTQELSSVI SDNDFCDSDN EKVANNNRTV GETFLNDSRN 750
NNNNNNNNKN NNNKKIENDK NQLIERERLI SAFNDKAFLY ALDTIREVLG 800
GIDFKLKVSK EQIIEISTNA KKPLNILSEP EQQQQQQQQQ QQQQHQQQQQ 850
QQQQQQQQQQ QQTTKTTTTT NNTTTTTAET EKPKEVFLLK KPIKIPYGRD 900
CKKRLIRRFH GTSRNPLFHK NLESTLKLLK RAKFDWASIE FDTKSYLMNC 950
NCKTVCHKSE RMKDNPTNKL QNNNRNNNNN NNNIINNNNN NNNKNNNNKN 1000
NNNKNNNRNN NSIAKKIGTN NNNNNTTIIK NNNNNNNNNN NNNNNNNNII 1050
KNNNNNNKNN NNNNNTIVKK IETIKKDINK KPTKTTTTTS SSSSSTSSSN 1100
SLTVIKKPVK KINGSQRICL FEDDFDVGIG VPVTTGTSET TTTRASSIRR 1150
KMNISNIFDD FTKKPRQNKY NEIEMPDLFA SKECNYTLEQ QAPFIRAQKL 1200
LLTQQHENNL YNGKRFMKYD EWLDLYHGEM RSSIQNPKVL KHYISFSQEV 1250
YGEAEPTLLR HWIHLGLIKP TDVFCDIGCG IGNVLFQLAA QVGCRVIGVE 1300
IRKDLYDISQ SMLEIYKKRS LELGLHPSTQ QIKIYNCDVK GSLEFDFSEP 1350
NVFFMHNTCF GPELEISIME LFKKYSKPGT KVITMKTLCP RFKPSDKKTK 1400
PWGIFKYPYE SYEMEEGSLS WRSATNCSFY SFTIDDKDSD IVTDQTHLNR 1450
VILSTPKKKH SKLQLFSSSS LPSSPPSSSS SSSPPNIATN TTTTTTTTTT 1500
TSPSSISLPS PYLSPSKKTP NSNKRDRSDI DNSNSDDGDE NNNNININNN 1550
NNNSNNKPIK LKLSMDHSID NQSNSESSDT DVEYMPWSKR NNRKKRKSLS 1600
YSLDSYLSSR ISPSLSLSTS SSSSSSLDSS PYSSPPSSSS SDNENDDDNG 1650
DDEDDSSSSN DTKLKEKLLL MKNNEKIGGA PPLTRRNANS DTNKLVQGCY 1700
QSLSSYALPK EESQIHKLQL QAKLLEHKNS LVLKHQKSIH DQQKRLSRKQ 1750
KKLAKKNKKK EQQLQAQAKT INYNNNNNNN NQNDNQVNHN NLNENEINTD 1800
LINGYNNNNN NNIINNDNDN DNDNDKDDDK DSNNKDYNNI NDNNK 1845
Length:1,845
Mass (Da):212,281
Last modified:May 2, 2006 - v2
Checksum:i6CDF92C0D67AAD87
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000006 Genomic DNA. Translation: EAL71677.2.
RefSeqiXP_645574.2. XM_640482.2.

Genome annotation databases

EnsemblProtistsiDDB0233511; DDB0233511; DDB_G0271626.
GeneIDi8618027.
KEGGiddi:DDB_G0271626.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000006 Genomic DNA. Translation: EAL71677.2 .
RefSeqi XP_645574.2. XM_640482.2.

3D structure databases

ProteinModelPortali Q55AX2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDBDRAFT_0216874.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0233511 ; DDB0233511 ; DDB_G0271626 .
GeneIDi 8618027.
KEGGi ddi:DDB_G0271626.

Organism-specific databases

dictyBasei DDB_G0271626. dotA.

Phylogenomic databases

eggNOGi KOG3924.
InParanoidi Q55AX2.
OMAi LEISIME.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR013110. DOT1.
IPR025789. Histone_H3-K79_MeTrfase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF08123. DOT1. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS51569. DOT1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "The histone methyltransferase Dot1 is required for DNA damage repair and proper development in Dictyostelium."
    Muller-Taubenberger A., Bonisch C., Furbringer M., Wittek F., Hake S.B.
    Biochem. Biophys. Res. Commun. 404:1016-1022(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
    Strain: AX2.

Entry informationi

Entry nameiDOT1L_DICDI
AccessioniPrimary (citable) accession number: Q55AX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2011
Last sequence update: May 2, 2006
Last modified: June 11, 2014
This is version 56 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

In contrast to other lysine histone methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3