Q559M9 (SYDC2_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase, cytoplasmic 2
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase 2
Short name=AspRS 2

Gene names

Name:	aspS2
ORF Names:	DDB_G0272304

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	569 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp).
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aspartyl-tRNA aminoacylation Inferred from sequence or structural similarity. Source: dictyBase
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: dictyBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from sequence or structural similarity. Source: dictyBase nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 569	569	Aspartate--tRNA ligase, cytoplasmic 2		PRO_0000327968
Regions
	Compositional bias	47 – 55	9	Poly-Asp
	Compositional bias	496 – 519	24	Poly-Asn

Sequences

	Sequence		Length	Mass (Da)
	Q559M9 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: FB20FE145096432F	FASTA	569	67,218
	10 20 30 40 50 60 MSEENNHKEK SKNEIKKEKK KIEKEKKITE LKSKKKINEK NNTINYDDDD DNDDDNFKNK 70 80 90 100 110 120 KWGEINNENY KEIIKNRFVK EWSNISDLNE SCVGKYVLIR ARVSNIRSFG NSLCFLQLRD 130 140 150 160 170 180 GLSSIQAVIS KNDENNSKSM IHFINSTITK ESIIDIEAIL TNSSTPIESC IIKNLELKIY 190 200 210 220 230 240 SLFLQSKSNS SLPLQFDDLS KPTTYQNYDD NGYTYVIPLN SRLNNRCLDL RTFYNLSIFK 250 260 270 280 290 300 IQSAISNLFR DQLLINDFIE IHSPKIIKQS INENSFKLNY FNEIAYLSES TQFYRQLAIV 310 320 330 340 350 360 SDFKRVFEIG PVYRTDLGHT HRHLNEFTSL DFEMTFKDHY HEVLDFLDNL MISIFKILET 370 380 390 400 410 420 NYENELKIIN NYQLQFEKFK FSTKTPRFTF SEVKLMLIEF SENENNKFCM ELFDYLNVQE 430 440 450 460 470 480 ERIFGKIVKE KFNVDYYIIE KPSSEYQPFY IMPDSNNEKL LNSFHIYING EKIGSGSQRI 490 500 510 520 530 540 HDWKLLEKRY KNYYINHNNN NNNNNNNNNN NNNNNNNNNI ENYINIFKFG CSQHAGCSIG 550 560 LERLVMAYLG LENIRKASFC PRDPTRLTP

References

[1]

"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

[2]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000008 Genomic DNA. Translation: EAL71304.1.
RefSeq	XP_645305.1. XM_640213.1.
3D structure databases
HSSP	HSSP built from PDB template 1X56 based on UniProtKB O57980.
ProteinModelPortal	Q559M9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q559M9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231309; DDB0231309; DDB_G0272304.
GeneID	8618471.
GenomeReviews	Gene locus aspS2 in contig CM000151_GR.
KEGG	ddi:DDB_G0272304.
Organism-specific databases
dictyBase	DDB_G0272304. aspS2.
Phylogenomic databases
eggNOG	KOG0556.
GeneTree	EPrGT00050000000242.
HOGENOM	HBG745843.
PhylomeDB	Q559M9.
Family and domain databases
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004523. Asp-tRNA-synth_IIb_arc/euk. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF10. PTHR22594:SF10. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYDC2_DICDI

Accession

Primary (citable) accession number: Q559M9
Secondary accession number(s): Q75JQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)